DEHYDRATION

Jenivie Morillo Jose University of Santo Tomas National Childrens Hospital

 process of removing EXTRACELLULAR and

INTRACELLULAR water from tissue

IDEAL CHARACTERISTICS:
dehydrate tissue rapidly without SHRINKAGE

not EVAPORATE very fast

able to DEHYDRATE even FATTY tissues not HARDEN the tissue excessively not be TOXIC to the handler

THREE COMMONLY SOLUTIONS: alcohol, acetone, dioxane

ALCOHOL METHOD -use of ETHYL ALCOHOL, METHYL ALCOHOL, BUTYL and ISOPROPYL ALCOHOL are recommended for routine dehydration of tissues -BUTYL and ISOPROPYL ALCOHOL is SLOW DEHYDRATING AGENT -consists of passing the tissue through a series of progressively more concentrated alcohol baths -the more DELICATE the tissue, the LOWER is the grade of alcohol and SMALLER the intervals should be between the strengths of the ascending alcohols

ACETONE METHOD
-for MOST urgent biopsies -UNSUITABLE for routine work

DIOXANE METHOD (Diethylene Dioxide)

-UNIQUE reagent(miscible with both water and

molten paraffin wax) -should be used only in A WELL VENTILATED LABORATORY -EXCELLENT DEHYDRATING and CLEARING AGENT

TETRAHYDROFURAN
-used as BOTH DEHYDRATING and CLEARING

AGENT (miscible in both water and paraffin) -dissolve FATS -miscible in LOWER ALCOHOLS, ETHER, CHLOROFORM, ACETONE, BENZENE and XYLENE -doesnt dissolve out ANILINE DYES -MOST STAINING procedure gives improved results

CELLOSOLVE(Ethylene Glycol Monoethyether)

-with RAPID ACTION without OVERHARDENING

and DISTORTION of TISSUES

Tri-ethyl Phosphate
-removes water very rapidly -produces very little DISTORTION -soluble in ALCOHOL, ETHER, BENZENE,

CHOLOFORM, ACETONE and XYLENE -produces MINIMUM tissue shrinkage

Thank you