Fator de Crescimento: So mediadores biolgicos naturais que atuam sobre os processos de reparo e regenerao.

. Encontrados em vrios tecidos em fase de cicatrizao.

Processo de Manufatura do Fator de Crescimento

Signal Sequence Growth Factor Gene

cDNA

Ultra Filtration Gene Cloning Secreted growth factors Fermentation

Column

Vetor

Purification

Transformation
Vector

Purified Growth factors

E. coli

A Caregen vem desenvolvendo dezenas de diferentes tipos de peptdeos, citoquinas, fator de crescimento, hormnios, neurotransmissores, imunomoduladores, como, EGF, IGF-1, bFGF, IL-10 e ainda fatores de crescimento semelhantes aos peptdeos humanos, como o Rejuline e CG-IDP2, todos com diversas atividades comparadas aos hormnios de crescimento intactos.

A importncia dos peptdedos e do fator de crescimento na regulao da expresso gentica e do metabolismo celular estabelecida na biologia da pele.

Funes dos peptdeos e fatores de crescimento:

Remove clulas epidermais danificadas; Estimula a proliferao celular na pele e folculos capilares; Repara e previne rugas alm de minimizar as cicatrizes na pele; Reduz e previne a celulite; Previne a queda capilar; Revitaliza e nutre as clulas epidermais e do couro cabeludo.

A Caregen lder mundial em cosmecutica A vantagem destes peptdeos como ingredientes cosmticos so: Ao prolongada, devido a alta estabilidade, resultado da modificao da seqncia primria; Penetrao transdrmica por nano estrutura; Alta pureza do produto.

Fatores de crescimento:

Anti - Ageing
CG-EGF
(Epidermal Growth Factor)

Anti-Rugas
CG-EGF
(Epidermal Growth Factor)

CG-IGF-1
(Insulin-like Growth Factor1)

CG-IGF-1
(Insulin-like growth factor1)

CG-bFGF
(basic Fibroblast G.F.)

CG-bFGF
(basic Fibroblast G.F.)

CG-aFGF
(acid Fibroblast G.F.)

CG-aFGF
(acid Fibroblast G.F.)

CG-TRX
(Thioredoxin)

CG-KGF
(Keratinocyte G.F.)

CG-Somatotropin
(Human Growth Factor)

CG-TGF-3
(Transforming G.F. 3)

CG-Thymosin 4
Etc..

CG-Thymosin 4
Etc..

CG-EGF Fator de Crescimento Epidermal

INCI name: Human Oligopeptide-1
Reduz

e previne linhas e rugas pela ativao de novas clulas da pele; Devolve a uniformidade no tom da pele, devolvendo vitalidade e energia; Recupera a aparncia jovial da pele;
Elimina cicatrizes e manchas da pele.

Conc. de uso: 1 5%

CG-IGF 1 Fator de Crescimento Isulin Like - 1

INCI name: Human Oligopeptide-2
Melhora

a aparncias de linhas e rugas de expresso; Aumenta a produo de colgeno e elastina da pele e reduz manchas avermelhadas; Possui um efeito redutor de gordura (drenagem) facial e corporal;
Estimula os folculos capilares a produzirem um cabelo mais denso e forte.

Conc. de uso: 1 5%

Efficacy Test Report

EGF IGF-1 EGF+IGF-1

EGF and IGF-1 increase cell proliferat in to relation to ECM composition.

12
Fold Increase In [3H] Thimidine Incorporation

10 8 6 4 2 0

PL

Col

Col

FN

LN

ECM (Extracellular Matrix) Molecules

CG-bFGF
Fator de Crescimento Fibroblstico bsico
INCI name: Human Oligopeptide-3
Reduz

e previne linhas e rugas pela ativao de novas clulas da pele;; Rejuvenecedor da pele, repara cicatriz e escoriaoes; Melhora a elasticidade da pele;
Melhorara a circulao perifrica, sendo indicado para desordens capilares.

Conc. de uso: 1 5%

Rejuline
INCI name: Acetyl Decapeptide-3 Reduz e previne as linhas atravs da atividade das novas clulas formadas; Repara e melhora a cicatrizao de ferimentos; Fortalece e modula a elasticidade da pele, por induo da sntese de colgeno e elastina; Regula a sntese de colgeno; Ao mais estvel e prolongada que as protenas nativas. Conc. de uso: 1 5%

6. Apoptosis retardation by IDP2 & Rejuline

Control

CG-IDP2

Rejuline

The cell-cycle analysis and apoptotic retardation in human HacaT cell by peptides - Cell line : Human keratinocyte ( HacaT Cell) / Starvation : 24hrs / Peptide treatment : 72hrs - Control, IDP2 & Rejuline: 5 umole, Vitamin C: 5 umole - ProG1(M1) indicates the phase of apoptosis by FACS.

