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Position: Post Doctoral Fellow Have you previously received an FAOBMB Travel Fellowship? If yes, please indicate when. No

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Curriculum Vitae Provide a brief CV showing education and research training, including any prizes or awards: (no more than one page) Personal Information: Male, Born - April 24, 1980, Single, SC-Hindu, Indian. Educational Qualification: Post Doctoral Fellowship Banaras Hindu University, Institute of Medical Sciences Varanasi, India Trypanothione reductase from Leishmania Spesies: Purification, Characterization and inhibition by medicinal plant Biomolecules (Protein folding and crystallographic aspects) Banaras Hindu University, Ph.D (Thesis Submitted) Institute of Technology, Varanasi, India Production, Purification and Characterization of a Ribonuclease from Aspergillus niger (Folding and Biological Aspects) M.Sc( Biochemistry) 2003-2005 B.Sc (BioTechnology Biochemistry, Micro Biology), 2000-2003 Intermediate 1998-2000 Personal skills Excellent verbal and written communication skills, keen to learn, good team working ability, flexible in approach, ready to accept challenges, capability to learn new things to explore future possibilities. Technical skills Fermentation, Biochemical characterization of proteins and enzyme kinetics. Protein purification using ion exchange, gel filtration, Gas chromatography Electrophoresis (native, SDS-PAGE, SDS-PAGE) and isoelectric focusing. Protein conformational studies using biophysical tools such as Circular Dichroism, Fluorescence spectroscopy, absorption spectroscopy, differential scanning calorimetry, protein unfolding and renaturation studies and other biophysical techniques. Board of Intermediate Education A.P 65.2% Acharya Nagarjuna University Sri KrishnaDevaraya University. Ananthapur A.P 60.75% 62.5%

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Protein modeling (Protein-protein Docking, simulation, dynamics), Structurefunction relationship study of proteins. Cell culture study (Microscopic Observation (Cytotoxicity), Colony Formation, Cell Migration and Invasion through Matrigel.

Computaional skills MS Office, Biological Databases and Sequence Alignment Homology Modelling. Protein secondary structure prediction etc (still learning). Membership: 1). Indian Biophysical Society (Life Membership No:- 698 from 2009). 2). Scientific and Technical Committee & Editorial Review Board member on Biological and Life Sciences. Awards 1). Selected for Leading Scientists of the World 2012 from England. 2). UGC-DS Kothari Post Doctoral Fellowship. Built Protein Models deposited in PMDB (Protein Model Data Base)

PMDB ID

Protein Name

Organism name

PM0076360

Ribonuclease (A.niger RNase)

Aspergillus niger ATCC26550 Human

PM0076361

Homosapiens Actin

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List of Publications in International Journals* * Number all publications in international journals or book chapters, with earliest first and most recent at the end. Provide the Impact Factor (IF) of the journal and indicate your contribution the work described and the publication. Use as many pages as required. Follow the style in these examples: 1. Extracellular poly (A) specific ribonuclease from Aspergillus niger ATCC 26550: purification, biochemical, and spectroscopic studies.Gundampati R.K, Anurag Sharma, Moni Kumari, Debnath M.*, Process Biochemistry 46 (2011) 135-141. (I.F2.6) 2. Equilibrium unfolding of A. niger RNase: pH dependence of chemical and thermal denaturation. Gundampati R.K, Anurag Sharma, Moni Kumari, M V Jagannadham, Debnath M* European Biophysics Journal. 40 (2011) 923-935.(I.F-2.38) 3. Protein-Protein Docking on Molecular Models of Aspergillus niger RNase and Human Actin: Novel target for Anticancer Therapeutics. Ravi Kumar Gundampati , Chikati Rajasekhar, Moni Kumari, Anurag Sharma, D. Devi Pratyush, M V Jagannadham, Chitta Suresh Kumar*, Debnath M* Journal of Molecular Modeling 18 (2011) 653-662. (I.F-1.79) 4. Optimization of media composition for D-amino acid oxidase production by Trigonopsis variabilis using biostatistical analysis. Neeraj Gupta, Ravi Kumar Gundampati and M Debnath*. accept for publication in Indian Journal of Biochemistry & Biophysics. Vol.49. August 2012. (I.F-1.17) 5. Extracellular ribonuclease from Aspergillus niger: process optimization for production, Gundampati R.K, Debnath M, International Journal of Engineering and Technology. Vol. 1, No.4, October, 2009. (I.F-0) 6. Surface charge based rapid method for detection of microbial contamination in drinking water and food products. Kandpal. M., Gundampati R.K., Debnath M. Wrold Academy of Science Engineering and Technology, Volume 58 October 2009 ISSN 2070-3724. (I.F-0) 7. Extracellular ribonuclease production from Aspergillus niger: process optimization by biostatistical analysis.Gundampati R.K, Swati Singh, Neeraj Gupta, Debnath M, International Journal of Biotechnology and Biochemistry 7 (2011) 279-291.(I.F-0) 8. Protein-Protein Docking on Molecular Models of Aspergillus niger RNase and Human Actin: Novel target for Anticancer Therapeutics. Ravi Kumar Gundampati, Chikati Rajasekhar, Chitta Suresh Kumar*, Debnath M*(Abstract published) in Journal of Biotechnology Volume 150, Supplement 1, November 2010, Page 424. (I.F-3.1)

