Class dsDNA Polymoaviridae E.g BK and JC, Hendra?

Virion contains Closed circular Genome packed by histones

ORFS and size

Diversity/SPlicing Diff splicing: generates LT and sT (shorter or longer RF) Leaky scanning recognises start/kozak poor consensus willb e skipped. ORFs are partially overlapping

RNApol RNA pol2

DNApol Host requires LT antigen

Temporal control High LT antigen inhibits early expression activates late express.

Poxviridae e.g. Variola

Complete machinery

130-250kb (100200genes)

Viral Na Pol2

Viral

Adenoviridae

Genome

VA-RNA produced by RNA pol 3 interacts with cellular proteins

RNA pol2 and 3

Viral (forms pTP-pol complex) primes synthesis of new strand

Herpesviridae

Genome + VP16 (needs to bind Oct-1)

125-240kb (70200ORFS)

Concatemers are important

RNA pol2

Viral

Early transcription incore (mRNA extruded out) translation by uncaoting E1A E2A IVA2 L1,L2 (immediate early required for early gene expression which is itself required for late Immediate early initiated upon entry of dsDNA into host nucleus. IE (a) E(b) L (g) genes Transcription dependent on E1A (strand displacement mechanism)

ssDNA Parvovridae (B19)

Genome (adeno helper provides E1)

4-6kb (2ORFS)

Alternative splicing (Rep and cap ) proteins

RNA pol2

Host

dsDNA retrovirus Hepadnaviridae Hep B +ssRNA Picornaviridae

Genome + P protein

3.2kb Multiple overlapping ORFS

Multiple transcripts generating different intiation codons Polyprotein Cis and Trans (2Cpro,3Cpro) Infection shuts down cell translation IRES unaffected SUpression of termination multiple proteins from single mRNA.

Rna pol2

Viral P protein + Pregenome

Genome

7-8kb single ORF

3D pol

Togaviridae

Genome

2 ORFS 10-13kb

-ssRNA Orthomyxoviridae

Segmented Genome

6-8ORFS 10-15kb

Seg

Viral

Nucleocapsid protein critical threshold changes transcriptase activity to ssRNA

-ssRNA satellite Hepatits D dsRNA viruses Reoviridae |E.g. Rotavirus +ssRNA retrovirus Retroviridae e.g. HIV

Genome Segmented Genome

1.7kb 11ORFS 19-32kb

Host RNA pol 2 Viral genome and RNA pol activity bound to capsid Ribosomal frameshifting mRNA associate with newly formed capsid and ssRNA made

Genome Pseudo-diploid

7-11kb (4 main genes)