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MODUL JUJ

PROJEK JAWAB UNTUK JAYA (JUJ) NEGERI PAHANG TAHUN 2010

BIOLOGI

Page 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Contents Format Analysis Tips Questions - SPM 2009 Question paper (paper 2 & 3) Marking scheme Example of students answer Practice Module Marking Skill 2 3-4 5 - 10 11 -18 19 - 47 48 - 74 75 -115 116 - 165 165 - 208

1.2
Index 1 2

BIOLOGY SPM EXAM FORMAT ( STARTING FROM 2003) SUBJECT CODE : 4551
Criteria Type of instrument Type of item Paper 1(4551/1) Objective Test Objective Item Multiple choice Each item followed by four alternative answers A, B, C or D 50 ( Answer all the questions ) Paper 2(4551/2) Subjective Test Paper 3(4551/3) Written Practical

Subjective Item : Subjective Item Structure Section A : structured Item Item Open ended Section B :Essay Respond Item (Essay ) Section A : 5 items (Answer all the questions Section B : 4 items (Answer any two questions) Refer to SPM 2008 format (latest) Structure Item 1 2 item (Answer any one question) Open ended responds: 1 item ( Essay written ) 50 Write the answer in the space provided in the question paper 1 hour 30 minutes Science process skill : 16 aspect Max score :3 Refer Example instrument: Paper 4551 / 3

Total Question

4 5

Total Marks Responded

50 Blacken one space at OMR form 1 hour 15 minutes section A : Knowlegment 25 Section B : Understanding 15 Section C : Application skill - 10 Refer Example Instrument: Paper 4551 / 1

100 Write the answer in the space provided in the question paper 2 hours 30 minutes Knowlegment 10 Understanding 20 Application skill 30 Analysis skill 15 Synthesis skill 15 Evaluation - 10 Refer Example instrument: Paper 4551 / 2

6 7

Duration Construct scoring

Item example based on construct

9 10 11

Marking Context Level of difficulties Easy : E Moderate : M Hard : H Adding apparatus

Dichotomous Mark: 1 or 0 Construct from All learning Area are tested R:S:T=3:1:1 ( 25 easy item : 15 moderate item :10 hard item )

Scoring is analytical based on scoring rubric Construct from All learning Area are tested R:S:T=4:4:2 ( 40marks easy Item: 40marks moderate item : 20marks hard item ) Overall R:S:T=5:3:2 Scientific calculator

Scoring is analytical based on rubric at level 3. Construct are tested from suitable learning area. R:S:T=3:1:1 ( 30 easy item : 10 moderate item : 10 hard item )

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Scientific calculator

Scientific calculator

1.3 Analysis of the SPM Biology Exam Questions


Analysis of the SPM Biology Questions (2005-2009)

CHAPTER 1. Introduction of Biology 2. Cell Structure and Cell Organisation 3. Movement of substance Across The Plasma Membrane 4. Chemical Composition Of The Cell 5. Cell Division 6. Nutrition 7. Respiration 8. Dynamic Ecosystem 9. Endangered Ecosystem

2005 P1 P2 P3 OBJ S E 1 2 - - - 4 3 1 1

P1 OBJ 2 4

2006 P2 P3 S E 1 2 - - - 1 1 1

P1 OBJ 4 2

2007 P2 S E 1/2 1 -

P3 1 2 - -

P1 OBJ 2 3

2008 P2 S E 1/5 4/5

P3 P1 1 2 OBJ - 1 2 5

2009 P2 P3 S E 1 2 - 1 1/5 -

F O R M F O U R

3 2 6 2 3 3

1 1 1 1 -

1 -

2 3 4 2 4 2

1 -

2 -

1 -

1 2 9 4 5 3

1 1/3 -

1 1 1/3

1 -

3 -

1 -

1 1

3 2 6 6 3 4

2/3 1 -

1 -

1 -

8 5 3

1 1 1 -

1 -

1 -

CHAPTER F O R M F I V E 1. Transport 2. Locomotion and Support 3. Coordination and Response 4. Reproduction and Growth 5. Inheritance 6. Variation

P1 OBJ 5 3 5 5 4 2

2005 P2 P3 S E 1 2 - - - 1 -

P1 OBJ 5 3 5 9 2 3

2006 P2 P3 S E 1 2 - 1 - 1 1 1 -

P1 OBJ 4 7 6 2 3

2007 P2 S E 2/3 2/3 1 1 1 -

P3 1 2 - -

P1 OBJ 6 3 5 5 2 2

2008 P2 S E 1 1 1 1 -

P3 1 2 - -

P1 OBJ 7 1 4 4 2 1

2009 P2 P3 S E 1 2 1 1 1 1/3 1 -

1 1 -

EXPERIMENTS CHECKLISTS FORM 4 (SPM 2004-2009) No


1 2

Topic
CHAPTER 3: Akt:3.1 Size of molecule that can diffuse through a semipermeable membran CHAPTER 3: Movement of substances across the plasma membrane Activity 3.2 : Studying osmosis using an osmometer (page 24)

2004 Q1 Q2

2005 Q1 Q2

2006 Q1 Q2

2007 Q1 Q2

2008 Q1 Q2 X

2009 Q1 Q2

CHAPTER 3: Movement of substances across the plasma membrane Activity 3.4 and 3.4 : Studying the effects of hypotonic ,hypertonic and isotonic solutions on animal and plant cells. (27-28)

CHAPTER 3: Movement of substances across the plasma membrane Activity 3.6 : Determining the concentration of an external solution which is isotonic to the cell sap of a plant. (page 30)

CHAPTER 4: Chemical composition of the cell Activity 4.3: Studying the effects of temperature on salivary amylase activity (page 36)

CHAPTER 4: Chemical composition of the cell Activity 4.4: Studying the effects of pH on the activity of pepsin (page 39)

CHAPTER 4: Chemical composition of the cell Activity 4.4: Investigate the effects of pH on the breakdown of starch by amylase. (page 41)

CHAPTER 4: Chemical composition of the cell Activity 4.5: Studying the effects of substrate concentration on salivary amylase activity (page 42) /(SPM : Concentration of albumen)

CHAPTER 4: Chemical composition of the cell Activity 4.6: Studying the effects of enzyme concentration on salivary amylase activity (page 43)

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CHAPTER 6: Nutrition Activity 6.1: Determining the energy value in food samples. (page 61 62)

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CHAPTER 6: Nutrition Activity 6.3: Determining the vitamin C contain in various fruit juices. (page 65 66)

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CHAPTER 6: Nutrition Activity 6.8 : Studying the effects of macronutrient deficiency in plants (page 72)

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CHAPTER 6: Nutrition Activity 6.11 Investigating the effects of light intensity on the rate of photosynthesis. (page 76)

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CHAPTER 6: Nutrition Activity 6.11 Investigating the effects of carbon dioxide concentration on the rate of photosynthesis. CHAPTER 7: Respiration Activity 7.6: Investigating the differences between inhaled and exhaled air in terms of oxygen and carbon dioxide contents. (page 93) (page 93 94)

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CHAPTER 7: Respiration Activity 7.2 : Investigating the process of anaerobic respiration in yeast (page 85) CHAPTER 8: Dynamic Ecosystem Activity 8.5 Investigating the distribution of plants using the quadrat sampling technique (page 111- 112) Modified (using Grid) X

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CHAPTER 8: Dynamic Ecosystem Activity 8.6 Estimating the population size of animals using capture, mark, release and recapture technique (page 113) X

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CHAPTER 8: Dynamic Ecosystem Activity 8.11 Studying the effects of temperature, pH, light intensity and nutrients on the activity of yeast (page 119) X

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CHAPTER 9: Endangered Ecosystem Activity 9.2: Investigating the level of pollution in several different sources of water (page 128 129)

EXPERIMENTS CHECKLISTS FORM 5 (SPM 2003-2009) No 1 Topic CHAPTER 1:TRANSPORT To study one of the factor of affecting the rate of transpiration. 2 CHAPTER 3: COORDINATION AND RESPONSE. - To study the effect of different quantities of water intake of urine output. CHAPTER : VARIATION - To investigate continuous variation and discontinuous variation in human. CHAPTER 6: VARIATION - To investigate the importance of camouflage in the survival of a species 2003 2004 2005 2006 2007 2008 2009

Q1

Q2

Q1

Q2

Q1

Q2

Q1

Q2

Q1

Q2

Q1

Q2

Q1

Q2

4.

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1.4 TIPS FOR EXAM


1.4.1 Objective Question Paper1 i. ii. iii. iv. v. Try to answer easy questions first, followed by moderate questions and students have enough time to answer difficult questions. Dont take more than 11/2 minutes for each question to make sure enough time for all questions. Read the question carefully for three times to you understand what are the questions ask. More information for each question can get from graph, table, and diagram that given. Make ( / ) for true statement, reject all destructor and guess the best answer when you are not sure the best answer. Make sure answer all the questions and remark all the answer and make sure: * One question only one answer. * Deleted wrong answer completely * Used 2B pencil. Vii Examples of questions form for paper 1 * Remember the fact * Making conclusion * Application * Observation * Knowlegment * Comparisons * Identify the problem * Calculation

vi.

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1.4.2 Subjective Question

Encourage the students to review the essay question first (Part B Paper2 ),before answer the structure question, this because students will have enough time to think some facts or explaination. Almost structure questions based on diagram, table, data, flow chart, graph that suitable with fact, experiment or investigation. Understand all the information given. Time suggestion to answer Paper 2: Part A ( 90 minutes ), Part B ( 60 minutes ), for Paper 3 : Question 1 ( 50 minutes ) and Question 2 ( 40 minutes ) Answer in one word, one number or one simple sentence Dont combine the right fact with the wrong fact Follow the instruction like : Give two examples of., so students should give only two examples, the third example will not get the mark. No need write in long sentence or copy again part of the question. Answer can be in equations form, diagram, table or graph. Calculation must be show. Space for write the answers and mark at end of the essays or structure questions are given will show how long the answer must be write. Characteristics of alveolus : Accept Thickness of alveolus is only one cell Surface of alveolus is wet A lot of network of blood capillaries covering the alveolus Reject Alveolus is thin wet A lot of blood capillaries

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Instruction verb like justification, evaluation, give your opinion, Students must state like I agree / I accept / Im not agree / Im not accept that statement given ( 1 mark ) and followed by opinion Draw a diagram * No artistic * Big (suitable size), clear, * Label the diagram correctly and line for label cant be cross together * Neat and without broken lines Draw a enzyme structure: Size and shape of the enzyme must same with the original

Comparison - Must have similarities and differences - One characteristic must compare between two subject in one sentence - Separate sentence between similarities and differences - If answer in table, must write in full sentence Write chemical equation : * In word form [ / ] Glucose + oxygen [ X ] Glucose + oxygen * In chemical form [ / ] C6H12O6 + 6O2 6CO2 + 6H2O + energy Carbon dioxide CO2 + H2O + water + energy + energy

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Draw the hybrid cross (Inheritance) * Has key * Label the schema diagram - Parental Genotype - Parental Gamete - F1 Genotype - F1 Phenotype Male gamete and female gamete are fertilization * Reject combine / attach Function of mitochondrion Generate / provide energy - Reject : Supply / give energy Dont copy again part of the question because this is not get any mark.

1.4.3 Paper 3 1.4.3.1 Question 1 i) Measuring using number Measure / record the data using apparatus that given in the experiment / question with the correct unit Example : Record scale / thermometer reading, stop watch, ruler, measuring cylinder, syringe, burette with the correct units ( if not given)

ii) Observing Making observation based on the experiment given not on the theory. What can observe / see only from data, table, scale of apparatus Example : State changes in color State increase of thermometer reading State changes in time State changes in volume ( end of experiment ) State the value correctly from data The observation that can be making inference

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iii) Making Inferences

- Making initial conclusion / cause based on observation - Inferences must be correspond with the observation ( inference (i) correspond with observation (i) , inference (ii) correspond with observation (ii)

If wrong / reject observation automatic inference will reject / wrong iv) Controlling Variables - Able to state all the variables, controlled, responding and manipulated variables correctly and method to handle variable correctly. - Must state PARAMETER like volume, temperature, mass, time, length - State that apparatus using to get the result for responding and controlled variables. Variable Manipulated variable: Variables that are changed in the experiment Examples: Temperature of water bath, mass of food, concentration of sucrose solution, type of fruits Method to handle variable correctly Change in mass/concentration / water Or used different mass/ concentration / type of food Example : Used different mass of food Used 30% sucrose solution, 5% sucrose solution 10% sucrose solution Replace papaya juice with orange juice Change the concentration of albumen Must state the apparatus or state the formula using Example : i) Measure and record the final length of potato strip using ruler ii) Measure and record the final temperature of water using thermometer iii) Calculate the rate of transpiration using formula : distance divided by time iv) Calculate the rate of enzyme reaction using formula concentration of albumen dived by time Must state the PARAMETER and VALUE and APPERATUS

Responding variable: Variable that are measure after experiment / result Example i) Final length of potato strip, ii) Final temperature of water, iii) Rate of transpiration iv) Rate of enzyme reaction

Controlled variable: Variable that constant during experiment Example:

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Initial temperature of water, volume Example : Fix the temperature at 370C using of water, concentration of starch, thermometer type of enzyme Fix volume of water at 20ml using measuring cylinder Fix concentration of starch at 10% Fix type of enzyme is pepsin v) Making hypothesis Make a statement of hypothesis by relating the manipulated variable (MV) with the responding variable (RV) and showing the specific relationship (H). vi) Communication Presenting the data in certain form like table, graph, chart or diagram. Table - Column and row with correct title and units ( manipulated and responding variable) - Sufficient and systematic data (observational data ) Graph Title of the graph Both axes labeled with correct units Uniform scale All points plotted correctly Smooth curve and correct shape

Chart - Title of the chart - Both axes labeled with correct units - Uniform scale - Bars plotted correctly - Correct shape Diagram - No artistic - Big (suitable size), clear, - Label the diagram correctly and line for label cant be cross together - Neat and without broken lines Calculation - Work out accurate calculation - Wright formula - Replacement with correct data - Answer with correct unit

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vii) Interpreting Data - Based on the communicating data, able to state correctly the relationship between the variables viii) Relationship between space and time - Quantity and time (concentration, volume) - Relationship between manipulated / responding variable with time Example : The lower the concentration of enzyme so longer time used to hydrolysis starch ix) Predicting Give once value that may be true base on the trend / data before. x) Defining by operation - Base on experiment, refer observation - Including data, color, or time - Cant base on theory xi) Classifying Can group the answer base on the certain character 1.4.3.2 Question 2 i) Problem statement - In question form. - Relationship between manipulated and responding variable - End of sentence has question mark (?) ii) Aim of investigation - State the objective the experiment. iii) Hypothesis Make a statement of hypothesis by relating the manipulated variable with the responding variable and showing the specific relationship. iv) Variables - Manipulated variable - Responding variable - Controlling variable v) List of apparatus and materials Dont separate between apparatus and materials

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vi) Technique used State method and apparatus used to get responding variable Example : Measure and record final length of potato strips using ruler Measure and record the mass of food using beam balance Record final temperature of water using thermometer vii) Experimental Procedure or method List down the complete and correct technique used based on the following criteria: K1 : Technique of assembling the apparatus and materials to carry out the experiment K2 : Technique of fixing the constant variable K3 : Technique of changing the manipulated variable K4 : Technique of measuring the responding variables K5 : Technique of taking precautions to increase accuracy State precautionary in the experiment viii) Presentation of data - Record data in suitable table (blank table) - Title of column and row with correct unit (manipulated and responding variable) ix ) Conclusion - Write hypothesis again. Example : The higher the temperature the higher rate of transpiration ## Cant just write : hypothesis accepted

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1.5 PAPER 2 SPM 2009 Section A [60 marks] Answer all questions in this section 1 Diagram 1 shows the structure of a plant cell.

