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THE AXON GUIDE

A Guide to Electrophysiology & Biophysics Laboratory Techniques

THE AXON GUIDE A Guide to Electrophysiology & Biophysics Laboratory Techniques The Axon Guide — 2500-0102

The Axon Guide — 2500-0102 Rev. C

THE AXON GUIDE A Guide to Electrophysiology & Biophysics Laboratory Techniques The Axon Guide — 2500-0102

Molecular Devices (now part of MDS Analytical Technologies) The Axon Guide A Guide to Electrophysiology & Biophysics Laboratory Techniques

Copyright

Third Edition © Copyright 2008, MDS Analytical Technologies. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language or computer language, in any form or by any means, electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without the prior written permission of MDS Analytical Technologies, 1311 Orleans Drive, Sunnyvale, California, 94089, United States of America.

Trademarks

CYBERAMP, DIGIDATA, GENECLAMP and PCLAMP are registered trademarks and AXOPATCH, AXOCLAMP and MULTICLAMP are trademarks of MDS Analytical Technologies.

These trademarks are not to be used in any type of promotion or advertising without written permission from MDS Analytical Technologies.

All other trademarks or registered trademarks are the property of their respective owners.

Disclaimer

MDS Analytical Technologies reserves the right to change its products and services at any time to incorporate technological developments. This publication is subject to change without notice. Although this manual has been prepared with every precaution to ensure accuracy, MDS Analytical Technologies assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information.

Questions?

Phone: +1-800-635-5577

 

+1-408-747-1700

Fax:

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Web:

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For additional offices, please see the contact information on the back cover of this publication.

2

The Axon Guide — 2500-0102 Rev. C

Contents

List

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.xiii

Preface

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xv

Introduction

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xvii

Editorial

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Contributors

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1. Bioelectricity

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Resistors and Conductors

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The Voltage Divider

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Perfect and Real Electrical Instruments

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Ions in Solutions and Electrodes

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Capacitors and their Electrical Fields

 

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Currents Through Capacitors

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Glass Microelectrodes and Tight Seals

 

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Further Reading

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17

2. The Laboratory Setup

 

The In Vitro Extracellular Recording Setup

 

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The Single-Channel Patch Clamping

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Vibration Isolation Methods

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20

Electrical Isolation Methods

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Radiative Electrical

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Magnetically-Induced

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Ground-Loop Equipment Placement .

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List of Equipment

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Further Reading

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26

The Axon Guide — 2500-0102 Rev. C

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3. Instrumentation for Measuring Bioeletric Signals from Cells

Extracellular Recording

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Single-Cell Recording Multiple-Cell Recording

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Voltage Follower

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Bridge Balance

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Junction Potentials.

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Track

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. Headstage Current Gain

Current Monitor

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33

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Capacitance Compensation

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Leakage Current.

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Headstages for Ion-Sensitive

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Cell Penetration: Mechanical Vibration, Buzz and Clear

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Command Generation

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Large Cells—Two-Electrode Voltage Clamp

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Small Cells—Discontinuous Single-Electrode Voltage Clamp

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Discontinuous Current Clamp (DCC)

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Pipette-Capacitance Compensation Whole-Cell Capacitance Compensation Rupturing the

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Which One Should You Use: dSEVC or cSEVC?

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Space Clamp Considerations

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Single-Channel Patch Clamp

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Resistor Feedback Technology

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Capacitor Feedback Technology

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Special Considerations for Bilayer Experiments

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. Measurement of Changes in Membrane

How Fast is “Fast”?

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Seal and Pipette Resistance Measurement

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Micropipette Holders

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79

80

ii

The Axon Guide — 2500-0102 Rev. C

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80

Whole-Cell Voltage and Current Clamp

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