Curriculum Vitae Provide a brief CV showing education and research training, including any prizes or awards: (no more

than one page) Academic Qualifications

DEGREE M.Sc (Molecular Biology and Biotechnology) B.Sc (Biotechnology) All India Senior School Examination (12th ) All India Secondary School Examination (SSE 10th ) UNIVERSITY/ BOARD Tezpur Central university Andhra University Jawahar Navodaya Vidyalaya (Pedavegi) Jawahar Navodaya Vidyalaya (Pedavegi) % OF MARKS 78.1 74.43 63 61.2 YEAR April, 2008 April, 2005 April, 2002 March, 2000

RESEARCH TRAINING M.Sc. dissertation: Statistical Optimization of Bacillus alcalophilus -amylase Immobilization on Iron-oxide Magnetic Nanoparticles. This work has been published in Biotechnology and Bioprocess Engineering 2010. Project supervisor: Prof. A. K. Mukherjee, Department of Molecular Biology and Botechnology, Tezpur Central University, Assam, India. Ph.D Thesis work: My study includes Regulation of DNA repair in cancer cells. Recently we have shown that BCL2 protein interacts with Ku proteins and decreases nonhomologous DNA end joining (NHEJ) in cancer cells. This work has been published in Journal of Biological Chemistry, 2010. I am also interested in characterizing DNA double-strand break repair in mammalian mitochondria. Interestingly we found that microhomology mediated DNA end joining was the predominant pathway for repair of DSBs in mitochondria. I am also interested in the identification of novel NHEJ proteins through protein fractionation studies from mammalian testicular extracts. Principal investigator: Dr. Sathees C. Raghavan, Department of Biochemistry, Indian Institute of Science, Bangalore, India. CONFERENCES ATTENDED: Presented a poster on Anti-apoptotic Protein, BCL2, downregulates nonhomologous DNA end joining in cancer cells at the Society of Biological Chemists (India) conference held at Indian Institute of Science, Bangalore on Dec 13-15, 2010. Given oral presentation on Anti-apoptotic Protein, BCL2, downregulates nonhomologous DNA end joining in cancer cells at Life Science Symposium held at Bhabha Atomic Research Centre, Mumbai on Dec 22-24 2010.

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List of Publications in International Journals* * Number all publications in international journals or book chapters, with earliest first and most recent at the end. Provide the Impact Factor (IF) of the journal and indicate your contribution the work described and the publication. Use as many pages as required. Follow the style in these examples:

1. Ashis K. Mukherjee, Tadi Satish Kumar, Sudhir K. Rai and Jetendra K. Roy. Statistical optimization of Bacillus alcalophilus -amylase immobilization on iron oxide magnetic nanoparticles. Biotechnology and Bioprocess Engineering, 15, 984-992 (2010) (IF 1.004) TSKs contribution to this work was, Isolation and partial purification of -amylase from a thermophilic B. alcalophilus Assay of -amylase activity Covalent coupling of -amylase onto iron-oxide magnetic nanoparticles Characterization of the free and -amylase immobilized MNPs Kinetic properties, thermal and storage stabilities, and reusability

2. Tadi Satish Kumar, Vijayalakshmi Kari, Bibha Choudhary, Mridula Nambiar, Akila T. S. and Sathees C. Raghavan. Anti-apoptotic Protein, BCL2, downregulates nonhomologous DNA end joining in cancer cells, J. Biol. Chem., 285: 32657-32670 (2010). (IF 5.498). 3. Tadi Satish Kumar and Sathees C. Raghavan. Microhomology-mediated nonhomologous repair of DNA double-strand breaks in mammalian mitochondria. (manuscript under consideration). 4. Tadi Satish Kumar and Sathees C. Raghavan. Partial purification and characterization of nonhomologous DNA end joining (NHEJ) proteins shows that NHEJ in rat kidney is regulated by novel factors. (manuscript under consideration).

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Abstract
Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract)

Microhomology-mediated nonhomologous repair of DNA double-strand breaks in mammalian mitochondria Tadi Satish Kumar and Sathees C. Raghavan Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India E-mail: sathees@biochem.iisc.ernet.in, satish_dlp@biochem.iisc.ernet.in

Abstract Mitochondrial DNA deletions are associated with various mitochondrial disorders. Mitochondrial DNA (mtDNA) deletions identified in humans are flanked by short directly-repeated sequences, however; the mechanism by which these deletions generated are unknown. Compared to nuclear DNA (nDNA), mtDNA is highly exposed to oxidative damage due to its proximity to the respiratory chain and the lack of protective histones. Double strand breaks (DSBs) generated by reactive oxygen species, replication stalling or radiation represents a highly dangerous form of damage to both nDNA and mtDNA. Although, DSB repair in nuclear DNA is well studied, little is known about DSB repair in mitochondria. In the present study, we investigated DSB repair mechanisms in mitochondria using cell-free extracts prepared from purified mitochondria. Results showed that while clssical NHEJ was undetectable altertnative NHEJ (A-NHEJ) was efficient in correction of DSBs in mitochondria. A-NNEJ in mitochondria was dependent on microhomology. Immunoprecipitation (IP) and inhibitor assays suggested that CtIP, FEN1, MRE11, PARP1 and DNA LIGASE III proteins are involved during A-NHEJ in mitochondria. More importantly, knockdown experiments showed the involvement of DNA Page 4 of 7

LIGASE III in mitochondrial A-NHEJ. Further, colocalization studies showed ANHEJ proteins localize in to mitochondria within cells. These observations highlight the central role of A-NHEJ in maintenance of the mammalian mitochondrial genome and this knowledge will be crucial for the development of future therapeutic strategies to combat a variety of human diseases associated with mitochondria.