Diagram 1 (a) (i) Name organelle T [ 1 mark ] (ii) State two functions of the Golgi apparatus. 1. 2. [ 2 marks]

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(b) (i) Name the structure in the chloroplast which is involve in the light reaction of photosynthesis. . [ 1 mark ] (ii) Describe briefly the function of chloroplast in photosynthesis. [ 2 marks ] ( c) A housewife decided to make salted cabbage. She immersed the cabbage in concentrated salt solution for a certain period of time. (i) Draw a labeled diagram of the condition of one of the cabbage cells after being immersed in the concentrated salt solution.

[ 2 marks ]

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(iii) What has happened to the cell in the concentrated salt solution? Explain how this happened. .. .. .. .. .. [ 4 marks ]

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2. Diagram 2.1 shows processes in the formation of cell R.

Diagram 2.1

(a) (i) State the number of chromosomes in cell P and cell Q. P : .. Q : ... [ 2 marks ]

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(ii) Explain what has happened to cause the difference in chromosome number between cell P and cell Q. ...... .. ... [ 2 mark ] (b) (i) In which organ does cell R is formed? ... [ 1 mark ]

(ii) Name the process of the formation of cell R. ... [ 1 mark ]

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(c ) Diagram 2.2 shows the formation of a type of genetic disorder.

Diagram 2.2 ( i) State three characteristic of this genetic disorder. 1. ... 2. ... 3. ... [ 3 marks ]

(ii) Explain how a fault in the formation of cell R can cause this genetic disorder. ......... ..... ..... ..... [ 3 marks ]

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3. Diagram 3 shows the cross-sections of stem from plant P and plant Q which live in different habitats.

Plant P (a) (i) State the habitat of plant P and plant Q.

Plant Q

P : ... Q : [ 2 marks ]

(ii) Give one reason for the answers in 3(a)(i) . P : ... ... Q : ... ... [ 2 marks ]

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(b) (i) State the function of phloem. . [ 1 mark ]

(ii) State two differences in the structure of phloem and xylem.

Phloem

Xylem

(iii) Explain the importance of mitosis in the cambium to plant P. ..... ..... ..... [ 2 marks ]

(c) A type of plant lives in a swamp area with soft muddy soil and lack of oxygen. Explain the adaptations of the plant to survive in the habitat. ...... ...... ...... ...... [ 3 marks ]

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4. Diagram 4.1 shows a part of the human respiratory system.

P :

Q: ...

Diagram 4.1

(a) On Diagram 4.1, name P and Q. (b) Diagram 4.2 shows one stage in human breathing.

[ 2 marks]

Diagram 4.2 (i) State the stage of breathing shown in Diagram 4.2. . [ 1 mark ] 27

(ii) Explain the function of P in the stage of breathing in 4 (b)(i). ...... . [ 2 mark ] ( c) The diaphragm is unable to function. Explain how this affects the breathing mechanism. ...... ...... ...... ...... [ 3 marks ] (d) Gas exchange takes place across the surface of the alveoli. (i) Explain the importance of gas exchange in a human ...... ...... ...... [ 2 marks ] (ii) State two ways in which alveoli are adapted for efficient gas exchange. 1. 2. [ 2 marks ]

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5. Diagram 5 shows a human foetus in his mothers uterus

Diagram 5 (a) Name the structures labeled R and S. R : . S : [ 2 mark ]

(b) Explain the composition of the blood that flows through the blood vessels in S. ....... ....... ....... ....... [ 2 marks ]

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(c ) The structure R is needed to maintain the thickness of the uterine wall. Explain this statement. ....... ....... ........ [ 2 marks ] (d) (i) State the type of immunity the foetus gets from his mother. . [ 1 mark ] (ii) Explain the importance of structure R to body defence of the foetus. ...... ...... ...... ...... [ 3 marks ] (e) Explain why the foetus has s separate blood circulatory system from his mother. ........ ........ ........ [ 2 marks ]

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Section B Answer any two questions from this section 6. (a) Diagram 6 shows the characteristics and traits of five monohybrid crosses that Mendel made between garden pea plants (Pisum sativum).

Diagram 6

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Based on Diagram 6, (i) identify and name the characteristics and traits studied, [ 4 marks ]

(ii) calculate the ratio phenotype dominant to phenotype recessive for any three of the characteristics shown. [ 6 marks ] (b) A normal man ( XHY ) has a wife (XHXh) who a carried of haemophilia. Using a schematic diagram, explain the possible genetic combinations in their children. [ 10 marks ]

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7. (a) Diagram 7.1 shows a type of interaction between organisms.

Diagram 7.1

Name and describe the interaction shown in Diagram 7.1.

[ 4 marks ]

(b) A farmer found that when the number of mice increases, the yield of palm fruits decreases. Suggest how the farmer can control the population of mice without use of pesticides. Explain how the method works. [ 5 marks ]

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(c) Diagram 7.2 shows the roles of useful microorganisms in an ecosystem.

Diagram 7.2

(i) Based on Diagram 7.2, explain how the organisms maintain the content of compound X in the soil. [ 8 marks ]

(ii) Explain what will happen to the ecosystem if all decaying bacteria and fungi die. [ 3 marks ]

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8. Diagram 8.1 shows the same part of plant during a cool day and a hot day.

Cool day

Hot day

(a) (i) Explain the condition of the plant during a cool day and hot day.

[ 5 marks ]

(ii) Explain why it is necessary for the plant to be in the different conditions as in 8(a)(i) on a cool day and a hot day [ 5 marks ]

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(b) Diagram 8.2 shows the movement of water molecules in a plant.

Diagram 8.2

Explain how water molecules move from the root to the leaf in the plant and then moves out into the atmosphere. [10 marks ]

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9. (a) Diagram 9.1 shows the pathway of a far molecule which passes through a system.

Diagram 9.1

Gall bladder, pancreas and small intestine are structures involved in processing of the fat molecule. Explain the processes which occur to the fat molecule until it can used by body cells. [ 10 marks ]

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(b) Diagram 9.2, 9.3 and 9.4 shows the physical condition of three people, which is related to their eating habits.

Diagram 9.2

Diagram 9.3

Diagram 9.4

Explain the relationship between eating habits and physical condition of each person. [ 10 marks]

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1.5.2 PAPER 3 1. A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis. Diagram 1 shows the apparatus set-up to collect the gas produced when the aquatic plant received different intensities of light. The aquatic plant will illuminated from a lighted bulb of 40 watts for periods of 5 minutes at five different distance, d, from the bulb. At the end of each 5 minutes periods, a gas bubble was drawn into the capillary tube using syringe. The length of the gas bubble was measured using a ruler and recorded. The temperature in the water bath was maintained at 25OC throughout this experiment.

Diagram 1

39

Table 1shows part of capillary tube which contain the gas bubble collected at different light intensities.
Distance from the bulb (cm) Length of gas bubble (cm)

d = 50 cm cm

d = 40 cm cm

d = 30 cm

cm

d = 20 cm cm

d = 10 cm cm

Table 1

40

(a) Using the ruler provided on page 3, measure accurately the lengths of the gas bubbles for the different light intensities. Record the readings in the spaces provided in Table 1.

For examiners use

[3 marks] 1(a)

(b) (i) Based on Table 1, state two different observations. Observation 1: .. .. Observation 2: ... .... 1(b)(i) [3 marks]

(ii) State the inferences which corresponds to the observations in 1( b) (i). Inference from observation 1: . . Inference from observation 2 : . . 1(b )(ii) [3 marks]

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(c) Complete Table 2 based on this experiment. Method to handle the variable

For Examiners Use

Variable Manipulated variable Responding variable . Controlled variable .

. . .....

. . .. .. .. . Table 2
1(c)

[3 marks]

(d) State the hypothesis is for this experiment. . . . . [3 marks] 1(d)

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For Examiners Use

(e) (i)

Construct a table and record all the data collected in this experiment. Your table should have the following titles: Distance from the bulb Length of gas bubble Rate of photosynthesis Rate of photosynthesis = Length of gas bubble Time

1(e)(i) [3marks]

(e) (ii) Use the graph paper provided on the page 8 to answer this part of the question. Using the data in 1(e)(i) , draw the graph of the rate of photosynthesis against the distance from the bulb. [3 marks]

1(e)(ii)

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Graph of the rate of photosynthesis against the distance from the bulb

Rate of photosynthesis (cm / min)

Distance (cm)

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(f)

Based on the graph in 1(e)(ii), explain the relationship between the rate of photosynthesis and the distance from the bulb. .. ...... .. [3 marks]

For Examiners Use

1(g)

1(f)

(g) Base on the result of this experiment, state the operational definition for the process of photosynthesis. .... .... .... [3 marks] (h) Another group of students carried out the same experiment. The aquatic plant was exposed to a similar lighted bulb at a distance of 30 cm using 0.05% sodium hydrogen carbonate solution. Predict the outcome of this experiment. Explain your prediction. ... ... ... [3 marks] 1(h) 1(i) 1(g)

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(i)

Using the list provided below, in Table 3, classify the requirements to carry out the process of photosynthesis and the products obtained.

For Examiners Use

Water, Light, Oxygen, Glucose , Carbon dioxide, Chlorophyll

Requirements

Products

Table 3 [3 marks]

1(i)

Total

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1(g)

2. Situation 1A Housewife A uses warm water to wash her clothes using washing liquid which contains added enzyme. The cleaning is more effective. Situation 2 Using the same washing liquid as in situation 1, housewife B uses cold water to wash her clothes. The cleaning is less effective. Base on both situation, design a laboratory experiment to study the effect of temperature on the rate of enzyme reaction. The planning of your experiment must include the following aspects: Problem statement Aim of investigation Hypothesis Variables List of apparatus and materials Technique used Experimental procedure or method Presentation of data Conclusion

[17 marks]

END OF QUESTION PAPER

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MARK SCHEAM PAPER 2 : SPM 2009 MARKING SCHEME FOR BIOLOGY PAPER 2 SPM 2009 Question Mark Scheme 1(a)(i) Able to name T Sample answers Smooth endoplasmic reticulum/ endoplasmic reticulum (ii) Able to state the functions of Golgi apparatus Sample answer 1. modify protein/ carbohydrate/ glycoprotein/ phospholipid 2. packed them into secretory vesicles 3. transport protein/ carbohydrate/ glycoprotein/ phospholipid Any 2 (b)(i) Able to name the structure in the chloroplast which is involved in the light reaction of photosynthesis Sample answer Grana/ Granum (ii) Able to explain the function of chloroplast in photosynthesis Sample answer 1. absorb/ trap/ capture sunlight/ light energy 2. transformed/ converted/ changed it to chemical energy/ ATP (c)(i) Able to draw the labeled diagram 1. Correct diagram 2. Correct label Able to state the condition of the cell Sample answers F: Plasmolysed/ Plasmolysis/ cell becomes flaccid 1 1 1 2 1+1 2 1 1 1+1 2 Sub Total Mark Mark 1 1

(ii)

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Able to explain what has happen to the cell in the concentrated solution Sample answers 1. water diffuse out of the cell/ move out by the cell by osmosis 2. the cytoplasm and the vacuole will shrink/ becomes smaller 3. the cell membrane pull away from the cell wall 2(a)(i) Able to state the number of chromosomes in cell P and cell Q Answer P : 4/ 46 Q: 2/ 23 (ii) Able to explain on the number of the chromosomes in cell Sample answers 1. Spermatid/ Daughter cell/ Cell Q receives half the number of chromosome from primary spermatocyte/ parent cell/ Cell P 2. after undergoing meiotic division/ meiosis (b)(i) Able to name the organ in which cell R is formed Answer Testis (ii) Able to name the process of the formation of cell R Answer Spermatogenesis (c)(i) Able to state three characteristics in Down Syndrome Sample answer 1. 2. 3. 4. 5. 6. 7. Broad face Thick/ Protruding tongue Coarse/ straight hair Slanted eyes Broad forehead Flat nose Short neck

Max 3

Total 12 1+1 2

1+1

1x3

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8. Mentally retarded 9. Heart defect Any 3 (ii) Able to explain how a fault in the formation of cell R that can cause the genetic disorder Sample answer 1. Chromosome mutation 2. Homologous chromosome/ chromosome no. 21 fail to separate 3. During meiosis 4. Extra chromosome in the sperm/ Cell R 5. Fertilisation produced a zygote with 47 chromosomes 6. zygote contains 3 chromosomes no. 21/ trisomy at chromosome 21 7. the genetic disorder is down syndrome Any 3 3(a)(i) Able to state the habitats correctly Sample answers P : Terrestrial/ land area Q: Aquatic/ pond (ii) Able to give the reasons correctly Sample answer P : present of vascular bundle/ xylem and phloem Q: has many aerenchyma tissues/ air sacs (b)(i) Able to state the function of phloem Sample answer Transport organic materials/ product of photosynthesis (ii) Able to state two differences in the structures of phloem and xylem Sample answers Phloem Xylem Consists of sieve tubes/ Consists of xylem vessel/ companion cells tracheids Has sieve plate on the sieve Hollow tube 50 1+1 2 1 1 1+1 2 1+1 Max 3 3

