Scott Chubb

Mr. Schurtz
English 12 AP Period 3
20 May 2010

Genetic Engineering
 (LA Genetic Engineering)

What is it?
(Dykinga)
 DNA
(DNA)
 (Punnett Square)
(Gregor Mendel)

Mendel and Beyond

(Restriction and Ligation)
(Recombinant Plasmids)
(Transformation of competent…)
Why Should I care?

(Point!)
 Agriculture

(aykırı)
(Zea diploperennis)
 Can You Tell the Difference?

(McIntosh with sticker) (Appleofmyeye)

(Oiled Bird Black)
(Deepwater Horizon fire)
(NOAA Oil Trajectory)
(NOAA Oil Trajectory)
(NOAA Oil Trajectory)
(NOAA Oil Trajectory)
Vibrio parahaemolyticus – try saying that fast

(Vibrio parahaemolyticus)
 Medicine

(Daily Injection)
(Alexander, Jeffrey)
(The Human Genome…)
 Meet ANDi

(Monkey no Glow) (Andi the GM)

(Desert Tree)
(Epidemia de Pánico)
(Twice the Power)
(Oak Knoll Hospital)
(Biohazard Wallpaper)
(Escherichia Coli)
 Glowing
Stuff!

Schurtz’s Picture
(Mad Scientist)
Step 1 – Grow the E.coli
Step 1.5-Pouring Nutrient Plate
Step 2 – E.coli onto plates
Yay! Oops
Transformation
Hmm…That’s not right…
A Short While and a Few Steps Later…
Chromatography: The Final Step
(Hapy Birthday Nikola…)
 Special Thanks to…
• Mr. Werner
• Mr. Manz
• Central Montco Technical High School
•
Works Cited A-G
"Bioremediation." Science Online. Facts on File, n.d. Web. 18 May 2010.
     <http://www.fofweb.com>.
• Bourn, Diane, and John Prescott. A Comparison of the Nutritional Value, Sensory
     Qualities, and Food Safety of Organically and Conventionally Produced
     Foods. N.p.: n.p., n.d. Minnesota Institute for Sustainable
     Agriculture. Web. 17 May 2010. <www.misa.umn.edu/vd/bourn.pdf>.
• "Could color-coded bacteria help spot oil spills?" How Stuff Works. Discovery
     Communications, n.d. Web. 26 Apr. 2010.
     <http://science.howstuffworks.com/color-coded-bacteria.htm>.
• "Custom-Made Monkey Stuns Scientists, Has Ethicists Scratching Heads." Today’s Science On File: n. pag. Today’s Science. Facts On File News Services, 28 Feb. 2001.
Web. 19 May 2010. <http://www.2facts.com/article/s0900940>.
• "Deepwater Horizon Incident." Office of Response and Restoration. NOAA, n.d.
     Web. 18 May 2010. <http://response.restoration.noaa.gov>.
• “Designing the Genes of Future Generations.” Facts on File. 1998. Today’s Science on File. Today’s Science. Web. 1 Dec. 2009. <http://www.2facts.com/_story.aspx?
PIN=s0602800&term=recombinant+dna>.
• Doherty, Tim. "Bacteria could feast on oil in Gulf spill." Hattiesburg American.
     N.p., n.d. Web. 18 May 2010. <http://www.hattiesburgamerican.com/>.
• Ferran, Lee. "Officials Say Burning Oil Rig in Gulf of Mexico Has Sunk." ABC
     World News. ABC, n.d. Web. 18 May 2010. <http://abcnews.go.com>.
• "Fertilizer Helps Microorganisms Clean Pollution." Today's Science. Facts on
     File, n.d. Web. 18 May 2010. <http://www.2facts.com>.
• Galloway, Evan. “Human Genetics: Promise and Controversy.” Facts on File. 2009. Facts On File News Services. Today’s Science. Web. 1 Dec. 2009.
<http://www.2facts.com/_story.aspx?PIN=s1700153>.
• “Gene Therapy - An Overview.” Access Excellence. National Health Museum, 2 Nov. 2009. Web. 1 Dec. 2009.
<http://www.accessexcellence.org////_Therapy_Overview.php>.
• “Genetic Engineering.” Facts on File. Facts on File News Services. Today’s Science. Web. 1 Dec. 2009. <http://www.2facts.com/ _story.aspx?
