Scribd Logo
Hochladen

Willkommen bei Scribd!

  • Carbohydrate Postlab

    Hochgeladen von

    Thea Mallari
    21 Ansichten33 Seiten
    This document discusses carbohydrate tests. It describes several common tests used to identify different types of carbohydrates, including the Molisch test for overall carbohydrates, the iodine test for starch, Benedict's test for reducing sugars, Barfoed's test to distinguish between mono- and disaccharides, and the mucic acid test for galactose. It also outlines tests that can identify specific carbohydrate subgroups such as the Bial's orcinol test for pentoses and the Seliwanoff test for ketohexoses. The document provides the reagents, expected positive results, and carbohydrates detected for each test.

    Originalbeschreibung:

    Originaltitel

    carbohydrate-postlab.pptx

    Copyright

    © © All Rights Reserved

    Verfügbare Formate

    PPTX, PDF, TXT oder online auf Scribd lesen

    Stufen Sie dieses Dokument als nützlich ein?

    Sind diese Inhalte unangemessen?

    Dieses Dokument melden
    This document discusses carbohydrate tests. It describes several common tests used to identify different types of carbohydrates, including the Molisch test for overall carbohydrates, the iodine test for starch, Benedict's test for reducing sugars, Barfoed's test to distinguish between mono- and disaccharides, and the mucic acid test for galactose. It also outlines tests that can identify specific carbohydrate subgroups such as the Bial's orcinol test for pentoses and the Seliwanoff test for ketohexoses. The document provides the reagents, expected positive results, and carbohydrates detected for each test.

    Copyright:

    © All Rights Reserved
    Als PPTX, PDF, TXT herunterladen oder online auf Scribd lesen
    Markieren Sie unangemessene Inhalte
    21 Ansichten33 Seiten

    Carbohydrate Postlab

    Hochgeladen von

    Thea Mallari
    This document discusses carbohydrate tests. It describes several common tests used to identify different types of carbohydrates, including the Molisch test for overall carbohydrates, the iodine test for starch, Benedict's test for reducing sugars, Barfoed's test to distinguish between mono- and disaccharides, and the mucic acid test for galactose. It also outlines tests that can identify specific carbohydrate subgroups such as the Bial's orcinol test for pentoses and the Seliwanoff test for ketohexoses. The document provides the reagents, expected positive results, and carbohydrates detected for each test.

    Copyright:

    © All Rights Reserved
    Als PPTX, PDF, TXT herunterladen oder online auf Scribd lesen
    Markieren Sie unangemessene Inhalte
    von 33

    CARBOHYDRAT

    ES

    MONOSACCHARI
    DES

    DISACCHARIDES

    DISACCHARIDES

    DISACCHARIDES

    STARCH

    STARCH

    MOLISCH TEST
    Reagen
    ts:
    (+)
    visible
    result:
    Test
    for:

    Concd H2SO4, naphthol


    Purple color at the
    interface
    carbohydrates

    MOLISCH TEST

    http://www.esu.edu/~scady/Experiments/Carbohydrates%28summer

    IODINE TEST
    Reagen Iodine-KI solution
    ts:
    Blue-black solution
    (+)
    visible
    result:
    Test
    Starch
    for:

    BENEDICTS TEST
    Reagen
    ts:
    (+)
    visible
    result:
    Test
    for:

    CuSO4, Na2CO3, Na
    citrate
    Brick-red ppt (Cu2O)

    Reducing sugars:
    monosaccharides
    disaccharides

    BENEDICTS TEST

    BARFOEDS TEST
    Reagen
    ts:
    (+)
    visible
    result:
    Test
    for:

    Cu(CH3COO)2, acetic
    acid
    Brick-red ppt (Cu2O)

    Monosaccharide (<3
    mins), disaccharides
    (~10 mins)

    MUCIC ACID TEST


    Reagents
    :
    (+)
    visible
    result:
    Test for:

    Hot concd HNO3


    Broken glass-like
    crystals (galactaric
    acid)
    Galactose

    MUCIC ACID TEST

    Biochemistry
    By Debajyoti Das

    BIALS ORCINOL
    TEST
    Reagent Orcinol, FeCl3, HCl
    s:
    (+)
    Bluish-green solution
    visible
    result:
    Test for: pentoses

    SELIWANOFF
    TEST
    Reagents Resorcinol, HCl
    :
    (+)
    Cherry-red solution
    visible
    result:
    Test for: ketohexoses

    PHENYLHYDRAZONE
    TEST

    Biochemistry
    By Debajyoti Das

    Reducing sugar + phenylhydrazine =


    phenylhydrazone
    Phenylhydrazone + 2 phenylhydrazine =

    Laboratory Manual for Practical Biochemistry


    By Y. M. Shivaraja Shankara

    GLUCO

    FRUCTO

    MANNO

    Laboratory Manual for Practical Biochemistry


    By Y. M. Shivaraja Shankara

    Laboratory Manual for Practical Biochemistry


    By Y. M. Shivaraja Shankara

    NELSON ASSAY
    Reagents:
    Nelson A: Na2CO3, NaKtartrate,
    NaHCO3, Na2SO4
    Nelson B: CuSO45H2O, H2SO4
    Arsenomolydate reagent:
    (NH4)2MoO4, H2SO4,
    NaHAsO47H20
    (+)
    Blue color of complex

    Das könnte Ihnen auch gefallen