Total 12 2

tube The cell wall is not thickened with lignin/ cell wall does not lignified Has protoplasm Any 2 (iii)

The cell wall is thickened with lignin/ cell wall is lignified No protoplasm

Able to explain the importance of mitosis in the cambium to plant P Sample answers 1. Produce new cell/ secondary xylem/ secondary phloem 2. Diameter/ size/ circumference/ girth of the stem increases 3. Stability increases/ for mechanical support/ longer lifespan/ to fulfill plant needs for water/ nutrients Any 2

1+1

(c)

Able to explain the adaptation of the plant to survive in the habitat Sample answers 1. F1 A root system that spread out widely/ Buttress root/ Proop root/ stilt/ cable root E1 Provides support 2. F2 Breathing root/ pneumatophores E2 Enable gaseous exchange/ respiration to occur 3. F3 Viviparity/ viviparous seedling E3 The seed stick vertically in the mud/ cannot wash away by wave Any 3 F or complementary E

Max 3

Total 12 1+1 2

4(a)

Able to name P and Q correctly Sample answer P : External intercostal muscle Q: Rib/ Ribcage

(b)(i)

Able to state the stage of breathing Sample answer Inhalation/ Breathing in

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(ii)

Able to explain the function of P in the stage of breathing Sample answer 1. External intercostal muscle / P contract 2. the ribcage move upwards/ outwards

1+1

(c)

Able to explain the effect on the breathing mechanism if the diaphragm is unable to function Sample answer 1. Less/ no change in volumn in the thoracic cavity/ lung 2. Less/ no change in air pressure in the thoracic cavity/ lung 3. Less/ no air exchange/ inhale/ expel/ exhale 4. (Resulting) difficulty in breathing (in/out) / shortness of breath

Max 3

(d)(i)

Able to state two importance of gas exchange in human Sample answer 1. To supply oxygen for (cellular) respiration// oxidized glucose 2. To excrete carbon dioxide/ release waste product

1+1

(ii)

Able to state two ways in which alveoli are adapted for efficient gas exchange Sample answer 1. 2. 3. 4. 5. Any 2 Large surface area/ Large TSA/V Thin wall/ one cell thickness Close contact with blood capillaries Moist surface Dense/ rich/ network of blood capillary

1+1

Total 12 1+1 2

5(a)

Able to name the structures labeled R and S Answer R Placenta S Umbilical cord

52

(b)

Able to explain the composition of blood that flows through the blood vessel Sample answer 1. (Umbilical) vein contain blood rich/ high in nutrient/ oxygen/ antibodies/ hormones 2. (Umbilical) vein carries blood from mother/ R to foetus/ S 3. (Umbilical) artery contain blood rich/ high in CO2/ waste products/ urea 4. (Umbilical) artery carries blood from foetus / S to mother/ R Any 2

Max 2

(c)

Able to explain the function of structure R to maintain the thickness of the uterine wall Sample answer 1. R/ Placenta produces/ secretes progesterone 2. which stimulate/ enhance the increase in the number/ development of blood vessel// Uterine wall highly vascularised

1+1

(d)(i)

Able to state the type of immunity the foetus gets from his mother Sample answer Passive Natural Immunity/ Natural Acquired Passive Immunity

(ii)

Able to explain the importance of structure R to the body defence of the foetus Sample answer 1. R allows the diffusion of antibodies 2. antibodies act on the pathogen/ neutralized toxin 3. foetus immune to the disease/ Prevent infection/ Preventing the foetus from getting disease

Max 3

(e)

Able to explain why the foetus has a separate blood circulatory system from the mother Sample answer 1. Protect the foetus from the higher blood pressure of the mother 53

Max 2

2. Prevent toxic substances in the mothers blood from entering the foetal blood 3. Prevent pathogen from entering the foetus 4. Prevent agglutination/ Prevent the mixing of blood Any 2 6(a)(i) Able to identify and name the characteristics and traits Sample answer Characteristics Traits Seed shape Round, wrinkled Seed colour Yellow, green Pod shape Inflated, constricted Pod colour Green, yellow Flower colour Purple, white Able to calculate the ratio of phenotype dominant to phenotype recessive Sample answer Substitution : 1 Correct answer: 1 Seed shape 5474 1850 2.96 1 or 2.96:1 or 3:1 1x4

Total 12 4

(ii)

2x3

Seed colour 6022 2001 3.01 1 or 3.01:1 or 3:1

Pod shape 882 299 2.95 1 or 2.95:1 or 3:1

Pod colour 428 54

152 2.82 or 2.82:1 1 Flower colour 705 224 3.15 1 (b) or 3.15:1 or 3:1 Max 10 10 or 3:1

Able to explain the possible genetic combinations in the children Sample answer Parental phenotypes Parental genotypes(2n) Meiosis (P1) Gametes (n) (P2) Fertilization (P3) Genotype F1 (P4) XHXH XHY Normal Man : 1 : XHXh XHY XH Y XH Xh Normal Man XHY x Carrier Woman XH Xh

Phenotype F1(P5) Normal Woman Phenotype ratio (P6) 1

Carrier Haemophiliac Woman Man 1 : 1

P7 Haemophilia gene is a recessive gene P8 Gene is link to X- Chromosome// sex-link gene P9 Male has only one X chromosome P10 Male has higher chances to have haemophilia P11- Haemophilia woman carry recessive gene on both X chromosome/ XhXh Any 10 7(a) Able to name and describe the interaction represented by Diagram 7.1 Sample answer

Total 20

55

Name of interaction (F) : Parasitism Description 1. (Parasitism is a relationship between to organisms in which) the parasite/ Rafflesia gain and 2. the host harm 3. parasite/ Rafflesia will absorb food substances from the tree/ xylem/ phloem/ vascular bundle 4. using modified roots/ haustorium 5. host will have less nutrients Any 3 (b) Able to explain how the farmer controls the population of mice Sample answer 1. 2. 3. 4. 5. 6. Any 5 (c)(i) Able to explain how the microorganism maintained the content of compound X in the soil Sample answer 1. Nitrogen fixing bacteria / Rhizobium 2. use nitrogen in the air to make nitrate/ ammonium compound 3. nitrogen produced are by absorbed by plants to make protein 4. when animal eat plants, the protein is transferred to animals 5. excretory nitrogenous substances/ urea/ waste products/ faeces 6. when plants/ animals die 7. they are decomposed by decaying bacteria 8. Breaks them down to ammonia 9. nitrifying bacteria/ Nitrosomonas sp convert ammonium compounds/ ammonia to nitrites 10. Nitrifying bacteria/ Nitrobacter convert nitrites to nitrates 11. Denitrifying bacteria converts nitrates to nitrogen 56 use biological control method involved prey-predator relationship/ interaction predators must be specific/ natural predators do not harm the oil palm fruits Owl/ snake/ predators are used population of mice decreases// Palm yield increases

1 Max 3 4

Max 5

Max 8

Any 8 (ii) Able to explain what will happen to the ecosystem if all the decaying bacteria and fungi die Sample answer 1. No breakdown/ decomposition of dead organic matters 2. Mineral ions e.g. nitrates cannot be released/ returned to the soil// nutrients cycle is stopped 3. Soil becomes infertile// less nutrient in the soil 4. Photosynthesis cannot takes place Any 3 8(a)(i) Able to explain the condition of the plant on a cool day and on a hot day Sample answer Cool day 1. The plant is firm and upright/ all leaves open 2. The plant cells are turgid 3. Enough/ plenty of water in the plant Hot day 4. The plant wilts/ all leaves close 5. Evaporation/ transpiration increase 6. Rate of water lost is higher than rate of water gains 7. Cells in the plant becomes flaccid Any 5 (ii) Able to explain the necessity for the plant to be in the condition shown Sample answer Cool day 1. All leaves open to increase the surface area 2. Absorption of light/ photosynthesis is maximal Hot day 3. Leaves closed to reduce the surface area exposed to sunlight 4. so reduces the rate of transpiration 5. Stomata are also closed/ less exposed 6. prevents plant from dying up/ excess loss of water 57

Max 3

Total 20 Max 5 5

Max 5

Any 5 (b) Able to explain the movement of water molecules from the root to the leaf and then out into the atmosphere Sample answer 1. Concentration of water in the cell is lower than the concentration of water outside the cell 2. water diffuses into the cell by osmosis 3. cell (sap) becomes hypotonic to the adjacent cell 4. water diffuses into the adjacent cell by osmosis 5. osmosis goes on until the water molecules reaches the xylem vessel/ cortex 6. root pressure in the root pushes water up the xylem 7. Cohesive force between water molecules draw up water in the xylem vessels 8. Adhesive force between water molecule and wall of xylem vessel 9. generate by capillary action 10. by the aid of transpiration pull 11. water evaporates from the surface of the mesophyll cells into air spaces 12. the water evaporates into surrounding through stomata 13. the water is lost from mesophyll cell is replaced by water in thexylem Any 10 9(a) Able to explain the processes which occur to the fat molecule Sample answer Digestion D1. Digestion/ breakdown of fats/ lipids start/ occur in duodenum D2. Bile salt stored in gall bladder// Bile produce by the liver D3. Emulsifies fats/ lipids into droplets D4. Pancrease secretes lipase D5. lipase hydrolyse lipids droplets into fatty acids and Glycerol// Lipids Fatty acids + Glycerol Max 10 Max 10 10

Total 20 10

Absorption A1. Absorption take place in small intestine/ ileum A2. Fatty acid and glycerol are absorb into lacteals/ villus 58

A3. In lacteal condensation of fatty acid and glycerol form lipids A4. lipid is transported via lymphatic vessels/ system A5. into the blood stream/ system/ subclavian vein Assimilation S1. Assimilation takes place in the cells S2. Cells use lipids to form complex compounds/ to synthesis enzymes/ hormones Any 10 (b) Able to explain the relationship between the eating habits and the health Sample answer Diagram 9.2 H1. Healthy person H2. Maintain body mass/ take balance diet H3. Take correct/ sufficient amount of carbohydrate to provide energy to the body H4. Correct amount of fats to provide energy H5. Correct amount of protein to produce new cells/ repair cells H6. Take a lots of water to avoid constipation Diagram 9.3 G1. Obesity G2. Imbalanced between food intake and energy used G3. Excessively intake of food rich in fats/ carbohydrates Diagram 9.4 K1. Anorexia nervosa K2. Severe/ serious loss in body weight K3. Starve continuously/ skip meals/ Refuse eat because afraid of gaining weight K4. Less energy taken in than body requires Any 10 Total 20 10 10

END OF MARK SCHEME PAPER

59

1.6.2 MARKING SCHEME BIOLOGY PAPER 3 SPM 2009 Question1 1(a) [KB0602 Measuring Using Number] Marks Mark Scheme Able to record all five readings accurately. Sample answer: Distance from the bulb (cm) 50 40 30 20 10 Able to record at least three readings accurately 3 to 4 readings Able to record at least one readings accurately 1 1 to 2 readings No response or incorrect respone Length of gas bubble (cm) 1 + 0.1 2 + 0.1 3 + 0.1 4 + 0.1 5 + 0.1

1 (b) (i) [KB0601 - Observation] Score Criteria Able to state two different observations correctly. Sample answer: 1. At distance of 10 cm from the bulb, the length of gas bubble is 5 cm. 2. The length of the gas bubble is 1 cm when the distance from the bulb is 50 cm. 3. As the distance from the bulb increase, the length of gas bubble decrease. 4. At distance of 10 cm from the bulb, the length of gas bubble is longer than the length of gas bubble obtained at distances 50cm / 40cm / 30cm / 20 cm ( correct corresponding observation )

60

Able to state one correct observation and one less accurate observations or able to state two inaccurate observations Sample Answer: 2 1. At distance of 10 cm from the bulb, the length of gas bubble is long. 2. At distance of 50 cm from the bulb, the length of gas bubble is short. 3. The length of gas bubble obtained at a distance of 10 cm and 50 cm from the bulb are different. 4. The length of the gas bubble is influenced by the distance from the bulb. Able to state only one correct observation or two different observations at idea level (id). 1 Sample Answer (idea level):
1. 2. 3. 4. Gas bubble is produce Gas bubble has different length The length is increasing / decreasing The highest of gas bubble is 5 cm

No response or wrong response. Scoring Score 3 2 1 Correct 2 1 1 1 Inaccurate 1 2 1 1 Idea 1 2 1 1 Wrong 1 1 1

61

1 (b) (ii) [KB0604 Making inference] Score Able to state two inferences Note : Inference must match observation

Criteria

P1 : Light intensity P2 : Gas produce / rate of photosynthesis Sample Answers: 3 1. At (10 cm), high light intensity course more gases to be produce / high rate of photosynthesis. 2. At (50 cm) low light intensity course less gas to produced / released / low rate of photosynthesis 3. The lower the light intensity, less gas is produced / the lower the rate of photosynthesis // inversely 4. The aquatic plant at distance 10 cm has higher light intensity produces more gas than the aquatic plant at the distance 50 cm / 40 cm / 30 cm / 20 cm Able to state one correct inference and one inaccurate inference. Or able to state two inferences inaccurately Sample answers: 2
1. (At 10 cm) more gas produced, height rate of photosynthesis 2. (At 50 cm), less gas is produced / low rate of photosynthesis // low light intensity. 3. (As the distance from the bulb increases), the lower the light intensity // less gas is produced / lower the rate of photosynthesis // inversely 4. At 10 cm high light intensity course oxygen to be produced.

