Specifications for

FS-Series
Fermentors

PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION

3, Hagavish st. Israel 58817 Tel: 972 3 5595252, Fax: 972 3 5594529 mrc@mrclab.com

MRC.VER.01-4.11

Bench-top fermentor selection guide

Jacket vessel (Dish bottom) Single wall with blanket
Single wall (Plain bottom)

Single wall (Dish bottom)

Air lifter

Mammalian Cell Culture Aerobic Microorganism Culture (Note 1) Micro-aerobic Microorganism Culture (Note 2) Anaerobic Microorganism Culture (Note 3) To culture Fragile Cell (Note 4) Photosynthesis Culture (Note 5) Plant Cell Culture Insect Cell Culture

V V V V O X V V
O : Well

V V V V O X V V

X V V V X O X X

X V V V X V V O

X O X X V V V X

V : Excellent

X : Not Recommended

Note: 1. Some Bacteria; Yeast; Fungi 2. Some Lactobacillus; for ethanol production 3.Same as note 2 4. This vessel is excellent for some cell are so fragile and easy shear by any type of mechanical impeller 5. Plant; Algae; Cyanobacteria (blue-green algae)

Field Medicine Products Agriculture Products Food Additive Products Chemical And Energy Products For Environment Products Other Product Biomass Products Enzyme

Products Antibiotics; Hormones; Vaccine; Immune Modulators; Blood Proteins etc., Veterinary Medicine; Biotec Fertilizer; Microbial Insecticides etc., Amino Acids ; Vitamins ; Organic Acids etc., Ethanol; Glycerol; Methane etc., Digest Waste Microorganism Bioleaching Of Metals; Genetically Engineered Spiders; Silk Proteins Bakers Yeast; Mushrooms; Algae; Single-Cell Proteins etc., Amylases; Celluloses; Proteases; Microbial Rennin; Lipases; Glucose Isomerase; Penicillin Acylase; Cholesterol Oxidase etc., Fermentation Products Ethanol; Lacto Acid; Butanol; Acetone Growth Factors Amino Acid; Vitamins; Citric Acid etc., Secondary Metabolites Antibiotics; Alkaloids etc., Gene ProductsInsulin; Human Growth Hormone; Interferons; Interleukins; Blood-Clotting Factors; Serum Albumin; Hepatitis B Vaccine; Rabies Vaccine And Diarrhea Vaccine For Pigs; Monoclonal Antibodies etc.,
48

Metabolism Products

Dryheatingsystem(Plainbottomtypevessel)

Singlewallvessel (Plainbottom) Vessel

Totalvolume(Liter) Material Controlpanel StorageProgram Controlunit Datastorage Cabinetmaterial Dimension Ratedvoltage InletGasFlowmeter Aeration Impeller Sparger Baffle Heating Cooling Temperature Range Probe Controlmode Drive Agitation SpeedRange Controlmode

3L 4

5L 7

7L 9.3

10L 13.4

Borosilicateglass/316Lssforheadplateandallfittings 10.4ColorTouchscreenInterface Upto59,994programfordifferentkindsofcondition Upto10datafiles ABSfrontpanelandpaintedironhousing Footprint:400x500mm(WxD);Height:740mm 110Vor220V;50/60Hz 110LPM Orificering Removable316Lstainlesssteelbaffles Bottomheatingplate(Baseunit) Coolingcoil(Internalheatexchanger) 5abovecoolantupto90 PlatinumRTDprobe(Pt100);0~90 Programmable15stepsPIDcontroller RemovableTopbrushlessmotor Twotypeofagitationmotor: a. 301200rpmisforgeneralfermenter; b. 5300rpmisforshearsensitivecellline(optional) Programmable15stepsPIDcontroller

Communicationport RemotecontrolthroughEthernet(SCADA)

