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    19 Ansichten11 Seiten

    Rajaram Chapter I

    Hochgeladen von

    Rajaram Krishnasamy
    Book Chapter

    Copyright:

    © All Rights Reserved
    Als PDF herunterladen oder online auf Scribd lesen
    Markieren Sie unangemessene Inhalte
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    ernest recente ced ST Oe nats rated Biotechnological Approaches seg Sustainable Dye eyeyentants e i Bs bs. es a i a ag Fy Sac ae i si a es mr Incr ns ksstendeays Unverty, Pt Pula es Biotechnological Approaches for Sustainable Development Editor T. Pullaiah Department of Botany Sri Krishnadevaraya University “Anantapur-618003, AP, India 2015 Regency Publications ‘A Division of Astral International (P) Ltd New Delhi 110 002 rm th ede co a sin end kaa he veneer ray oe tee wy mel bs pala crime iran pc th ‘Tot toca dy rao ee ce Te oti gmk a sor Ra Tis rm of ym dh kf Theme stb ay cams arbres hho IM ore nk eri opi ot po hi nd, ‘aca paca ‘tne ova one re yay ae tor err ey ee “Gulag abicaton Dan-be ent OX Agnes OE canteen o> ite anes ssn ep i 2: Plut age erin «Cope : Lair ooe St Preface ictcaogy & expanding st phenomenal speed and there is every recent to catch Although tenet give al he infomation ti fiat ‘eine emai fo emer ou erat, May xpos ae working fr many year and acquiring knowledge on the ‘ltr research Theres every neces forthe experts © bring together Ail the information that they acurulaed over the pears. TR: book BENS together article by diferent experts on diverse topics like pro Imolcular matkers, DNA sequencing medina plants micropropagation ‘itopaopagation of plerkophytes and piytoremediation of Uranium. 1 request readers and researchers to give their suggestions for improvement of these articles and topics foe future edited books . Profe » Li of Contos & 1. Probiotics ~ Not just for your gut only: Impact en diseases beyond gt “Pongal 8 Raghacena 2 Molecular Mathers An Array of Technoogial Advancements 21 ‘nd Telr Applications in Crop Improvement “A. Chante Setar, P. Choire Obul Red, T.Kesbarcei, Ramet ant KN: Retna Rett ‘3 Insite reevaluation of DNA Sequences for Drawing 35 f Phylogeny of Orchids with Special Emphasis on Dealt Sw “Pritam Chtopangay ond Nima Bonroe 4 An Effective Nutritional Requiements and an in vitro. §3 “Approach for Asymblouic Seed Germination and Seeing {Growth of Some Terrestal Orchids Radha Majer ond Nirmala Bony |5. noite Approaches in Medina Pans-A Viable Statepy to 141, Base of Plant Based Systems of i. Bianca Apa fr Sustainable Dome | 6 Somaclonal Variation in Plant Tissue Culture and is Rolein 157 Crop Improvement =P Chandra Shear and 1, Fahne Naz 7. Insite Matipicaton of Poni Tepe (Sw) Holtam 169 “Aan Endangered Fern SM. Ison and VS: Monae A. Effect of Plant Growth Regulators on in ltro Raised 179 CGamtphytes of Phan aur I. Mt ons and VS. Manica 9 Aniulcer Activity ofan folated Compound (KR-1) from 189, Kampfria rt Lin. Lon Rats Prost Kionar Mira, Tanayn Gk, Pras Mire ant Gay Mira 10, ment of i Vitro Antioxidant and 201 city Morinda citi Fra ad 1, Remediation of Uranium Contaminatsd Soils: Conventional 211 and Emerging Technologies “Sieur Konda, Chanda Oba! Rady Pal, (nia Shr Ala, Vertue Pit, esr Kamar Cu, Jayne Nae Banach Sct of Candee, Dearie ort ena Given Kaap-s OA