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The document provides instructions for performing a stool culture to identify various bacterial pathogens that may be present in a stool sample. It lists the different selective media that should be used, such as MacConkey agar, XLD agar, EMB agar, TCBS agar, and campylobacter selective media. The document also provides information to help identify common stool pathogens like E. coli, Salmonella, Shigella, Campylobacter, and Vibrio cholerae based on their growth characteristics on different culture media.
The document provides instructions for performing a stool culture to identify various bacterial pathogens that may be present in a stool sample. It lists the different selective media that should be used, such as MacConkey agar, XLD agar, EMB agar, TCBS agar, and campylobacter selective media. The document also provides information to help identify common stool pathogens like E. coli, Salmonella, Shigella, Campylobacter, and Vibrio cholerae based on their growth characteristics on different culture media.
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The document provides instructions for performing a stool culture to identify various bacterial pathogens that may be present in a stool sample. It lists the different selective media that should be used, such as MacConkey agar, XLD agar, EMB agar, TCBS agar, and campylobacter selective media. The document also provides information to help identify common stool pathogens like E. coli, Salmonella, Shigella, Campylobacter, and Vibrio cholerae based on their growth characteristics on different culture media.
Copyright:
Attribution Non-Commercial (BY-NC)
Verfügbare Formate
Als PPT, PDF, TXT herunterladen oder online auf Scribd lesen
وعين الصقر ميزانة و سبحان من ل يخطأ Stool or Rectal swab culture *macConky plate (NLF) *XLD agar (shigila→???/ salmonila→black) *EMB agar → E - mythylen blue *HE *TCBS (y.colores) → vribo & cholera → yellow color *alkaline peptone water (APW) → cholera *selenite F broth *selective media for campylobactor (skirrow &blaser with suplements) campylobactor microaerophilic (42C-O2 5%- Co210%-N2 85%-48hr) selenite F broth
For salmonila suspectef organisms
1- E-coli (infant &NLF) it not a normal flora in
infant
2- salmonella & shigella
3-campylobactor jejuni most common human pathogen indol&citrate (+) urease (- ) oxidase (+) nalidixic acid (S) s= sensitive
cephalo thin (R) r/
campylobactor 4-V.cholerae serogroup -01 catalse (+) oxidase (+) motile (+) lactose (NLF) sensitive to (dry – sunlight – acid pH) TCBS (thiosulfate citrate bile sucrose agar –yallow colonies) Vibrio cholerae TCBS The End