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Substances A and B have retention times of 16.40 and 17.63 min, respectively, on a 30.0 cm column. An unretained species passes through the column in 1.30 min. The peak widths (at base) for A and B are 1.11 and 1.21 min, respectively. Calculate 1. the column resolution 2. the average number of plates in the column 3. the plate height 1. Rs= 2(t R2 t R1) W A+ W B
2(17.6316.40) = 1.06 1.11+ 1.21
Rs=
2. tR 2 N =16 ( ) W N =16 ( 16.40 ) =3493 1.11 17.63 ) =3397 1.21 3493+ 3397 )=3445 2
2 2
N =16 (
N AVG =( 3.
H=
Skoog, West et al, Fundamentals of analytical chemistry, 8th ed, ISBN-13: 978-0-03-035523-3