Datastorageinterface USBport

220LPM

ThreeadjustableRushtontypeimpellers

Range pH Probe Controlmode Range Probe DO Controlmode

212pH Gelfilledelectrode;Autoclavable Onepointcontrol 0200% PolarographicDOsensor;Autoclavable PID;Cascadefunctiontoresponseto a. Steppingincreasingordecreasingagitationspeed b. OxygenEnrichmentDevice(optional) c. Tostartsubstratefeedingprogram. /+2000mV Gelfilledelectrode;Autoclavable(optional) 316LstainlesssteelwithinsulatedTeflontube; On/Offcontroller Precisesteppingmotor;minimumspeedis1rpm

ORP Foam

Transmitter Probe Probe

Builtin4setsofpumps;Oneexternalpump(optional) Peristaltic pump Speedrange Controlmode Exhaust

OptionalItem:
1. 2. 3. LowSpeedAgitationDriver:5~300rpm OxygenEnrichmentDevice Oneexternalpump Specification: Model#:MUD01 Rpmandincrement:20~300rpm; 1rpm MotorType:Brushlessmotor Numberofroller:1 4. 5. FluorescentLampcontrolIncluded4setsofT5FluorescentLampandaexternalTimeron/offcontrol(24hr) ORPprobe:Gelfilledelectrode;Autoclavable

065rpm Programmable15stepsfeedingcontrol; PumpcanbeassignedforAcid,Base,Antifoamand Substrate 316LStainlesssteelcondenser

UtilityRequirement
1. Buildingmainsupply110V60Hzor220V50Hzwithelectricalsafetycutoffswitch,

 2. 3. 4. Autoclaveforsterilization CoolingCirculatingWaterBathforcoolingpurpose CompressorAirtosupplyair,whichcomeswithareducertocontrolpressuretoaround1kg/cm2.Thisairistobe dry,oilfreeandfiltered. 5. Computerforremovecontrol

Dryheatingsystem(Dishbottomtypevessel)

Vessel

Singlewallvessel (Dishbottom) withblanket

Totalvolume(Liter) Material Controlpanel StorageProgram Controlunit Datastorage Cabinetmaterial Dimension Ratedvoltage InletGasFlowmeter

3L 4

5L 7

7L 9.3

10L 13.4

15L 16.5

20L 23

Borosilicateglass/316Lssforheadplateandallfittings 10.4ColorTouchscreenInterface Upto59,994programfordifferentkindsofcondition Upto10datafiles ABSfrontpanelandpaintedironhousing Footprint:400x500mm(WxD);Height:740mm 110Vor220V;50/60Hz 110LPM 220LPM 550LPM Twodifferenttypeofimpeller a. 3pcsofadjustableRushtontypeimpeller b. 2pcsofadjustablePitchedbladeimpeller
note:

Communicationport RemotecontrolthroughEthernet(SCADA)

Datastorageinterface USBport

Aeration

Impeller

1.

Rushtontypeimpellerisforcelllinethatarenotconsidered shearsensitive

2.

Pitchedbladeimpellerforshearsensitivecellline

3. Thestandardsystemincludesonetypeimpeller.Itisoptionfor anothertype.Itisavailableforcustomermadeimpeller). Sparger Baffle Temperature Heating Cooling Orificering Removable316Lstainlesssteelbaffles Heatingblanket Coolingcoil(Internalheatexchanger);Thefingerheat

exchangerisfor3Lvessel. Range Probe Controlmode Drive SpeedRange 5abovecoolantupto60 PlatinumRTDprobe(Pt100); PIDcontrollerforonepointorupto15steps programmablecontrol. RemovableTopbrushlessmotor Twotypeofagitationmotor: a. 301200rpmisforgeneralfermenter; b. 5300rpmisforshearsensitivecellline(optional) PIDcontrollerforonepointorupto15steps programmablecontrol. 212pH Gelfilledelectrode;Autoclavable Onepointcontrol 0200% PolarographicDOsensor;Autoclavable PID;Cascadefunctiontoresponseto a. Steppingincreasingordecreasingagitationspeed b. OxygenEnrichmentDevice(optional) c. Tostartsubstratefeedingprogram. /+2000mV Gelfilledelectrode;Autoclavable(optional) 316LstainlesssteelwithinsulatedTeflontube; On/Offcontroller Precisesteppingmotor;minimumspeedis1rpm Peristaltic pump Speedrange Controlmode Exhaust
OptionalItem:
1. 2. LowSpeedAgitationDriver:5~300rpm OxygenEnrichmentDevice

