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Reactordesignforenzyme catalyzedreactions

BatchReactor
dS k 2 E0 S k E 2 0 dt K m S K m 1 S S ln( 0 ) S S0 K m ln( ) S 0 S k 2 E0t S 0 S S S ln( 0 ) S 1 k 2 E0 t S0 S K m K m (S0 S )
FindtheratelawconstantsVm andKM usingintegral method

t S0 S

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OtherFormats
K m ln( S0 ) S 0 S k 2 E0 t S

1 K m ln( ) S 0 X k 2 E0 t 1 X P S0 S S0 ) P k 2 E0 t S0 P

K m ln(

1 K m ln( ) S 0 X k 2 E0 t 1 X
If Km<< S0, zero order reaction

S0 X k 2 E0

If Km>> S0, first order reaction

K m ln(1 X ) k 2 E0t

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CSTR
v0 S 0 v0 S rSV v0 ( S 0 S ) k 2 E0 S V Km S
If Km<< S0, zero order reaction

S0 X k 2 E0

k ES (S0 S ) 2 0 Km S k 2 E0 S 0 X K m X 1 X

If Km>> S0, first order reaction

Km X k 2 E0 1 X

HowaboutPFR?

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Idealenzymereactor
Factorsthatneededtobeconsidered:

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BatchReactor Goodforsmallscale experimentalstudy Useofsoluble enzyme Operational convenience Canuseinsoluble substrate

CSTR
EasytodopHcontrol Colloidial orinsoluble substratecanbe processed Easyreplacementof catalyst Higheryieldforreaction undergoingsubstrate inhibition

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CSTRmembrane
1. 2. Abilitytouseandrecycle solubleenzyme Enzymelossdueto adsorptiononthe membrane Concentration polarizationofthe enzymeatthe membranesurface Poorstabilityforthe enzymeforlongterm operation

3.

4. Enzyme recycle product

PackedBedReactor
Higherconversion (comparedtoCSTR) Higheryieldfor reactionundergoing productinhibition

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FluidizedBedReactor
1. Betterheatandmass transfer 2. Insolublesubstratecan beused 3. Lowpressuredrop 4. Largepower requirementsforfluiding thebed 5. Uncertaintyinscalingup