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Code No: B0301 JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD M.Tech II - Semester Regular Examinations September, 2010 MICROBIAL BIOREACTORS ENGINEERING (Biotechnology) Time: 3hours Max. Marks: 60 Answer any five questions All questions carry equal marks --1.a) Write short note on: i) Yield ii) Selectivity. A batch Fermentor was operated for the production of alcohol from yeast. The rate of glucose conversion is measured and the data are presented below. Find the time taken in the batch fermentor when the concentration falls down to 12 kg mol/m3 when CA0 = 70 kg mol/m3 68 2.82 54.6 4.71 33.0 4.31 20.0 3.74 12.2 3.16

b)

Concentration (CA)kg mol/m3 Rate of reaction(-rA) kg mol/m3 h 2.

What is kLa ,What are the different methods available to measure kLa. Compare and contrast the static method and dynamic method for the measurement of kLa. Write about the effect of the following on oxygen transfer rate a) Bubble size b) Gas hold up c) Gas velocity d) Type of Gas sparger. Explain the theory of Power requirement for ungassed Newtonean fluids with neat diagram of Reynolds number and Power number for various impellers A fermentation broth containing a dilute pullalans culture is agitated in a 5 lit reactor containing rushton turbine impeller. The vessel geometry containing four baffles is as per the one described by Rushton. The impeller diameter is 16 cm and is rotating at 300 rpm. The apparent viscosity of the fluid is 8 cp and its density is 1010 kg/m3. Find the power required to run the agitator using the plots of power number versus Reynolds number. Describe the importance of mixing in bio processing. What are the different types of agitators used in bio processing? Mention five physical parameters to be monitored and explain in detail how they are monitored in the fermentation process? Contd -2-

3.

4.a) b)

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7.4 2.46

4.5 1.91

2.7 1.4

5.a) b) 6.

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-27. After a batch fermentation, the system is dismantled and approximately 75% of the cell mass is suspended in the liquid phase(2l), while 25% is attached to the reactor walls and internals in a thick film(0.3 cm). Work with radio active tracers shows that 50% of the target product (intracellular) is associated with each cell fraction. The productivity of this reactor is 2 g product/liter at the 2 l scale. What would be the productivity at 20,000 l scale if both reactors has a height to diameter ratio of 2 to 1? Compare various methods for the aeration of animal cell cultures. Comment on the advantages and disadvantages of each method?

8.

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