Effect of Japanese radish (Raphanus sativus) sprout (Kaiware-daikon) on carbohydrate and lipid metabolisms in normal and streptozotocin-induced diabetic

rats
1. 2. 3. 4. 5. 6. 7. 8. Hironobu Taniguchi1, Kazuo Kobayashi-Hattori2,*, Chie Tenmyo2, Tomoko Kamei2, Yasushi Uda3, Yoshiko Sugita-Konishi4, Yuichi Oishi5, Toshichika Takita2 Article first published online: 24 MAR 2006

No information is available about the effects of Japanese radish sprout (JRS) on diabetes. To clarify the effects, the influence of JRS on carbohydrate and lipid metabolisms was investigated in normal and streptozotocin-induced diabetic rats. The rats were fed a diet containing 0%, 2.5% or 5% of JRS ad libitum for 21 days. Compared with the corresponding control groups, the JRS-fed normal rats showed lower plasma levels of total cholesterol (TC), triglycerides (TG), phospholipids (PL), fructosamine, glucose and insulin and higher plasma levels of low-density lipoprotein-cholesterol, whereas the JRS-fed diabetic rats showed lower plasma levels of fructosamine, glucose and insulin without changes in the plasma lipid parameters. JRS also decreased the hepatic TC, TG and PL levels in the normal rats and the TG level in the diabetic rats. These results showed that JRS had a hypoglycemic activity in both the normal and diabetic rats and partly improved lipid metabolism in the normal rats. JRS has the potential to alleviate hyperglycemia in cases where diabetes is present and to serve in the primary prevention of diabetes mellitus. Copyright 2006 John Wiley & Sons, Ltd.

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Journal of Nutritional Science and Vitaminology Vol. 53 (2007) No. 3 P 261-266

Differing Effects of Water-Soluble and Fat-Soluble Extracts from Japanese Radish (Raphanus sativus) Sprouts on Carbohydrate and Lipid Metabolism in Normal and StreptozotocinInduced Diabetic Rats
Hironobu TANIGUCHI1), Rieko MUROI2), Kazuo KOBAYASHI-HATTORI2), Yasushi UDA3), Yuichi OISHI2), Toshichika TAKITA2)

We have shown previously that Japanese radish (Raphanus sativus) sprouts (JRS) improve blood glucose levels in diabetic rats. In this study, we investigated the components in JRS that caused this hypoglycemic effect, by examining the effects of water-soluble (WSE) and fat-soluble (FSE) extracts of JRS on diabetes markers in normal (NM) and streptozotocin (STZ)-induced diabetic (DM) rats. The NM and DM rats were divided into a control group and 2 test groups (WSE

(2.2%) or FSE (0.2%)), with the rats (n=6/group) then being maintained for 3 wk on either a control diet or one of the test diets; this was followed by the measurement of serum concentrations of glucose, insulin, glycoalbumin, fructosamine, ketone bodies, and lipids (cholesterol and triglyceride) and liver concentrations of lipids (total lipid, total cholesterol, and triglyceride). The FSE suppressed insulin secretion and improved lipid metabolism in the NM rats. The effect of WSE was different from that of the FSE as it decreased blood glucose levels without increasing insulin secretion and also lowered glycoalbumin and fructosamine levels in the DM rats. Therefore, the WSE have potential as functional food components with the hypoglycemic effect.

Antidiabetic effect of Raphanus sativus root juice

Authors: Shukla, Surekha; Chatterji, Sanjukta; Mehta, Shikha; Rai, Prashant Kumar; Singh, Rakesh Kumar; Yadav, Deepak Kumar; Watal, Geeta Source: Pharmaceutical Biology (Formerly International Journal of Pharmacognosy), Volume 49, Number 1, January 2011 , pp. 32-37(6)

Abstract: Context: Many plants have been explored scientifically and systematically and claimed to be useful for the treatment of diabetes mellitus by various research groups worldwide. The present study is a further effort in the direction of developing a novel oral antidiabetic agent of high potential with minimal or no side effects. Objective: This study screened the glycemic attributes of Raphanus sativus L. (Brassicaceae) root juice in normal as well as sub- and mild-diabetic models. 1 Materials and methods: The variable doses of 100, 200, 300, and 400mgkg body weight (bw) of the extract were administered orally to normal and streptozotocin (STZ)-induced sub- and mild-diabetic rats in order to define its glycemic potential. Glibenclamide was used as a reference drug. 1 Results: The dose of 300mgkg bw was identified as the most effective dose which lowers the blood glucose level (BGL) by 33.4% (p<0.001) at 6h during fasting blood glucose (FBG) studies in normal rats. However, the glucose tolerance test (GTT) revealed the maximum reduction of 15.9% (p<0.001) in BGL at 3h in normal rats with the same dose, whereas the reduction observed was by 23.8 and 28.3% (p<0.001) in sub- and mild-diabetic rats, respectively, at the same interval of time. Discussion and conclusion: This evidence clearly indicates that Raphanus sativus root juice possesses good hypoglycemic potential coupled with antidiabetic efficacy.