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Personal Statement
Indicate briefly here your Research Interests and Career Goals, why you are interested to participate in the YSP Program (including what you will bring to the YSP and what you hope to gain from it): (no more than one page)

Research Interests James D. Watson and Francis Crick, discovered the DNA structure more than 50 years ago. The mechanisms that preserve the genetic information encoded by DNA and its faithful transmission across generations have been an active area of investigation since then. Maintenance of genomic integrity is essential for the survival of the cell and it must be protected from various types of DNA damages induced by physiological or pathological agents. It has been estimated that a cell can undergo up to 105 spontaneous DNA lesions per day. My research interests include understanding the most lethal types of DNA damage and its repair i.e. DNA double-strand break (DSB) repair, because unrepaired DSBs are particularly lethal to the cell as they can lead to genome rearrangements leading to cancer or cell death. I am also interested in understanding DSB repair in mitochondria of normal tissues, as we know mitochondrial DNA (mtDNA) deletions are associated with various types of mitochondrial disorders related to cancer, aging, diabetes, deafness, neurodegenerative disorders, sporadic and inherited diseases. Approximately 85% of these deletions in humans are directly linked to mitochondrial disorders but the mechanisms by which these deletions arise are still an active area of interest. Another major objective of the current study is to fractionate and identify novel nonhomologous DNA end joining (NHEJ) factors involved in catalyzing efficient NHEJ which will help us in better understanding and regulation of NHEJ in higher eukaryotes. Career Goals Upon completion of my Ph.D., I aspire to obtain a postdoctoral position at a research university/institute whereby I would continue to develop the skills required to be a fully independent scientist in the area of genomic instability and DNA repair. I will seek opportunities and fellowships that will allow me to gain more teaching experience at the undergraduate and graduate levels. I am committed to a productive career in the DNA repair research field. The benefit expected to be derived by attending this event YSP Programme is focused on different topics in biochemistry and molecular biology. Attending this programme would increase my awareness of current and emerging challenges in science. I am pursuing Ph.D on regulation of nonhomologous DNA end joining (NHEJ) in different cancer cells, understanding double-strand break repair in mitochondria of normal tissues and in the identification of novel proteins involved in NHEJ pathway. This conference will give me a platform to present my work and get valuable inputs on my work. It will also give me a wonderful opportunity to listen to great talks by leading scientists of the world. This conference will give me an exposure, an opportunity to interact and share views with international scientific community.

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Attachments: Letters of recommendation from two referees.

Submission methods: You can apply in either of two ways: by sending an email with scanned attachments to the Chair of the FAOBMB Fellowship Committee, Prof. Piamsook Pongsawasdi: piamsook.p@chula.ac.th or piamsook.p@gmail.com This is the preferred method. If using this method, please assemble the Application Form and the Attachments into a single PDF file. by sending a hard copy of the application form and supporting documents to: Professor Piamsook Pongsawasdi, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7 Hours). Applicants will be notified by email of the decision of the Committee by no later than 15 September, 2012.

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DEPARTMENT OF BIOCHEMISTRY INDIAN INSTITUTE OF SCIENCE BANGALORE 560 012, KARNATAKA, INDIA Desirazu N Rao Professor Chairman, Division of Biological Sciences Tel: +91-80-2293 2538 Fax: 91-80-2360 0416 dnrao@biochem.iisc.ernet.in

It is with immense pleasure that I recommend Tadi Satish Kumar for the YSP-Travel Fellowship for the 13th FAOBMB Congress in Bangkok to be held during 23-29 November this year. Satish joined the department of biochemistry here August 2008 after having completed his Masters in Biotechnology from Tezpur Central University. He has done extremely well in his academics. Satish has been working in my colleague Dr. Sathees Raghavans laboratory. His Ph.D research work mainly focuses on Nonhomologous repair of DNA double-strand breaks in mammalian mitochondria. So far his research work highlights the central role of Nonhomologous end joining in maintenance of the mammalian mitochondrial genome. He has published some of this work in the Journal of Biological chemistry and I understand atleast two more publications will come out of his research work. His commitment and perseverance to research impressed me the most. Satish's capacity to think with a clear and focused mind is an attribute I have witnessed. He is well prepared for any task and his fundamentals are firmly in place. He also has a keen questioning mindset that is essential for independent research. His behavior with fellow members of the lab is very good and he gets along with everyone and always provides a helping hand when needed. I strongly recommend him for the YSP Travel fellowship. I believe his participation in the FAOBMB congress would give him an opportunity to interact with scientists from different parts of the AsiaPacific region and more importantly give Satish an exposure to international science and scientists.

Sincerely yours,

(Desirazu N Rao) July 9, 2012