CHEE4020 Course Profile

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CHEE4020: Bioprocess Engineering A

School of Engineering
http://www.eng.uq.edu.au 2 Units, Internal, St Lucia Handbook description: Biochemical principles, enzyme reactions, kinetics, industrial applications, immobilisation. Microbial metabolism & kinetics. Fermentation processes & fermentor design & operational principles. Sterilisation, scale-up considerations. Biotechnological solid-liquid separations, cell disruption, product concentration & purification operations & design; recombinant protein recovery, product stabilistaion & formulation safety. Dr Matt Hardin matth@cheque.uq.edu.au

1. Course Details
1.1 Summary Semesters Offered: Semester 1, Incompatible: CHEE7302 or E1481 or 496 or 868 or 881, Pre-requisite: Permission of Head of School + (1E201 or BIOL2005 or BL205 or CHEE2001) 1.2 Introduction This course applies chemical engineering principles to biological processes. It includes enzyme reactions, kinetics immobilisation and industrial applications. A brief explanation of microbiology and microbial kinetics is given to allow analysis of fermentation processes, fermentor design & operational principles including sterilisation and scale-up considerations. Downstream processing of biologically derived products is examined including biotechnological solid-liquid separations, cell disruption, product concentration & purification operations and design, recombinant protein recovery, product stabilisation & formulation safety. It is of importance to any engineer working in the large scale bioprocessing area 1.3 Contact
Type Lecture Lecture Tutorial Location 63-358 68-212 50-C203 When Tuesday 8-9 Thursday 10-12 Friday 2-4 Notes

1.4 Laboratory Safety Students MUST read the University's policy (2.30.14) on Occupational Health and Safety in the Laboratory (Undergraduate Student). Students must complete the "Declaration Form for Practical Class Work" and return to the coordinator before the commencement of practicals. This policy and the declaration form can be accessed at http://www.uq.edu.au/hupp/contents/view.asp?s1=2&s2=30&s3=14

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2. Aims & Objectives

2.1 Course Aims This course applies chemical engineering principles to biological processes. It includes enzyme reactions, kinetics immobilisation and industrial applications. A brief explanation of microbiology and microbial kinetics is given to allow analysis of fermentation processes, fermentor design & operational principles including sterilisation and scale-up considerations. Downstream processing of biologically derived products is examined including biotechnological solid-liquid separations, cell disruption, product concentration & purification operations and design, recombinant protein recovery, product stabilisation & formulation safety. It is of importance to any engineer working in the large scale bioprocessing area 2.2 Learning Objectives After successfully completing this course you will be able to: Appreciate Specifics of biological processes: Clearly understand the differences between biological and traditional chemical processing unit operations and processes Asess Literature: Critically assess relevant literature in bioprocessing and draw parallels between described research and other industrial and research applications Design equipment: Apply basic design and operation considerations to fermentation, sterilisation and downstream processing equipment. Understand Biology Fundamentals: Display a fundamental understanding of the basics of biochemistry, microbial growth, enzyme kinetics and biotechnology 2.3 Course Content Biology: Cell structure and function, some useful bacteria proteins and enzymes, basic molecular biology Downstream Processing: Analytical techniques, flocculation, filtration, centrifugation, cell disruption, membrane operations, chromatography, solvent extraction, protein precipitation, recombinant proteins, inclusion bodies, crystallisation, drying Fermentation technology: Bioreactor design & operation, microbial kinetics, sterilisation & disinfection, mixing and aeration, heating and cooling.