Rejuline age no incremento da sntese de pr colgeno Haca T (Queratincito humano)

35 30 25
21.1

Control

Rejuline (1 ug/ml)

30.2

20

Procolagen (ng/ml)

15 10 5 0 1 Day 2 Day 3 Day

3.8 9.9 7.8 2.5 8.3 4.2 5.0

12.3 6.9 5.8

4 Day

5 Day

6 Day

HacaT Human Keratinocytes in 2% serum condition Procollagen ELISA Kit analysis; Rejuline Peptide (1 ug/ml) treatment

Aumento da sntese de cido hialurnico CCD986SK (Fibroblasto Humano Cell Line)

210 176

cido Hialurnico (ng/ml)

170

159

130

117

90

75.7

50

Controle

IDP-2

Rejuline

Vit. C 50ppm

Clulas fibroblasticas humanas em serum condition Hyaluronic Acid ELISA Kit analysis; 5umole of each peptides treatment for 72 hrs

Efficacy Test Report Cell (NIH3T3) Test

Rejuline is modulates the skin elasticity by regulation of collagenase. B. Rejuline Treated A. Control

Aps 72hs de incubao com Rejuline 5ng/ml numa codio serum free.

Efficacy Test Report

- Tested by Ellead Antes Aps 4 semanas

Efficacy Test Report

- Tested by Ellead Antes Aps 4 semanas

Mechanism of Growth Factors in Skin

Mecanismo do Fator de Crescimento na pele

Fibrin clot

Epidermis

Neutrophil Platelet plug

Macrophag e

TGF

Dermis

Blood vessel VEG F

FGF VEG PDGF F BB PTGF-1 PDGF AB

IGF KGF PDG F

Fat

Neutrophil Macrophag e FGF2

FGF-2

Fibroblast

TGF1

Manufacturing Process of Growth Factors

Nano-Capsulation

Topical Delivered System : Nano-capsulation

IDP 2 have almost full activities compared to their intact growth factors.

Efficacy Test Report -Mouse (C3H) Test

Efficacy Test Report

Ingredients Registered on CTFA -As of July 14, 2006

EGF

EGF ; Epidermal Growth Factor

Efficacy Test Report

III) In vitro test 3

IV) In vitro test 4

Anti-hair loss complex solution have an additive effect on keratinocyte proliferation

A

B: 7 Days later

D: 7days later

Mouse Skin Culture: A,B: Control C,D: Complex treatment

III. IGF-1 can induce Cell proliferation and Epidermis Thickness

1) Cell growth assay (SRB) 2) IGF-1 induces Epidermal Thickness
a) Control
cel pr ier i (HacaT) l olf aton 100 80 60 40 20 0 -20
control 100pg/m l 1ng/m l 10ng/m l 100ng/m l 1ug/m l

b) IGF-1 Treated(1ug/ml)

Outer layer Outer layer Epidermis

Epidermis dermis

dermis

I F-1 G

IGF-1 induced cell proliferation in Human Keratinocyte cells(Hacat) as a dose dependant manner which was observed with SRB Assay.

Nano-capsulayted IGF-1 induced epidermis thickness after 1 week treatment in mouse skin.

V) In vitro test 5: Mouse Wound Healing 4 days later

Control

Copper Peptide

Control (HA only) Hair Complex

IGF-1 ; Insulin-like Growth Factor-1

IGF -1

Efficacy Test Report

bFGF ; basic Fibroblast Growth Factor

bFGF

bFGF modulates theskin elasticity by regulation of collagenase.

(ng/ml)

Rejuline

Rejuline

Rejuline is modulates the skin elasticityby regulation of collagenase

VI) In vitro test 6: Mouse Hair Growth test

Hair growth stimulation (Mouse C3H / HeN, 4month Old)
After 1day Control After 14days After 25days

Black Skin

Red Skin

After 1day Anti-hair loss complex

After 14days

After 25days

Black Skin

Red Skin

Clinical test for Healthier hair and scalp_ # 1

# 1. Before

After (1weeks)

After (2weeks)

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