P, ih ‘dona, Pant Tout Catre Labora, Deprie o Seta tl Seces CAVE Camp TrnaithrSagmalcom 16, Hanamanharay, Pant Te Cure tary tama Unvesy of Agere Sere ovr in Suayanamayana, Pant Tw Caltre Laboratory, D atte Unter of Astral Sect, OF ‘entre 05 nia ee nin Oba Reddy Pal, Dpartnen of Plan Ssenes ote oot tena User, Vemarapra Kala inn Chant Setar Aka, Deparment Bosal: Sha tg Vahane Univer, Wemanapurin Kapa S16 cay Bhai Sigh Sigs 73008 iran ~ mp 1H, Fathima Nazneen, Department of Bots st as A ote Chapter 10 Comparative Assessment of In vitro Antioxidant and Antiproliferative Activity in Morinda citrifolia Fruit and Commercial Juice 1K. Rajaram and P. Suresh Kumar Aetroduction “Morinda citrifola (non!) used as an ienportant folk medline to treat various diseases, is Widely found in topical countries. Comparative Phytocherica screen in ult ae oat aie were attempt check ‘ficency. The alalid,Mavenoi, saponin, steroids and terpenoks Were resent high in dhe frit compared commercial juice, Boh rl a ue ‘were extricted with solvents (methanol, ethanol and water) and tested fF {ni antoxant activity (DPPH seavenging assay). Manimum peecenage of inhibition was observed only in the methanolic extract, fet le, £5350:07 ug/ml) and in juice IC 37970» 1.08) espectvely. Cell aby ‘say using normal col line (RAW) and andprferative act i liver mmo es en mea oe mer ‘thatthe concentration up to 10hig/ml have crease with higher dosages. So concentrations ‘of 25, 50 and 75 pg/ml wore slated forthe antprlieratve assay. The frat methanolic extracts (FME) exh significant (Cy, 19944090 g/l), anspeliferatve effect than juice methane extracts ME Cy 504923 748/ In. Tho outcome of ths study indented tat basline information fr he Diosctive potentiality of M. crc deel frit ever the commercial ce Morinda cleola L. ubiacene, commonly known as non, 4 smal cevergteen tee oF large bush that grows widely acroue Polynesia and ulated allover the woe (Kami a 208) I hae hen tracitonaly ‘sed for its antibacterial, antiviral, antifungal, antitumor, ant-helinth, nalgesi, hypotensive, aneinlnmatory, an immine enbancers fete (Wang of a, 2002) Furthermore, non je, which fe made from the pe {rai eas very popular because of ts many health benefits (Dison e ly 1998; Meclathey, 2003) |M. cifita Rubiaceae, Non) tat juice a wellknown heath dink uactioaly used as fk herbal remedy forthe trestment of many leases including diabetes, hypertension, and cancer. These effects have boon atecbued es net content which vieldeantonative and an Itlammatory ett (Hiranram ea 1996 Wang ac Su, 200; Kamiya a, 2004; Faas ef a, 2008) As previously reported, Non contains mary Srtioxdative and antiinflammatory ingredients such a, neoligaan, Smercanin A, vitamin C, vitamin E sopoltn and xeronine (Wang el, 20a; Kamiya et af, 2008), Renewed atenion in recent years to natural therapies has stimulated a new wave of research interest im triton practices, Hes have become a target forthe rare for new anticancer frags Abou half of the drupe used im inal practice come fom natal products (Butler, 2005). In fact, many nutritive and nonntetive Pytochemeale with diversified pharmacological properties hive shown Promising responses forthe provenion and treatment af various cance, Incing colon cancer (Swh, 2003) Inthe ast decade several papers have reported