KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCESUNIVERSITY,NANDINAGAR CAMPUS, BIDAR-5845401 LABORATORY MANUAL For: VETERINARY NEURO PHARMACOLOGY [VPT

321] DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGYVETERINARY COLLEGE (BIDAR), P.BNO.6; PIN: 585 401DEPARTMENT OF VETERINARY PHARMACOLOGY AND TOXICOLOGY 1

pharmacology of invitro tissue preparation. This isolated tissue usedfor experiments have several advantages over intact animals asrelatively small amount of the test material is required and severalpreparation can be tested from a single animal. Intestine (ilium),c o l o n , t r a c h e a l r i n g s , a r t e r i a l r i n g s , c e r v i c a l s t r i p s , v a s deferens,diaphragm, oesophagus, smooth and skeletal muscle andrectus ab dominus muscle from different laboratory animals are themost common isolated tissues used for the experimental studies . Organ bath: T h e t i s s u e b a t h u s e d t o p u t t h e a n i m a l t i s s u e f o r studying the drug actions in called students organ bath. The organbath having two units of inner tissue bath is called double unit organb a t h . T h i s w a s f i r s t d e s i g n e d b y R u d o l p h M a g n u s i s 1 9 0 4 a n d i t esse ntially consists of following parts. Water bath or outer jacket: I t i s m a d e u p o f s t e e l , g l a s s o r perspex (transparent thermoplastic resi n). It holds water andother parts of organ bath. Inner organ or tissue bath: It is cylindrical glass with variable capacity. It contains isolated tissue in physiological salt solution. Heating rod or electrical heater with thermostatic control: Itheats the water in outer jacket to a desired temperature Stirrer: A small electrical stirrer keeps the water in outer jacket circulating at uniform temperature Glass coil or condenser:

A c o i l m a d e o f g l a s s o r p e r s p e x i s connected at one end to the lower end of the organ bath andother to the container having the physiologic salt solution. Itusuall y has double the capacit y of inner organ bath to ensure warming of the solution before it enters the organ bath Oxygen delivery tube: I t i s m a d e u p o f a h o l l o w g l a s s t u b e . I t delivers oxygen to the tissue and also serves as tissue holder.L o w e r e n d o f t h e t i s s u e i s t i e d w i t h a t h r e a d t o i t s S - s h a p e d curved end. The lower end has a small hole through which air oroxygen is supplied to ensure proper aeration of the tissue Aerator: It is a small electrically operated device, which is connectedto the oxygen delivery tube through rubber tubing. It dispenses oxygen( or carbogen: 5%CO and 95%O) to the tissue in the inner tissue bath. The purpose of aeration is supply of oxygen, maintenance of pH anduniform distribution of drug due to bubbling. Writing /Recording levers: They are used to record the contractionor relaxation of the isolated tissue preparation. The recording is done 4