Serum-Free, Methionine-Free

Insect Cell Culture Medium,

supports excellent [35S]-methionine labeling
efficiencies.
Description
A llele’s Serum-Free, Methionine-Free Insect Culture Box 1 | Basic Info
Medium is specifically developed for the Baculovirus
Expression Vector System (BEVS) technology. It supports Cat. No. ABP-MED-10003
excellent [35S]-methionine labeling efficiencies in Sf9, Sf21, Contents
T.ni, High Five™, and other insect cells.
1 liter of 1X sterile liquid

Storage
Store at +2°C to +8°C, dark. Stable for 2 years if
Usage Details stored properly.

♦ Contains 50 µg/ml gentamycin.

♦ Optimum growth temperature is 27 ± 1°C

♦ Optimum pH is 6.1-6.2 Box 2 | Specifications

♦ Most insect cell lines can be transferred directly into Test Method Specification
the Serum-Free, Methionine-Free Insect Culture Medium
without adaptation. Seeding densities should be 4 x 105 - 1 pH Potentiometric 6.1-6.2
x 106 viable cells per milliliter of culture.
Appearance Visual Clear Yellow
♦ Incubate cells for 30 minutes before adding [35S]- Inspection Solution
methionine. Minimum labeling time should be 1 hour.
Sterility Current USP No Growth
membrane
filtration

F or Research Use Only. Not for

Diagnostic or Therapeutic Use.
Purchase does not include or carry any right
to resell or transfer this product either as a Website: www.allelebiotech.com
stand-alone product or as a component of
Call: 1-800-991-RNAi/858-587-6645
another product. Any use of this product other
than the permitted use without the express (Pacific Time: 9:00AM~5:00PM)
written authorization of Allele Biotech is strictly Email: oligo@allelebiotech.com
prohibited
For Technical Support:
Email:oligo@allelebiotech.com

Related Products
 Sapphire™ Baculovirus DNA  Sapphire™ Insect Transfection Kit
 High level expression. The p10 promoter is  Ideal for easy and efficient transfection of DNAs
partially deactivated and the lytic p10 gene is deleted into insect cells.
so that transcription levels are higher due to reduced
interference and healthier insect cells.
 Serum Free Insect Culture Medium
 Sf9,Sf21, and T.ni Insect Cell Lines  Specifically developed for the Baculovirus
 Commonly used to isolate and propagate Expression Vector System (BEVS) technology.
recombinant baculoviral stocks and to produce
recombinant proteins.

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