Able to state only one correct inference or able to state two inferences at idea level. Sample Answer: 1. The length of gas bubble is influenced by the light intensity / rate of photosynthesis. 2. Plant carry out photosynthesis. 3. No response OR wrong response. Scoring: Score 3 2 1 Correct 2 1 1 Inaccurate 1 2 62 Idea 1 2 Wrong -

1 -

1 1 -

1 1

1 1 1

1 (e) [KB0610 Controlling Variables] Score Criteria Able to state all 3 variables and the methods to handle the variable. Sample Answer : Method to handle the variable correctly Variables Manipulated variable: Distance between the bulb / Place the bulb / light source at different light source and plant / distance from the plant// distance from the bulb // light intensity Used different light intensity Responding variable : The length of gas bubble // the rate of photosynthesis Use a ruler to measure and record the length of gas bubble produced // Calculate the rate of photosynthesis using the formula : length of gas bubble Time / 5 minutes ** Accept :measured and recorded Constant variable: 1. Percentage of sodium hydrogen carbonate solution 2. Type of plant / aquatic plant 3. Voltage / power of the bulb 4. Temperature of the water bath ** Reject : time taken 2 1 Able to state 2 of any variables and method to handle the variables. 4 5 ticks Able to state 1 of any variables and method to handle the variable
1. Fix the concentration of sodium hydrogen carbonate solution 0.02% 2. Use the same plant / aquatic plant 3. Use bulb of the( same power ) is 40W 4. Fix the temperature of water bath 25oC

63

2-3 ticks No response or only one criteria correct. 0 1 tick

1 (d) [KB0611 Making Hypothesis] Score Criteria Able to make a hypothesis base on the following aspects: P1 : Stating manipulated variable.( light intensity / distance from the bulb) P2: Stating responding variable (Rate of photosynthesis / length of the bubble) H : Relationship 3 Sample Answer : 1. As light intensity increases, the rate of photosynthesis increases./ (viseversa) 2. As distance of the plant from the bulb increases, the length of air bubble increases./ (vise-versa) Able to state a hypothesis relating the manipulated variable and the responding variable less accurately. 2 Sample Answer: 1. As light intensity increases, photosynthesis increases 2. The length of gas bubble depends on the distance of the plant from the bulb Able to state a hypothesis relating the manipulated variable at idea level. Sample Answer : 1
1. Light intensity affects / influences photosynthesis 2. Light intensity affects / influences the gas produced / released 3. As the rate of photosynthesis increase, the light intensity increases

No response or wrong response

64

1 (e) (i) [KB0606 Communication] Score Criteria Able to construct a table based on the following all three aspects: 1 : The 3 titles with correct units - 1m 2 : The data for distance and length. - 1m 3 : The rate of photosynthesis - 1m Sample answer : Distance (cm) 3 10 20 30 40 50 Length of gas bubble (cm) 5 4 3 2 1 Rate of photosynthesis (cm/min-1) 1.0 0.8 0.6 0.4 0.2

2 1 0

Any two aspects correct Any one aspect correct No response or wrong response.

1 (e)(ii) [KB0612 Relationship between space and time] Score Criteria Able to draw the graph correctly with the following aspects: P(paksi) 3 : Axes : Uniform scales on both horizontal and vertical axis 1 mark - 1 mark

T (titik ) : Points : All points plotted correctly

B ( bentuk ) : Curve : Able to join all the points to form a smooth graph / passing 65

through all point -ve gradient 2 1 0 Any two correct. Any one correct No response or wrong response.

- 1 mark

1 (f) [KB0608 Interpreting Data] Score Criteria Able to explain the relationship between the rate of photosynthesis and the distance based on the graph and the following aspects: P1- State the relationship P2 Light intensity P3 - Gas / Oxygen / air bubble produce. 3 Sample answer : 1. As distance increases, the rate of photosynthesis decreases because the light intensity decreases and less gas bubble produce // inversely. 2. The rate of photosynthesis in inversely proportional to the distance of the bulb, because light intensity decreases and less gas bubble produces. ** If graph gradient +ve automatic P1 reject. Able to interpret the relationship less accurately Sample answer: 2
1. As the distance increases, the rate of photosynthesis decreases because less

gas bubble are produced / light intensity increases.

Able to interpret the relationship at idea level. 1 1. As the distance increases, the rate of photosynthesis decreases . 2. As the distance increases, less gas bubbles are produced. 3. As the distance increases, the lower the light intensity No response or wrong response.

66

1 (g) [KB0609 Defining by Operation ] Score Criteria Able to define operationally the process of photosynthesis based on the following aspects: P1 : Occur in aquatic plant / water plant P2 : Requires light from the bulb / sodium hydrogen carbonate solution and Produce gas bubbles. 3 P3 : The length of gas bubble / rate of photosynthesis is influenced by light intensity / distance from the bulb // hypothesis ststement. . Sample answer: Photosynthesis is a process that takes place in an aquatic plant. It requires light from the bulb and sodium hydrogen carbonate solution and

produces gas bubbles. The rate of photosynthesis is influenced by light intensity / distance of plant from the bulb.

Any two aspects correct 2 2P Any one aspect correct.


1P

No response or wrong response

If theory : -

Green plant / plant .. chloroplast / chlorophyll Light , water, carbon dioxide (raw materials) Glucose , oxygen (product)

67

1 (h) [KB0605 Predicting] Score

Criteria

Able to predict the outcome of the experiment base on the following aspects: P1 : Correct prediction - The length of gas bubbles increases / longer // more than 3 cm / any value bigger than 3 cm// the rate of photosynthesis increases P2 : Effects - Rate of photosynthesis increases // more / oxygen is produce P3 : Reason 3 ( Concentration ) of sodium hydrogen carbonate solution increases / more carbon dioxide.

Sample answer: 1. The ( length) of gas bubble increases// more than 3 cm. The rate of photosynthesis increases / more than 0.6cm min -1 because the concentration of sodium hydrogen carbonate increases / more oxygen produced 2. The length of bubbles gas is 4 cm / the rate of photosynthesis is 1 cm min -1 because more carbon dioxide is used and the rate of photosynthesis increases Able to predict and explain the outcome of the experiment correctly with any two aspects : P1 and P2 or P1 and P3 P1 must have

Able to predict and explain the outcome of the experiment correctly with one aspect correctly. P1

No response or wrong response.

68

1 (i) [KB0602 Classifying ] Score Criteria Able to classify the requirements and product correctly: Sample answers Requirements 3 Water Carbon dioxide Chlorophyll Light No wrong 2 1 0 1wrong 2 wrong No response or wrong response Product

Glucose Oxygen

SCORE TOTAL - 11 X 3 = 33 marks

69

Mark scheme Question 2 Paper 3 2009. Aim Of Investigation / Objective : To study the effects of temperature on the rate of enzyme reaction.

KB061201 ( KB061203 Statement of Identified Problem) Score Criteria Able to state a problem statement relating the manipulated variable with the responding variable correctly. P1 : MV / temperature P2 : RV / Rate of enzyme reaction P3 : Question form and have question mark (?) Sample Answer : 1. What are the effects of temperature on (salivary) amylase activity on starch? 2. What is the effects of temperature on the rate of enzyme reaction? 3. Cool water or warm water can help the cleaning more effective? Able to state problem statement inaccurately . 2 Sample Answer:
1. What are the effects of temperature on enzyme? 2. The rate of enzyme reaction is affected by the temperature.

Able to state a problem statement at idea level. 1 Sample Answer:


1. Enzyme is influence by temperature?

No response or wrong response

70

KB061202 ( KB061203 Making Hypothesis ) Score Criteria Able to state a hypothesis relating the MV to the RV correctly P1 : (MV) P2 : (RV) H : Relationship Sample Answer : 1. When the temperature increase the rate of amylase / enzyme reaction (on starch) increase 2. When the temperature increase the rate of enzyme reaction decrease Able to state a hypothesis inaccurately Sample Answer : 2 1. 2. When the temperature increase the rate of reaction increase The rate of reaction of enzyme reaction is optimum at 40oC / 37oC

Optimum + value of temperature Able to state a hypothesis at idea level. Sample Answer: 1. The temperature affects the enzyme reaction 2. The enzyme reaction is optimum at 40oC / 37oC 3. When the temperature optimum enzyme reaction is maximum Optimum + value of temperature No response or wrong response

KB061203 Planning Investigation ( KB061203-Controlling variable ) Score Criteria Able to state three variables correctly: Sample answer: Manipulated variable: Temperature ( of medium of reaction) Responding variable : 1. The rate of enzyme reaction (catalysed by salivary amylase ) 2. The time taken for the completion of the hydrolysis of starch // time 71

taken for reaction of enzyme. Controlled variable: 1. Volume of amylase / saliva 2. Concentration of amylase 3. Volume of starch suspension 4. Concentration of starch suspension 5. pH value 6. Type of enzyme KB061205 ( KB061203-Listing of Materials and Apparatus) Score Criteria Able to list all the important apparatus and material correctly Sample answer: Apparatus : Beaker, test tube, thermometer, syringe, stop watch, Bunsen burner and tripod stand, wire gauze. 3
Materials : 1% *starch suspension // albumen // dirty cloth, *amylase suspension pepsin// washing liquid, water, ice cube, iodine solution // any reagent for food test. 6A + 5M Must have substrate and enzyme

Able to list at least 4-5 apparatus and at least 3 materials correctly 2


4-5A + 3M (name enzyme, name substrate , any other M)

1 0

Able to list at least 3 apparatus and at least 2 materials correctly 3A + 2M (name enzyme, name substrate ) No response or incorrect response To get ( 05) must have 1A + 1M

KB061203 Planning Investigation ( Technique - B1 = 1m ) Score Criteria Able to state suitable technique used for the experiment Bonus 1: 1M Sample answer: Using stopwatch to record the time taken for the starch to disappear / complete the hydrolysis of starch / (any food test) // Calculate the rate of enzyme reaction using the formula 1 t ( minutes) 72

KB061204 ( KB061203-Method / procedure of investigation) Score Criteria Able to describe the steps of the experimental correctly: Sample Answer: 1. Use a syringe to put 5 ml of 1% starch (suspension) into each of test 3 tube, labeled A1, B1,C1, D1 and E1. 2. Use a second syringe to put 2 ml of saliva / amylase solution into each test tube, labeled A2, B2,C2, D2 and E2 3. Immerse test tube A1 and A2 into water bath 4. After 5 minutes of immersion, pour the starch suspension from tube A1 into test tube A2 / mix starch and enzyme / Stir the mixture using a glass rod 5. Start the stopwatch immediately 6. Use a dropper to put a drop of mixture from test tube A2 onto the iodine solution / iodine test in groove of the white tile. 7. Repeat the iodine test for every minutes for 10 minutes. 8. Rinse the dropper after each sampling 9. Record the time taken for the completion of the hydrolysis of starch, that is when the mixture gives a negative iodine test. 10. Keep the test tubes with the mixture in their respective water baths. Throughout the experiment/ maintain the water bath at the respective temperature. 11. Repeat the experiment with the temperature 200C, 400C. 12. Record the result in the table. ** Cold water : 1oC 25oC Warm water : 30oC 40oC ** Reject : 0oC , hot water K1 Step 1,2,3,4,5,6,7 (preparation of materials and apparatus) any 4K1 steps K2- Step 1, 2 ( operating fix variable) (any one) K3 Step 9,12 ( operating responding variable) (any one) K4 Step 11 (operating manipulated variable ) K5 Step 8, 10, rinse the mouth before collecting saliva, repeat the experiment to get average reading ( any one ) 2 1 0 Able to state five K Able to state 3-4 K Able to state 2 K No response or wrong response 1K - Procedure

73

KB061203 Planning Investigation ( KB061203-Data Presentation B2 =1m) Score Criteria Able to construct a table of result with units Bonus 2 : 1m Sample answer: Temperature / oC Time taken for the Rate of reaction starch to disappear / 1 minutes t ( minutes)

KB061203 Planning Investigation ( KB061203-Conclusion ) Score Criteria Able to make the right conclusion: Sample answer: 1. When the temperature increase, the rate of amylase reaction increases until 37oC. 2. The rate of amylase reaction is highest at 37oC KB061203 - Planning ( Planning for investigation) Score Criteria Able to state 7-9 planning investigation of experiment following: Scoring Criteria: Problem Statement Objective of investigation Hypothesis Variables 3 List of apparatus and materials Technique used Experimental procedure Presentation of data Conclusion Scoring Criteria : 2 State 4 - 6 items Scoring Criteria: 1 State 2-3 items No response or wrong response 0 Or 0-1 items Mark: 3 X 5 = 15 marks B1 = 1 mark( technique) B2 = 1 mark( Data presentation) TOTAL = 17 marks END OF MARKING SCHEME 74

PAPER 3 (EXERCELANCE STUDENTS ANSWER) 1. A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis. Diagram 1 shows the apparatus set-up to collect the gas produced when the aquatic plant received different intensities of light. The aquatic plant will illuminated from a lighted bulb of 40 watts for periods of 5 minutes at five different distance, d, from the bulb. At the end of each 5 minutes periods, a gas bubble was drawn into the capillary tube using syringe. The length of the gas bubble was measured using a ruler and recorded. The temperature in the water bath was maintained at 25OC throughout this experiment.

Diagram 1

75

Table 1shows part of capillary tube which contain the gas bubble collected at different light intensities.
Distance from the bulb (cm) Length of gas bubble (cm)

d = 50 cm

1.0 cm

d = 40 cm

2.0 cm

d = 30 cm

3.0 cm

d = 20 cm

4.0 cm

d = 10 cm 5.0 cm

Table 1

76

(a) Using the ruler provided on page 3, measure accurately the lengths of the gas bubbles for the different light intensities. Record the readings in the spaces provided in Table 1. [3 marks] 1(a) (b)(i) Based on Table 1, state two different observations. Observation 1: The length of gas bubble is 1.0cm when the distance of the light bulb is 50cm.

