BOI207

GENERAL MICROBIOLOGY
PRACTICAL 3: ASEPTIC
TECHNIQUE
PRACTICAL 4: PURE CULTURE
TECHNIQUE

NAME: SALIHAH BINTI ZAKARIA

STUDENT ID: 126197
GROUP: 14

PRACTICAL 3: ASEPTIC TECHNIQUE

OBSERVATIONS
A- Broth to broth
B- Slant to broth

C- Broth to plate

QUESTIONS
1. Yes, something grow in all of my transfer. Both of the broth turn cloudy while
the plate show E. coli grow on the plate after been transferred for one day.
2. Yes, my agar plate appear to be contaminated. There was a presence of white
colonies outside of the streaking line.
3. If in the broth cultures have showing other color than the cloudy, the broth
would had contaminated with a microbe other than E. coli.

PRACTICAL 4: PURE CULTURE TECHNIQUE

OBSEEVATIONS AND QUESTIONS
1. Streak plate method
Clear isolation of red S. marcescens and
white E. coli

E. coli from streak plate method

2. Pour plate method

Pour plate 2.
Growing on the medium (surface colonies) red S. marcescens
Growing within the medium (subsurface
colonies) - E. coli

S. marcescens from pour plate method

3. Streak plate method showed the best separation of species.

4. E. coli able to grow both on the surface and within the medium. Colonies are the
larger at the location 1. Because the early step, the microorganisms still not
separated from each other before being thin out.
5. Colony is a grouping of individuals of the same species rising from a single
bacteria cell. Colonies are genetically identical as they are formed from a single
cell that replicates and grows outward.

6. The advantages of streak-plate method:

The original culture is diluted serially and a small volume of the final
dilution is spread on the surface of an agar plate. A dilution gradient is
established across the face of the petri plate as bacterial cells are deposited
on the agar surface.
Spread millions of cells over the surface.
Individual cells deposited at a distance from all others;
Divide forming distinct colonies.
Distinct colonies do not touch any other colonies

7. Agar must first be cooled to 50oC to prevent condensation which could cause
moisture on the agar surface allowing for the organisms to spread over the surface
resulting in a faulty isolation process
8. 2 reasons the plate should be inverted during incubation:
We label the bottom surface of the agar plate. It is easier to find the plate
when it is inverted.
To reduce possible condensation on the agar surface