(Peukert, Thiel et al.

2014) studied the spatio-temporal dynamics of fructan

metabolism in barley grains using a metaomics approach that included: imaging
mass spectrometry, targeted metabolomics and transcriptomics. They analysed
developing barley grains at 3, 7, 10, 14, 17 and 20 days after pollination and
found different patterns of sugar distributions on the tissue. Levan- and
graminan- tyoe oligofructans were found in the cellularized endosperm before
starch biosynthesis, whilst inulin-type accumulated in and around the emerging
endosperm cavity. The changes in the fructan distributions were dependent on
the endosperm formation and grain filling. Such patterns were not observed on a
seg8 endosperm mutant.

(Seaman, Flinders et al. 2014) studied how atoms from dead plants are
incorporated into new life by plants. They hydroponically grew radish plants
using a nutrient system containing 15N KNO3 as the sole nitrogen source. Radish
plants were cropped and left to ferment in water to create a radish tea. The
latter was then used as the nitrogen source to hydroponically grow a second
batch of radish plants. Cryosections were obtained from harvested radish plants
and analysed using MALDI-MSI and SIMS-MSI. Glutamate, glutamine and arginine
were primarily investigated; however, a clear identification of these amino acids
turned out difficult due to complex overlapping isotope peaks of the positive-ion
mass spectrum. They were able to identify that choline and phosphocholine had
incorporated the labelled 15N, and that they were localized predominately in the
center of the radish bulb.. Approximately 50% of the material from the first
generation radish was transferred and assimilated by the second generation
radish.

(Becker, Carre et al. 2014)

(Gemperline and Li 2014)

(Horn, James et al. 2013)

(Horn, Korte et al. 2012)

(Horn, Silva et al. 2013)

(Horn, Sturtevant et al. 2014)

(Li, Hansen et al. 2013)

(Miquel, Trigui et al. 2014)

(Shroff, Schramm et al. 2015)

(Velikovi, Ropartz et al. 2014)

(Ye, Gemperline et al. 2013)

(Fraige, Gonzalez-Fernandez et al. 2015)

(Franceschi, Dong et al. 2012)

(Gemperline, Jayaraman et al. 2015)

(Liang, Sham et al. 2013)

(Schad, Mungur et al. 2005)

(Schiebold, Tschiersch et al. 2011)

Becker, L., V. Carre, A. Poutaraud, D. Merdinoglu and P. Chaimbault (2014).

"MALDI mass spectrometry imaging for the simultaneous location of resveratrol,

pterostilbene and viniferins on grapevine leaves." Molecules 19(7): 1058710600.