Observation 2: The length of gas bubble is 5.0 cm when the distance from the light bulb is 10 cm. [3 marks] (ii) State the inferences which corresponds to the observations in 1( b) (i). Inference from observation 1: The rate of photosynthesis is the lowest due to low light intensity provided to the aquatic plant. Inference from observation 2 : The rate of photosynthesis is the highest when distance from light bulb is 10cm due to high light intensity provided to the aquatic plant [3 marks]

1(b)(i)

1(b )(ii)

77

(c) Complete Table 2 based on this experiment. Method to handle the variable

For Examiners Use

Variable Manipulated variable The distance of aquatic plant from the light bulb (cm) Responding variable The length of gas bubble release (cm) Controlled variable Temperature of water bath (oC)

Use different distance of aquatic plant from light bulb is 10cm, 20cm, 30cm, 45cm and 50 cm for each experiment

By measure and record the length of gas bubble released by using a ruler, for each experiment

Fix the same temperature of water bath which is 25oC throughout the experiment Table 2
1(c)

[3 marks]

(d) State the hypothesis is for this experiment. The longer the distance of aquatic plant from the light bulb, the shorter the length of gas bubble released. [3 marks] 1(d)

78

(e) (i)

Construct a table and record all the data collected in this experiment. Your table should have the following titles: Distance from the bulb Length of gas bubble Rate of photosynthesis Rate of photosynthesis = Length of gas bubble Time

For Examiners Use

Distance from the bulb / ( cm) 10 20 30 40 50

Length of gas bubble / (cm) 5.0 4.0 3.0 2.0 1.0

Rate of photosynthesis / (cmmin-1) 1.0 0.8 0.6 0.4 0.2

1(e)(i)

[3marks]

(e) (ii) Use the graph paper provided on the page 8 to answer this part of the question. Using the data in 1(e)(i) , draw the graph of the rate of photosynthesis against the distance from the bulb. [3 marks]

1(e)(ii)

79

(f)

Graph of the rate of photosynthesis against the distance from the bulb 80 Based on the graph in 1(e)(ii), explain the relationship between the rate of photosynthesis and the distance from the bulb. The rate of photosynthesis increases as the distance from the bulb

(i)

Using the list provided below, in Table 3, classify the requirements to carry out the process of photosynthesis and the products obtained.

For Examiners Use

Water, Light, Oxygen, Glucose , Carbon dioxide, Chlorophyll

Requirements Chlorophyll Light Carbon dioxide Water

Products Glucose Oxygen

Table 3 [3 marks]

1(i)

Total

81 1(g)

PAPER 3 SAMPLE ANSWER (MODERATE STUDENTS) 1. A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis. Diagram 1 shows the apparatus set-up to collect the gas produced when the aquatic plant received different intensities of light. The aquatic plant will illuminated from a lighted bulb of 40 watts for periods of 5 minutes at five different distance, d, from the bulb. At the end of each 5 minutes periods, a gas bubble was drawn into the capillary tube using syringe. The length of the gas bubble was measured using a ruler and recorded. The temperature in the water bath was maintained at 25OC throughout this experiment.

Diagram 1

82

Table 1shows part of capillary tube which contain the gas bubble collected at different light intensities.
Distance from the bulb (cm) Length of gas bubble (cm)

d = 50 cm

1.0 cm

d = 40 cm

2.0 cm

d = 30 cm

3.0 cm

d = 20 cm

4.0 cm

d = 10 cm 5.0 cm

Table 1

83

(a) Using the ruler provided on page 3, measure accurately the lengths of the gas bubbles for the different light intensities. Record the readings in the spaces provided in Table 1.

For Examiners Use

[3 marks] 1(a) (b)(i) Based on Table 1, state two different observations. Observation 1: The length of gas bubble is 1.0cm when the distance of the light bulb is far.

1(g)

Observation 2: The length of gas bubble is shortest when the distance from the light bulb is 10 cm. [3 marks] (ii) State the inferences which corresponds to the observations in 1( b) (i). Inference from observation 1: The rate of photosynthesis is the lowest due to when the distance is longer from lighted bulb. Inference from observation 2 : The rate of photosynthesis is the highest when distance from light bulb is the nearest [3 marks] 1(b )(ii)

1(b)(i)

84

(c) Complete Table 2 based on this experiment. Method to handle the variable

For Examiners Use

Variable Manipulated variable The distance of aquatic plant from the light bulb (cm) Responding variable The length of gas bubble release (cm) Controlled variable Temperature of water bath (oC)

Use different distance of aquatic plant from light bulb is 10cm, 20cm, 30cm, 45cm and 50 cm for each experiment

By measure the length of gas bubble released by using a ruler, for each experiment

Fix the same temperature of water bath Table 2

[3 marks]

1(c)

(d) State the hypothesis is for this experiment. The increase the distance of aquatic plant from the light bulb, increase the rate of photosynthesis of aquatic plant. [3 marks] 1(d)

85

For Examiners Use

(e) (i)

Construct a table and record all the data collected in this experiment. Your table should have the following titles: Distance from the bulb Length of gas bubble Rate of photosynthesis Rate of photosynthesis = Length of gas bubble Time

Distance from the bulb / ( cm) 10 20 30 40 50

Length of gas bubble / (cm) 5.0 4.0 3.0 2.0 1.0

Rate of photosynthesis 1.0 0.8 0.6 0.4 0.2

1(e)(i)

[3marks]

(e) (ii) Use the graph paper provided on the page 8 to answer this part of the question. Using the data in 1(e)(i) , draw the graph of the rate of photosynthesis against the distance from the bulb. [3 marks]

1(e)(ii)

86

87 Graph of the rate of photosynthesis against the distance from the bulb

(ii)

Using the list provided below, in Table 3, classify the requirements to carry out the process of photosynthesis and the products obtained.

For Examiners Use

Water, Light, Oxygen, Glucose , Carbon dioxide, Chlorophyll

Requirements Chlorophyll Light Carbon dioxide

Products Glucose Oxygen Water

Table 3 [3 marks]

1(i)

Total

88 1(g)

EXAMPLE ANSWER (QUESTION 2 ) 2. Situation 1A Housewife A uses warm water to wash her clothes using washing liquid which contains added enzyme. The cleaning is more effective. Situation 2 Using the same washing liquid as in situation 1, housewife B uses cold water to wash her clothes. The cleaning is less effective. Base on both situation, design a laboratory experiment to study the effect of temperature on the rate of enzyme reaction. The planning of your experiment must include the following aspects: Problem statement Aim of investigation Hypothesis Variables List of apparatus and materials Technique used Experimental procedure or method Presentation of data Conclusion

[17 marks]

89

(EXERCELLENCE STUDENTS ANSWER) Problem statement : What is the effect of temperature on the rate of enzyme reaction? Objective of investigation: To investigate the effect of temperature on the enzyme reaction Hypothesis : The higher the temperature, the higher the rate of enzyme reaction Variables : Manipulated variable : Temperature Responding variable : Rate of enzyme reaction Fix variable : Volume of starch Materials and apparatus: Boiling tube, beaker, thermometer, Bunsen burner, measuring cylinders, stop watch, syringes, tripod stand, wire gauze, glass rods, 1% starch suspension, 0.1% amylase solution, Benedict solution, ice cubes, water. Technique : Observe and record the time taken for Benedict solution to turn brick-red precipitate by using stopwatch. Procedure : 1. Fill boiling tube with 5ml of 0.1% starch suspension using measuring cylinder. 2. Put the boiling tube in a beaker containing ice cubes. 3. Fix the temperature of the ice cubes at 10oC. 4. Add 2 ml of Benedict solution into the test tube by using syringe. 5. Add 5 ml of 0.1% amylase solution into the test tube by using syringe and immediately start the stop watch. 6. Stop the stop watch when Benedict solution changes its colour. 7. Repeat step 1 to 6 by using different temperature of 0.1% starch suspension which is 20oC, 30oC , 35oC and 40oC.

90

8. Ensure that the temperature of starch suspension is fixed at its

required temperature by adding hot water or cold water.

Presentation of data :
Time taken for Benedict solution Rate of enzyme Temperature of starch to change colour to brick red reaction / minutes -1 suspension (oC) precipitate ( seconds)

10 20 30 35 40

Conclusion: The higher the temperature, the higher the rate of enzyme reaction. Hypothesis is accepted.

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(MODERATE STUDENTS ANSWER) Problem statement : How does different temperature of water affect the rate of enzyme reaction ? Objective of investigation: To investigate the effect of temperature on the rate of enzyme reaction Hypothesis : The optimum temperature for enzyme functioning effectively is 37oC. Variables : Manipulated variable : The temperature of water bath Responding variable : Time taken to hydrolyse albumen suspension from cloudy to clear Fix variable : Enzyme concentration Materials and apparatus: Test tube, measuring cylinder, thermometer, beaker, stopwatch, dropper, 5% pepsin, 10cm3 hydrochloric acid, albumen suspension, Bunsen burner, tripod stand, wire gauze, retort stand, water bath, ice cubes. Technique : Record the time taken for albumen suspension to be hydrolysed from cloudy to clear by using a stopwatch. Procedure : 1. 4 clean beakers are filled in water. Beakers are labeled as A, B, C, D. 2. Test tube are filled in 5 ml albumen suspension, 1% pepsin enzyme, 10 cm3 of hydrochloric acid by using measuring cylinder. 3. Beaker A contain test tube filled with albumen suspension, pepsin enzyme, hydrochloric acid. Then beaker A is put on tripod stand and cooled at temperature 25o by using ice cube. The action must be quickly. The thermometer record the temperature.

92

4. When the temperature falls from 30oC, the stopwatch is started. The colour changes of albumen is observed and recorded the time taken also recorded. 5. Beaker B is repeated which replaced the temperature 10 oC to 25oC by heating using Bunsen burner. The test tube is contained albumen suspension pepsin enzyme, hydrochloric acid put into the beaker. 6. Test tube in beaker C, D is repeated step 1, then heating to temperature 37oC and 45oC respectively by using thermometer. 7. The experiment is repeated twice to obtain the accurate result. Presentation of data : Beaker Temperature (oC) Time taken for albumen to hydrolysed from cloudy to clear 50 30 10 20 Rate of reaction ( 1/t) 0.02 0.03 0.10 0.05

A C B D

10oC 25 oC 37 oC 45 oC

Conclusion: The optimum temperature for albumen to hydrolysed is at 37oC.

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(POTENSIAL STUDENTS ANSWER) Problem statement : Warm and cold water make the cleaning of clothes more effective? Objective of investigation: To study the effectiveness of cleaning clothes with same enzyme and various type of water. Hypothesis : Warm water makes the process of cleaning clothes more effective. Variables : Manipulated variable : Type of solution Responding variable : The color changes to the iodine solution Fix variable : Volume of starch suspension and glucose solution Materials & Apparatus: Type of water, enzyme, beaker, stirrer, piece of cloth, stopwatch. Technique : Put piece of cloth in each beaker and take it out after a few minutes. Procedure : 1. Mix a warm water with enzyme put a place of cloth in the water 50 ml of starch suspension is measured using a measuring cylinder 2. Stir the mixture 3. Mix cold water with enzyme. 4. The cloth is put in the water.

94

Presentation of data : Effectiveness of enzyme Enzyme + warm water Enzyme + cold water Conclusion: Hypothesis is accepted.

95

EXAMPLE STUDENTS ANSWER (PAPER 2 SPM 2009) Section A [60 marks] Answer all questions in this section 1 Diagram 1 shows the structure of a plant cell.

Diagram 1 (a) (i) Name organelle T Smooth endoplasmic reticulum [ 1 mark ] (ii) State two functions of the Golgi apparatus. 1. Modify the protein produced by ribosomes into specific molecule 2. Pack the enzyme or protein molecule produced in a vesicle to be transported out of the cell. [ 2 marks]

96

(b) (i) Name the structure in the chloroplast which is involve in the light reaction of photosynthesis. Granum [ 1 mark ] (ii) Describe briefly the function of chloroplast in photosynthesis.
The grana in the chloroplast which contain chlorophyll absorb light energy from the sun to break up the water molecule into hydroxyl ion is then neutralizes and combine with carbon dioxide to produce or synthesis organic food molecules such as glucose while the hydrogen ion is used in dark reaction

[ 2 marks ]

( c) A housewife decided to make salted cabbage. She immersed the cabbage in concentrated salt solution for a certain period of time. (i) Draw a labeled diagram of the condition of one of the cabbage cells after being immersed in the concentrated salt solution.

[ 2 marks ] 97

(iii) What has happened to the cell in the concentrated salt solution? Explain how this happened.
The cell undergo plasmolysis. The concentrated salt solution is hypertonic to the cell sap in the cabbage cell. Therefore, water molecule from the cytoplasm and vacuole diffuse out of the cell to the salt solution by osmosis. As the result , the vacuole and cytoplasm lost water and shrink. The plasma membrane is pulled away from the cell wall. The cell wall does not change its shape. The salt solution diffuse into the cell of cabbage. The cell become flaccid and plasmolysed.

[ 4 marks ]

98

2. Diagram 2.1 shows processes in the formation of cell R.

Diagram 2.1

(a) (i) State the number of chromosomes in cell P and cell Q. P : 4 chromosomes Q : 2 chromosomes [ 2 marks ]

99

(ii) Explain what has happened to cause the difference in chromosome number between cell P and cell Q. Cell P undergoes a process of cell division called meiosis. The number of chromosomes of P is halved from gamete which is cell Q [ 2 mark ] (b) (i) In which organ does cell R is formed? Testes [ 1 mark ]

(ii) Name the process of the formation of cell R. Spermatogenesis [ 1 mark ]

100

(c ) Diagram 2.2 shows the formation of a type of genetic disorder.

Diagram 2.2 ( i) State three characteristic of this genetic disorder. 1. Slanted eyes, broad face. 2. Protruded tongue 3. Mental retardatation [ 3 marks ]

(ii) Explain how a fault in the formation of cell R can cause this genetic disorder.
There are 46 chromosomes in the parents cell. The parents cell undergoes meiosis to produce gamete which is cell R. During meiosis I, the number 31 chromosome fails to separate as a result produce two cells contain 22 chromosome and 24 chromosome respectively. During fertilization, the nucleus of the cell R that contains 24 chromosome fuse with the nucleus of normal ovum and result in a zygote that contains 47 chromosomes. This is called chromosomal mutation and person infected by the disease which is known as Downs syndrome.

[ 3 marks ]

101

3. Diagram 3 shows the cross-sections of stem from plant P and plant Q which live in different habitats.

Plant P (a) (i) State the habitat of plant P and plant Q. P : Land. Q : Water.

Plant Q

[ 2 marks ]

(ii) Give one reason for the answers in 3(a)(i) . P : P has vascular bundle when consists of phloem and xylem. Xylem provided support for the plant Q : Q has air sacs which provided buoyancy for the plant to float on water. [ 2 marks ]

(b) (i) State the function of phloem. 102

Transport food or organic compound from leaf to other part of plant. [ 1 mark ]

(ii) State two differences in the structure of phloem and xylem.

