____Name__________________________

Lecture
Yr, Sec, & Course _______________
Transcription
I.

General Biochemistry
Prelims Quiz # 5: Replication and

Modified True or False.

Write A if only the first statement is true.
Write B if only the second statement is true.
Write C if both statements are true.
Write D if both statements are false.
1. The key enzyme that plays a major role in DNA replication is a RNA-directed DNA polymerase.
DNA polymerase III requires a RNA primer to start synthesizing a new DNA polynucleotide chain.
2. DNA synthesis is unidirectional in most organisms.
Topoisomerase unwinds the DNA for replication while the helicase reduces torsional stess brought
about by the unwinding of the DNA.
3. The leading strand in the DNA replication runs from 5 3 direction while the lagging strand runs
from 3 5.
Okazaki fragments are short DNA segments synthesized in the lagging strand.
4. Replication of the lagging strand has to be carried out in a semicontinuous manner.
RNA primers are excised and are replaced by deoxyribonucleotides by DNA polymerase I.
5. Semiconservative nature of replication is observed in all organisms.
Eukaryotic mRNA is synthesized in the nucleus by transcription and carries the genetic message to
the site of protein synthesis.
6. If the sequence of mRNA transcript if 5GCCUUA3, then the sequence of DNA coding strand is
5..GCCTTA..3.
The coding strand of the DNA is used as a template for the mRNA synthesis.
7. Pribnow box is a promoter region within base sequences of mRNA that can be found 10 nucleotides
downstream from transcription start site.
The sigma subunit of the RNA polymerase recognizes the promoter region in the DNA strand.
8. The first nucleotide used in the RNA synthesis contains pyrimidine bases.
The first nucleotide is incorporated into the RNA chain at the transcription start site, which is found
at position 0.
9. The RNA polymerase requires several primers for the synthesis of RNA.
Synthesis of mRNA runs from 5 to 3 direction.
10. Eukaryotic mRNA is fully processed in the nucleus before it is transported to the cytoplasm and
translated into protein.
Capping at the 3 end, polyadenylating at the 5 end and splicing of exons are modifications that
take place in primary mRNA transcript.

II.

Using the genetic code, answer the following.

1. If a DNA strand has a sequence of: 5'ATGTTTCGTTAAAAG -3, what will be the transcribed
mRNA?

2. Determine the amino acid sequence for this short

mRNA transcript. Indicate the N and C terminal of the
polypeptide chain.