the chemicl constituents of noni and aon extracts revealing the presence of antaguinone (Kamiya eta, 20M), isdoid (Kamiya e aly 2004), avones (Deng al, 2007) gna (Deng ea, 207) and at ai Secvaives(Daultabed ea, 1985) and also the isogical activites sch fs hypotensive acivity (Younghen eal, 1960), ancancer activity (Wary {hd Su, 2001) and intbiton of copperinduced low-density pope {xidation (Kamiya etal, 2008). The fruit of Morinde ctrilia was commerciale as juices and sed for ny treatments. Hence ou present Study designed to astess the importance non dled fut and Non Commercial juice to study theltefficency in fn er antioxidant and 'ntproifeatve activity Hepatoclul aneet eal ine (Hep-G2) ‘Plant collection [Mafia fats wre procured fom the Local nurery and also non Ie ptt fm Se The as weil by Bona Say ‘of Ia, Coumttore, Tamil nad Ina (Authentication No= BSI/SRC/S 25/08-0/Tech 1369), 4 Phytochemical analysis - steroid, Pytochemicls sch a altalods, phenol, favo tecpendids and fnnine were analyzed. folowing the ‘proton (Gisborne, 1973; Treas and Bear, 1996) nam Preparation of Extracts ‘The fats were chopped in pee (em) and alt i i frat Gy) were porte ae exacted wy 2 see ete ‘tana ok seb) separaey lng meget es aE a ‘Shore tera ining Wetman ae poper Nols Foe oe of nor ue wee taken in sears fra and ed StS ca an cy Me a ie ‘to eel removed and Seed ining Wha ie: paper No The ‘sl ad vr cosa art, Jpus) whet m Uw aqccu cA Yaa Co Lat te conceal tac were dlclved DSO? sowie mae! cnt tape wane DPPH radicalscavenging effect ann ‘The radical savenging activity ofall extracts (rut Methanol (ME), Frat Ethanol Extract, Frat Aqueous Ext, Juice Methanol (IME, Juice Ethanol Extract and Jule Aqueous Extract) of Meni luted sing DPPH Soares a, 1997) Raa severing {DPPH = (Control OD” sample OD/Centrol OD) «100% Fach fssyed in tplcte for each concentration. Acorbie acd Was tad a ‘onto Cat iabilty and ant prairie amaye 2 14 vio nd Hepat pro) a wee soa a en te ab tl ov tml peat 37°C a hum rnp of 8 ‘simowrs! Sem theta 10% fetal cal ser, 90 Uo “Vetoes was sod in sig Plates in the gular growth medium. After incubation for 24 he els ‘rere maintained inthe med containing methane extract of NE tl fruit commercial juice and standard for 24 hat 37°C (8% CO;). Aloe Incubation, the spent medium was scared acl were was once ‘with DMEM aed resuspended ines miu (200/wel). g/l NITE (Gout /wel) was added wo calls, fellowed by incubation for 4h a 37°C (COs). The formazan crystals ere slublized by incubating the al with ‘DMSO‘150ig/ wel), The absorbance othe scltion was mesure at SOR sing a microplate reader (Molecular devices, France) The percentage of live els was determined ing the forma (Acree Ac Res Phytochemical screening Pytochemical analylsinlcated the presence of abundant amount of alkaoits, saponins, sterids, erpeolds and Navanoids components iM "flr rls compare to commercial ie (Table 10:1). This may be de {oles of some potential phytochemical Components during ce processing ‘le 11. Pinay syste ype nt he mare ec Fe 101. OPPH Radel seaengny ay fan Caneel cn wc Fe Fut art Enc FF ered Eo FA Frat Aon a ‘i ‘ce tebees aac EE ce Eh ac 5c gen aa ie re pers ate near #30 ‘le 102. DPPH too asl saver acy of mato, eat and Wt ‘sec om convo ice She | Prvecreateas [Fa camer ue = = i ai a 5 om oP arg ar z om