PIN=xge026200a&term=recombinant+dna>.
• "Genetic Engineering." How Stuff Works. Discovery Communications, 2010. Web. 2
    Mar. 2010. <http://science.howstuffworks.com/
    genetic-engineering-info.htm>.
• Green Fluorescent Protein (GFP) Purification Kit. N.p.: Bio Rad, n.d. Print.
 Works Cited H-Z
• Human Genome Project Information. U.S. Department of Energy Office of Science, Office of Biological and Environmental Research, Human Genome Program, 12 Aug.
2009. Web. 1 Dec. 2009. <http://www.ornl.gov///_Genome/‌home.shtml>.
• "Organic foods: Are they safer? More nutritious?" Mayoclinic.com. Mayo Clinic,
     n.d. Web. 18 May 2010. <http://www.mayoclinic.com/health/organic-food/
     NU00255>.
• Poirot, Lissa. "Genetic Disorders and Engineering: Implications for Physical
   Therapists." Consumer Health Complete. Vol. 13. 2005. 54-60. Magazine of
   Physical Therapy. Consumer Health Complete. Web. 2 Mar. 2010.
   <http://web.ebscohost.com/chc/
   detail?vid=1&hid=5&sid=2e280148-e91b-42a9-baee-a0f15f4dfa58%40sessionmgr12&bdata=
   JnNpdGU9Y2hjLWxpdmU%3d#db=cmh&AN=15933563#db=cmh&AN=15933563#db=cmh&AN=15933563>.
• PGLO Bacterial Transformation Kit. N.p.: Bio Rad, n.d. Print.
• Rhee, Seung Yon. "Gregor Mendel (1822-1884)." Access Excellence. National Health
   Museum, n.d. Web. 2 Mar. 2010. <http://www.accessexcellence.org/RC/AB/BC/
   Gregor_Mendel.php>.
• Rubenstein, Irwin. "DNA." World Book Student. N.p., n.d. Web. 3 Mar. 2010.
     <http://www.worldbookonline.com/student/
     article?id=ar161880&gr=Welcome+Upper+Merion+Area+School+Dist>. "Say No to Genetic Engineering." Greenpeace. N.p., n.d. Web. 2 Mar. 2010.
• "To Build a Better Bandage." Today’s Science On File: n. pag. Today’s Science. Facts On File News Services, 30 Sept. 1999. Web. 19 May 2010.
<http://www.2facts.com/article/s0800370>.
• "What is Genetic Engineering?" Mothers for Natural Law. N.p., n.d. Web. 2 Mar.
   2010. <http://www.safe-food.org/-issue/ge.html>.
 Works Cited Pictures A-G
• Alexander, Jeffrey. iraq. 11 Sept. 2007. Flickr. Yahoo, n.d. Web. 19 May 2010.
     <http://www.flickr.com/photos/35703177@N00/1360676952>.
• Appleofmyeye. 25 Dec. 2007. Flickr. Yahoo, n.d. Web. 17 May 2010.
     <http://www.flickr.com/photos/48718829@N00/2137768494>.
• ANDi the GM monkey. 11 Jan. 2001. Guardian Unlimited. N.p., n.d. Web. 19 May
     2010. <http://www.guardian.co.uk/pictures/image/
     0,8543,-10404115995,00.html>.
• aykırı. 16 Aug. 2007. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/43909439@N00/1135662941>.
• Biohazard Wallpaper. 13 Jan. 2010. Flickr. Yahoo, n.d. Web. 17 May 2010.
• Daily injection. 22 Aug. 2009. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/96526303@N00/3846819118>.
• Deepwater Horizon fire. 21 Apr. 2010. Wikimedia Commons. N.p., n.d. Web. 17 May
     2010. <http://commons.wikimedia.org/wiki/
     File:Deepwater_Horizon_fire_2010-04-21.jpg>.
• Desert Tree. 2 Apr. 2008. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/57075912@N00/2382342057>.
• DNA. N.d. Photobucket. N.p., n.d. Web. 3 Mar. 2010. <http://media.photobucket.com/‌image/‌dna/‌ethamin/‌DNA-colored.gif?o=6&sortby=sevendaysview>.
• Dykinga, Jack. Genetic Engineering Wheat. N.d. Wikimedia Commons. N.p., 2 Jan. 2008. Web. 3 Mar. 2010. <http://commons.wikimedia.org/‌wiki/‌File:Genetic-engineering-
