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Phosphate salts are known by several names and the correct phosphate must be used to prepare buffer solutions. One phosphate cannot be substituted for another phosphate. Check formula of salt to be certain.
Formula
Name of salt
Other names
KH2PO4
potassium dihydrogen orthophosphate monobasic potassium phosphate potassium dihydrogen monopotassium phosphate phosphate acid potassium phosphate potassium biphosphate dipotassium hydrogen orthophosphate potassium hydrogen dipotassium hydrogen phosphate phosphate dibasic potassium phosphate dipotassium phosphate potassium phosphate tribasic potassium phosphate tripotassium phosphate
K2HPO4
K3PO4
Standardization buffers
For pH=7.00 : Add 29.1 ml of 0.1 molar NaOH to 50 ml 0.1 molar potassium dihydrogen phosphate. Alternatively : Dissolve 1.20g of sodium dihydrogen phosphate and 0.885g of disidium hydrogen phosphate in 1 liter volume distilled water. For pH= 4.00 : Add 0.1 ml of 0.1 molar NaOH to 50 ml of 0.1 molar potassium hydrogen phthalate . Alternatively : Dissolve 8.954g of disodium hydrogen phosphste.12 H2O and 3.4023g of potassium dihydrogen phosphate in 1 liter volume distilled water.
Buffering system Hydrochloric acid/ Potassium chloride Glycine/ Hydrochloric acid Potassium hydrogen phthalate/ Hydrochloric acid Citric acid/ Sodium citrate Sodium acetate/ Acetic acid Potassium hydrogen phtaalate/ Sodium hydroxide Disodium hydrogen phthalate / Sodium dihydrogen orthophospate Dipotassium hydrogen phthalate / Potassium dihydrogen
Useful buffering pH range @ 25C 1.0 - 2.2 2.2 - 3.6 2.2 - 4.0 3.0 - 6.2 3.7 - 5.6 4.1 - 5.9
5.8 - 8.0
5.8 - 8.0
orthophospate Potassium dihydrogen orthophosphate / sodium hydroxide 5.8 - 8.00 Barbitone sodium / Hydrochloric acid 6.8 - 9.6
Tris (hydroxylmethyl) aminomethane / Hydrochloric acid 7.0 - 9.00 Sodium tetraborate/ Hydrochloric acid Glycine/ Sodium hydroxide Sodium carbonate/ Sodium hydrogen carbonate Sodium tetraborate/ Sodium hydroxide Sodium bicarbonate / Sodium hydroxide Sodium hydrogen orthophosphate / Sodium hydroxide Potassium chloride/ Sodium hydroxide 8.1 - 9.2 8.6 - 10.6 9.2 - 10.8 9.3 - 10.7 9.60 - 11.0 11.0 - 11.9 12.0 - 13.0
Preparing a Buffer Solution This page gives tabulated info on the preparation of buffers by mixing adjusters with a known volume of the primary salt solution, and made up to 200ml with distilled water. BUFFERS 1.00 - 9.00
Buffer B : pH 2.2 - 4.00 100 ml 0.1 M potassium hydrogen phthalate + mls of 0.1 M HCl. pH mls of 0.1M HCl added
Buffer C : pH 4.10 - 5.90 100 ml 0.1 M potassium hydrogen phthalate + mls of 0.1 M NaOH pH mls of 0.1M NaOH
Buffer D : pH 5.8 - 8.00 . 100 ml 0.1 M KH2PO4 + mls of 0.1 M NaOH. pH mls of 0.1M NaOH
Buffer E : pH 7.0 - 9.00 100 ml 0.1 M tris (hydroxymethyl) aminomethane + mls of 0.1 M HCl. pH mls of 0.1 M HCl added
1.00 1.10 1.20 1.30 1.40 1.50 1.60 1.70 1.80 1.90 2.00 2.10 2.20
