APPLICATION FORM

YSP-Travel Fellowships for 13th FAOBMB Congress, Bangkok, 23-29 November, 2012.
Name of Applicant: Dr. Chanthakan Nuchsuk Position: Postdoctoral Fellow : training as a research assistant at Department of Biochemistry, Faculty of Science, Kasetsart University)

Address of Applicant: (Normally this will be your institutional or office address) Department of Biochemistry, Faculty of Science, Kasetsart University 50 Ngam-Wong-Wan Rd., Ladyaow, Chatuchak, Bangkok 10900 Email address: g5084028@ku.ac.th, pui.chanthakan@gmail.com Membership of FAOBMB Constituent Society: Biology Biochemistry and Molecular Biology Section, The Science Society of Thailand Under the Patronage of His Majesty the King Date of Birth: June 11, 1982

Proof of Age: (Certification by the Head of your Institution or Department is required) I certify on the basis of official documentation which I have seen (e.g. passport, birth certificate or drivers licence) that the date of birth of the applicant Dr. Chanthakan Nuchsuk is 11 June 1982, as shown above.

Signed: (Associate Professor Sunanta Ratanapo) Position: Associate Dean for Research, Faculty of Science, Kasetsart University

Have you previously received an FAOBMB Travel Fellowship? If yes, please indicate when. I have previously received an FAOBMB Travel Fellowship at 12th FAOBMB in 2011.

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CIRRICULUM VITAE
Personal Information: Name Date of Birth Place of Birth Marital Status Citizenship Religion : Miss Chanthakan Nuchsuk : June 11, 1982 : Nakhonsawan, Thailand : Single : Thai : Buddhist

Contact Information: Department of Biochemistry Tel. +66-2562-5555 ext.2033 Faculty of Science Fax. +66-2561-4627 Kasetsart University Email: pui.chanthakan@gmail.com 50 Ngam Wong Wan Rd., Ladyaow, Chatuchak , Bangkok, Thailand 10900 Educations: 2001-2005: B.Sc. (Biochemistry and Biochemical Technology) Special Problem Title: Methyl Ester Production from Some Vegetable Oils Catalysed by Boron Trifluoride Advisor: Ms. Woraluk Angsuwarangoon Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand M.Sc. (Biochemistry) Thesis Title: Feed Development Using Digestive Enzyme Technology for Culture of Shark Catfish, Helicophagus leptorhynchus Ng&Kottelat, 2000 Advisor: Assist. Prof. Dr. Arunee Engkagul Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand Ph.D. (Biochemistry) Thesis Title: Purification and Characterization of RibosomeInactivating Protein from Seed Coat of Jatropha curcas Advisor: Assoc. Prof. Dr. Sunanta Ratanapo Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand

2005-2007:

2007-2012:

Scholarships/Awards: 1. 2. 3. Dr. Tab Neelanithi Foundation Award for qualified M.Sc. student (First Place Winner Award for Excellence in Biochemistry) (2007) Graduate Thesis Research Scholarships from the Graduate School, Kasetsart University (2009) FAOBMB Travel Fellowships from Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) (2011)

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FAOBMB Fellowships from Biochemistry and Molecular Biology section, The Science Society of Thailand under the Patronage of His Majesty the King (2011)

Training/Workshops: Workshop on Protein Bioinformatics, May 16-19, 2006. Department of Biochemistry, Faculty of Science, Kasetsart University, Thailand Workshop on Glycomics: Biochemistry and Analysis of Glycans, September 8-12, 2008. Department of Biochemistry, Faculty of Science, Kasetsart University, Thailand

