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BIOORGANIC CHEMISTRY BioOrganic Chemistry Laboratory CH 205 (2011-2012) Experiment 7

Analysis of Lipids
Lejla Angela Tamara R. Biscocho, Pia Angela J. Blanco, Honey Joy C. Capiral, Carmela Marie A. Carandang Department of Speech-Language Pathology, College of Rehabilitation sciences University Of Santo Tomas, Espana Street, Manila 1008
Date Submitted: February 28, 2012 Abstract: Keywords: 1. 2. 3. Introduction Methodology Results and Discussion Table 1: Reactions of the standards in different tests LiebermannSalkowski Burchard Test Test Lecithin Cerebroside Cholesterol Emerald green solution 3 layers (colors) -red solution - turbid yellow solution -clear yellow solution Krauts Test Orange ppt. dark red solution in the bottom. Molisch Test

Very light violet solution

In obtaining the results, four tests were used to characterize lipid. The first one is the Liebermann-Burchard test. It is used to detect the presence of steroids. In this test, the acetic anhydride reacts with the third carbon of the hydroxyl group of cholesterol and the steroid is the presence of strong acids that forms the blue-green color of the solution. This test should be done without any single amount of water for it will be contaminated. Another test is the Salkowski test. It is used to identify the presence of the double bond in one cholesterol ring. These rings are the ones that are responsible in the formation of color products in the presence of inorganic acids. The cholesterol is reacted with concentrated sulfuric acid that dehydrates to form the red part in the results. And the last test that was performed was the Molisch test. This is intended to detect the presence of most carbohydrates. It gives a negative result for sugar alcohols(alditols) and 2-deoxy-2amino sugars. The concentrated H2SO4 reacts by hydrolyzing glycosidic bonds present to monosaccharides, which are then dehydrated by the concentrated acid to yield its derivative. The compound produced condense with -naphthol to give a purple complex, which is characteristic of carbohydrates. The exact structure of the condensation products with sulfonated -naphthol (the purple

complex) is unknown. Though during the experiment, the results obtained were of a very light shade of violet. 4. 5. Conclusion References (1) http://people.umass.edu/~mcclemen/581Lipids.html [Retrieved: February 20, 2012] Books: Brown, T., Bursten, B., & LeMay, E. (2004). Chemistry; The Central Science Ninth Edition. New Jersey: Pearson Education (Asia) Pte. Ltd. Brown, T., Bursten, B., Burdge, J., & LeMay, H. (2002). Chemistry; The Central Science Eighth Edition. New Jersey: Pearson Education (Asia) Pte. Ltd. Stoker, H. (2010). Exploring General, Organic, and Biological Chemistry. Singapore 068808: Cengage Learning Asia Pte. Ltd. A.V., Cruz, C.T., et al (2009).Laboratory Manual in Organic Chemistry.Quezon City: C&E Publishing

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