DEPARTMENT OF TECHNICAL EDUCATION

ANDHRA PRADESH
Name : A. Vinay Kumar
Designation : H.O. D
Branch : Applied Electronics and Instrumentation
Institute : Q.Q.GPT , HYD
Year/semester : VI Sem
Subject : Analytical Instrumentation
Code No : AEI-603
Major Topic : Mass spectroscopy & Chromatography
Duration : 50Mts
Sub Topic : Operation of Liquid chromatography
Teaching Aids : Animations, Diagrams

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 Objectives

On completion of this topic you would be able

to know about Liquid Chromatography

• Operation

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 Recap

In last class we have studied about Liquid

chromatograph
• Introduction

• Principle

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Liquid chromatograph

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Block diagram of Liquid chromatograph

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Liquid chromatograph

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Liquid chromatography consists of

• High pressure Pump system

• Gradient elution or solvent programming

• Sample injection system

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• The column

• The detector system

• Display

• Recording device

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 Operation

• Sample is introduced into the column with the help of a

sample injection system

• Various compounds of the sample are fractioned during

their passage through the column

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 Operation…..

• The detection system senses these components as they

elute from the column

• It produces a signal proportional to the amount of solutes

passing through the system

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Liquid Chromatography

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Liquid Chromatography

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 Summary

In this class we have learnt about Liquid

Chromatography
• Operation

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 Quiz
1. In Liquid Chromatography Sample is injected
at ----- stage
a) Pump

b) Column

c) Detector

d) None

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 Frequently Asked Questions

1.Explain the operation of Liquid chromatography?

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