Alleleustrious pmWasabi-Golgi

Catalog Number: Introduction All ustrious pmWasabi-Golgi is a mammalian expression vector that expresses mWasabi fused to an N-terminal human GALT1 Golgi signal. This product can be a great tool for your studies involving Golgi body localization. GALT1 is also known as B4GALT1, or UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase. All ustrious mWasabi is a monomeric green fluorescent protein that can be easily detected using standard GFP filter sets. mWasabi may be used as a direct replacement for EGFP or other GFPs for superior performance, and may be co-imaged with blue and red fluorescent labels without substantial bleed-through. There are seven human beta-1, 4-galactosyltransferase genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1, 4 linkage to acceptor sugars: GlcNAc, Glc, and Xyl. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. The Golgi apparatus is an organelle found in typical eukaryotic cells. It is responsible for process and packaging of proteins and lipids.
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ABP-FP-WGOL

Source Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Recommended Use mWasabi has been optimized for use with standard GFP/FITC filter sets. Features About 2-fold brighter than EGFP Similar photostability to EGFP Uses standard filter sets Can be co-imaged with blue and red FPs or dyes Mammalian expression vector ready to transfect your favorite cells Low sensitivity to acidic pH (fluorescence pKa=4.3) True monomer that will not aggregate or cause nonspecific interactions

Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Golgi targeting signal.597-842 Linker......843-863 Wasabi........................................864-1574 SV40 Poly A Signal....................1726-1776 bla Promoter...............................2315-2419 Ampicillin Resistance Gene......2404-3264 pUC Origin..................................3413-4055 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A AMPR pUC Ori Golgi Targeting Signal PCMV

pmWasabiGolgi 4137bp

Linker

Wasabi

References: Ai H, Olenych SG, Wong P, Davidson MW, Campbell RE. Hue-shifted monomeric variants Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biology. 2008 Mar; 6:13. Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology. J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Schaub,B.E., Berger,B., Berger,E.G. and Rohrer,J. (2006). "Transition of galactosyltransferase 1 from trans-Golgi cisterna to the trans-Golgi network is signal mediated" Mol. Biol. Cell 17 (12), 5153-5162Li,Z., Zong,H., Kong,X., Zhang,S., Wang,H., Sun,Q. and Gu,J. (2006). Cell surface beta 1, 4-galactosyltransferase 1 promotes apoptosis by inhibiting epidermal growth factor receptor pathway Mol. Cell. Biochem. 291 (1-2), 69-76