3. Graduate Attributes
A. IN-DEPTH KNOWLEDGE OF THE FIELD OF STUDY
A1. A comprehensive and well-founded knowledge of the field of study. : Fundamental knowledge of the application of process engineering principles to biological systems taught through lectures, tutorials and labs and assessed by report, assignment and examination A3. An international perspective on the field of study. : Appreciation of the international and diverse nature of bioprocessing by study of relevant journal articles and web sites of international bioprocessing organisations

B. EFFECTIVE COMMUNICATION
B1. The ability to collect, analyse and organise information and ideas and to convey those ideas clearly and fluently, in both written and spoken forms. : Analysis of literature, web sites and practical results communicated in oral and written reports B2. The ability to interact effectively with others in order to work towards a common outcome. : Sudents will work togerther in teams to produce a coherent case study of one aspect of bioprocessing B4. The ability to engage effectively and appropriately with information and communication technologies. : The use of journals, library resources texts and the web will be required to complete the assessment.

C. INDEPENDENCE AND CREATIVITY

C1. The ability to work and learn independently. : Most assessment will be of an individual nature and will be tailored to ensure that idependent work is required. C3. The ability to identify problems, create solutions, innovate and improve current practices. : Basic knowledge will be delivered as part of the teaching materialkbut assessment will reuiqre the integration of this knowledge into new and previously unseen situations.

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D. CRITICAL JUDGEMENT
D2. The ability to apply critical reasoning to issues through independent thought and informed judgement : A critical review of literature is a central part of the assessment. D3. The ability to evaluate opinions, make decisions and to reflect critically on the justifications for decisions. : A critical review of literature is a central part of the assessment.

E. ETHICAL AND SOCIAL UNDERSTANDING

E1. An understanding of social and civic responsibility : Specific lectures on the ethical issues associated with biotechnology will be given as well as there being a requirement for comment on ethical issues within the context of the literature review

4. Assumed Background
The srtudent is assumed to have a firm grasp of the ropinciples of chemical enginerring including heat and mass transfer, reaction kinetics and communication skills. A grounding in biology is helpful but not essential.

5. Teaching & Learning Methods

Field Trip The student will visit a bioprocessing plant and gain an appeciation firsthand of the requirements for bioprocessing on an industrial scale Lecture The student will listen to prepared lectures and integrate the information into the context of their current knowledge Tutorial The student will practise skills taught in the lecture and gain an opportunity to more closely question teaching staff on doubtful points

6. Recommended Texts
6. 1 Recommended Texts Doran, P.M., 1995. Bioprocess Engineering Principles, 1st Edition, Academic Press, 6. 2 References Bailey, J.E. & Ollis , D.F., 1986. Biochemical engineering fundamentals, 2nd edition, McGraw-Hill, New York ; London ; Sydney . (Call Number:TP248.3 .B34 1986 ) Shuler, M.L. and Kargi, F. , 2001. Bioprocess Engineering: Basic Concepts, 2nd Edition, Prentice Hall,

7. Resources Available and Required

7.1 Resources Required and Other Resources Available(Including additional costs) Available: Library, web, handouts Required: Transport to field trip 7.2 Online Resources OHPS from lectures: http:// web site is not yet active, details to follow in class 7.3 Course Materials

