Development and characterisation of gelatin-based nanoparticles for targeted delivery of Zidovudine

Preeti Irny*, Ratan S. Tone, Namdeo R. Jadhav Department if pharmaceutics,Bharati vidyapeeth college of pharmacy, Near Chitranagari, Kolhapur 416013, India. Email: preetie_88@yahoo.co.in Abstract The present investigation was aimed at developing and exploring the use of mannosylated gelatin nanoparticles for the selective delivery of an anti-HIV drug, Zidovudine. Zidovudine (AZT) is an antiretroviral agent used in treatment of AIDS having low biological half life (0.5-3hrs) with numerous dose dependant side effects and thus it is good candidate for the formulation of sustained release dosage forms.The mannosylated gelatin nanoparticles (MN-G-NPs) were prepared using a two-step desolvation technique and coupled with mannose using the amino group of gelatin present on the surface of nanoparticles. The mannosylation was confirmed using infrared spectroscopy. MN-G-NPs were characterized for shape, particle size, zeta potential, and percentage drug entrapment. The size of nanoparticles was found to be in range of 200-800 nm. In-Vitro drug release studies showed release up to 79.98% to 88.04% up to 18 hrs .Coupling of the nanoparticles with mannose significantly enhances the lung, liver, and lymph nodes uptake of drug, could possibility be advantageous in reducing viral load with efficacy. increased therapeutic

Keywords: Zidovudine, Targeted delivery, Macrophages