The effect of Gembili starch (Dioscorea esculenta) and Eubacterium rectal

Supplementation on Skeletal Muscle Peroxisome Proliferator Activated

Receptor Co-activator 1 (PGC-1) Expression in Diabetic Rat
Tri Setyawati1, Rio Jati Kusuma2, Harry Freitag Luglio2, Neni Oktiyani3, Sunarti2
Department of Biochemistry, Faculty of Medicine, Universitas Tadulako, Indonesia
2
Department of Health Nutrition, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
3
Departement of Biochemistry, Poltekkes of Banjarmasin, Indonesia
1

Introduction

Method
Hyperglycemia in type 2 diabetes mellitus (T2DM) triggers
Three months old male diabetic Wi
increased production of reactive oxygen species (ROS) which
rats were divided into groups based
lowers the biogenesis of mitochondria and causes
on dietary supplement: gembili
mitochondrial dysfunction.
starch only; gembili starch
At the mitochondrial dysfunction, expression of Pgc-1,
with Eubacterium rectal; and
especially in
Eubacterium rectal only. Positive (d
skeletal muscle and adipose tissues decreased. Pgc-1 have
rat) and negative (non-diabetic) co
important
groups were used in this study. Aft
role in oxidative phosphorylation in the mitochondria.
4 weeks of supplementation,
Lesser yam contain high of resistant starch that ca be
Pgc-1 expression in musculus vas
fermented
Objectives
lateralis were analyzed by
by colonic bacteria to produce short chain
fatty
acids
(SCFA).
The objective of this study Immunohistochemistry technique.
Butyric acid can improve mitochondrial
in diabetes
wasfunction
to investigate
the
of gembili
starch
mellitus through improving of genes effect
expression
such
as PGCand
gembili
starch
with
1.

Discussion:
We conclude that
supplementation of gembili starch
in combination with Eubacterium
rectal was able to improve fasting
plasma glucose level in diabetic
mice through amelioration of PGC-

butirogenic bacteria
Eubacterium rectal on
PGC-1 expression in
skeletal muscle of diabetic
rat

Result:

Tabel 1. Pgc-1 expression Kelompok

in skeletal muscle
REFERENCES:
K1
DeFronzo RA, Simonson D, et al. Hepatic
and peripheral insulin resistance: a
K2

K1
cotrol

health

K5
DM+sinbio

K3
DM+e.rectale

Rerata (%)

29,46 4,16a

9,84 2,59b