Code No: RR312302 Set No.

1
III B.Tech I Semester Supplimentary Examinations, November 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Explain the role of DNA binding proteins in Gene regulation. [8+8]

2. Write notes on any two:

(a) Tissue specific enhancers

(b) Phosphorylated proteins
(c) TATA box [8+8]

3. Explain the application of plasmids in genetic engineering. [4+12]

4. Write notes on any two:

(a) M13 vectors

(b) Cosmids
(c) PUC8 [8+8]

5. Give a brief definition of a gene library. What is the essential difference between
a genomic library and a cDNA library? List the major advantages/limitations on
the use of each. [8+8]

6. Discuss the application of PCR technology. [6+10]

7. Discuss the methods available for testing drug action and toxicity. [8+8]

8. Explain the importance of gene cloning in agriculture. [4+12]

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Code No: RR312302 Set No. 2
III B.Tech I Semester Supplimentary Examinations, November 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write notes on any two.

(a) Terminator loops

(b) Leader peptide
(c) Trp. Repressor [8+8]

2. Explain the expression of hormone regulated genes in eukaryotes [6+10]

3. Explain in detail transposable elements in prokaryotes. [4+12]

4. Write notes on any two:

(a) Shot gun cloning

(b) Cohesive end ligation
(c) Adenoviral vectors [8+8]

5. Write the steps involved in construction of a genomic library. [4+12]

6. Discuss about Multiplex PCR. [4+12]

7. Discuss the application of gene chips in various fields of Biotechnology. [4+12]

8. From improved agriculture point of view, how do transgenic plants play a vital
role? [4+12]

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Code No: RR312302 Set No. 3
III B.Tech I Semester Supplimentary Examinations, November 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write notes on any two

(a) C-Protein
(b) DNA binding proteins
(c) β- galactosidase [8+8]
2. Write notes on any two:
(a) Steriod hormonal regulation
(b) Short repeats in DNA
(c) DNA binding motifs. [8+8]
3. Write notes on any two:
(a) Mechanism of transpositions
(b) Isolation of plasmids
(c) Ty elements in yeast. [8+8]
4. Write notes on any two:
(a) Restriction digestion
(b) Homopolymer tailing
(c) Ligases [8+8]
5. Write short notes on any two:
(a) Southern blotting.
(b) Colony hybridization.
(c) Radioactive labeling. [8+8]
6. Write about the contents of a reaction mixture for PCR analysis. [6+10]
7. Discuss the methods available for testing drug action and toxicity. [8+8]
8. From improved agriculture point of view, how do transgenic plants play a vital
role? [4+12]

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Code No: RR312302 Set No. 4
III B.Tech I Semester Supplimentary Examinations, November 2007
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Presence of ‘glucose’ “switches off” the lac operon -justify the statement. [6+10]

2. Explain the role of peptide hormones in eukaryotic gene regulation. [4+12]

3. Write notes on any two:

(a) Inverted repeats

(b) Transposase
(c) ‘P’ elements [8+8]

4. Give the detailed methods of gene transfer in living cells. [4+12]

5. Explain the different types of probes and write about the steps involved in their
synthesis. [6+10]

6. How are PCR products identified? [4+12]

7. How does molecular markers find its application in gene discovery? [4+12]

8. Differentiate between In vivo and Ex vivo gene therapy. [8+8]

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