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9. Screening of Novel inducer for D-amino acid oxidase by Trigonopsis variabilis. Neeraj Gupta, Ravi Kumar Gundampati, Debnath M*. International Journal of Bioscience, Biochemistry and Bioinformatics. Vol. 2, No. 3, May 2012. (I.F-0) 10. Molecular modeling, docking and dynamics simulations of A.niger RNase from Aspergillus niger ATCC26550 for potential prevention of Human Cancer Gundampati Ravi Kumar*, M V Jagannadham, Mira Debnath (Das). (Under review in Journal of Molecular Modeling).(I.F-1.79) 11. Homology model and docking studies on Aspergillus niger RNase and Leishmania donovani Actin: Novel target for Antileishmanial activity. Ravi Kumar Gundampati*, Medicherla V. Jagannadham.(Under review in Journal of Molecular Graphics and Modeling) (I.F-2.18) 12. Molecular docking study on the interaction between Trypanothione reductase and Mangiferin for antileishmanial activity. Ravi Kumar Gundampati, Medicherla V. Jagannadham (Commonicated in Journal of Medical Hypotheses) (I.F-1.19) 13. Antioxidant, cytotoxic activities of Nymphaea pubescens and Isolation of Novel sterol compound from Methanol Extract. Kiran Kumar Angadi, Ravi Kumar Gundampati, Medicherla V. Jagannadham, Ammani Kandru.(Communicated in Journal of Chemistry)(I.F-0.58)

Full Paper presentations(Oral presentation) : 1. International Conference on Bioinformatics, Computational and Systems Biology ICBCSB-2009, Singapore, August-26th -28th, 2009. Extracellular ribonuclease production from Aspergillus niger: process

optimization by biostatistical analysis. 2. Biotech 2011 & 5th Czech-Swiss Symposium with Exhibition (Biotech-2011) Prague, Czech Republic. 15-17 June, 2011. Molecular modeling, docking and dynamics simulations of A.niger RNase from Aspergillus niger ATCC26550 for potential prevention of Human Cancer

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Poster presentation : 1. International Conference on Bioinformatics & Drug Discovery, Instructional Workshop in Bioinformatics & Drug Discovery, (Bio Convene 2007), University of Hyderabad, Hyderabad, on December 19th-22nd, 2007. 2. 3rd International Congress on Bioprocesses in Food Industries & 5th Convention of The Biotech Research Society of India, Osmania University, Hyderabad, India, November 6th-8th 2008. Extracellular ribonuclease from Aspergillus niger: Process optimization for production and activity. 3. International Seminar Plant Biotechnology and Genomics towards a new green Revolution (ISPBG 2009), Yogi Vemana University, Kadapa, Andhra Pradesh, India, on February 6th and 7th 2009. Extracellular ribonuclease production from Aspergillus niger: process

optimization by biostatistical analysis. 4. Food Denmark PhD Congress 2009 November 23rd and 24th, Denmark Surface charge based rapid method for detection of microbial contamination in drinking water and food products. 5. 14th International Biotechnology Symposium and Exhibition-2010(ISB-2010) 14-18 September 2010, Palacongressi, Rimini Italy. Protein-Protein Docking on Molecular Models of Aspergillus niger RNase and Human Actin: Novel target for Anticancer Therapeutics 6. World Congress on Biotechnology March 21st -23rd 2011, Hyderabad, India. Anticancer activity of A.niger RNase (T2-family) from Aspergillus niger ATCC26550 Workshops : 1. International Conference on Bioinformatics & Drug Discovery, Instructional Workshop in Bioinformatics & Drug Discovery, (Bio Convene 2007), University of Hyderabad, Hyderabad, on December 16th-19th, 2007. 2. Workshop and Hands-on Training Course on Molecular Techniques in cell Signaling and Biomedical Nanotechnology Banaras Hindu University, Varanasi, on January 27-31, 2010.

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3.

