Diluio

pH
Amostras
Tempo (min)
0.25
0.5
0.75
1
1.25
1.5
1.75
2
2.25

AB -01
0.48
1.09
1.71
-

20x
pH 3,0
AB -02
0.16
0.4
0.69
0.97
1.18
1.29
1.36
1.4
1.41

AB -03
0.26
0.54
0.86
1.11
1.25
1.33
1.36
1.37
1.37

AB -01
0.08
0.17
0.27
0.42
0.59
0.78
0.97
1.16
1.32

20x
pH 4,0
AB -02
0.04
0.1
0.18
0.28
0.38
0.51
0.65
0.79
0.93

Espcie: Agaricus blazei

4x
pH 5,0
AB -03
AB -01
AB -02
0.19
0.35
0.53
0.72
0.92
1.12
1.32
1.57
1.67

0.11
0.39
0.54
0.75
0.97
1.21
1.44
1.68
1.9

0.13
0.22
0.31
0.42
0.53
0.67
0.81
0.95
1.1

Agaricus blazei

AB -03
0.31
0.43
0.57
0.73
0.87
1.01
1.26
1.35
1.4

2x
pH 6,0
AB -01
AB -02
0.11
0.13
0.14
0.15
0.16
0.17
0.18
0.2
0.21

0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.17

AB -03
0.28
0.28
0.29
0.29
0.29
0.29
0.29
0.29
0.29

AB -01
0.25
0.27
0.26
0.26
0.25
0.25
0.25
0.26
0.26

pH 7,0
AB -02
0.36
0.36
0.35
0.35
0.34
0.34
0.34
0.34
0.34

AB -03
0.5
0.49
0.49
0.49
0.49
0.49
0.49
0.49
0.49

AB 2,0 PH 3,0

y = 0.9451x - 0.0453
R = 0.9838
1.5
ABSORBNCIA

Tempo (min) AB 2,0 PH 3,0

0.25
0.16
0.5
0.4
0.75
0.69
1
0.97
1.25
1.18
1.5
1.29

AB 2,0 PH 3,0

0.5
0
0

Linear (AB 2,0

PH 3,0)

TEMPO (MIN)

AB 2 PH 5
Axis Title

Tempo (min) AB 2 PH 5
0.25
0.13
0.5
0.22
0.75
0.31
1
0.42
1.25
0.53
1.5
0.67
1.75
0.81
2
0.95
2.25
1.1

1.2
1 y = 0.488x - 0.0389
R = 0.9915
0.8
0.6
0.4
0.2
0
0
1
2
Axis Title

AB 2 PH 5
Linear (AB 2 PH
5)
3

AB 2, PH 4,0

Axis Title

Tempo (min) AB 2,0 PH 4,0

0.25
0.04
0.5
0.1
0.75
0.18
1
0.28
1.25
0.38
1.5
0.51
1.75
0.65
2
0.79
2.25
0.93

1
0.8y = 0.4533x - 0.1378
R = 0.9844
0.6
0.4

Series1

0.2

Linear (Series1)

0
-0.2 0

1
2
Axis Title

Tempo (min) AB 3,0 PH 3,0

0.25
0.26
0.5
0.54
0.75
0.86
1
1.11
1.25
1.25

ABSORBANCIA

AB 3,0 PH 3,0
1.4
1.2
1
0.8
0.6
0.4
0.2
0

y = 1.02x + 0.039
R = 0.9833
AB 3,0 PH 3,0
Linear (AB 3,0 PH
3,0)
0

0.5

1.5

TEMPO (MIN)

AB 3 PH 5,0
2
Axis Title

Tempo (min) AB 3 PH 5,0

0.25
0.31
0.5
0.43
0.75
0.57
1
0.73
1.25
0.87
1.5
1.01
1.75
1.26
2
1.35
2.25
1.4

y = 0.5853x + 0.1494
R = 0.9893

1.5

AB 3 PH 5,0

1
0.5

Linear (AB 3 PH
5,0)

0
0

2
Axis Title

near (AB 3,0 PH

AB 03 PH 4,0
y = 0.7707x - 0.0311
R = 0.9972

2
Axis Title

B 3,0 PH 3,0

Tempo (min) AB 03 PH 4,0

0.25
0.19
0.5
0.35
0.75
0.53
1
0.72
1.25
0.92
1.5
1.12
1.75
1.32
2
1.57
2.25
1.67

1.5

AB 03 PH 4,0

1
0.5
0
0

Axis Title

Linear (AB 03
PH 4,0)

3
4
5
6
7

AB 1
AB 2
AB 3
6833.4 13663.8 20494.2
1780.6
3557.2
5333.8
492.2
979.4
1466.6
13.06
20.12
27.18
0
0
0

25000
Ativ. Enzimtica Lacase (UI/mL)

PH

Atividade Laca

20000
15000
10000
5000
0
3

Atividade Lacase x pH

AB 1
AB 2
AB 3

6
ph