Phloem Has sieve tube

Xylem Has tracheid

Support by cellulose and Support by lignin pectin

(iii) Explain the importance of mitosis in the cambium to plant P. For secondary growth,. To provided mechanical support for the plant. The increase the girth of stem. To make the stem harder. [ 2 marks ]

(c) A type of plant lives in a swamp area with soft muddy soil and lack of oxygen. Explain the adaptations of the plant to survive in the habitat. The plant has pneumatophores . pneumatophores can absorb oxygen in air. The plant has cable roots or prop roots. To anchor the muddy soil. [ 3 marks ]

4. Diagram 4.1 shows a part of the human respiratory system.

103

P : Intercostal muscle

Q: Rib cage

Diagram 4.1

(a) On Diagram 4.1, name P and Q. (b) Diagram 4.2 shows one stage in human breathing.

[ 2 marks]

Diagram 4.2 (i) State the stage of breathing shown in Diagram 4.2. Exhalation [ 1 mark ]

104

(ii) Explain the function of P in the stage of breathing in 4 (b)(i). Internal intercostals muscle (P) will relax and work together with external intercostals muscle that contract to move the rib cage upward and outward during inhalation. [ 2 mark ] ( c) The diaphragm is unable to function. Explain how this affects the breathing mechanism. If the diaphragm is unable to function, breathing mechanisms may be difficult to function properly as the diaphragm is needed to contract and relax regularly for inhalation and exhalation. Contraction and relation of diaphragm will not occur. [ 3 marks ] (d) Gas exchange takes place across the surface of the alveoli. (i) Explain the importance of gas exchange in a human Gas exchange allow the respiratory gases like oxygen to be transported into the body and carbon dioxide to be eliminated from the respiratory system. This important to human for survive [ 2 marks ] (ii) State two ways in which alveoli are adapted for efficient gas exchange. 1. Moist in the surface 2. The alveoli is in numerous the increase the surface area. [ 2 marks ]

105

5. Diagram 5 shows a human foetus in his mothers uterus

Diagram 5 (a) Name the structures labeled R and S. R : Placenta S : Umbilical cord [ 2 mark ]

(b) Explain the composition of the blood that flows through the blood vessels in S. The umbilical vein carries oxygen, hormone, antibodies and nutrients from the mothers blood to the foetus to enable healthy growth of foetus. The umbilical artery carries carbon dioxide and waste materials from foetus to the mothers blood for to removal of unwanted substances from the foetus blood. [ 2 marks ]

106

(c ) The structure R is needed to maintain the thickness of the uterine wall. Explain this statement. R secrete hormone progesterone. Progesterone help in

maintaining the thickness of the endometrium wall so that the uterine lining will not break down [ 2 marks ] (d) (i) State the type of immunity the foetus gets from his mother. Nature passive immunity [ 1 mark ] (ii) Explain the importance of structure R to body defence of the foetus. The body of foetus still unable to produce its own antibodies to fight against pathogen. The antibodies from the mother will flow into the foetus blood through the blood vessels in umbilical cord. This help the foetus to acquire temporary immunity to fight against diseases or pathogens while it still in the mothers worm. The immunity can also protect the foetus for a few months after it is born. [ 3 marks ] (e) Explain why the foetus has s separate blood circulatory system from his mother. To form a selective barrier between mothers blood and foetus blood. Some toxic substances from the mothers blood cannot enter the foetus s blood to safeguard the foetuss life. It also gives protection to the foetus whereby the blood of the mother and foetus do not compatible with the foetus blood. It also prevent the high pressure of blood of the foetus. [ 2 marks ]

107

SECTION B ( SAMPLE STUDENTS ANSWER) QUESTION 6

Sample 1
6 (a) (i)

Traits of the seed shape are around and wrinkled. Traits of seed colour are yellow and green. Traits of the characteristic, pod shape are inflated shape and constricted shape. Traits for pod colour are green and yellow and for flower colour are purple and white.

Sample 2 6 (a) (i) The characteristic studied are seed shape, seed colour, pod shape , pod colour and flower of the garden pea plant, Pisum sativum among the F2 generation.
-

The traits for seed shape of the pea plants are round seed and wrinkled seed. The traits for seed colour are yellow seed and green seed The traits for pod shape are inflated pod and constricted pod The traits for pod colour are green pod and yellow pod The traits for flower colour are purple flower and white flower.

108

6 (a) (ii) Phenotype : Dominant allele for seed shape, R (round) Recessive allele for seed shape , r ( wrinkled)

Ratio of R and r

= R:r

= 5747 : 1850 = 3 : 1

Phenotype : Dominant allele for seed colour, Y (yellow) Recessive allele for seed colour, y (green)

Ratio Y and y

= Y : y = 6022 : 2001 = 3 : 1

Phenotype : Dominant allele for pod colour , G (green) Recessive allele for pod colour , g (yellow)

Ratio G and g

= G : y = 428 : 152 = 3 : 1

109

Sample 1 6 (b) Parents : Phenotype Genotypes Genes

: normal male : : XH Y XH Y x

a carrier of haemophilia wife XH Xh XH Xh

Children : Normal female Normal male

XHXH

XHY

XHXh

XhY
Haemophiliac male

Carrier Heamopholiac female

They may have a probability of getting female and a normal male, a female carrier of haemophiliac and a haemophiliac male. They have the probablility of getting 50% of normal children, 25% of getting a child with haemophiliac carrier and 25% of getting a male haemophiliac.

110

Sample 2
6 (b) - XH represents the allele for normal blood clotting (dominant)

- Xh represents the allele for haemophiliac (recessive) - Y represents the male sex allele
Parents : Male Normal x

Female Carrier

Phenotype : Genotype : Gamete :

XH Y XH Y

XH Xh XH Xh

Genotype P2 : XHXH Phenotype : Normal female

XHY

XHXh

XhY
Haemophiliac male

Normal male Carrier Heamopholiac female

Ratio of normal children to haemophilia children = 3 : 1 Since allele for heamophilia is recessive, therefore a female need to have homozygous recessive allele (XhXh) to become a haemophiliac. A female with heterozygous allele (XHXh) is a carrier but is normal.

111

QUESTION 7 7(a)

It is a symbiosis interaction It is a parasitism The flower acts as a parasite and tree act as a host. The flower obtain nutrients from the tree (host) and causing damage or harm to the tree. The flower cannot synthesis its own nutrient In this interaction, one the flower gets benefit and other the tree get harm

7 (b)

The farmer can use biological control It is using the prey-predator relationship to decrease the mice population. The farmer can release the owl or snake into his farm The owl or snake will act as a predator to catch and eat the prey (mice) Therefore , the population of mice is decreasing, eaten by predator Meanwhile, the increasing population of the predator will future the decrease in the mice population cause there is no mice population anymore. Since the mice is absent, the palm fruits will not be eaten by the mice anymore. Thus, the yield of the palm fruits increase. ( The predators do not eat the palm fruits )

7 (c ) (i)

The plant cannot absorb the nitrogen directly from the atmosphere The nitrogen in the air is assimilated into nitrogen compound (compound x) This nitrogen fixation process is done by the (nitrogen-fixing bacteria)

112

The example of nitrogen-fixing bacteria are the nostoc sp. ( living in the soil) and the rhizobhium ( living in the roots of leguminous plant) This compound x ( nitrogen compound) is then converted into nitrites by nitrosomonas through nitrification. Nitrates then, in turn, is converted into nitrates by nitrobacter through nitrification process. This nitrates is ready absorbed by plants and store as plant proteins in the plant. As animals eat the plant, the plant proteins is converted into animal protein. The faces the death body of plants and animals will be decayed by the decomposers into ammonia. The nitrates in the soil are then returned to the atmosphere to maintain the content of compound x in the soil. The nitrates is converted into nitrogen in the air by denitrifying bacteria through denitrification process. The oxygen is needed by the bacteria, nitrogen is returned to the air This is all known as nitrogen cycle.

7(c ) (ii)

The faces and the death body of the plants and animals will not be able to decomposed They are remained on the ground, polluting the area. The plants surrounding may not get the nutrient (ammonia) because the faeces and death body are not converted into nutrients. The soil in that area are not fertile.

113

QUESTION 8 (a) (i) During a cool day, the rate of transpiration and water loss are slow. The stomata of the leaves open to anable gaseous exchange for photosynthesis during a cool day , the rate of photosynthesis is high. The leaf also stand upright to receive maximum amount of sunlight. The leaf cell is turgid During a hot day, the rate of transpiration and water loss is very high. The stomata of the leaves is closed to prevent water loss. The leaf also lays down because the water loss has cause the leave cell undergo plasmolysis and not turgid, cannot support the weight of the leaves. The rate of photosynthesis is lower because gaseous exchange does not take place. 8(a)(ii) It is necessary for the plant to be in different condition in a cool day and a hot day because plant can prevent water loss in this way. During a hot day, the stomata is closed to prevent water loss. However, this also prevent gaseous exchange and rate of photosynthesis is low. During a cool day, the stomata is opened to enable gaseous exchange. Although the stomata is opened, the rate of water loss is not high because the temperature is low. As a result, the plant can photosynthesis at maximum rate during a cool day without the danger of water loss. The plant can also survive the hot day by close the stomata prevent excessive water loss. 8(b)
There is water molecule exist inside the soil and called soil water. The cell sap of root hair cell is hypertonic to the soil water as a result,

the water is moved into the root hair cell by osmosis. Now, the cell adjacent to the root hair is hypertonic to the root hair cell the water move from root hair cell to adjacent cell then neighbouring cell until the water reach the xylem by osmosis.

114

However the root pressure alone is not sufficient to push the water up until the leave. The transport of water inside xylem of stem is aided by capillary action due to the small diameter of xylem vessel. The water column is continuous and do not break due to the adhesive and cohesive property of water molecule. There is water vapour inside the air space of a leaf. The water vapour is diffuse to the atmosphere through stomata. This cause the sponge mesopyll cell to evaporate through more water into the air space become more hypertonic and draws water from adjacent cell The adjacent cell then draws water from xylem vessel, creating a pulling force, which is called transpiration pull Transpiration pull helps the water to be transported to all parts of plant and leaves and then move out into the atmosphere.

This had cause a root pressure to push the water up of xylem in stem.

115

CHAPTER 1: TRANSPORTATION
- initiated and coordinated by pacemaker
P1 P4 P5

Sinoatrial nodes (SA Nodes)

Atria Wall
P3

Cause

Atrial wall contract

Effect

blood pumped into ventricles

SA nodes

Spread
P7

Generate electrical impulse


P2

Spread impulse

P8

P9

P10

AV
Impulse reach
P6

Bundle branches Bundle of His fibres Purkinje fibres

Throughout

Cause

Effect

Ventricles Wall

Ventrical Contract

Blood pump out of ventricles to lungs and body

- controlled by: i. parasympathetic nerve slow down the pacemaker ii. sympathetic nerve speed up the pacemaker iii. hormone e.g: adrenaline

Diagram: The pumping of the Heart

D1

116

Change of BP

Baroreceptor } location: arch of aorta and carotid arteries

BP increases

Baroreceptor Cardiovascular centre

BP decreases

Weaker cardiac muscles contraction Relax and widening (vasodilation) To lower the resistance of blood flow

Heart Smooth muscles of the arteries

Stronger cardiac muscles contraction Contract and narrowing (vasoconstriction) To higher the resistance of blood flow

Normal BP Diagram: How blood pressure is regulated

D2 117

Clumped platelets Damage cells Clotting factors in the plasma

Form activator: Thromboplastin vit K & Ca+ Prothrombin Thrombin (active plasma proptein) Fibrinogen (soluble protein)

(inactive plasma protein)

Fibrin (insoluble protein)

Mesh (trapping RBC)

Scab Diagram: Mechanisme of Blood Clotting D3 118

1. blood that enter the arterial end of a capillary is under high hydrostatic pressure 2. cause some of plasma diffuse blood capillaries to space between cells. 3. form Interstitial fluid 4. Content: all blood components except RBC, plasma protein, albumin, globulin, fibrinogen, platelets

5. Function: exchange materials between blood capillaries and cells occurs 6. 85% of the interstitial fluid re-enter blood circulation at the end of the venule 7. 15% diffused into lymphatic vessels 8. to form lymph 9.
transported

ileum 11.

lacteal

diffuses

10. fatty acids glyserol

lymphatic system subclavian vein 12. blood circulatory system Diagram: Formation of Lymphatic system D4

119

1. Surface of mesophyll covered with a thin layer of water 2. heat from the sun cause water on the external surface evaporate 3. saturating the air spaces 4. concentration water vapour compare in surrounding

5. water vapour evaporate and diffuse out through stomata 6. movement of air carry away water vapour Diagram: Transpiration in Plant D5

120

Root pressure Root hair 2. water osmosis 1. surrounding soil is hypotonic 3. hypotonic Adjacent cell 4. diffuse Cortex 5. moves inward through: i. cytoplasm ii. vacuole iii. cell walls Endoderm is 6. has Cosparian strip block the water through cell wall Xylem vessel

7. moves inward through: i. cytoplasm ii. vacuole

8. Create a pushing force that result the inflow of water into the xylem root pressure Capillary action 9. Cohesive force between water molecules draw up water in the xylem vessels 10. Adhesive force between water molecule and wall of xylem vessel

Transpiration pull

11. water evaporates from the surface of the mesophyll cells into air spaces 12. the water evaporates into surrounding through stomata 13. the water is lost from mesophyll cell is replaced by water in the xylem Diagram: Transportation of water from soil to leaves D6

121

CHAPTER 3: COORDINATION AND RESPONSE


Voluntary action Stimuli Receptors Afferent neurone Involuntary action (Reflexes) a) Reflex arc (needle) Stimuli Receptors Afferent neurone b) Knee-jerk reflex (patellar) Stimulus Receptors Afferent neurone Spinal cord Efferent neurone D7 Effectors Response Spinal cord (interneurone) Efferent neurone Effectors Response Brain (Interneurone) Efferent neurone Effectors Response

Diagram: Transmission pathway of Information

122

1. Electrical impulses reaches presynaptic membrane 2. Triggers synaptic vesicles 3. release neurotransmitter 4. into synaptic cleft 5. neurotransmitter diffuses 6. bind to receptors 7. leads to generation of a new electrical signals