Agitation

Controlmode Range pH Probe Controlmode Range Probe DO Controlmode

ORP Foam

Transmitter Probe Probe

Builtin4setsofpumps;Oneexternalpump(optional) 065rpm a. Programmable15stepsfeedingcontrol; b. PumpcanbeassignedforAcid,Base,Antifoamand Substrate 316LStainlesssteelcondenser

 3. Oneexternalpump Specification: Model#:MUD01 Rpmandincrement:20~300rpm; 1rpm MotorType:Brushlessmotor Numberofroller:1 4. ORPprobe:Gelfilledelectrode;Autoclavable

UtilityRequirement
1. 2. 3. 4. Buildingmainsupply110V60Hzor220V50Hzwithelectricalsafetycutoffswitch, Autoclaveforsterilization CoolingCirculatingWaterBathforcoolingpurpose CompressorAirtosupplyair,whichcomeswithareducertocontrolpressuretoaround1kg/cm2.Thisairistobe dry,oilfreeandfiltered. 5. Computerforremovecontrol

Thermostatsystem(Jacketvesseltype)

Jacketvessel (Dishbottom) Vessel

Totalvolume(Liter) Material Controlpanel StorageProgram Controlunit Datastorage Cabinetmaterial Dimension Ratedvoltage InletGasFlowmeter

3L 4

5L 7

7L 9.3

10L 13.4

Borosilicateglass/316Lssforheadplateandallfittings 10.4ColorTouchscreenInterface Upto59,994programfordifferentkindsofcondition Upto10datafiles ABSfrontpanelandpaintedironhousing Footprint:400x500mm(WxD);Height:740mm 110Vor220V;50/60Hz 110LPM 220LPM Twodifferenttypeofimpeller a. 3pcsofadjustableRushtontypeimpeller b. 2pcsofadjustablePitchedbladeimpeller
note:

Communicationport RemotecontrolthroughEthernet(SCADA)

Datastorageinterface USBport

Aeration

Impeller

1.

Rushtontypeimpellerisforcelllinethatarenotconsidered shearsensitive

2. 3.

Pitchedbladeimpellerforshearsensitivecellline Thestandardsystemincludesonetypeimpeller.Itisoptionfor anothertype.Itisavailableforcustomermadeimpeller).

Sparger Baffle Temperature Controlsystem

Orificering Removable316Lstainlesssteelbaffles Thermostatsystem; Builtinheatexchanger(400Wheater/watercirculation

pump);Automaticcoolingwatervalve Range Probe Controlmode Drive Agitation SpeedRange Controlmode Range pH Probe Controlmode Range Probe DO Controlmode 5abovecoolantupto60 PlatinumRTDprobe(Pt100) Programmable15stepsPIDcontroller RemovableTopbrushlessmotor Twotypeofagitationmotor: a. 301200rpmisforgeneralfermenter; b. 5300rpmisforshearsensitivecellline(optional) Programmable15stepsPIDcontroller 212pH Gelfilledelectrode;Autoclavable Onepointcontrol 0200% PolarographicDOsensor;Autoclavable PID;Cascadefunctiontoresponseto a. Steppingincreasingordecreasingagitationspeed b. OxygenEnrichmentDevice(optionalitem) c. Tostartsubstratefeedingprogram. /+2000mV Gelfilledelectrode;Autoclavable(optional) 316LstainlesssteelprotectorwithinsulatedTeflontube; On/Offcontroller PreciseSteppingmotor;min.speedis1rpm Peristaltic pump Speedrange Controlmode Exhaust
OptionalItem:
1. 2. 3. LowSpeedAgitationDriver:5~300rpm OxygenEnrichmentDevice Oneexternalpump

