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INTRODUCTION
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Chromium (VI) is known to be a strong oxidizing agent, which
apparently poses a high risk yet negative impact to humans and animals
due to its carcinogenetic properties. That is why there are a lots of studies
that have been done profusely in order to determine the health-risky
chromium in environmental as well as biological samples.
AIMS / OBJECTIVES
THEORY
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streams which is nearer a typical congested with automobiles areas are
the most susceptible areas to be contaminated by such chromium.
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standard solution. To calculate the dilute solutions volume, the solution
dilution formula can be used, as what is written below:
(M 1 ) (V 1 ) = (M 2 ) (V 2 ) ....................(1)
A = ɛLC
where
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APPARATUS / REAGENTS
- Distilled water
- spectrophotometer
- 10 millilitres square cuvette bottle
- pipette
- 5 set of 10 millilitres of 1, 20, 60, 85 and 100 p.p.m of diluted
solution of Chromium (VI)
- 5 set of 300 p.p.m of standard Chromium (VI) solution of 0.03, 0.67,
2.00, 2.83 and 3.33 millilitres.
- lake water sample.
EXPERIMENTAL PROCEDURE
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4. 10 millilitres of each series of diluted solution is poured into square
cuvette bottle and placed on the spectrophotometer slot to check the
absorbance value by using the spectrophotometer.
7. All the value is analyzed; the observation and the data obtained are
recorded.
RESULTS
Volume of Chromium
(VI) , millilitres 0.03 0.67 2.00 2.83 3.33
Concentration of
Chromium (VI), parts 1 20 60 85 100
per million
Absorbance Value 0.008 0.131 0.342 0.498 0.594
Table 1.1 Determination of absorbance value using spectrophotometer
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0.65
0.6 0.59
0.55 R² = 1
0.5 0.5
0.45
0.4
0.35 0.34
0.3
0.25
0.2
0.15
0.13
0.1
0.05
0 0.01
0 20 40 60 80 100 120
SAMPLE CALCULATIONS
(M 1 ) (V 1 ) = (M 2 ) (V 2 ).................... (1)
V 1 = 0.03 mL
All the volumes of chromium (VI) solution needed to dilute each standard
solution are stated in Table 1.1.
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By interpolating Graph 1.1, the equation of the linear line is given as;
y = 0.005 x + 0.004
x = y – 0.004
0.005
= 0.253 – 0.004
0.005
= 49.8
DISCUSSION
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The lake water sample used to test for presence of chromium is
believed to be contaminated as the lake is situated near a roadway where
road users with automobiles use it every day. As mentioned before, rivers,
lakes and any water streams which are near to congested areas have the
highest risk or most susceptible to contain heavy metals such as
chromium itself and is then classified as contaminated water source.
Besides that, wrong way of holding the square cuvette bottle used to
contain the diluted solutions might also lead to errors. Any fingerprints or
smudge imprinted on the outer surface of the cuvette can become an
unfavourably interference for the solutions to absorb wavelengths of light
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in the spectrophotometer. Thus, the reading of absorbance by the
instruments may not be accurate.
CONCLUSION
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RECOMMENDATIONS
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REFERENCES
2. 13. Mohamed AA, Ahmed SA, El-Shahat MF. (2001) Catalytic spectrop-
hotometric determination of hexavalent chromium. J. Trace Microprobe
Tech. 19 (2), 297-311.
3. www.googlebooks.com/experimentingeneralchemistryfrommeasurenet
RECOMMENDATIONS
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