cs . e a ore 7 on a z ee ‘onsooas ¢ tise J c 2 a ont @ = 5 o 7 Me “Wooo r er = =a 5 a “eo (i 6 yt 9 Fy rae (9 = = a Radical scavenging activity ating DPPH method : 5 co “There wos nea incense in the adic scavenging. actity ofall a cera with increment ofthe sample concentrations (101). PME (Ig 53502077 g/m) and JME (IC, 39970 = 1.03 g/m) showed sgnica nioxidant att than oer fat extracts tested (Tale 10.2). Hence he ‘methanol extract ofboth fruit and juice selected for atprolierativ acti, Cal vaity an ant-pieav amaye hel iy of FE a ME in RAW ci ew se ct omc os ipa enna ‘trot of thence wore npr it he err oa inca! Appa for Suna Despre RAW cel) The ress ofthe cll viability showed, that all extats have leserfoly upto 100 pg/ml concentration Fi, 102) Pa 103 Ce wy ety t mae ect a ar a Ant-prolferatve atity war performed in FME, JME and compared swith standard. The ICy of FME showed ranimam acivity (IC 18942090 Ing/ml) and comparale withthe standard (Cy value 153130 74hg/ml) Howover, JME shoved. higher values, (IC 80.8853.%yg/mi) (Fg. 103), Schatten | pave Assent of ite Anta Discussion i, Pye aa evel he prominent lene een the phon eompestions presen te frat and commer leg Ti rence of move pryiodecls inthe fat might be reponse nina andodat and ant polierae acy than oneal Antioxidants are the compounds which helps to delay or inhibit the oxidation of Upids and ter nlecls though the nen fe Intiaton or popepaton of tldatvechaln taco Gel e l 209 fotos tart weber mca aentso by Bs el cr ninget oxygen eects, Fee sedans cote fo mone funded cuoders in humane Tnloding shorn hy nd {Schema a reperion iy of ay tea cel evo Injury gant cancer an AIDS (Cook and Seman, $996 Kaplan and Salen, 197 Fee ras are ofr generted at ypeodie a Biolog actos or from exoenow factors. The ivaveaet te fails Ia the pathogerents oft large number of duces a8 Wel ‘twenty Pound a (2), Medical pans an farm effec of losing radaton, Netucl plan exe OF Compounds are se ngredints which do ot have any igh Woe Flam extcts can be characterized by polyvalent ormalaona And Inrpoted sate orn some cn, poten Pe the enuf of mii plans smal tru th nox fd oxave scat promise fete ofthese aes OE 2s Aboutwent ls 20 ‘DPPH ics sable feral that acepts an lection of rail and becomes sable danagnetc molecule ours 1 (tls), A deep purple car with an elorpon suo At ‘s‘ormed from BFPH solution, but Ie generally fades When 48 Anvondars ate preset inthe stn. iad anon AA ‘Rta liner eltnehip seth th eal peri or andoeya cot Soe pnts (Kal af, 199), which canbe validated By the fing ‘euch Our res sly inte tat he FE fe NR ano {itty among all ster exact A song postive creation has Bae ‘widespread groups 1,200). Therefore the presen of aon alba, ero terpenoide content in methane extrac of fruit might account eter seavenging effet on DPPH. Phenolic which from plant resources have = Bisco Appa for Sistine Deetyment aciviy and maily attribute anoxidant activity to thelr ex properties (Osea, 194: Sieeardhana a, 2009, ‘The preent study demonstrates the methanalic extract of ML ci fruits ponseed scant anbxidant ant antprolierative activity when ‘compared to commer juice. Thin may be due othe presence of rg [amount of phytochemicals such a alkaloids saponin sera epenoid present in the fat is responsible for the momentous antioxidant and Snuprolrative