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Files:
A. Cantarutti: cantarutti.pdf Presentation on 30-4-04 A. Mendes: mendes.pdf Presentation on 30-4-04 A. Rowlands: rowlands.pdf Presented 14/5/04 Assignment 1: assignment 1.doc due 26/3/04 counts as 1 of the 4 from 9 worth 5%. Assignment 2: assignment 2 sterilisation and analytical techniques.doc due 2/4/04 counts as 1 of the 4 from 9 worth 5%. Assignment 3: assignment 3 balances and kinetics.doc Due 23/4/04 counts as 1 of the 4 from 9 worth 5%. Assignment 4: assignment 4 mixing and aeration.doc Due 30/4/04 counts as 1 of the 4 from 9 worth 5%. Assignment 5 : assignment 4 filtration.doc Due 7/5/04 counts as 1 of the 4 from 9 worth 5%. B. Bhensdadia: bhensdadia.pdf Preseantion on 26/3/04 F. Tan: tan.pdf Presenattion on 7-5-04 Heating and Cooling: lecture 12 - heating and cooling.ppt Industry Case Study: industry case study.doc J-A Smith: smith.pdf presentation on 26/3/04 Lecture 1 - 2004: lecture 1-04 - introduction.ppt Lecture 11: lecture 11 - design and operation of fermentors.ppt Lecture 13 Aeration and Mixing: lecture 13 - mixing and aeration.ppt Lecture 14 Solids separation: filtration and centrifugation.ppt Lecture 15 Cell Disruption: lecture 14 cell disruption.ppt Lecture 16 membrane Operations: lecture 16 - membrane operations.ppt Lecture 2 2004: lecture 2 cell types and parts.ppt Lecture 3 2004: lecture 3 some useful bacteria.ppt Lecture 4 and 5 - 2004: lecture 3-4 2004 dna, proteins and enzymes.ppt Lecture 5-6 18/3/2004: lecture 5-6 2004 introduction to molecular biology and metabolic engineering.ppt Lecture 7 - 2004 Sterilsation and Disinfection: lecture 7 sterilisation and disinfection.ppt Lecture 8 - 2004 Analytical techniques: lecture 8 analytical techniques in bioprocessing.ppt Lecture 9-10 Kinetics and Balances: lecture 9-10 kinetics.ppt updated Thursday 1/4/04

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Literature review: literature review.doc M. Burns: burns.pdf Presentation on 2/4/04 Metabolic engineering paper : 34.pdf - essential for lecture 18/3 N. Lubach: lubach.pdf Presentation on 23-4-04 Presentation Dates: date for literature review presentation.doc

8. Assessment
8.1a Assessment Summary
Assessment Industry Case Study Due Friday Week 4 22-28 Mar 04 TBA Friday Recurring N/A Recurring Weighting Learning Objectives

15

Appreciate Specifics of biological processes, Asess Literature

Final Exam

50

Appreciate Specifics of biological processes, Asess Literature, Design equipment, Understand Biology Fundamentals Appreciate Specifics of biological processes, Asess Literature, Design equipment, Understand Biology Fundamentals Appreciate Specifics of biological processes, Asess Literature, Understand Biology Fundamentals

Assignments

5% each (total 20%) 15 (written 10 and Oral 5)

Literature Review

8.1b Assessment Detail

Industry Case Study

Learning Objectives Assessed: Appreciate Specifics of biological processes, Asess Literature Weight: 15 Reference URL: http:// Due Date
Friday Week 4 22-28 Mar 04

The industry study is a group report, the aim of which is to build up a summary of different areas of industrial bioprocessing. The reports generated will be in a standard format and bound to allow students access to a ready reference for the bioprocess industry. The report will cover: Industry/product description (5%) History of industry (10%) Description of process including unit operations and unit processes, organism, substrate etc. (15%) Quality issues - how they are measured and ensured (15%) Environmental and social effects of industry (15%) Major players both regionally and globally (5%) Regulatory environment of industry (10%) Future directions for industry (15%) Roles of chemical (and/or environmental) engineers in industry (5%)

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Job opportunities for graduates within industry including contacts at major players (5%)

The report will be assessed on: Completeness Accuracy Depth Grammar and spelling Use of references 25% 25% 25% 15% 10%

Each section will be marked independently with the weighting shown above. Each group is to submit a cover sheet stating the major contributor to each section. Marks will be allocated to the major contributor for each section as per the criteria above. Sources must be cited consistently and correctly, please see the library web site for correct citation methods. A variety of sources must be used including (but not limited to) industry publications, textbooks, reference books, technical books, journal articles, company annual reports and web. Submission: This assignment must be handed into the school of Engineering office by 4pm on the due date. To create a coversheet go to the online version of the course profile at www.eng.uq.edu.au. Marking Sheet: casestudy marksheet.doc

Final Exam
Learning Objectives Assessed: Appreciate Specifics of biological processes, Asess Literature, Design equipment, Understand Biology Fundamentals Weight: 50 Reference URL: http:// The end of semester examination will test content recall, problem solving and the the ability to extend the principles of the subject to hitherto unexplained situations. Due Date
TBA -

