Anticarsia gemmatalis

cry1Ac/cry2Aa/cry9Bb
Table 1 Primers that recognize the cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab genes of Bacillus thuringiensis and the expected PCR amplicon
size
Ann.
Prod
temp
.
Gene
Sequence of primer (F)
Sequence of primer (R)
Reference
.
(bp)
(OC)
cry1

CTGGATTTACAGGTGGGGATAT

TGAGTCGCTTCGCATATTTGACT

558

cry1A
a

TGTAGAAGAGGAAGTCTATCCA

TATCGGTTTCTGGGAAGTA

272

cry1A
b

TGTAGAAGAGGAAGTCTATCCA

TATCGGTTTCTGGGAAGTA

284

cry1A
c

TGTAGAAGAGGAAGTCTATCCA

TATCGGTTTCTGGGAAGTA

272

cry1D

TGTAGAAGAGGAAGTCTATCCA

TGTAGAAGAGGAAGTCTATCCA

284

GTTATTCTTAATGCAGATGAATGGG

GAGATTAGTCGCCCCTATGAG

498

cry2

52
48

(Ceron et al., 1995)

48

(Ceron et al., 1995)

48

(Ceron et al., 1995)

48

(Ceron et al., 1995)

54

(Ben-Dov et al.,
1997)

cry2A
a
cry2A GGGATCCATGAATAATGTATTGAATAGTGG GGGATCCTTAATAAAGTGGTGGAAGATTAGT
b
AAG
TGGC

(Bravo et al., 1998)

(Lima, 2009)
199
0

52

(Lima et al., 2008)

Cry9A
a

GCTCAATCTGCTCAAATATCTGTT

TTTCACATTCTGTTTGAGAATCTTTT

54

(Alberghini et al.,
2005)

F = specific forward oligonucleotide primers; R = specific reverse oligonucleotide primers, Ann. Temp Annealing temperature ( oC).

Cry1 Ceron et al.,1995

5TGTAGAAGAGGAAGTCTATCCA3 (d)1
5TATCGGTTTCTGGGAAGTA3 (r)2

cry1Aa Ceron et al., 1994

5TGTAGAAGAGGAAGTCTATCCA3 (d)
5TTGGAGCTCTCAAGGTGTAA 3 (r)