INSTRUMENTA

L SEMINAR
By
Dr Deepti Patil
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ROTAROD
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Dr
Deepti
4/8/11 ROTAROD
Patil 22
 INTRODUCTION
The loss of muscle grip is an indication
of muscle relaxation.

This effect can be easily studied in

animals using inclined plane or
rotating rod

The fall off time from the rotating rod

between
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control and test
ROTAROD
drug treated 33
 HISTORY
The “Rota-Rod” technique has been
originated by a 1957 paper of N.W
Dunham and T.S Miya and has proved
to be of great value in research
involving screening of drugs which are
potentially active on motor co-
ordination.
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 PRINCIPLE OF OPERATION

When a mouse falls off in its cylinder

section onto the plate below, the plate
trips and the corresponding magnetic
switch is activated, thereby recording
the animal’s endurance (staying
power) time in seconds.

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 Conti…

The display shows the actual angular speed

(RPM). At the end of a run, the display
shows for each animal the running time
and the instrument rotation speed at the
time the animal fell off.

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 MOTOR SKILLS TESTS
Drug-induced turning (Rotation Test)
Forelimb asymmetry (Cylinder Test)
Beam walking
Grip strength
Grid walking
Placing test
Rotarod
Landing Foot Spread Test
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 MOTOR PROBLEMS
Disorders may have both peripheral
and central
origins:
• Cerebellum
• Brain stem
• Striatum
• Basal ganglia
Motor cortex
•4/8/11
Spinal cord ROTAROD 88
 ANIMAL TESTS FOR MOTOR
ABILITY
Balance (e.g. Rotarod)

Reflex testing

Strength testing

Gross activity levels (GAL)

Fine motor analysis (FMA)

Straight observation
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 ROTAROD
Rotarod provides an easy way to test the
motor activity in rodents.
Ø Central nervous system damage,
Ø Disease effects on motor activity,
Ø Drugs administration, and
Ø Fatigue
Assessed by measuring the time during
which the animal will keep on walking on
the rotating bar

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 USES

Motor abnormalities:

• Coordination

• Weakness

• Muscle tonicity

• Involuntary movements

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 CONTI….

Other domain:

• Sensory function

• Cognitive ability

• Anxiety

• Non-motor seizures

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 TYPES
1. Three lane Rotarod

2. Four lane Rotarod

3. Five lane Rotarod

4. Rotarod with increased falling

distance

5. Mice Rotarod
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 PARTS

Cover Set
Timer knobs for operating the
individual lanes
Floor Grids
Droppings collector

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 PROCEDURE
Weigh the animals and number them

Turn on the Rota rod. Select an

appropriate speed (20-25rpm is ideal).

Place the animals one by one on the

rotating rod

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 Conti…
Note down the fall off time when the
mouse falls from the rotating rod

A normal mouse generally falls off

within

3-5min

Compare the fall off time before and

after
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 “Placing the Mice on the
Click toRotor”
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THANK YOU
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