Conferences: 1. Chanthakan Nuchsuk, Arunee Engkagul, Uthaiwan Kovitvadhi and Orapin Jinthasathaporn. 2006. Characterization of digestive enzymes in juvenile shark catfish Helicophagus leptorhynchus Ng&Kottelat, 2000. In the 1st Symposium of Fisheries Fisheries and Aquatic Resources for Security and Stability, December 22-23, 2006, Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Chiang Mai, Thailand. Chanthakan Nuchsuk, Thananya Thitiprasert, Sittiruk Roytrakul, Kiattawee Choowongkomol and Sunanta Ratanapo. 2008. Purification of a new hemeagglutinin protein from seed coat of physic nut. In Third Annual Symposium of Protein Society of Thailand Frontiers in Protein Research, August 28-29, 2008, Chulabhorn Research Institute Conference Center, Bangkok, Thailand. Chanthakan Nuchsuk, Thananya Thitiprasert, Nuanchawee Wetprasit, Sittiruk Roytrakul and Sunanta Ratanapo. 2008. Antibacterial activities of a hemeagglutinin protein from physic nut. In The 34th Congress on Science and Technology of Thailand, October 30 - November 2, 2008, QueenSirikit National Convention Center, Bangkok, Thailand. Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul and Sunanta Ratanapo. 2009. New method to determine the enzymatic activity of ribosome-inactivating proteins. In the 2nd Biochemistry and Molecular Biology Conference for Regional Sustainable Development, May 7-8, Khon Kaen University, Khon Kaen, Thailand. Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul, Chotika Yokthongwattana and Sunanta Ratanapo. 2011. Larvicidal activity effect of seed coat protein from Jatropha curcas to the mosquito, Aedes aegypti. In the 3rd Biochemistry and Molecular Biology (BMB) international conference From basic to translational research for a better life, April 6-8, 2011, The Empress convention centre, Chiang Mai, Thailand. Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul, Chotika Yokthongwattana and Sunanta Ratanapo. 2011. Toxicity comparison of the aqueous extracts from seeds of Ricinus communis and Jatropha curcas against the mosquito, Culex quinquefasciatus. In the 22nd Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) international conference, October 5-7, 2011, The Matrix Building, Biopolis, Singapore.

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List of Publications: 1. Chanthakan Nuchsuk, Arunee Engkagul, Uthaiwan Kovitvadhi and Orapin Jinthasathaporn. 2006. Characterization of digestive enzymes in shark catfish Helicophagus leptorhynchus Ng&Kottelat, 2000. In Proceeding of 45th Kasetsart University Annual Conference, Kasetsart University, Thailand. Chanthakan Nuchsuk, Arunee Engkagul, Uthaiwan Kovitvadhi and Orapin Jinthasathaporn. Characterization of digestive enzymes in juvenile shark catfish Helicophagus leptorhynchus Ng&Kottelat, 2000. Journal of Fisheries Technology Research. 1(2), 261-272 (2007) Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul and Sunanta Ratanapo. Larvicidal activity of a toxin from the seeds of Jatropha curcas Linn. against Aedes aegypti Linn. and Culex quinquefasciatus Say. Tropical Biomedicine 29(2), 286-296 (2012) Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul and Sunanta Ratanapo. Insecticidal activity of crude protein extract and purified protein from seed coat of Jatropha curcas against two lepidoptera species, Sodoptera litura (Fabricius) and Spodoptera exigua (Hbner). (Manuscript under preparation) Chanthakan Nuchsuk, Nuanchawee Wetprasit, Sittiruk Roytrakul, Nattanan Panjavorayan, Kiattawee Choowongkomon, Chotika Yokthongwattana and Sunanta Ratanapo. 2012. Bioactivities of Jc-SCRIP, a novel toxic ribosomeinactivating protein from Jatropha curcas seed coat. (Manuscript under preparation)

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Petty patent:

( 1103001131)

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Abstract

BIOACTIVITIES OF THAI RICE PROTEIN HYDROLYSATES

Nuchsuk C1, Wetprasit N2, Roytrakul S3, Tuntithadapitak C1 and Ratanapo S1*
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Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand. Department of Biotechnology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand. 3 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand. First author: pui.chanthakan@gmail.com, *Corresponding author: fscisnr@ku.ac.th