ORP Foam

Transmitter Probe Probe

Builtin4setsofpumps;Oneexternalpump(optional) 065rpm Programmable15stepsfeedingcontrol; PumpcanbeassignedforAcid,Base,Antifoamand Substrate 316LStainlesssteelcondenser

 Specification: Model#:MUD01 Rpmandincrement:20~300rpm; 1rpm MotorType:Brushlessmotor Numberofroller:1 4. ORPprobe:Gelfilledelectrode;Autoclavable

UtilityRequirement
1. 2. 3. 4. Buildingmainsupply110V60Hzor220V50Hzwithelectricalsafetycutoffswitch, Autoclaveforsterilization CoolingCirculatingWaterBathforcoolingpurpose CompressorAirtosupplyair,whichcomeswithareducertocontrolpressuretoaround1kg/cm2.Thisairistobe dry,oilfreeandfiltered. 5. Computerforremovecontrol

Thermostatsystem(Dishbottomtypevessel)

Singlewallvessel (Dishbottom) Vessel

Totalvolume(Liter) Material Controlpanel StorageProgram Controlunit Datastorage Cabinetmaterial Dimension Ratedvoltage InletGasFlowmeter

3L 4

5L 7

7L 9.3

10L 13.4

Borosilicateglass/316Lssforheadplateandallfittings 10.4ColorTouchscreenInterface Upto59,994programfordifferentkindsofcondition Upto10datafiles ABSfrontpanelandpaintedironhousing Footprint:400x500mm(WxD);Height:740mm 110Vor220V;50/60Hz 110LPM 220LPM Twodifferenttypeofimpeller a. 3pcsofadjustableRushtontypeimpeller b. 2pcsofadjustablePitchedbladeimpeller
note:

Communicationport RemotecontrolthroughEthernet(SCADA)

Datastorageinterface USBport

Aeration

Impeller

1.

Rushtontypeimpellerisforcelllinethatarenotconsidered shearsensitive

2. 3.

Pitchedbladeimpellerforshearsensitivecellline Thestandardsystemincludesonetypeimpeller.Itisoptionfor anothertype.Itisavailableforcustomermadeimpeller).

Sparger Baffle

Orificering Removable316Lstainlesssteelbaffles

Controlsystem Temperature Range Probe Controlmode Drive Agitation SpeedRange Controlmode Range pH Probe Controlmode Range Probe DO Controlmode

Thermostatsystem; Builtinheatexchanger(400Wheater/watercirculation pump);Automaticcoolingwatervalve 5abovecoolantupto60 PlatinumRTDprobe(Pt100) Programmable15stepsPIDcontroller RemovableTopbrushlessmotor Twotypeofagitationmotor: a. 301200rpmisforgeneralfermenter; b. 5300rpmisforshearsensitivecellline(optional) Programmable15stepsPIDcontroller 212pH Gelfilledelectrode;Autoclavable Onepointcontrol 0200% PolarographicDOsensor;Autoclavable PID;Cascadefunctiontoresponseto a. Steppingincreasingordecreasingagitationspeed b. OxygenEnrichmentDevice(optionalitem) c. Tostartsubstratefeedingprogram. /+2000mV Gelfilledelectrode;Autoclavable(optional) 316LstainlesssteeldetectorwithinsulatedTeflontube; On/Offcontroller

ORP

Transmitter Probe Probe

Foam

FourbuiltinEasytubeloadingpumps;Oneexternalpump(Optional) PreciseSteppingmotor;min.speedis1rpm Peristaltic pump Speedrange Controlmode Exhaust