National Symposium-cum-Workshop on X-Ray Crystallography, Banaras Hindu University, Varanasi, on March 08-09, 2010.

Participated in the Conference : 1. National Seminar on Fertilizer Technology 21st Century Challenges and Options, Banaras Hindu University, Varanasi, on March 7-9th, 2008. 2. Conference on Disorder, Complexity and Biology II (DISCOMB09), Banaras Hindu University, Varanasi, on January 5-8th, 2009. 3. The National Symposium on Cellular and Molecular Biophysics, IBS-2009(NCMB, IBS-2009), Centre for Cellular and Molecular Biology, Hyderabad, January 22nd to 24th, 2009. 4. 7th ITSCON 2010 Annual Meeting of India Thyroid Society, Institute of Medical Sciences, Banaras Hindu University, Varanasi, on February 13-14, 2010. 5. Indian Aging Congress 2010, Banaras Hindu University, Varanasi, on November 12th -14th, 2010. 6. National Seminar on Application of Microbes in Management of Agriculture and Environment (AMMAE), Sri Krishnadevaraya University, Anantapur, on March 4th-6th, 2011.

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Abstract Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract) Titel: Homology model and docking studies on Aspergillus niger RNase and Leishmania donovani Actin: Novel target for Antileishmanial activity Ravi Kumar Gundampati*, Medicherla V. Jagannadham Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi221005, India. ABSTRACT The 3D models of L. donovani actin protein and A.niger RNase were designed using the templates ACTBIND (PDB ID: 3D3Z) and Structure of the yeast actin-human gelsolin segment 1 complex (PDB ID: 1YAG), respectively in Modeller9v8. These models are testified using several validation methods including PROCHECK, ERRAT, WHAT-IF, PROSA2003 and VERIFY-3D. The stereo-chemical quality of the models was judged by Ramachandran plot with PROCHECK. The total quality G-factor 0.3, shows a good quality model. The ERRAT score for the Leishmania donovani actin and A.niger RNase models are 86.104 and 84.615 respectively fit well within the range of a high quality model. The ERRAT score for the templates 1YAG and 3D3Z are 98.039 and 97.391, respectively. The WHAT-IF evaluation justifies a reasonable homology model structure as none of the scores for each residue in the homology model is lower than 5.0. The energy-minimized model of Leishmania donovani actin with PROSA reveals the Z-score value 9.96 between native conformations of the crystal structures. The VERIFY 3D average score is 0.36. All evidences suggest that the geometric quality of the backbone conformation, the residue interaction, the residue contact and the energy profile of the structures were well within the limits of reliable structures. The interaction energy of docking was calculated using the HEX server. The Etotal, lowest docked energy, and calculated RMSD values were -1.902 kcal/mol, 9.323 kcal/mol and 0.402 , respectively. The study presented in the current project may be useful to design molecules that may have antileishmanial activity.

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Personal Statement Indicate briefly here your Research Interests and Career Goals, why you are interested to participate in the YSP Program (including what you will bring to the YSP and what you hope to gain from it): (no more than one page) Research interests My main interests in Structural biology of proteins, Purification and

Characterizations (Biochemical and Biophysical) of protein from plant and microbial. Protein folding study, Structure prediction and protein modelling (Protein-protein and Protein-ligand study (Chemical compounds and plant bioactive molecules) Docking simulation, dynamics), Structure-function relationship study of proteins, Cell culture study, Fermentation. Career Goals My career goal is become a good research in molecular biology and biophysics (Protein folding and refolding studies). Why you are interested to participate in the YSP Program I am very interested to attending for this Young Scientist Program it covers a wide spectrum of topics of current interest in Biological Science (Molecular Biology, Nanotechnology, Biotechnology, Structural biology). Seven days a plenary lecture given by outstanding scientists and Nobel laureates whose exceptionally creative work opens up new frontiers for biotechnology field. In addition, there will be oral and poster sessions allowing for the viewing of a large number of oral and poster presentations. Here I will be exposed to new dimensions of industrial biotechnology which will give me new vision of research in biology field and improve my knowledge. Attachments: Letters of recommendation from two referees.

Submission methods: You can apply in either of two ways: by sending an email with scanned attachments to the Chair of the FAOBMB Fellowship Committee, Prof. Piamsook Pongsawasdi: piamsook.p@chula.ac.th or piamsook.p@gmail.com

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This is the preferred method. If using this method, please assemble the Application Form and the Attachments into a single PDF file. by sending a hard copy of the application form and supporting documents to: Professor Piamsook Pongsawasdi, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7 Hours). Applicants will be notified by email of the decision of the Committee by no later than 15 September, 2012.

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