Candy Chromatography: What Makes Those

Colors?

Abstract
Quick, what's your Iavorite color oI M&Ms candy? Do you want to know what dyes were used
to make that color? Check out this project to Iind out how you can do some scientiIic detective
work to Iind out Ior yourselI.
Objective
The goal oI this project is to use paper chromatography to see which dyes are used in the
coatings oI your Iavorite colored candies.
Introduction
Have you ever had a drop oI water spoil your nice print-out Irom an inkjet printer? Once the
water hits the paper, the ink starts to run. The water is absorbed into the Iibers oI the paper by
capillary action. As the water travels through the paper, it picks up ink particles and carries them
along. This same process that spoils a perIect print-out can also be put to good use. There's even
a name Ior it: paper chromatography.
Chromatography is a technique used to separate the various components in a complex mixture or
solution. It works because the components oI the mixture will diIIer in how much they "stick" to
things: to each other, and to other substances. For example, some oI the components oI the ink
will stick more tightly to the paper Iibers. They will spend less time in the water as it moves
along the paper Iibers, and thus they will not travel very Iar. Other components oI the ink will
stick less tightly to the paper Iibers. They will spend more time in the water as it moves along the
paper Iibers, and thus they will travel Iarther through the paper.
Other materials than paper and water can be used Ior chromatography, but in each
chromatography apparatus there is generally a stationary phase and a mobile phase. In paper
chromatography, the paper is the stationary phase, and water is the mobile phase. Another
example oI a chromatography systems is a glass column Iilled with tiny, inert beads (the
stationary phase). The mixture to be separated is added to the column, and is then "washed out"
with some type oI solution (the mobile phase). In this case, the separation is based on molecular
size. Smaller molecules will pass through the spaces between the beads more easily, so they will
come out oI the column more quickly. Larger molecules will take more time to pass between the
beads, so they will come out oI the column later. You can separate the smaller molecules Irom
the larger molecules by collecting the liquid that comes oII such a column in a series oI separate
containers.
Chromatography can be used to separate (puriIy) speciIic components Irom a complex mixture,
based on molecular size or other chemical properties. It can also be used to identiIy chemicals,
Ior example crime scene samples like blood, drugs, or explosive residue. Highly accurate
chromatographic methods are used Ior process monitoring, Ior example to assure that a
pharmaceutical manuIacturing process is producing the desired drug compound in pure Iorm.
With colored mixtures in paper chromatography, you can see the components separate out on the
paper.
To measure how Iar each component travels, we calculate the retention Iactor (R
I
value) oI the
sample. The R
I
value is the ratio between how Iar the component travels and the distance the
solvent travels Irom a common starting point (the origin). II one oI the sample components
moves 2.5 cm up the paper and the solvent moves 5.0 cm, then the R
I
value is 0.5. You can use
R
I
values to identiIy diIIerent components as long as the solvent, temperature, pH, and type oI
paper remain the same. In the image below, the light blue shading represents the solvent and the
dark blue spot is the chemical sample.

When measuring the distance the sample traveled, you should measure Irom the origin (where
the middle oI the spot originally was) and then to the center oI the spot in its new location.
To calculate the R
I
value, we use the equation:
R
I

distance traveled by the sample component
distance traveled by the solvent
In our example, this would be:
R
I