activity. Some of the phytochemicals may be lost dung omer ce processing and whch ls Very well correlate with reduced bone Acknowledgement ‘The authors thank DSTSERC, New Delhi, India far providing the financial sesitanee 4 cary out this work. References Aboutwent A. Pemberton PW, Soh A, Barons, PC and MeMaon, RET: ‘a. Osidantsteess fs signet feature ot petmary ilary ethos occ et Bopha Act Molecular Hass of Osease 163 14213 Sut MS. 5, Nt prac pel at dhe ome Coke, A, Maw A Yin A Dura MLE, Harmundar Man Kaze, C2008 Tolaon and shracterzaton of antxidant pheclic compounds rm te tach prt of pprtan pep by sy pale frctoneoal ‘Ehnogharmacl 7 7st Cook, NC and Summa, 5. 1996 Flavonoids chemistry, metabo, cndio Protcive eet and itary sures Nuon! Bch 76676 Dotted, CD. Mul, GML and Mir, AML 1988 ipl acl ‘ria seed lJ OM Teshnlopst Asoction of Inia 22627 Deng 5, Pals, AK, West. Bly Su CX, Zhou, BN. and Jensen J: Tlpcecypmans litte consiers ft Ee of nod roel ‘ceed in Tat J Nat Dre 7 8 Dh, AR, MeMiln Hand Atk NL 199. Ferment hs: he “non a taditonl Plynesian medicine (Morin ira, Radic Economic Botan 85-8 ahr J. Lngys Gand Bazovics, A 1998 Oniatv stress in the Be "any tc Sener Scandinavian Joumal 6! Cstontelg)» SUP ibs iss eel, CA Gop Manivanan,P. Son “owe pena and sain a tment wh girlie at) Bch S00) THEI Kak, MeDonl, JE Rie, RD. and La X. 19m. Anhoxyanin Pol wii and ann tibet speci. Canaan Pat Sk Kara, K, Hamate W. Harada S, Makan R, Tokay 288, Chel sentient Mornin eft oot exh ‘tle in reptnnaclinduce det ice. Bog ad Sates sat Re ami, Taal, Enang HL, Une, M. and Sala, 0 onatucns of ers cf its ib coppee inde ‘opm kon fApie Fool Chem SE SSS ‘ampulainn, [Tan Sloen J. 137. Natl eons a ‘Nuit, bath and aos The opal Sec ‘oo, AY. Ja Xan Da, LB, Anon nd dl Sf the pyrligous aid om a mangrove Pant RAS ‘Chem. S37 MeCinchey, W 202.FrmFlyesan healers ath fo pei firing ing Co OM, T, Masuda, M., Fra, Niki, Tara Nand Sd Tnvolement of anthocysnine ad other phenol compound Ik ‘avenging aby of priest oa aa a Ge ‘Osan T. 198, Novel utr! antoidnts for uation in oad a gl Spc: Urn Cat, Vand Nene EM ee PO Pa | ‘ocemisry of plant fod muir inthe Wop Jan Sete remap, pest avi AD. Su BN. Jung HA, Keer, WJ, McLaugh J and ‘A Sn Chantal sian i a re i ‘ir aodanacbty J Rat Po 6 58, Pourmorad.F, Howser, Sand Shaan N 3006 Annan nol and Maer cots lest nn medal lar Pauses , Soman tn. Kim Sh 1 Jo. 2, inn ci of HER arom ect oxygen tac, Perot ibion Teed Scene and ete Stewart, SSK and Sha 202. Antal att of ino an byproducts [Aran Of Chemis Sexe 9 20 srg Arps or Sutale Delmont “rome, GE ad Evan W196 Phenol and penalizes Ir: Toe and ‘rare Phamacognoy Biles Tindal Pliers, london, ‘Wang, MEY, Nowicki D. and Anderon, . 2002 Protective effect of Nerinde Hil on hepa sry iced ya lve carcinogen The Poedingy of Set Anal Meeting of Amer Asacaton for Caner Resch € 47 Wang. MY. and Su, C201. Cancer preventive tof Marin cra (Non "Rana of the New York Academy of Snes 9261-16 ‘Younghen, HN, Jens, HJ, al Baber, CL 1 Stes om Movida cri TnI, Ame. armas Sosoaton $9 271278 1 ners Chapter 11 Remediation of U; Contaminated Conventional and ‘Technologies Sravani Konduru, Chandra Obul Reddy Puli Akila, Varakumar Pandit, Krishna Kumar G Naik Banavath a Jens of nil wut ar © prong, Ue ter

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