Several plant proteins are sources of bioactive peptides that often present antioxidant activities. These peptides are encrypted in an inactive form within the sequences of such proteins from which they are released during either in vivo or in vitro digestion. Rice, a main staple food for the world population consists of 7-9% protein by weights. The main sources of the rice proteins are endosperm and bran (a co-product from the milling of rice). This study aims to explore for some bioactive peptides with antioxidant and anticancer activities against gastric cancer from some local Thai rice. Patterns of the rice total proteins were preliminary examined from the endosperm and bran of 16 Thai rice varieties by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Two major endosperm protein bands of 16.62 and 24.92 kDa were found in 9 varieties of the rice and more bands of 57.94 and 21.50 kDa were found in the another 7 varieties. Four major bran protein bands of 18.35, 28.98, 44.66 and 63.21 Kda were found in almost varieties of the rice, except PL2, KD29 and KKD which lost the band of 63.21 Kda. The different between protein patterns of rice endosperm and bran revealed the different of the peptide fragments obtained by hydrolysis of the proteins with a digestive enzyme. Degree of the enzyme hydrolysis, antioxidant activities and cytotoxicities of the peptides was performed and the biological active of some peptides will be further identified. The knowledge from this study will be advantage to value-added of the rice for food supplements or disease preventive propose. Keywords: Thai rice, protein hydrolysate, bioactivities Acknowledgement: This work is a part of Rice subproject: Preparation of bioactive peptides from rice endosperm and rice bran, financially supported by the Agricultural Research Development Agency (ARDA). References: 1. Agboola, S., Ng, D., Mills, D. (2005) Characterisation and functional properties of Australian rice protein isolates. J. Cereal Sci. 41, 283-290. 2. Adebiyi, P.A., Adebiyi, A.O., Hasegawa, Y., Ogawa, T., Muramoto, K. (2009) Isolation and characterization of protein fraction from deoiled rice bran. Eur. Food Res. Technol. 228, 391-401. 3. Zhang, J., Zhang, H., Wang, L., Guo, X., Wang, X., Yao, H. (2010) Isolation and identification of antioxidative peptides from rice endosperm protein enzymatic hydrolysate by consecutive chromatography and MALDI-TOF/TOF MS/MS. Food Chem. 119, 226-234. Page 5 of 7

Personal Statement My present research interest is screening and characterization of some bioactive peptides derived from rice (Oryza sativa) endosperm and rice bran (a co-product from the milling of rice) of various Thai rice varieties. It is proposed that different rice varieties have different proteins and the different rice proteins may release different peptides having different in their biological properties. The highly potency antioxidative, antibacterial and anticancer peptides will be separated and identified for further applications in food supplement or disease preventive propose. Many natural proteins possess the functional properties by bioactive peptides encrypted in the protein molecules, which can be released during the gastrointestinal digestion by digestive enzymes or by proteolytic processes in controlled hydrolysis using exogenous proteases. So far, many protein-derived peptides of plant origin with relevant antihypertensive, antiviral, antibacterial and anticancer potential have been reviewed. Rice proteins are about 7-9% by weights and they are rich in lysine. The main sources of proteins in rice are endosperm and bran. The rice endosperm is one of the most important foods of the world population and the rice bran is commonly used as animal feed. Most studies of rice proteins have been targeted towards the biosynthesis and deposition of them, however, only few were reported about the rice peptides. In my present study, mixed peptides from rice endosperm and rice bran of various Thai rice varieties have been obtained by proteolysis with a digestive enzyme, pepsin. The degree of hydrolysis and bioactivities including antioxidative activities, antibacterial activities against some human-pathogenic bacteria and cytotoxicities to a normal cell line and some cancerous cell lines have being determined. My career goal is to become a full-time postdoctoral researcher (academic/industrial) for one to three years, where I may gain my knowledge, training of new technology and increase my experimental skills. I dream to have a chance to work in an exotic location aboard which I may increase my understanding of biochemistry and what I discover may be put to practical use to find solutions of specific problems. After that, I wish to become a lecturer teaching basic and advanced biochemistry courses in a university or college, and also mentor students with projects and direct research programs. I certainly believe I will receive more research experience, critical thinking and international net work with the other biochemists from the YSP programme. My experience will be transferred to the Thai students and benefit to my future biochemical research development in Thailand.

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To whom it may concern,

Sittiruk Roytrakul, Ph.D. National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd., Klong 1, Klong Luang, Pathumthani

1 August, 2012

I am privileged to write a formal recommendation in support of Miss Chanthakan Nuchsuk for YSP-Travel Fellowships for 13th FAOBMB Congress. As a member of the Ph.D. committee, I have known her for approximately four years. I consider her to be one of the most studious and responsible students. I witnessed her tremendous growth and development not only in the area of science achievement and leadership, but also in maturity and character. As for these reasons, I recommend Miss Chanthakan Nuchsuk to the YSP-Travel Fellowships with absolute confidence and hope that you will carefully consider admission application.

Best Regards,

Sittiruk Roytrakul