Diagram: Transmission of information across synapses D8

123

A. ULTRAFILTRATION 1. renal artery receive blood from aorta 2. high pressure arteriole larger than efferent arteriole 4. form high hydrostatic pressure 5. blood enter glomerulus 6. ultrafiltration takes place 7. fluids is called glomerular filtrate 8. have same composition with blood except no erythrocyte and plasma protein
1 2

C. SECRETION 1. Function eliminate waste products 2. Location: Distal convulated

tubule 3. Substances: H+, K+, NH3, urea, creatinine, toxins, drugs

3. maintained & enhanced because diameter of afferent

B. REABSORPTION Location Proximal tubule Loop of Henle Substances 100% of glucose & amino acid water 65% sodium ions Large quality of ions water Process involve Active transport Osmosis Passive transport Osmosis D9

Diagram: Formation of Urine

124

Negative feedback mechanisms Stimulate pituitary glands Secrete more ADH into blood Distal CT and collecting duct more permeable More water reabsorbed Less urine and concentrated

Osmoreceptor cell detect pressure increase Normal blood osmotic pressure decrease

decrease Normal blood osmotic pressure increase

Osmoreceptor cell detect pressure

Stimulate pituitary glands Secrete less ADH into blood Distal CT and collecting duct less permeable Less water reabsorbed More urine and dilute

Diagram: Osmoregulation by kidneys

D10

125

Chemoreceptor detect increases Normal blood glucose level decreases Chemoreceptor detect

Pancreas stimulated Secrete insulin i. glucose is used for cell respiration ii. excess glucose glycogen iii. excess glucose fat

decreases Normal blood glucose level increases

Pancreas stimulated Secrete glucagons i. rate of respiration decrease ii. glycogen glucose

Diagram: Homeostatic control of blood glucose level

D11

126

Thermoreceptor detect

increase Normal temperature (37C) decrease

Sweating heat is absorbed from skin to enable evaporation Vasodilation of smooth muscles in arterioles increase the amount of heat radiated and lost Hair shaft flatten warm air not trap against skin

decrease Normal temperature (37C) increase

Thermoreceptor detect

No sweating Vasoconstriction of smooth muscles in arterioles reduce the amount of heat radiated and lost Hair shaft raised trapping insulating layer of warm air Shivering of skeletal muscles heat is generated

Diagram: Homeostatic control of body temperature

D12

127

2. Auxin accumulate at the region with lower light intensity 3. Auxin diffuse to elongation region 4. stimulates the cells elongation 5. more auxin, the rate of cell elongation higher D13

1. Auxin is produced at coleoptile

Shoot

6. shoot bending toward the light Diagram: The role of auxins in phototropism

Shoot

1. light and gravity cause auxin transported to the lower side

2. accumulation of auxin cause cell elongate faster, shoot bending upward

3. accumulation of auxin inhibits cell elongation, root bending downward

Root

D14

Diagram: The role of auxins in geotropism

128

CHAPTER 4: REPRODUCTION AND GROWTH


Hypothalamus

Positive feedback

Pituitary gland

Negative feedback

Ovary High Oestrogen Day 12 14


and

Low Day 1 11 High Day 14 - 28 Uterus

Progesterone

Diagram: Regulating of hormonal in menstrual cycle D15

129

Day 1 11

Release Hypothalamus GnRH Pituitary gland

Release
&

LH

FSH

Follicle Inhibit Oestrogen & Progesteron Negatitive Feedback Oestrogen Relatively low

D16

130

Day 12 14

Positive feedback Hypothalamus

Secrete GnRH increases

Secrete

FSH increases & LH increases Cause Ovulation D17

Oestrogen increases

Day 14 28 FSH & LH increases

Stimulate Ovulation

Promote development Corpus luteum

Secrete Progesterone & Oestrogen

Negatitive Feedback

Inhibit FSH & LH

Hypothalamus & Pituitary gland

D18

131

PP

Projek Jawab Untuk Jaya


3

1. FSH is secreted stimulate the development of follicles (at the same time menstruation occurs) 2. follicles produce oestrogen but in a small amount, as the follicle larger, oestrogen increases 3. cause increase sectretion of GnRH
7

Hormone Hormone FSH FSH

Hormone Hormone LH LH

FSH increases
5

4. LH inreases

5. corpus luteum produce progesterone and oestrogen 6. implantation 7. if no implantation, corpus luteum degenerating

6 6

8 8

8. progesterone & oestrogen decreases

9. endometrium wall break down

D19 132

1. Pollen grain germinates 3. form pollen tube 4. pollen tube grows into the style

2. sugar stimulate germination Stigma


Tube nucleus

5. generative nucleus divides by mitosis 2 male gametes

7. 1 male nuclei + 2 polar nuclei triploid zygote 8. 1 male nuclei + egg cell diploid zygote Ovary Tube nucleus Integument 6. Penetrate the ovule through micropyle

Diagram: Double Fertilisation in Plant

D20

133

CHAPTER 5: INHERITENCE
Parental Phenotype (P1) Parental Genotype (P2) Tall (Heterozygote) Tt Tall (Heterozygote) Tt
Meiosis (P3)

Gametes

(P4)

t
Fertilisation (P5)

F1 Genotype F1Phenotype Genotype ratio Phenotype ratio Key:

(P6) (P7) (P8) (P9)

TT Tall 1TT 3Tall : : 2 Tt :

Tt Tall 1 tt

Tt Tall

tt Dwarf

1 Dwarf

(P10) T allele for tall T allele for dwarf Diagram: Schematic Diagram for monohybrid D21

134

CHAPTER 6: VARIATION
Bacterial chromosome Bacterium Plasmid 1. Isolation of plasmid DNA 2. Gene of interest is cut using an enzyme Cell containing gene of interest

Plasmid recombinant

3. Gene inserted into plasmid

4. Plasmid put into bacterial cell Bactrium recombinant Culturel medium 5. Recombinant bacterium is cultured

6. Identify bacteria carrying gene of interest

Diagram: Genetic Engineering 135

D22

PAST YEAR QUESTIONS 1. The following statements are on lymphatic system and blood circulatory system. The substances that come out from the blood capillaries into the body tissues will be reentered into the blood circulatory system Lymphatic system also function in transport products of digestion Explain the above statements to justify that lymphatic system is a complementary to the blood circulatory system. [10 Marks] [SPM 2006] Refer D4 2. Auxin is a plant hormone which helps in plant growth. Diagram 7.2 shows the growth of a shoot towards light.

Explain the role of auxin in the growth of the plant shoot as in Diagram 7.2. [4 marks] [SPM 2007] Refer D13

136

3. Diagram 8.2 shows the movement of water molecules of water molecules in a plant.

Explain how water molecules move from root to the leaf in the plant and then moves out into the atmosphere. [10 marks] [SPM 2009] Refer D6

137

4. Human Chorionic Gonadotrophin (HCG) has a similar role to luteinizing hormone (LH). The woman has a problem conceiving due to failure in ovulation. The woman becomes pregnant after a doctor has given her injections of HCG

Based on the above statement, explain how the HCG injections enable the process of pregnancy. [6 marks] [SPM 2005] Refer D17 5. Figure 7(c) shows the organs and glands involved in regulating the human body temperature.

A student skates on an ice skating rink. Based on figure 7(c), explain how regulation of the students body temperature occurs. [10 marks] [SPM 2004] Refer D12

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6. Starting from when the blood enters the kidney, explain how the waste products excreted from kidney as urine. [15 marks] [SPM 1988] Refer D9 7. A supervisor enters a frozen storage that use for keeping the food. Explain the physiology changes that occurs in the body to maintain his body temperature. [10 marks] [SPM 2001] Refer D12 8. Individual X had a medical check up. He told the doctor that his wound at his leg takes time for recovery. After the medical check up ,the doctor told him: i. His urine is positive for reducing sugar ii. His blood glucose level over the normal level iii. Are adviced to reduce the intake of rich carbohydrate food iv. To take insulin injection Based on your biological knowledge, write a report that explain the individual Xs condition. [8 marks] [SPM 2001] Refer D11 9. Menstrual cycle is controlled by hormones that released by pituitary gland and ovary. Married women who want to practice family planning may use the contraceptive pills. Describe how contraceptive pills act as a negative feedback to prevent pregnancy. [10 marks] [SPM 2002] Refer D16 & D18 10. In a family, the father has curly hair and the mother has straight hair. Based on your genetic knowledge, explain why all their children do not have straight hair. [10 marks] [SPM 1987] Refer D21

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11. Diagram 7.1 shows an electron micrograph of cellular components of human blood

Diagram 7.1 Based on Diagram 7.1, explain how platelets help to stop bleeding when a wound occurs. [ 4 marks ] [SPM 2008] Refer D3

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Answer all questions. Jawab semua soalan. The time suggested to complete each question is 45 minutes. Masa yang dicadangkan untuk menjawab semua soalan ialah 45 minit.

QUESTION 1 A group of biology students carried out an experiment to determine the content of carbon dioxide in exhaled air of a student after three different vigorous activities: 1: Climbing stairs to the first floor, 2: Climbing stairs to the second floor, 3: Climbing stairs to the third floor. A J-tube is used in this experiment. Sekumpulan pelajar biologi menjalankan satu eksperimen untuk menentukan kandungan karbon dioksida dalam udara hembusan seorang pelajar, selepas menjalankan tiga aktiviti cergas: 1: Mendaki tangga ke tingkat satu,2: mendaki tangga ke tingkat dua, 3: Mendaki tangga ke tingkat tiga. Tiub-J digunakan dalam eksperimen ini. Diagram 1 shows the initial length of air column in J-tube is 6.0 cm before potassium hydroxide (KOH) solution is used. The function KOH is to absorb carbon dioxide gas. Rajah 1 menunjukkan panjang awal turus udara dalam tiub-J iaitu 6.0 cm sebelum kalium hidroksida (KOH) digunakan. Fungsi KOH ialah untuk menyerap gas karbon dioksida.

6.0 cm

DIAGRAM 1 / RAJAH 1

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Table 1 shows the final length of air column in J-tube after treatment with potassium hydroxide (KOH) . Jadual 2 menunjukan panjang akhir turus udara dalam tiub J selepas dirawat dengan kalium hidroksida (KOH). O9Type of vigorous activities Jenis aktiviti cergas. Final length of air column after treatment with KOH (cm) Panjang akhir turus udara selepas dirawat dengan KOH (cm)

Climbing first floor/Mendaki tingkat satu

Air column /Turus udara

climbing second floor/ Mendaki tingkat dua Air column / Turus udara

Climbing tihird floor/ Mendaki tingkat 3 Air column / Turus udara

TABLE 1/ JADUAL 1

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(a) In table 1, record the final length of air column in J-tube after treatment with potassium hydroxide. Dalam jadual 1, catatkan panjang akhir turus udara selepas dirawat dengan kalium hidroksida. [3 marks] (b)(i) Based on table 1, state two observations from this experiment. Berdasarkan jadual 1, nyatakan dua pemerhatian daripada eksperimen ini. Observation1/ Pemerhatian 1 ... Observation 2/ Pemerhatian 2 [3 marks] (ii) State the inferences which corresponds to the observations in (b)(i) Nyatakan inference yang berkaitan dengan pemerhatian dalam (b)(i). Inference 1/ Inferens 1 ... Inference2/ Inferens 2

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(c) Complete Table 2 based on this experiment. Lengkapkan jadual 2 berdasarkan eksperimen. Variable Pembolehubah Manipulated variable Pembolehubah dimanipulasi .... .. Responding variable Pembolehubah bergerakbalas ........................................... Controlled variable Pembolehubah dimalarkan . . . Table 2/ Jadual 2 (d) State the hypothesis for this experiment. Nyatakan hipotesis eksperimen ini.

[3 marks]

Method to handle the variable Kaedah mengendalikan pembolehubah

..

..

[3 marks]

... [3 marks]

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(e)(i) Construct a table and record the result of the experiment which includes the following aspects: Bina satu jadual dan rekod keputusan eksperimen di mana mengandungi aspek berikut : Climbing to what floor activities Altiviti mendaki ke tingkat berapa Length of air column before and after treatment with KOH Panjang turus udara sebelum dan selepas rawatan dengan KOH Change in length of air column Perubahan panjang turus udara Percentage of carbon dioxide Peratus karbon dioksida Percentage of carbon dioxide = Change in length of air column Initial length of air column x 100% x 100%

Perubahan panjang turus udara Peratus karbon dioksida = Panjang awal turus udara

[3 marks]

145

ii)

Draw a bar chart to show percentage of carbon dioxide against activities on the graph paper provided. Lukis graf bar untuk menunjukkan peratus karbon dioksida melawan aktiviti cergas pada kertas graf yang disediakan. [3 marks]

f)

Based on a bar chart, explain the relationship between level of floor and the percentage of carbon dioxide released ?

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Berdasarkan graf bar , terangkan perhubungan antara tingkat yang didaki dan peratus pembebasan karbon dioksida? ... [3 marks] g) Climbing stair activity is repeated by increasing to fifth floors. Predict the percentage of carbon dioxide released. Aktiviti mendaki tangga diulang dengan meningkatkan tingkat, ke tingkat lima. Ramalkan peratus karbon dioksida yang dibebaskan. ... [3 marks] (h) Based on the result of this experiment, state the operational definition exhaled air? Berdasarkan keputusan eksperimen ini, nyatakan definasi secara operasi bagi udara hembusan? ... [3 marks]

(i) Using the list provided below, in the table 3, classify the requirements to carry out the experiment.

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Menggunakan senarai yang disediakan di bawah, dalam jadual 3, klasifikasikan keperluan untuk menjalankan proses eksperimen

J-tube, stair ,thermometer, potassium hydroxide solution, water, student. Tiub J, tangga,thermometer, larutan kalium hidroksida, air, pelajar.

