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Brooke Bruder

Period 3
PGlo Lab Conclusion
In the PGlo lab, we tested if different food sources affected if the bacteria glows
or grows. Our hypothesis was that if there is a connection between food source and glow,
when all of the samples contain the same bacteria, by moving them to plates with // food
source, they will all react equally by glowing, and removing D from // will cause it to
stop glowing. Out hypothesis was partly correct. There was a connection between food
source and glow, there had to be arabinose. When moved to //, C and D glowed. This
was because arabinose was added to the bacteria. But A and D did not glow because
there was no arabinose. By removing D from // and moving it to /, it did not glow.
In the bacteria (HB101), there was a plasmid containing the GFP gene (the gene
that allows the bacteria to glow) and BLA (the antibiotic resistance gene). The food
source in the // contains LB, ampicillin, and arabinose, and the AraC, which is a
regulatory protein. The AraC attaches itself to the arabinose, which then turns on the GFP
gene in the plasmid causing it to glow. The reason the bacteria did not glow in / was
because there was no arabinose, only LB and ampicillin. The AraC does not have
anything to attach itself to turn the GFP gene on. This is the same with no dash, because
there was not arabinose present.

Brooke Bruder
Period 3
In order for the bacteria to grow there needs to be a plasmid in the bacteria with
the correct food source, LB and arabinose. Without food, it would not be able to grow at
all. Along with the food, there is also ampicillin, which is the antibiotic that would kill
the bacteria. If there is an antibiotic that would kill the bacteria, in order to stay alive
there would need to be BLA, which is an antibiotic resistant. This allows the bacteria to
continue growing because the antibiotic is not killing it.
The significance of this lab was to determine what turned the GFP gene on. When
the GFP gene is on, this caused the outbreak of zombies. If there was a way to turn off
the gene, we would be able to fix the outbreak. Therefore the purpose of the lab was to
figure out what the gene needed to turn on and to stay alive, which we determined was
the AraC attaching itself to the arabinose, and needing the BLA (antibiotic resistant) to
keep the bacteria alive from the ampicillin. Now that we know what is causing the
outbreak, we can fix it.
During the lab, one thing I learned was how a gene could turn on another gene.
Like how AraC attached to arabinose to turn on the GFP gene. Something like this
caused glowing of bacteria. This could be used in real life to find cancer. If scientists
could get cancer to glow, it would be easier to track and get rid of, because it would be
visible. One error that could have occurred during our experiment would be
contamination during streaking, for example, leaving the lid off for too long, or breathing
on the plate. These errors could have caused us to receive different data.

Brooke Bruder
Period 3
Works Cited
Donley. "Guided Investigation: Research and Conclusions." PGlo. Monarch High School.
Nov. 2015. Lecture.
"Image." GFP Gene Regulation in PGlo. N.p., n.d. Web. 15 Oct. 2015.
"Power Point." N.p., n.d. Web. 15 Oct. 2015.
https://app.schoology.com/docviewer/652270707/8dd259e633ff25781fc1c75142ce7fe6>.