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Purify
ChromatographyLPLC(=LowPressureLiquidChromatography)
referstoaprocessworkingatlowpressure(<6bar)allowinga
transferofmoleculesbetween:
Asolidstationaryphase,oftencalledchromatographymedia
orgel,chosentointeractwiththeproducttopurify
Aliquidmobilephasewhichreferstotheproducttopurifyor
anychemicalsolutionwhichconditionsthestationaryphaseto
adsorbordesorbthemolecules.
Thestationaryphaseiscontainedinachromatographycolumn
between2distributorswhichcontainsthestationaryphaseand
distributesevenlythemobilephasethroughtheentirecolumn
surfaceandtransferitindepthfromtoptobottom.
TheLPLCisjustifiedinthefollowingcases:
Fineseparationofhighvaluemolecules(>2$/ginorderof
magnitude).
Technologysuitableforseparatingmoleculesofsimilarsize,wherefiltrationcantwork
Technologysuitableforfragilemolecules,incompatiblewithprecipitationorhighvelocity(shearstress)
Canpurifyandconcentrate,inBind&Elutemode:thevaluablemoleculessometimesverydilutedin
themothersolution,endupcontainedinthebedvolumeafteradsorption.Theycanbeelutedin25
columnvolumes.
ThechangeofsolventinBind&Elutemodecanhelpfurtherstep.ExampleinReversePhase
Chromatography:loadrawproductinwater,elutethetargetmoleculesinEtOHthatcanbeeasily
evaporated
Adaptabletoanykindofmolecules:nucleicacids(DNA),proteines,glycoproteins,sugars,Lipids,
Youhaveaprocesschromatographyapplicationandlookafteranequipment(column,system,other):click
here.
Youneedassistancefordefiningyourpurificationprocess:clickhere.
SeehowtoClean
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