Material
Bahan

Apparatus
Radas

Table 3 / Jadual 3

[3 marks]

148

Mark Scheme Question 1 No 1(a) Mark Scheme KB0603 Measuring Using Number Able to record all the three final length of air column after treatment with KOH correctly Sample answer
Type of vigorous activities Jenis aktiviti cergas. Final length of air column after treatment with KOH (cm) Panjang akhir turus udara selepas dirawat dengan KOH (cm)

Score

First floor Second floor Third floor Able to record any two heights correctly Able to record any one height correctly No response or incorrect response. (b) (i)

5.9 5.7 5.5

2 1 0

KB0601 - Observation Able to state any two correct observations based on the following criteria : K1 Level of the floor K2 - The final length of air column Sample Answer Horizontal observation: 1. The final length of air column after treatment with KOH is 5.9 cm when student climb to first floor 2. The final length of air column after treatment with KOH is 5.7 cm when student climb to second floor 3. The final length of air column after treatment with KOH is 5.5 cm when student climb to third floor Vertical observation : 4. When climbing to third floor, the final length of air column after treatment with KOH is the shorter/lower compared to first floor 3

149

Able to state one correct observation and any one inaccurate observation or able to state two inaccurate observations Sample answers of incomplete observation : (Has the level of floor, but no value of length but in qualitative) Horizontal 1. At first floor,the final length of air column after treatment with KOH is longer. 2. At third floor,the final length of air column after treatment with KOH is shorter. Vertical 3. Lower level of floor, has longer final length of air column after treatment with KOH (comparing) Able to state only one correct observation or Able to state two observations at idea level. Sample answer 1. In lower / higher of level floor, has longer/shorter final length of column after treatment with KOH. 2. Final length of air column is decrease. No response or incorrect response or one idea only Scoring Correct 2 1 1 1 Inaccurate 1 2 1 1 Idea 1 2 1 1 Wrong 1 1 1 Score 3 2 1 1

150

(b) (ii)

KB0604 - Making inference Able to make two correct inferences Sample answers Horizontal observation 1. When climbing to first floor, longer length of air column because exhale air contains small amount of carbon dioxide 2. When climbing to third floor, shorter length of air column because exhale air contains large amount of carbon dioxide 3 Vertical observation 3. When climbing lower level of floor, longer length of air column means small amount of carbon dioxide //vice-versa Notes : The inferences should be correspond to the observations. inference 1observation 1 - inference 2observation 2 0 mark if not correspond

Able to make one correct inference and one inaccurate inference or Able to state two inaccurate inferences Sample answers Inference (horizontal observation) 1. When climbing first floor, longer length of air column 2. When climbing third floor, shorter length of air column Inference ( vertical observation) 3. When climbing first floor level, longer length of air column compared to climbing third floor level//vice-versa Able to state only one correct inference or Able to state two inferences at idea level Sample answers 1. The air column contain carbon dioxide 2. The amount of carbon dioxide depends on climbing stair activities. 3. Climbing stair activities released carbon dioxide 1

151

No response or incorrect response Correct 2 1 1 1 (c) Inaccurate 1 2 1 1 Idea 1 2 1 1 Wrong 1 1 1 Score 3 2 1 0

KB0610-Controlling variables Able to state all 3 variables and 3 methods to handle each variable. Sample answer Variable Manipulated variable
Level of floor

Method to handle the variable Climbing at first, second and third floor Use different level of floor
Measure and record the length of air column after treatment with KOH using a ruler // Calculate the percentage of CO2 using formula = Change in length X 100 Initial length

Responding variable
Length of air column after treatment with KOH or // percentage of carbon dioxide

Controlled variable 1.Age of student 2. Location of the Stair

1. Use the same/ fix age of student 2. Use the same/ fix location of stair

All 6 ticks Able to state 4 to 5 ticks Able to state 2-3 ticks 152 2 1

No response or incorrect response or one tick only (d) KB0611-State hypothesis Able to state a hypothesis relating manipulated variable and responding variable correctly with the following aspect : P1 Manipulated variable level of floor/ climbing to what floor P2 Responding variable (Final) Length of air column after treatment with KOH/ percentage of carbon dioxide H - relationship higher // lower Sample answer 1. The higher/lower climbing the floor, the shorter/longer the air column after treatment with KOH 2. The content/ percentage of carbon dioxide in exhaled air of climbing stair to third floor is more than climbing stair to first floor Able to state a hypothesis relating the manipulated variable and the responding variable but less accurately. Sample answer 1. The floor of stair climbing affects the percentage of carbon dioxide/length of air column after treatment with KOH. Able to state one idea of a hypothesis Sample answer 1. Stair climbing affects the percentage of carbon dioxide (no P1 and relationship) No response or incorrect response If no P1 and P2, no mark for H KB0606 Communicating data (e) (i) Able to construct a table correctly with the following aspects : 1. Able to state the 3 titles with units correctly 2. Able to record all data for floor climbing and changes in length of air column correctly. 3. Able to calculate and record the percentage of carbon dioxide -T D -C 1- mark 1 mark 1 - mark 1 2 3

153

Sample answer The level of floor climbing Final Initial Length Length /cm /cm Changes in lenght of air column after treatment with KOH /cm 0.1 0.3 0.5 Percentage of the carbon dioxide / % 1.67 5.00 8.33

(First)1 (Second)2 (Third)3

6.0 6.0 6.0

5.9 5.7 5.5

Any two aspects correctly Any one aspect correctly No response or incorrect response (e) (ii) Able to draw a graph of the rate of yeast activity against the concentration of glucose which satisfies the following criteria: Axes (P) both axes are labelled and uniform scales, manipulated variable on horizontal axis, correct units. Points(T)- all points correctly plotted Shape(B)- all points are connected smoothly Sample Answer Refer graph 9
8 7 6 5 4 3 2 1 0

2 1 0

3
percentage of carbon dioxide

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Floor1

Floor2

Floor3

Any two aspects correctly Any one aspects correctly No response or incorrect response (f) KB 0608 Interpreting data Able to explain the relationship between the level of floor climbing activity and the percentage of carbon dioxide correctly based on the following criteria: R1- The level of floor climbing increased/decrease R2- rate of respiration// cellular respiration increase/decrease R3- More carbon dioxide released/ higher percentage of carbon dioxide Sample Answer 1. When the climbing stair increased/decreases the floor, 2. rate of respiration// cellular respiration increase/decrease 3. So the released of carbon dioxide/higher percentage of carbon dioxide is increased/ decreased Able to explain the relationship using any two criteria Able to explain the relationship using one criteria No response or incorrect response KB0605 - Predicting Able to predict correctly and explain the prediction based on the following criteria: K1 Percentage of carbon dioxide increases K2 more than 8.33 % K3- due to the rate of respiration is increase // more CO2 is released

2 1 0

2 1 0

155

Sample answer Contoh jawapan


Percentage of carbon dioxide increases more than 8.33 % due to the rate of respiration is increase// more Carbon dioxdixe is released

Any two criteria stated Any one criteria stated No response or incorrect response

2 1 0

(g)

KB0609 Defining by operation Able to state the definition of exhaled air operationally, complete and correct based on the following criteria: D1- Exhaled air contains carbon dioxide (Fact) D2- that cause the length of air column to be shorter or longer after treatment
with KOH (RV)

D3- affected by different level of floor stair climbing (MV ) Sample answer
Exhaled air contains carbon dioxide (D1) that cause the length of air column to be shorter or longer after treatment with KOH (D2) affected by different level of the floor stair climbing (D3)

Any two criteria stated Sample answer


Exhaled air contains carbon dioxide (D1) affected by different level of the floor stair climbing (D3)

Any one criteria stated 1.Percentage of carbon dioxide is affected by the stair climbing(D3) 2. Exhaled air contains carbon dioxide (D1) None of the above or no response 156 1 0

(c) (ii)

KB0602 - Classifying Able to classify the apparatus and material used in this experiment correctly Sample Answer Material Potassium hydroxide solution water student 6 Ticks 5 Ticks 3- 4 Ticks No response or wrong response Apparatus J-tube Thermometer stair 3

2 1 0

157

Question 2 Our body gains and loss water every day. Maintaining the water balance of body fluids depends on maintaining the blood volume and blood osmotic pressure. The process of maintaining the water content of the blood at a constant level is called osmoregulation. Osmoregulation is achieved mainly by regulating the volume of urine production from the kidneys. Design a laboratory experiment to study the effect of different volume of water intake on volume of urine output. The planning of your experiment must include the following aspects: Problem statement Aim of investigation Hypothesis Variables List of apparatus and materials Technique used Experimental procedures or methods Presentation of data Conclusion

[17 marks]

158

QUESTION 2 PROBLEM STATEMENT No. Mark Scheme Score 3

Able to state a problem statement relating the manipulated variable 2(i) KB061201 with the responding variable correctly P1 : volume of water intake P2 : Volume of urine output H : question form and question mark(?)
1. What is the effect of volume of water intake on the volume of urine output? 2. How does volume of water affect the volume of urine output?

Sample answer

Able to state a problem statement less accurately Sample answer 1. What is the effect of volume of water intake on the volume of urine output. (no H) 2. What is the effect of volume of water intake on the urine output? (incomplete P2) 3. What is the effect of water intake on the volume of urine? (incomplete P1) Able to state a problem statement at idea level Sample answer 1. What is the volume of urine output. (no P1 and H) 2. What is the effect of volume of water intake.(no P2 and H) No response or incorrect response

159

AIM OF INVESTIGATION No. Mark Scheme Able to state the objective of the investigation correctly 2 (ii) KB061203 Sample answer To study the effect of different volume of water intake on volume of urine output. Score

HYPOTHESIS No. Mark Scheme Able to state a hypothesis relating the manipulated variable to the 2 (iii) KB061202 responding variable correctly P1 : volume of water intake P2 : volume of urine output H : relationship Sample answer 1. The higher the volume of water intake, the higher the volume of urine output. Able to state a hypothesis inaccurately *Have P1 and P2 or P1/P2 and H Sample answer 1. The volume of urine output is affected/influences by the volume of water intake. (no H) Able to state a hypothesis at idea level Sample answer 1. Water intake affects the volume of urine output (only P2) 2. If more water is taken, more urine will be released . No response or incorrect response 0 1 2 Score 3

160

VARIABLES No. 2 (iv) KB061203 Mark Scheme Able to state all three variables correctly Sample answer Manipulated : Volume of water intake Responding : Volume of urine output Fixed : Type of drink / time to collect urine / same student Score

LIST OF APPARATUS AND MATERIALS No. Mark Scheme Able to list all the important apparatus and materials correctly 2(v) KB061205 Sample answer Apparatus : drinking cups, container for collecting urine, and measuring cyllinder Materials : distilled water, 3 apparatus + 1 materials Able to list any 1 material and 2 apparatus Able to list any 1 material and 1 apparatus No response or incorrect response Remark : At least 1 score to get for title materials and apparatus for planning experiment.. 2 1 0 Score 3

161

TECHNIQUE USED No. Mark Scheme Able to state the operating responding variable correctly using 2 (iv) KB061203 suitable apparatus Sample answer 1. Measure and record the volume of urine output using a measuring cylinder Score B1 = 1

PROCEDURE No. 2 (vii) KB061204 Mark Scheme Able to describe the steps of experiment correctly based on the following aspects: K1 : Preparation of materials and apparatus (any 3) K2 : Operating the constant variable (any 1) K3 : Operating the responding variable (any 1) K4 : Operating the manipulated variable (any 1) K5 : Steps to increase reliability of results accurately (any 1) Sample answers 1. The night before the experiment, the participating student is not to consume any food or drinks after 10.00pm. 2. A student empties his or her urinary bladder at the beginning of the experiment. 3. Measure 200ml of distilled water (K2) and put it into the mug/cup 4. A student is given 200ml of distilled water to drink 5. A stop watch is started immediately after consuming the water. 6. He is instructed not to eat or perform any vigorous physical Activities (within the given time) 7. After two hours he is asked to empty his urinary bladder. The volume of urine output is measured using measuring cylinder and recorded. 8. The same student (K2) repeat step 1 7 for different amount of distilled water on different day. * Accept four readings and more Day 2 Day 3 Day 4 Day 5 400 ml 600 ml 800 ml 1000 ml Score

162

K1 ( All 4) 1. Measure volume of distilled water (water intake) 2. Put in cup/mug 3. Drink distilled water 4. Empty his urinary bladder K2 (any 1) 1. Fix the volume of distilled water 2. Fix the student K3 1. Record volume of urine output K4 1. Repeat the experiment with another volume of distilled water K5 (any 1) 1. Student is not to consume any food or drinks after 10.00pm. 2. Empties his or her urinary bladder at the beginning of the experiment. 3. Not to eat or perform any vigorous physical activities (within the given time) All 5 'K' Any 3 - 4 K Any 2 K No response or incorrect response or 1 K only. Remark: 1K - for title procedure for planning experiment. 3 2 1 0

163

No. 2 (viii) KB061203

Mark Scheme Able to construct a table to record data based on the following aspects : 1. Correct title and units (*titles compulsary) Sample answer Day 1 2 3 4 5 * Volume of water intake ,ml 200 400 600 800 1000 * Volume of urine output, ml

Score B2 = 1

CONCLUSION No. 2(ix) KB061203 Mark Scheme Able to make the right conclusion correctly Sample answer The higher the volume of water intake, the higher the volume of urine output. Hypothesis is accepted Reject conclusion if students only state Hypothesis is accepted Score

PLANNING OF EXPERIMENT No. Mark Scheme Able to write the experimental planning 2(x) KB061203 1. Problem Statement 2. Aim 3. Hypothesis 4. Variables 5. Materials and Apparatus 6. Technique 7. Procedure 8. Result 9. Conclusion 164 Score

7 - 9 ticks 4 - 6 ticks 2 - 3 ticks 0 1 tick No response or incorrect response

3 2 1 0

END OF MARKING SCHEME

165

MARKING SKILL PAPER 3 (EXERCELANCE STUDENTS ANSWER) 1. A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis. Diagram 1 shows the apparatus set-up to collect the gas produced when the aquatic plant received different intensities of light. The aquatic plant will illuminated from a lighted bulb of 40 watts for periods of 5 minutes at five different distance, d, from the bulb. At the end of each 5 minutes periods, a gas bubble was drawn into the capillary tube using syringe. The length of the gas bubble was measured using a ruler and recorded. The temperature in the water bath was maintained at 25OC throughout this experiment.

Diagram 1

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PAPER 3 (MODERATE STUDENTS ANSWER) 1. A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis. Diagram 1 shows the apparatus set-up to collect the gas produced when the aquatic plant received different intensities of light. The aquatic plant will illuminated from a lighted bulb of 40 watts for periods of 5 minutes at five different distance, d, from the bulb. At the end of each 5 minutes periods, a gas bubble was drawn into the capillary tube using syringe. The length of the gas bubble was measured using a ruler and recorded. The temperature in the water bath was maintained at 25OC throughout this experiment.

Diagram 1

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