Fraige, K., R. Gonzalez-Fernandez, E. Carrilho and J. V. Jorrin-Novo (2015).
"Metabolite and proteome changes during the ripening of Syrah and Cabernet
Sauvignon grape varieties cultured in a nontraditional wine region in Brazil." J
Proteomics 113: 206-225.
Franceschi, P., Y. Dong, K. Strupat, U. Vrhovsek and F. Mattivi (2012). "Combining
intensity correlation analysis and MALDI imaging to study the distribution of
flavonols and dihydrochalcones in Golden Delicious apples." J Exp Bot 63(3):
1123-1133.
Gemperline, E., D. Jayaraman, J. Maeda, J. M. Ane and L. Li (2015). "Multifaceted
investigation of metabolites during nitrogen fixation in Medicago via high
resolution MALDI-MS imaging and ESI-MS." J Am Soc Mass Spectrom 26(1): 149158.
Gemperline, E. and L. Li (2014). "MALDI-mass spectrometric imaging for the
investigation of metabolites in Medicago truncatula root nodules." J Vis Exp(85).
Horn, P. J., C. N. James, S. K. Gidda, A. Kilaru, J. M. Dyer, R. T. Mullen, J. B.
Ohlrogge and K. D. Chapman (2013). "Identification of a new class of lipid
droplet-associated proteins in plants." Plant Physiol 162(4): 1926-1936.
Horn, P. J., A. R. Korte, P. B. Neogi, E. Love, J. Fuchs, K. Strupat, L. Borisjuk, V.
Shulaev, Y. J. Lee and K. D. Chapman (2012). "Spatial mapping of lipids at cellular
resolution in embryos of cotton." Plant Cell 24(2): 622-636.
Horn, P. J., J. E. Silva, D. Anderson, J. Fuchs, L. Borisjuk, T. J. Nazarenus, V.
Shulaev, E. B. Cahoon and K. D. Chapman (2013). "Imaging heterogeneity of
membrane and storage lipids in transgenic Camelina sativa seeds with altered
fatty acid profiles." Plant J 76(1): 138-150.
Horn, P. J., D. Sturtevant and K. D. Chapman (2014). "Modified oleic cottonseeds
show altered content, composition and tissue-specific distribution of
triacylglycerol molecular species." Biochimie 96: 28-36.
Li, B., S. H. Hansen and C. Janfelt (2013). "Direct imaging of plant metabolites in
leaves and petals by desorption electrospray ionization mass spectrometry."
International Journal of Mass Spectrometry 348: 15-22.
Liang, Z., T. Sham, G. Yang, L. Yi, H. Chen and Z. Zhao (2013). "Profiling of
secondary metabolites in tissues from Rheum palmatum L. using laser
microdissection and liquid chromatography mass spectrometry." Anal Bioanal
Chem 405(12): 4199-4212.
Miquel, M., G. Trigui, S. d'Andrea, Z. Kelemen, S. Baud, A. Berger, C.
Deruyffelaere, A. Trubuil, L. Lepiniec and B. Dubreucq (2014). "Specialization of
oleosins in oil body dynamics during seed development in Arabidopsis seeds."
Plant Physiol 164(4): 1866-1878.
Peukert, M., J. Thiel, D. Peshev, W. Weschke, W. Van den Ende, H. P. Mock and A.
Matros (2014). "Spatio-temporal dynamics of fructan metabolism in developing
barley grains." Plant Cell 26(9): 3728-3744.
Schad, M., R. Mungur, O. Fiehn and J. Kehr (2005). "Metabolic profiling of laser
microdissected vascular bundles of Arabidopsis thaliana." Plant Methods 1(1): 2.
Schiebold, S., H. Tschiersch, L. Borisjuk, N. Heinzel, R. Radchuk and H.
Rolletschek (2011). "A novel procedure for the quantitative analysis of
metabolites, storage products and transcripts of laser microdissected seed
tissues of Brassica napus." Plant Methods 7: 19.
Seaman, C., B. Flinders, G. Eijkel, R. M. Heeren, N. Bricklebank and M. R. Clench
(2014). ""Afterlife experiment": use of MALDI-MS and SIMS imaging for the study
of the nitrogen cycle within plants." Anal Chem 86(20): 10071-10077.
Shroff, R., K. Schramm, V. Jeschke, P. Nemes, A. Vertes, J. Gershenzon and A.
Svatos (2015). "Quantification of plant surface metabolites by matrix-assisted

laser desorption-ionization mass spectrometry imaging: glucosinolates on

Arabidopsis thaliana leaves." Plant J 81(6): 961-972.
Velikovi, D., D. Ropartz, F. Guillon, L. Saulnier and H. Rogniaux (2014). "New
insights into the structural and spatial variability of cell-wall polysaccharides
during wheat grain development, as revealed through MALDI mass spectrometry
imaging." Journal of Experimental Botany 65(8): 2079-2091.
Ye, H., E. Gemperline, M. Venkateshwaran, R. Chen, P. M. Delaux, M. HowesPodoll, J. M. Ane and L. Li (2013). "MALDI mass spectrometry-assisted molecular
imaging of metabolites during nitrogen fixation in the Medicago truncatulaSinorhizobium meliloti symbiosis." Plant J 75(1): 130-145.