2.5 cm
5.0 cm
0.5
ote that an R
I
value has no units because the units oI distance cancel.
In this project, you'll use the R
I
value to compare the "unknown" components oI colored candy
dyes with the "known" components oI Iood coloring dyes. Since there are only a small number
oI approved Iood dyes, you should be able to identiIy the ones used in the candies by comparison
to the chromatography results Ior Iood coloring.
Terms, Concepts, and Questions to Start Background Research
To do this project, you should do research that enables you to understand the Iollowing terms
and concepts:
O Adhesion, cohesion Iorces
O Capillary action
O !aper chromatography
O Stationary phase
O Mobile phase
O Hydrophilic
O Hydrophobic
O Retention Iactor (R
I
)
O Solvent
O Solution
Questions
O Why do diIIerent compounds travel diIIerent distances on the piece oI paper?
O How is an RI value useIul?
O What is chromatography used Ior?
Bibliography
O This website is a good reIerence Ior basic chemistry concepts:
Andrew Rader Studios, 19972007. "Chem4Kids," Chem4Kids.com |accessed July 17,
2007| http://www.chem4kids.com.
O Here you'll Iind an overview oI liquids and capillary action:
MicrosoIt Corporation, 20032007. "Liquids," MicrosoIt Encarta |accessed July 17,
2007|
http://encarta.msn.com/encnet/reIpages/ReIArticle.aspx?reIid761571486&sec18#s18.
O This is a good general reIerence on chromatography, written by a high school sophomore
in an A! Chemistry course:
VanBlaricum, A., 1997. "Chromatography," |accessed July 17, 2007|
http://www.doggedresearch.com/chromo/chromatography.htm.
O This project idea is based on:
Helmenstine, A.M., 2007. "Candy & CoIIee Filter Chromatography,"
About.com:Chemistry |accessed July 17, 2007|
http://chemistry.about.com/od/chemistryexperiments/ht/candychroma.htm.
Materials and Equipment
To do this experiment you will need the Iollowing materials and equipment:
O Candy with a colored coating, like Skittles or M&Ms
O At least 30 strips oI paper
4 All strips must be exactly the same size.
4 3 cm 9 cm is a good size, but you can change this to Iit your needs.
4 You can use white cone-type coIIee Iilters cut into strips, or you can use
chromatography paper.
O Wide-mouth jar
O !encil
O Ruler
O Tape
O Salt
O Water
O Toothpicks
O Food coloring (red, green, and blue)
Experimental !rocedure
1. Do your background research so that you are knowledgeable about the terms, concepts,
and questions, above.
2. Use a pencil to lightly label which candy color or Iood coloring will be spotted on each
paper strip. Tip: do not use a pen Ior writing on the strips: the ink will run when the
solvent passes through the strips.
3. Draw a pencil line 2 cm Irom the edge oI each strip oI paper.
a. This will be the origin line.
b. You will spot the candy color Ior each strip right on this line, as shown below.

4. ext you need to extract some dye Irom each candy you wish to test.
a. Set the candy down on a clean plate in a single drop oI water. Note. II you use too
much water, the dye will not be concentrated enough to see on the
chromatography paper.
b. Leave it Ior a minute to allow the dye to dissolve.
c. Remove the candy, then dip a clean toothpick into the now-colored drop oI water.
d. Spot the candy dye solution onto the chromatography paper by touching the
toothpick to the chromatography strip, right in the center oI the origin line.
e. Allow the spot to dry, then repeat the spotting at least three more times. You want
to make sure to have enough dye on the chromatography paper so that you can see
the dye components when they separate out on the paper.
I. Make Iive separate strips Ior each candy you want to test.
Repeat step 4 Ior each color oI candy you want to test (at least three diIIerent colors).
You also need to prepare chromatography strips with Iood coloring dyes.
. These will be your known compounds, with which you will compare the
"unknown" candy dyes.
a. For each Iood coloring color, use the same procedure as in step 4. You'll use a
drop oI Iood coloring as the source Ior dipping your clean toothpick.
!repare a 0.1 salt solution Ior the chromatography solvent.
. Add 1/8 teaspoon oI salt to 3 cups oI water (1 g oI salt to 1 L oI water).
a. Shake or stir until the salt is completely dissolved.
!our a small amount oI the salt solution into the wide-mouth jar.
. You'll tape the strip to a pencil and rest the pencil on top oI the jar so that the strip
hangs into the jar.
a. The goal is to have the end oI the chromatography strip just touching the surIace
oI the solvent solution, as shown in the drawing below.

Let the solvent rise up the strip (by capillary action) until it is almost at the top, then
remove the strip Irom the solvent. Keep a close eye on your strip and the solvent IrontiI you
let it run too long the dye may run oII the paper and become distorted.
Use a pencil to mark how Iar the solvent rose with a pencil.
Allow the strip to dry, then measure the R
I
value Ior each candy color (or Iood coloring)
dye component.
Using the Iive repeated strips Ior each candy color (or Iood coloring), calculate the
average R
I
Ior each dye component.
Compare the R
I
values Ior the candy colors and the Iood coloring dyes. Can you identiIy
which Iood coloring dyes match which candy colors?
Variations
O For a chromatography experiment on separating the ink components Irom markers, see
the Science Buddies project !aper Chromatography: Basic Version.
O Try this project with a variety oI candiesIor example, does the red in Skittles look the
same as the red in M&Ms when processed with chromatography? Is the average RI
value nearly the same? Look in the ingredients on each package to try and determine iI
the same dyes were used.
O For more advanced chromatography experiments, see the Science Buddies projects
4 !aper Chromatography: Advanced Version 1, and
4 !aper Chromatography: Advanced Version 2.
O For more science project ideas in this area oI science, see Cooking & Food Science
!roject Ideas.
Credits
Andrew Olson, !h.D., Science Buddies
Sources
This project idea is based on:
O Helmenstine, A.M., 2007. "Candy & CoIIee Filter Chromatography,"
About.com:Chemistry |accessed July 17, 2007|
http://chemistry.about.com/od/chemistryexperiments/ht/candychroma.htm.