Assignments
Learning Objectives Assessed: Appreciate Specifics of biological processes, Asess Literature, Design equipment, Understand Biology Fundamentals Weight: 5% each (total 20%) Reference URL: http:// These assignments will consist of problem style questions involving practical principles described in lectures. They will typically consist of 2 questions. A single mark will result from each item. There will be eight assignments given out of which four must be completed. Each assignment will be worth 5% of the total marks for the course. In the event that more than four assignments are submitted the marks for the best four will be taken. Submission: This assignment must be handed into the school of Engineering office by 4pm on the due date. To create a coversheet go to the online version of the course profile at www.eng.uq.edu.au. Due Date
Friday Recurring -

Literature Review

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Learning Objectives Assessed: Appreciate Specifics of biological processes, Asess Literature, Understand Biology Fundamentals Weight: 15 (written 10 and Oral 5) Reference URL: http://

Due Date
N/A Recurring -

Find a journal article from a peer reviewed journal relevant to some aspect of downstream processing in biotechnology. The paper must be available as a PDF through the library. The student is to summarise the article and review the article especially with a view to the following: innovations shown by the work limitations of the work ethical implications of the work (if any) application of the work to areas outside the one explicitly mentioned possible future work that could follow from this paper

This activity has two main purposes, to help practise oral reporting skills and to get you to think about different aspects of research papers and hence get the most out of your reading. The paper must be approved by me by the Friday of the week before the presentation. I will make a copy of the PDF available on the web by the time of the Thursday lecture. The report will be given at then start of the tutorial session and the class will be encouraged to discuss the paper and the review. The written report is due at the same time as the oral.

FAILURE TO MAKE THE PAPER AVAILABLE FOR APPROVAL WILL CONSTITUTE A LATE SUBMISSION AND BE PENALISED 20%.
Marking Sheet: literature review marksheet.doc 8.2 Course Grading Policy Grade 7: Outstanding knowledge of fundamentals, clear precise, correct reasoning and excellent critical analysis of previously unseen problems. Grade 6: Excellent knowledge of fundamentals, clear precise reasoning and good critical analysis of previously unseen problems. Grade 5: Good knowledge of fundamentals, clear reasoning and fair critical analysis of previously unseen problems. Grade 4: Fair knowledge of fundamentals, clear reasoning and average critical analysis of previously unseen problems. Grade 3: Adequate knowledge of fundamentals, limited critical assessment of unseen problems. Grade 2: Poor knowledge of fundamentals, no critical analysis. Grade 1: Limited knowledge of fundamentals. Other Comments: 8.3 Late Submission Late submissions without prior consultation with the lecturer will attract penalties. The first working day will incur a penalty of 10% of the total mark for the assessment (not 10% of the students mark), the second day late will incur a penalty of a further 20% of the total mark, the third day 30%. Submissions received after this will not be marked.

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9. Plagiarism
Although students are encouraged to discuss amongst themselves and with their lecturers the concepts and ideas being presented in this course, any material submitted for assessment must be the student's own work. Copying or plagiarism (presenting as your own the ideas of others without proper acknowledgment or referencing) and falsifying results are forms of cheating and are unacceptable behaviour. This type of behaviour falls under the definition of misconduct in the University of Queensland Statute No 4 (Student Discipline and Misconduct) 1999. Students found copying or cheating or assisting a fellow student to cheat will receive penalties specified in Statute 4. Penalties may include loss of marks or credit for this course or you may be required to do extra or repeat work in the course.

10. Support for Students with a Disability

Any student with a disability who may require alternative academic arrangements in the course/program is encouraged to seek advice at the commencement of the semester from a Disability Adviser at Student Support Services.

11. Employment Screening

No employment screening is required for this course.

12. Dictionaries at Examinations

Dictionaries will be available to students during examinations.