OptionalItem:
1. 2. LowSpeedAgitationDriver:5~300rpm OxygenEnrichmentDevice

065rpm Programmable15stepsfeedingcontrol; PumpcanbeassignedforAcid,Base,Antifoamand Substrate 316LStainlesssteelcondenser

 3. Oneexternalpump Specification: Model#:MUD01 Rpmandincrement:20~300rpm; 1rpm MotorType:Brushlessmotor Numberofroller:1 4. 5. FluorescentLampcontrolIncluded4setsofT5FluorescentLampandaexternalTimeron/offcontrol(24hr) ORPprobe:Gelfilledelectrode;Autoclavable

UtilityRequirement
1. 2. 3. 4. Buildingmainsupply110V60Hzor220V50Hzwithelectricalsafetycutoffswitch, Autoclaveforsterilization CoolingCirculatingWaterBathforcoolingpurpose CompressorAirtosupplyair,whichcomeswithareducertocontrolpressuretoaround1kg/cm2.Thisairistobe dry,oilfreeandfiltered. 5. Computerforremovecontrol

Thermostatsystem

AirLifter

Vessel

Type Max.workingvolume Totalvolume Material Controlpanel StorageProgram Controlunit Datastorage Cabinetmaterial Dimension Ratedvoltage Aeration Tube Sparger Controlsystem Temperature Range Probe Controlmode

Jacket

Singlewall (Notemp.controlsystem) 5.28Liter 6.6Liter

Borosilicateglass/316Lssforheadplateandallfittings 10.4ColorTouchscreenInterface Upto59,994programfordifferentkindsofcondition Upto10datafiles ABSfrontpanelandpaintedironhousing Footprint:400x500mm(WxD);Height:740mm 110Vor220V;50/60Hz Adjustabledrafttube Microsparger Thermostatsystem; Builtinheatexchanger(400Wheater/watercirculation pump);Automaticcoolingwatervalve 5abovecoolantupto60 PlatinumRTDprobe(Pt100) Programmable15stepsPIDcontroller

Communicationport RemotecontrolthroughEthernet(SCADA)

Datastorageinterface USBport

InletGasFlowmeter 110LPM

Range pH Probe Controlmode Range Probe DO Controlmode

212pH Gelfilledelectrode;Autoclavable Onepointcontrol 0200% PolarographicDOsensor;Autoclavable PID;Cascadefunctiontoresponseto a. Steppingincreasingordecreasingagitationspeed b. OxygenEnrichmentDevice(optionalitem) c. Tostartsubstratefeedingprogram. /+2000mV Gelfilledelectrode;Autoclavable(optional) 316LstainlesssteelwithinsulatedTeflontube; On/Offcontroller PreciseSteppingmotor;min.speedis1rpm

ORP Foam

Transmitter Probe Probe

FourbuiltinEasytubeloadingpumps;Oneexternalpump(optional) Peristaltic pump Speedrange Controlmode Exhaust

OptionalItem:
1. 2. OxygenEnrichmentDevice Oneexternalpump Specification: Model#:MUD01 Rpmandincrement:20~300rpm; 1rpm MotorType:Brushlessmotor Numberofroller:1 3. 4. FluorescentLampcontrolIncluded4setsofT5FluorescentLampandaexternalTimeron/offcontrol(24hr) ORPprobe:Gelfilledelectrode;Autoclavable

065rpm Programmable15stepsfeedingcontrol; PumpcanbeassignedforAcid,Base,Antifoamand Substrate 316LStainlesssteelcondenser

UtilityRequirement
1. 2. 3. 4. Buildingmainsupply110V60Hzor220V50Hzwithelectricalsafetycutoffswitch, Autoclaveforsterilization CoolingCirculatingWaterBathforcoolingpurpose CompressorAirtosupplyair,whichcomeswithareducertocontrolpressuretoaround1kg/cm2.Thisairistobe

 dry,oilfreeandfiltered. 5. Computerforremovecontrol