animal and plant respiration purpose: to determine the oxygen demand in invertebrate animals and plants Basic Theory

Metabolic rate is the total amount of energy produced and used by the body per unit time. Metabolic rate is closely related to respiration since respiration is the process of extracting energy from food molecules are dependent on the presence of oxygen. Simply put, the chemical reactions that occur in respiration can be written as follows: C6H12O6 + 6O2 CO2 + 6H2O + 6 ATP Metabolic rate is usually estimated by measuring the amount of oxygen consumed per unit time living. This is possible because the oxidation of foodstuffs requires oxygen (in a known amount) to produce energy that can be known amount. However, the rate of metabolism is usually fairly expressed in the form of the rate of oxygen consumption. Several factors influence the rate of oxygen consumption, among others, temperature, animal species, body size, and activity. Oxygen consumption rate can be determined in various ways, including by using mikrorespirometer, Winkler method, and Scholander respirometer. The use of each method is based on the type of animal to be measured the oxygen consumption rate. Mikrorespirometer used to measure oxygen consumption of small animals such as insects or spiders. Winkler method is a way to determine the amount of oxygen dissolved in water . In this method, the levels of oxygen in water is determined by titration. Titration is the addition of a solution of known concentration (standard solution) into another solution of unknown concentration gradually until equilibrium. Scholander respirometer used to measure the rate of oxygen consumption of animals such as frogs or mice. This tool consists of a syringe, manometer, tube specimen and control tube. tool: simple respirometer analytical scales pipette drops vaseline tweezers eosin / methylen blue material: insects (crickets) KOH crystals mung bean sprouts tissue, cotton

way of working: 1. crystal wrap KOH / NaOH with a cotton swab, then insert a tube into the respirometer. 2. weigh insects (crickets) to be tested 3. close the tube with pipe-scale respirometer 4. oleskanlahvaselin on the cover of the tube slit 5. scale pipe end cap with a finger about 1 minute, then remove and insert the eosin / ink on the end of the pipe with a dropper, to facilitate, then place the respirometer is tilted on the table, while the position of the pipette parallel to table surface 6. observe the movement of liquid ink, record the distance traveled during a certain time 7. do in animals (crickets) and plants (sprouts) observational data: No materials testing Weight ( gr ) much need for oxygen ( ml / dt ) 1. crickets 1,2 gr @30 a. 0,2 b. 0,35 c. 0,48 d. 0,55 e. 0,65 2. sprouts 3 gr @60 a. 0,02 b. 0,05 c. 1,2 result of the implementation of the table 1. Crickets = 0,2 + 0,15 + 0,13 + 0,07 + 0,1 = 0,13 = 0,004333 = 0,00361 ml/dt/gr 5 30 detik 1,2 gr 2. sprouts = 0,002 + 0,003 + 1,15 = 0,4 = 0,0067 = 0,00222 ml/dt/gr 3 60 detik 3 gr Discussion: In this experiment, especially in experiments using respirometer, used KOH. Function of the solution is to bind CO2, so that the movement of Brodie solution is really only due to oxygen consumption. The reaction that occurs between the KOH with CO 2 is as follows: KOH + H2O + CO2 K2CO3 In addition to KOH, Brodie solution is also an important component. Brodie is a component of a solution of NAI, stergent, and Evan's blue. Nai is a difficult compound to react, so it will not arise deviation data obtained. Stergent a detergent-like compounds that cause the movement of Brodie solution along the capillary tube becomes easy because the surface tension becomes small. Evan's blue is a compound that causes the blue Brodie solution. conclusion we can know that the oxygen demand in invertebrate animals more than the oxygen demand on the plant. Moreover, the respiration rate of plant respiration hewan.the weight also influent the object of research.