added 134.0 105.6 85.0 67.2 53.2 41.4 32.4 26.0 20.4 16.2 13.0 10.2 7.8
2.20 2.30 2.40 2.50 2.60 2.70 2.80 2.90 3.00 3.10 3.20 3.30 3.40 3.50 3.60 3.70 3.80 3.90 4.00
99.0 91.6 84.4 77.6 70.8 64.2 57.8 51.4 44.6 37.6 31.4 25.8 20.8 16.4 12.6 9.0 5.8 2.8 0.2
4.10 4.20 4.30 4.40 4.50 4.60 4.70 4.80 4.90 5.00 5.10 5.20 5.30 5.40 5.50 5.60 5.70 5.80 5.90
added 2.6 6.0 9.4 13.2 17.4 22.2 27.2 33.0 38.8 45.2 51.0 57.6 63.2 68.2 73.2 77.6 81.2 84.6 87.4
5.80 5.90 6.00 6.10 6.20 6.30 6.40 6.50 6.60 6.70 6.80 6.90 7.00 7.10 7.20 7.30 7.40 7.50 7.60 7.70 7.80 7.90 8.00
added 7.2 9.2 11.2 13.6 16.2 19.4 23.2 27.8 32.8 38.6 44.8 51.8 58.2 64.2 69.4 74.0 78.2 82.2 85.6 88.4 90.6 92.2 93.4
7.00 7.10 7.20 7.30 7.40 7.50 7.60 7.70 7.80 7.90 8.00 8.l0 8.20 8.30 8.40 8.50 8.60 8.70 8.80 8.90 9.00
93.2 91.4 89.4 86.8 84.0 80.6 77.0 73.2 69.0 64.0 58.4 52.4 45.8 39.8 34.4 29.4 24.4 20.6 17.0 14.0 11.4
BUFFERS 08 - 13
Buffer H : pH 9.60 11.00 100 mL 0.025 M 100 mL 0.025 M 100 mL 0.05 Na2B4O7.10H2O Na2B4O7.10H2O M NaHCO3 + (borax) + mls of 0.1 (borax) + mls of 0.1 mls of 0.1 M M HCl. M NaOH. NaOH. pH mls of 0.1M pH mls of 0.1M pH mls of
Buffer I : Buffer J : pH 10.90 pH 12.00 12.00 13.00 100 mL 0.05 50 mL 0.2 M M Na2HPO4 + KCl + volume mls of 0.1 M indicated (in NaOH. mL) 0.2 M NaOH. pH mls of pH mls of
HCl added
NaOH added
8.00 8.10 8.20 8.30 8.40 8.50 8.60 8.70 8.80 8.90 9.00 9.10
41.0 39.4 37.6 35.4 33.2 30.4 27.0 23.2 19.2 14.2 9.2 4.0
9.20 9.30 9.40 9.50 9.60 9.70 9.80 9.90 10.00 10.10 10.20 10.30 10.40 10.50 10.60 10.70 10.80
1.8 7.2 12.4 17.6 22.2 26.2 30.0 33.4 36.6 39.0 41.0 42.6 44.2 45.4 46.6 47.6 48.5
9.60 9.70 9.80 9.90 10.00 10.10 10.20 10.30 10.40 10.50 10.60 10.70 10.80 10.90 11.00
0.1M NaOH added 10.0 12.4 15.2 18.2 21.4 24.4 27.6 30.4 33.0 35.6 38.2 40.4 42.4 44.0 45.4
10.90 11.00 11.10 11.20 11.30 11.40 11.50 11.60 11.70 11.80 11.90 12.00
0.1M NaOH added 6.6 8.2 10.2 12.6 15.2 18.2 22.2 27.0 32.4 38.8 46.0 53.8
12.00 12.10 12.20 12.30 12.40 12.50 12.60 12.70 12.80 12.90 13.00
0.2M NaOH added 12.0 16.0 20.4 25.6 32.4 40.8 51.2 64.4 82.4 106.0 132.0
Make up the following solutions (1) 0.1M acetic acid (2) 0.1M sodium acetate (tri-hydrate) (13.6g / l) Mix in the following proportions to get the required ph
ph 3 4 5 6
vol. of 0.1M acetic acid 982.3 mls 847.0 mls 357.0 mls 52.2 mls
vol. of 0.1M sodium acetate 17.7 mls 153.0 mls 643.0 mls 947.8 mls
10 4.77g sodium tetraborate and 183ml of 0.10M NaOH 11 2.10g sodium bicarbonate and 227ml of 0.10M NaOH
1. The Physical and Theoretical Laboratory, Oxford University. 2. "Electrolyte solutions" Robinson, R. A., and Stokes, R. H., 2nd ed., rev. London, Butterworths, 1968. 3. "Practical chemistry" J. Lambert and T.A. Muir, 3rd. Ed. Heineman, London. 4. pdf file, www.bc.ca/bcsc/resources/ (Canadian Teachers Federation).
Signature: Dhanlal De Lloyd, Chem. Dept, The University of The West Indies, St. Augustine campus The Republic of Trinidad and Tobago. Copyright: delloyd2000 All rights reserved.