13. Feedback
13.1 Course Feedback Students will be given timely feedback on all progressive assessment and broad feedback on end of semester exams. Students are encouraged to make the most of progressive feedback, it is a students responsibility to incorporate this feedback into their learning. Students may make an appointment for individual feedback with the coordinator. Students may request to see their end of semester exams. It is the student's responsibility to be aware of the rules, policies and other documents related to assessment; and providing teachers with feedback on their assessment practices. 13.2 Curriculum, Assessment and Teaching and Learning Feedback Students are encouraged to give feedback on their courses, both for future development and possible immediate changes in the course. To assist this process students have several options: 1. Make an appointment with the coordinator to discuss their concerns. 2. Use the TEDI anonymous feed back system. You can access the system at http://www.tedi.uq.edu.au/af/ . Your coordinator should have given you the username and password. 3. Your coordinator is encouraged to hold regular feedback sessions in class through out the semester. You can raise your concerns then. 4. If you are unable to resolve issues through the above procedures, the University has a standard policy for dealing with grievances. See HUPP

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14. Contact Schedule

Semester 1 Week Week 1 Week 1 Week 1 Week 2 Week 2 Week 2 Week 3 Week 3 Week 3 Week 4 Week 4 Week 4 Week 5 Week 5 Week 5 Week 6 Week 6 Week 7 Week 7 Week 7 Week 8 Week 8 Week 8 Week 9 Week 9 Week 9 Week 10 Week 10 Week 10 Week 11 Week 11 Week 11 Week 12 1-7 Mar 04 1-7 Mar 04 1-7 Mar 04 8-14 Mar 04 8-14 Mar 04 8-14 Mar 04 15-21 Mar 04 15-21 Mar 04 15-21 Mar 04 22-28 Mar 04 22-28 Mar 04 22-28 Mar 04 29-4 Apr 04 29-4 Apr 04 29-4 Apr 04 5-8 Apr 04 5-8 Apr 04 19-25 Apr 04 19-25 Apr 04 19-25 Apr 04 26-2 May 04 26-2 May 04 26-2 May 04 3-9 May 04 3-9 May 04 3-9 May 04 10-16 May 04 10-16 May 04 10-16 May 04 17-23 May 04 17-23 May 04 17-23 May 04 24-30 May 04 Day Tuesday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Thursday Friday Thursday Activity Lecture: 8-9 Intro to bioprocessing Lecture: 10-12 Cell structure and function Tutorial: Library Workshop Lecture: 10-11 Cell structure and function Lecture: 11-12 Some useful bacteria Tutorial: 2-4 Lecture: 10-11 Proteins and enzymes Lecture: 11-12 Basic molecular biology Tutorial: 2-4 Lecture: 10-11 Analytical techniques Lecture: 11-12 Sterilisation & disinfection Tutorial: 2-4 Lecture: 11-12 Microbial Kinetics I Lecture: 10-11 Heating and Cooling Tutorial: 2-4 Lecture: 10-11 Microbial Kinetics II Lecture: 11-12 Bioreactor Design and OPeration Lecture: 10-11 Mixing and Aeration Lecture: 11-12 Filtration Tutorial: 2-4 Lecture: 10-11 Centrifugation Lecture: 11-12 Cell Disruption Tutorial: 2-4 Lecture: 10-11 Membrane Operations Lecture: 11-12 Chromatography I Tutorial: 2-4 Lecture: 10-11 Chromatography II Lecture: 11-12 Solvent Extraction Tutorial: 2-4 Lecture: 11-12 Drying Lecture: 10-11 Flocculation and Precipitation Tutorial: 2-4 Lecture: 10-11 Crystallisation

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Week 12 Week 12 Week 13 Week 13 Week 13

24-30 May 04 24-30 May 04 31-6 Jun 04 31-6 Jun 04 31-6 Jun 04

Thursday Friday Thursday Thursday Friday

Lecture: 11-12 Drying Tutorial: 2-4 Lecture: 10-11 Recombinant Proteins Lecture: 11-12 Inclusion Bodies Tutorial: 2-4

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