Beruflich Dokumente
Kultur Dokumente
Surface Modifications,
Microvilli
Three Types of
Junctions
Occluding junctions – Zonula
occludens
restrict and direct movement of fluids
in intercellular space
Focal adhesions in a band around cell;
composed of
Transmembrane = occludin,
cytoplasmic proteins = ZO 1-3 (1 is
tumor supressor, 2 is part of EGF
signaling, 3 is linker)
Breach ZO – leaky epithelia
Most apical attachment, restricting
movements of PM proteins and
maintaining integrity of apical vs.
basal/lateral surfaces
Tightness of anastomosing network
differs b/w tissues
Three Types of
Junctions, cont’d
Anchoring Junctions (lateral face)
Zonula Adherens
• Lateral adhesion
• Continuous band of transmembrane
cadherins bound to catenin/vinculin/actin
on cytoplasmic side
• Adhesion is Ca+ dependent
Macula Adherens (Desmosomes)
• High tensile strength
• Desmoplakin/plakoglobin attach to
intermediate filaments
• Not a continuous structure around cell
• Attachment plaque – shock absorber
• Attach to other cells by desmogleins
(cadherin zipper)
Three Types of
Junctions, cont’d
Communicating Junctions
Gap junctions
Lateral pores composed of
connexins
Pore size alters, but still restricts
cell-cell communication
physically
Lowers electrical resistance in
cells (permits ion passage)
Protein = Connexin
Basal Face
Basement Membrane
Basal lamina
• Collagen
• Proteoglycans
• Laminin
• Entactin and Fibronectin
• H & E stains poorly; use PAS
• Beneath Basal Lamina is Reticular lamin
(connective tissue)
• Attachment, Compartmentalization,
Filtration, Polarity induction, Tissue
scaffolding
Cell-ECM junctions
Focal Adhesions (via actin)
Hemidesmosomes (present in
mechanically abraded tissues)
PM foldings
Glands
Exocrine vs Endocrine – How are they different?
Merocrine
Vesicle bound products;
exocytosis
Apocrine
Released in apical portion of cell
Holocrine
Apoptosis related release (eg.,
sebaceous glands)