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Golgi
SEQUENCE
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG GGCTTCGGGA CCTGCCGCCT TGGCTGGCCG GCGGCTCGAA CCAAGGATCC TCAAGCCCGA TGATCGAGGG TGAAGGAGGG GCAACAGGGC CCGAGGGCTA AGTCCGACAT ACTTCCCCCC AGAGGATGTA AGGGCGGCGG AGCTGCCCGA ACAACAAGGT AGCTGTACAA GTTTTACTTG GCAATTGTTG ATCACAAATT CTCATCAATG TACGCGCAGC CCCTTCCTTT TTTAGGGTTC TGGTTCACGT CACGTTCTTT CTATTCTTTT GATTTAACAA ACTTTTCGGG ATGTATCCGC AGTATGAGTA CCTGTTTTTG GCACGAGTGG CCCGAAGAAC TCCCGTATTG TTGGTTGAGT TTATGCAGTG ATCGGAGGAC CTTGATCGTT ATGCCTGTAG GCTTCCCGGC CGCTCGGCCC TCTCGCGGTA TACACGACGG GCCTCACTGA GATTTAAAAC ATGACCAAAA ATCAAAGGAT AAACCACCGC AAGGTAACTG TTAGGCCACC TTACCAGTGG TAGTTACCGG TTGGAGCGAA ACGCTTCCCG GAGCGCACGA CGCCACCTCT AAAAACGCCA ATGTTCTTTC TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA GCCGCTCCTG GCTCGTGGCC CGACCTGAGC CAGTGCCGCC ACCGGTCGCC CATGAAGATC CGAGGGCGAG AGCCCCCCTG CTTCACCAAG CTCTTGGGAG CTCCATGGAG CAACGGCCCC CGTGCGCGAC CCACCACCGC CTATCACTTT GACCGTTTAC GTAAGCGGCC CTTTAAAAAA TTGTTAACTT TCACAAATAA TATCTTAACG GTGACCGCTA CTCGCCACGT CGATTTAGTG AGTGGGCCAT AATAGTGGAC GATTTATAAG AAATTTAACG GAAATGTGCG TCATGAGACA TTCAACATTT CTCACCCAGA GTTACATCGA GTTTTCCAAT ACGCCGGGCA ACTCACCAGT CTGCCATAAC CGAAGGAGCT GGGAACCGGA CAATGGCAAC AACAATTAAT TTCCGGCTGG TCATTGCAGC GGAGTCAGGC TTAAGCATTG TTCATTTTTA TCCCTTAACG CTTCTTGAGA TACCAGCGGT GCTTCAGCAG ACTTCAAGAA CTGCTGCCAG ATAAGGCGCA CGACCTACAC AAGGGAGAAA GGGAGCTTCC GACTTGAGCG GCAACGCGGC CTGCGTTATC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG AGCGGCAGCG GTCTGCGCTC CGCCTGCCCC GCCATCGGGC ACCATGGTGA AAGCTGAAGA GGCAAGCCCT CCCTTCTCCT TACCCCGACG CGCACCATGA GAGGACTCCT GTGATGCAGA GGCGTGCTGA GTTGACTTCA GTGGACCACC GAGAGCGCCG GCGACTCTAG CCTCCCACAC GTTTATTGCA AGCATTTTTT CGTCCTGTAG CACTTGCCAG TCGCCGGCTT CTTTACGGCA CGCCCTGATA TCTTGTTCCA GGATTTTGCC CGAATTTTAA CGGAACCCCT ATAACCCTGA CCGTGTCGCC AACGCTGGTG ACTGGATCTC GATGAGCACT AGAGCAACTC CACAGAAAAG CATGAGTGAT AACCGCTTTT GCTGAATGAA AACGTTGCGC AGACTGGATG CTGGTTTATT ACTGGGGCCA AACTATGGAT GTAACTGTCA ATTTAAAAGG TGAGTTTTCG TCCTTTTTTT GGTTTGTTTG AGCGCAGATA CTCTGTAGCA TGGCGATAAG GCGGTCGGGC CGAACTGAGA GGCGGACAGG AGGGGGAAAC TCGATTTTTG CTTTTTACGG CCCTGATTCT ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC CCGCGATGCC TGCACCTTGG AACTGGTCGG AGTCCTCCGG GCAAGGGCGA TGGAGGGCAA ACGACGGCAC ACGACATTCT ACATCCCCAA CCTTCGAGGA TCATCTACGA AGAAGACCAC AGGGCGACGT AGACCATCTA GCATCGAGAT TGGCCCGCAA ATCATAATCA CTCCCCCTGA GCTTATAATG TCACTGCATT CGGCGCATTA CGCCCTAGCG TCCCCGTCAA CCTCGACCCC GACGGTTTTT AACTGGAACA GATTTCGGCC CAAAATATTA ATTTGTTTAT TAAATGCTTC CTTATTCCCT AAAGTAAAAG AACAGCGGTA TTTAAAGTTC GGTCGCCGCA CATCTTACGG AACACTGCGG TTGCACAACA GCCATACCAA AAACTATTAA GAGGCGGATA GCTGATAAAT GATGGTAAGC GAACGAAATA GACCAAGTTT ATCTAGGTGA TTCCACTGAG CTGCGCGTAA CCGGATCAAG CCAAATACTG CCGCCTACAT TCGTGTCTTA TGAACGGGGG TACCTACAGC TATCCGGTAA GCCTGGTATC TGATGCTCGT TTCCTGGCCT GTGGATAACC AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC AGGCGCGTCC CGTCACCCTC AGTCTCCACA GGAGCTCCGG GGAGACCACA CGTGAATGGC CAACACCATC GACCACCGCG CTACTTCAAG CAAGGGCATC GATACACCTC CGGCTGGGAC CAAGCACAAG CAGGGCCAAG CCTGAACCAC CTCCACCGAC GCCATACCAC ACCTGAAACA GTTACAAATA CTAGTTGTGG AGCGCGGCGG CCCGCTCCTT GCTCTAAATC AAAAAACTTG CGCCCTTTGA ACACTCAACC TATTGGTTAA ACGCTTACAA TTTTCTAAAT AATAATATTG TTTTTGCGGC ATGCTGAAGA AGATCCTTGA TGCTATGTGG