m-ColiBlue24 Broth

The best value for coliform detection in drinking water

EPA Accept ed
40 C Parts 1 FR 41, 14 3

EPA accepted Easy to detect and identify coliforms and Escherichia coli No confirmation required

Detection and Identification Within 24 Hours m-ColiBlue24 broth simultaneously detects and identifies total coliforms and Escherichia coli, so there is no need for a confirmation step. Easy to Read m-ColiBlue24 broth makes it easy to differentiate between coliforms and E. coli: E. coli are blue; other coliforms are red; total coliforms are the sum of the two. Its that simple. Easy to Interpret With m-ColiBlue24 broth, there are no color keys, no fluorescent lamps. You interpret results directly from the plate. Enumerative Potable drinking water should be free from coliform bacteria or E. coli in a 100 mL sample. If you discover there are coliforms in the water, you will want to know the extent of the problem. With m-ColiBlue24 broth, you can get a coliform and/or E. coli count and take the appropriate action immediately.

Less Expensive m-ColiBlue24 broth is considerably less expensive than other detection methods. In addition, Millipore not only offers the total solution, but also special discount packages on media, Petri dishes, membranes, and filtration equipment. Backed by Millipore Technical Support The Millipore Technical Services department provides technical support and acts as a resource for regulatory information. Applications m-ColiBlue24 broth has been approved by the EPA for monitoring drinking water using a 24-hour incubation period. It can also be used to detect coliforms in other types of waterbottled, surface, ground, well, recreational (fresh or marine), as well as process water for ultrapure chemical processing.

D A T A

S H E E T

m-ColiBlue24 Broth Gives You More for Your Money

Enzymatic Substrate Coliform Test m-ColiBlue24 Broth

Next-day results Detects total coliforms and E.coli No confirmation step required Direct interpretation Quantitative Less expensive*

X X X

X X X X X X

*Comparison based on list prices as of 2003 of m-ColiBlue24 broth (Millipores price) and a leading enzymatic substrate coliform test.

Using m-ColiBlue24 Broth A standard volume of 100 mL is tested for drinking water with the expectation of finding zero coliform or E. coli colonies. When analyzing samples from other sources, it may be necessary to test larger or smaller volumes. The goal is to obtain samples that produce approximately 50 coliform colonies and not more than 200 colonies of all types per membrane. Procedure 1. Mix the broth by inverting the ampoules two or three times. Pour the contents evenly over the absorbent pad and place the lid on the Petri dish. 2. Filter sample or diluted sample using a 47 mm white gridded 0.45 m membrane filter, grid side up. Transfer the filter to the previously prepared Petri dish using sterile forceps. 3. Place the filter grid side up, onto the absorbent pad with a slight rolling motion. Check for trapped

air under the filter and make sure the filter touches the entire pad. Replace the Petri dish lid. 4. Invert the Petri dish and incubate at 35 0.5 C for 24 hours. 5. Count the colonies. Use a 10 to 15X stereoscopic microscope if necessary. If no red or blue colonies appear, the sample can be considered negative. If red or blue colonies appear at 24 hours incubation, the result is positive for total coliforms or E. coli respectively. No confirmation step is required. Calculating Coliform Density Use the following equation to calculate coliform density on a single membrane filter: Coliforms per 100 mL = Coliform colonies counted x 100 mL sample filtered Note: mL sample refers to actual sample volume, and not volume of the dilution.

Evaluating and Reporting Results If growth covers the entire filtration area of the membrane or a portion of it, and colonies are not discrete, report results as Confluent Growth With or Without Coliforms. If the total number of colonies (coliforms plus non-coliforms) exceeds 200 per membrane or the colonies are too indistinct for accurate counting, report the results as Too Numerous To Count (TNTC). In either case, you must run a new sample using a dilution that provides about 50 coliform colonies and not more than 200 colonies of all types.

To Place an Order or Receive Technical Assistance For additional information call your nearest Millipore office: In the U.S. and Canada, call toll-free 1-800-MILLIPORE (1-800-645-5476) In the U.S., Canada and Puerto Rico, fax orders to1-800-MILLIFX (1-800-645-5439) Outside North America contact your local office. To find the office nearest you visit www.millipore.com/offices. Internet: www.millipore.com Technical Service: www.millipore.com/techservice Also Available From Fisher Scientific in U.S. and Canada To place an order call: 1-800-766-7000

Specifications
Incubation Sensitivity Selectivity Storage and Shelf Life QC Panel

24 hours at 35 0.5 C 1 CFU/100 mL Contains inhibitors to minimize the growth of non-coliform organisms Refrigerate at 2 to 8 C for up to one year from date of manufacture Enterobacter cloacae (ATCC# 23355) Escherichia coli (ATCC# 25922) Klebsiella pneumoniae (ATCC# 13883) Pseudomonas aeruginosa (ATCC# 27853)

Ordering Information
Description Catalogue No.

m-ColiBlue24 Broth, plastic ampoules, 2 mL, 50/pk

M00P MCB 24

Millipore is a registered trademark of Millipore Corporation. m-ColiBlue24 is a registered trademark of Hach Company, U.S. patents 5,650,290 and 5,849,515. Lit. No. PF196EN00 Rev. A 06/03 03-138 2000, 2003 Millipore Corporation, Billerica, MA. All rights reserved.