wheat.jpg>.
• Epidemia de Pánico. 24 Apr. 2009. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/99479626@N00/3471986083>.
• Escherichia Coli. N.d. Wikimedia Commons. N.p., n.d. Web. 26 Apr. 2010.
     <http://commons.wikimedia.org/wiki/File:EscherichiaColi_NIAID.jpg>.
• Gregor Mendel. 1928. The History of Biology de Erik Nordenskiöld. Wikimedia Commons. Web. 3 Mar. 2010. <http://commons.wikimedia.org/‌wiki/‌File:Mendel_Gregor_1822-
1884.jpg>.
 Works Cited Pictures H-Z
• Hapy Birthday Nikola Tesla. 10 July 2009. Flickr. Yahoo, n.d. Web. 18 May 2010.
     <http://www.flickr.com/photos/29638083@N00/3707028783>.
• The Human Genome 3. N.d. Flickr. Yahoo, n.d. Web. 3 Mar. 2010. <http://www.flickr.com/‌photos/‌11663483@N00/‌3639793056/>.
• LA Genetic Engineering. N.d. Photobucket. N.p., n.d. Web. 3 Mar. 2010. <http://media.photobucket.com/‌image/‌genetic%20engineering/‌oproomla/OnlyinLA.jpg?o=10>.
• Mad Scientist. N.d. Wikimedia Commons. N.p., n.d. Web. 26 Apr. 2010.
     <http://commons.wikimedia.org/wiki/File:Mad_scientist_caricature_2.png>.
• McIntosh with sticker. 30 Mar. 2006. Wikimedia Commons. N.p., n.d. Web. 17 May
     2010. <http://commons.wikimedia.org/wiki/
     File:McIntosh_with_sticker_by_Lars_Zapf_2006-03-30_cropped.jpg>.
• Monkey no Glow. N.d. Science Magazine. N.p., n.d. Web. 19 May 2010.
     <http://cmbi.bjmu.edu.cn/news/0101/19.htm>.
• NOAA Oil Trajectory. May 2009. Office of Response and Restoration. National
     Oceanic and Atmospheric Administration, n.d. Web. 19 May 2010.
     <http://response.restoration.noaa.gov/
     topic_subtopic_entry.php?RECORD_KEY%28entry_subtopic_topic%29=entry_id,subtopic_i
     d,topic_id&entry_id%28entry_subtopic_topic%29=809&subtopic_id%28entry_subtopic_to
     pic%29=2&topic_id%28entry_subtopic_topic%29=1>.
• Oak Knoll Hospital on LF. 30 Oct. 2007. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/25078342@N00/1806938831>.
• Oiled Bird - Black Sea Oil Spill 11/12/07. 16 Nov. 2007. Flickr. Yahoo, n.d.
     Web. 26 Apr. 2010. <http://www.flickr.com/photos/19378856@N04/
     2037098785>.
• Point! N.d. Flickr. Yahoo, 27 Sept. 2007. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/78453620@N00/1448178195>.
• Punnett Square. 5 May 2007. Wikimedia Commons. N.p., n.d. Web. 3 Mar. 2010. <http://commons.wikimedia.org/‌wiki/File:Punnett_square_mendel_flowers.svg>.
• Recombinant Plasmids. N.d. Flickr. Yahoo, 27 Oct. 2008. Web. 3 Mar. 2010. <http://www.flickr.com/‌photos/‌biotechnology/‌2978906152/‌in/‌set-72157608418301392/>.
• Restriction and Ligation. N.d. Flickr. Yahoo, 27 Oct. 2008. Web. 3 Mar. 2010. <http://www.flickr.com/‌photos/‌biotechnology/‌2978049113/‌in/‌set-72157608418301392/>.
• Transformation of competent E.coli. N.d. Flickr. Yahoo, n.d. Web. 3 Mar. 2010. <http://www.flickr.com/‌photos/‌31836624@N04/‌2978049157/>
• Twice the Power. 3 Mar. 2008. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/21745851@N00/2306555723>.
• Vibrio parahaemolyticus. 2005. Wikimedia Commons. N.p., n.d. Web.
     17 May 2010. <http://commons.wikimedia.org/wiki/
     File:Vibrio_parahaemolyticus_01.jpg>.
• Zea diploperennis. 29 Oct. 2009. Flickr. Yahoo, n.d. Web. 26 Apr. 2010.
     <http://www.flickr.com/photos/35478170@N08/4056260630>.