TACACTATTC ATGGCATGAC CCAACTTACT TGGGGGATCA ACGACGAGCG CTGGCGAACT AAGTTGCAGG CTGGAGCCGG CCTCCCGTAT GACAGATCGC ACTCATATAT AGATCCTTTT CGTCAGACCC TCTGCTGCTT AGCTACCAAC TTCTTCTAGT ACCTCGCTCT CCGGGTTGGA GTTCGTGCAC GTGAGCTATG GCGGCAGGGT TTTATAGTCC CAGGGGGGCG TTTGCTGGCC GTATTACCGC TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCATGA CTACAGCGGG GTTTACTACC CCGCTGCAGG ACCGGAGGGG ATGGGCGTAA CACGCCTTCG AACCTGGAGG TTCGCCTACG CAGTCCTTCC GTGAAGGTGA AAGGGCGAGA GCCTCCACCG CTGCTGCTGG AAGGCGGTGA GACAAGGACT GGCATGGACG ATTTGTAGAG TAAAATGAAT AAGCAATAGC TTTGTCCAAA GTGTGGTGGT TCGCTTTCTT GGGGGCTCCC ATTAGGGTGA CGTTGGAGTC CTATCTCGGT AAAATGAGCT TTTAGGTGGC ACATTCAAAT AAAAAGGAAG ATTTTGCCTT TCAGTTGGGT GAGTTTTCGC CGCGGTATTA TCAGAATGAC AGTAAGAGAA TCTGACAACG TGTAACTCGC TGACACCACG ACTTACTCTA ACCACTTCTG TGAGCGTGGG CGTAGTTATC TGAGATAGGT ACTTTAGATT TGATAATCTC CGTAGAAAAG GCAAACAAAA TCTTTTTCCG GTAGCCGTAG GCTAATCCTG CTCAAGACGA ACAGCCCAGC AGAAAGCGCC CGGAACAGGA TGTCGGGTTT GAGCCTATGG TTTTGCTCAC CATGCAT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Golgi
Enzyme AatII Acc113I Acc16I AccB1I AccB7I AccBSI AccIII AcsI AcyI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I ApaLI ApoI AseI AsnI Asp700I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbsI Bbv12I Bbv16II BcgI BcoI BglI BpiI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseAI BseRI BsgI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp13I Bsp1407I Bsp143II Bsp19I BspCI BspEI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI BstZI CciNI Cfr10I CfrI CpoI CspI DraI Cuts 5 1 1 4 1 4 1 3 8 2 1 1 4 2 1 1 2 3 2 2 1 1 4 1 1 1 1 4 3 8 1 4 1 1 1 5 1 3 1 3 1 8 2 2 5 5 4 1 1 2 1 5 1 4 5 4 3 1 2 1 1 1 5 3 1 1 2 1 4 1 2 4 1 4 3 5 4 4 1 5 1 5 1 7 1 9 1 9 1 1 4 6 1 1 4 Positions 125 178 261 447 1360 2710 2968 465 996 2007 3238 1303 617 625 1907 2352 826 1746 2231 2242 122 175 258 444 700 1309 1357 2651 1818 4079 851 3191 826 2523 2608 3769 2519 3765 3670 1140 2519 3765 1746 2231 2242 7 3016 7 3016 2591 3191 826 2523 2608 3769 1140 2968 949 845 465 996 2007 3238 826 1034 1977 122 175 258 444 700 1309 1357 2651 1297 826 2523 2608 3769 1297 2675 1140 90 212 283 1431 3073 1297 1019 1382 3106 1297 340 1331 2048 1594 122 175 258 444 700 1309 1357 2651 887 3124 1682 1781 856 1579 2673 2822 3745 826 851 2895 3726 3873 851 1299 1943 3106 1594 826 884 1223 695 856 1579 2673 2822 3745 1594 465 996 2007 3238 856 1579 2673 2822 3745 826 2523 2608 3769 677 2527 3911 826 1682 1781 1140 826 1564 631 1528 1893 1901 3839 360 862 1213 2822 826 2351 3359 4079 617 625 1907 2352 1594 2955 3137 851 1299 1943 3106 1564 851 1299 1943 3106 677 2527 3911 360 695 841 862 1213 617 625 1907 2352 360 862 1213 1528 1508 631 1528 1893 1901 3839 845 856 1579 2673 2822 3745 1508 651 774 1418 1825 2945 3623 3814 340 585 845 1477 2544 2561 3329 3341 3427 3438 1013 585 845 1477 2544 2561 3329 3341 3427 3438 1576 1576 851 1299 1943 3106 676 725 947 1388 1576 2798 829 829 1634 2613 3305 3324 Sequence gacgt/c agt/act tgc/gca g/gyrcc ccannnn/ntgg gagcgg t/ccgga r/aatty gr/cgyc a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg g/tgcac r/aatty at/taat at/taat gaann/nnttc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gaagac gwgcw/c gaagac cgannnnnntgc c/ycgrg gccnnnn/nggc gaagac ctggag gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc t/ccgga gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg t/ccgga t/gtaca rgcgc/y c/catgg cgat/cg t/ccgga t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy c/ggccg gc/ggccgc r/ccggy y/ggccr cg/gwccg cg/gwccg ttt/aaa DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco24I Eco255I Eco31I Eco52I Eco57I Eco64I Eco88I Eco91I EcoICRI EcoO109I EcoO65I EcoT14I EcoT22I ErhI Esp1396I FauNDI FriOI FspI GsuI HaeII Hin1I HincII HindII HpaI Hsp92I Kpn2I Ksp632I MamI MfeI MflI MluI MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI PflMI PinAI Ple19I Ppu10I PshBI Psp124BI Psp1406I PspEI PstI PstNHI PvuI RcaI RsrII SacI ScaI SfcI SnaBI SspBI SspI SstI StyI Van91I VneI VspI XbaI XcmI XhoII XmaIII XmnI Zsp2I 1 2 2 4 6 1 1 1 1 1 1 1 1 5 3 1 2 1 4 4 1 1 1 1 1 5 1 5 1 1 3 1 3 5 8 2 2 1 8 1 1 1 1 9 1 1 1 1 1 1 6 8 5 1 2 1 3 1 1 1 1 1 1 5 1 1 1 1 1 2 1 2 1 1 1 1 2 1 1 1 7 1 1 2 1 5 1 2 2 1 1 9 1 1 1 1087 1460 2051 2095 3977 360 862 1213 1528 676 725 947 1388 1576 2798 1576 2402 3191 2402 824 3191 1576 340 360 695 841 862 1213 826 1034 1977 2710 887 3124 1576 929 1352 2509 3557 465 996 2007 3238 1140 1508 824 1087 1508 360 695 841 862 1213 4136 360 695 841 862 1213 1303 234 826 1034 1977 2968 1019 1382 3106 631 1528 1893 1901 3839 122 175 258 444 700 1309 1357 2651 1403 1695 1403 1695 1695 122 175 258 444 700 1309 1357 2651 826 2402 1594 1682 585 845 1477 2544 2561 3329 3341 3427 3438 1818 949 4136 826 1943 949 365 864 1212 2420 2779 2938 122 175 258 444 700 1309 1357 2651 656 773 2555 3496 3741 1682 1682 1781 1945 360 862 1213 234 1943 1943 591 1576 4136 656 773 2555 3496 3741 4083 1303 851 2822 4132 7 3016 826 2589 2962 1508 778 591 2822 2351 3359 829 826 2710 651 774 1418 1825 2945 3623 3814 340 1564 2256 2386 826 360 695 841 862 1213 1303 2519 3765 7 3016 1586 1550 585 845 1477 2544 2561 3329 3341 3427 3438 1576 2591 4136 rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg grgcy/c agt/act ggtctc c/ggccg ctgaag g/gyrcc c/ycgrg g/gtnacc gag/ctc rg/gnccy g/gtnacc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac gtt/aac gr/cgyc t/ccgga ctcttc gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y ccannnn/ntgg a/ccggt cgat/cg a/tgcat at/taat gagct/c aa/cgtt g/gtnacc ctgca/g g/ctagc cgat/cg t/catga cg/gwccg gagct/c agt/act c/tryag tac/gta t/gtaca aat/att gagct/c c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg r/gatcy c/ggccg gaann/nnttc atgca/t

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmWasabi-Golgi
The following enzymes do not cut: AatI, Acc65I, AccI, AclNI, AfeI, AflII, AocI, Aor51HI, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbuI, BclI, BfrI, BglII, BlnI, BlpI, Bpu1102I, Bpu14I, Bsa29I, BscI, Bse21I, BseCI, BsePI, BsiWI, BsmBI, Bsp106I, Bsp119I, Bsp120I, Bsp1720I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, Bsu15I, Bsu36I, CelII, Cfr42I, Cfr9I, ClaI, Csp45I, CvnI, Eco147I, Eco32I, Eco47III, Eco72I, Eco81I, EcoNI, EcoRI, EcoRV, EheI, Esp3I, FbaI, FseI, HindIII, KasI, KpnI, Ksp22I, KspI, LspI, MspCI, NarI, NruI, NspV, PacI, PaeI, PaeR7I, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp5II, PspAI, PspALI, PspLI, PspOMI, PvuII, SacII, SalI, SapI, SbfI, SexAI, SfiI, Sfr274I, Sfr303I, SfuI, SgfI, SgrAI, SmaI, SmiI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XhoI, XmaI

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010