RATHNAVEL SUBRAMANIAM COLLEGE OF ARTS AND SCIENCE

(AUTONOMOUS)
SULUR, COIMBATORE-641 402
(REACCREDITED WITH A GRADE BY NAAC)

B.Sc. BIOTECHNOLOGY
LAB IN FORENSIC BIOTECHNOLOGY
COP PRACTICAL RECORD

2015-2016
Even semester

RATHNAVEL SUBRAMANIAM COLLEGE OF ARTS AND SCIENCE

(AUTONOMOUS)

SULUR, COIMBATORE-641 402

(REACCREDITED WITH A GRADE BY NAAC)

B.Sc. BIOTECHNOLOGY
LAB IN FORENSIC BIOTECHNOLOGY
COP PRACTICAL RECORD

2015-2016

NAME

___________________________________

REG.NO.

___________________________________

CLASS

___________________________________

RATHNAVEL SUBRAMANIAM COLLEGE OF ARTS AND SCIENCE

(AUTONOMOUS)
SULUR, COIMBATORE-641 402
(REACCREDITED WITH A GRADE BY NAAC)

B.Sc. BIOTECHNOLOGY
LAB IN FORENSIC BIOTECHNOLOGY
COP PRACTICAL RECORD

2015-2016
NAME

____________________

REG.NO.

____________________

CERTIFICATE
Certified to be the bonafide record of work done by ____________________ of III B.Sc
Biotechnology during the year 2015-2016 in the Department of Biotechnology.

Staff Incharge

Head of the Department

Submitted for the Practical Examination held on ___________ at RVS college of Arts and
Science (Autonomous), Sulur, Coimbatore.

INTERNAL EXAMINER

EXTERNAL EXAMINER

S.
No

Date

Name of the Experiment

Examination of saliva and saliva stains

Examination of blood and blood stains

Identification of species of origin

Examination of urine sample (Creatinine)

Clinical examination of hair

General characteristics of human hair

from different sites

Identification of common commercial

textile fibre

Examination and identification of

diatoms

Isolation of human genome DNA from

different samples collected from different
scenes
i.
Buccal sample
ii.
Hair sample
iii.
Blood sample

Page.
No

Signature of
the Staff

Examination of saliva and saliva stains- Radial diffusion method

a)

b)

Plate a and b show the zone of clearance in the starch medium by the presence
of amylase enzyme

Estimation of creatinine in urine

A = Blank
B = Standard 1 (2 mg/ml)
C = Standard 2 (4 mg/ml)
D = Standard 3 (6 mg/ml)
E = Standard 4 (8 mg/ml)
F = Standard 5 (10 mg/ml)
G = Standard 6 (Unknown)

Isolation of DNA from Human Buccal sample

Lane 1

Lane 1 and 3 show the DNA bands isolated from human buccal sample

Isolation of DNA from Human Hair and blood sample

Lane 1
4

Lane 1 and 2 show the DNA bands isolated from human hair sample
Lane 3 and 4 show the DNA bands isolated from human blood sample

Examination and identification of diatoms

a)

b)

c)

d)

Plates a, b, c and d show the presence of diatoms from the lake water sample
viewed under light microscope at 45X.