NAME: SCHOOL: SEROLOGY QUESTIONNAIRE 100 POINTS I.

IDENTIFICATION NO ABBREVIATION PLS 25 POINTS

DATE:

1. _____________________________ is the specific recognition and combination of antigen with antibody 2. Tumor marker for pancreatic cancer: __________________________________________________________ 3. Primary hypothyroidism: ____________________________________________________________________ 4. Unit used for ASO rapid slide latex: __________________________________________________________ 5. The common substrate used for the enzyme peroxidise? _________________________________________________ 6. In radioimmunoassay, the sold crystal gamma counter is used to quantify ___________________________________ emissions 7. A heterophile antibody test for rickettsia: _________________________________________________________ 8. Autoimmune disease due to impaired T cell production: _______________________________________________ 9. Also known as progressive systemic sclerosis: _______________________________________________ 10. What is the source of the enzyme alkaline phosphatise? _______________________________________________ 11. It is the pH at which proteins exist in an ampholyte state? _______________________________________________ 12. During paratyphoid fever antibody titer reaches its peak within how many weeks? _______________________________________________ 13. _____________________________ is the conformation of amino acid chain resulting from inter chain hydrogen bonding 14. In dean webb or alpha titration technique, there is an excess of ________________________ during the prozone phase 15. During agglutination reaction, the involved particulate antigen is called _________________________ 16. What is the enzyme produced from E. coli?___________________________________ 17. What is the ANTIBODY class used for the neutralization of endotoxin? ________________________ 18. Significant titer for vaccinated persons for widal? ______________________________ 19. Flagellar antigen: ___________________________________ 20. ANA pattern that is seen in progressive systemic sclerosis and sjogrens syndrome? ____________________________________________ 21. Also known as heavy chain disease? _________________________________________________________ 22. Causes hyperthyroidism due to thyroid stimulating immunoglobulin: ____________________________________________

23. Autoimmune disease caused by a defect in the maturation of B cells: ____________________________________________ 24. Highly associated to the presence of immunoglobulin light chains in the urine: ____________________________________________ 25. Breast cancer associated antigen: _____________________________________________

II.

ENUMERATION 13 POINTS 1. 5 points. For heterogenous RIA give 5 methods that may result to the precipitation of proteins/separation step

2. 4 points: Give 4 methods used to determine Rheumatoid factor in rheumatoid arthritis

3. 4 POINTS: Give 4 types of hypersensitivity

III.

MORSE TYPE OF EXAM (2 points each) 32 points Select if: A. If one is correct B. If two are correct C. If three are correct D. If four are correct E. If NONE is correct 1. For serologic application of the principle of precipitation are: a) CRP DETERMINATION b) ASCOLI RING TEST c) CRP d) TESTING FOR ADULTERATED FOOD 2. In heterophile antibody test for IM: a) In Forsman Ab: with GPK, antibodies are absorbed. Hence no agglutination with sRBC b) In serum sickness: with GPK, antibodies are not absorbed. Hence agglutination with sRBC. c) In IM: with GPK, antibodies are not absorbed. Hence agglutination with sRBC. d) In serum sickness: with BE, antibodies are not absorbed. Hence agglutination with sRBC. 3. Application of RICE test: a) TREPONEMA PALLIDUM CF b) REITER PROTEIN CF c) WASSERMAN-KOLMER CF d) BLASTOGENESIS CF 4. Rules in the performance of febrile agglutinin test: a) Tests are preferably done every 6-12 days b) Definite diagnosis is by 2-3 days c) Antibodies are occasionally formed by related infectious groups d) Battery test is not advocated for brucella and rickettsia spp. 5. Diseases caused by Rickettsiae typhus group: a) BRILL ZINSER DSE b) BOUTONNEUSE FEVER c) MURINE /ENDEMIC d) SCRUB TYPHUS 6. Using the rantz-randall test for ASO titer determination: a) The red cell control must not display hemolysis b) The erythrocyte suspension is composed of human O RBC at 5% suspension c) The Streptolysin O ctrl must display complete hemolysis d) The erythrocyte suspension is composed of rabbit erythrocytes

7. In electrophoresis, movement is dependent on: a) THE TAGGING BY NON-SEPARATION OF BOUND FROM FREE FRACTION b) DEGREE OF IONIZATION OF PROTEIN AT pH OF BUFFER SOLUTION c) THE USE OF ORGANIC SOLVENTS d) THE USE OF ABSORPTION MEDIA 8. Causative agent/s for rickettsia spotted fever group: a) R. rickettsi b) R. tsutsugamishi c) R. prowazeki d) R. akari 9. In radial immunodiffusion, consider the following: a) POLYMERIC Ig DIFFUSE SLOWLY AND THUS MAY LEAD TO UNDERESTIMATION b) HIGH MOL. WEIGHT IMMUNE COMPLEXES RESULT TO FALSELY LOW VALUES c) FALSE HIGH VALUES FOR LOW MOLECULAR WEIGHT Ig d) REVERSE PRECIPITATION MAY OCCUR IN CONDITIONS WHERE THE TESTED SERUM CONTAINS ANTI-Ig 10. Application of indirect immunofluorescence a) FADF b) FITC c) DANSYL d) BAIF 11. Serodiagnostic test for Myco. Pneumonia: a) CFT b) EIA c) PCR d) DIRECT IF 12. Microbial features of B-hemolytic group A streptococci: a) Erythrogenic toxins b) L-fucosyl transferase c) Hyaluronidase d) Galactosyl transferase 13. In the WIDAL test rapid slide method: a) Makes use of the kline slide b) Rotates at 180 rpm for 5 minutes c) Gently shake the commercially prepared salmonella antigens to ensure heterozygous suspension d) A serum volume of 0.08 mL is approximately a 1:20 dilution

14. Serologic features of Myco. pneumonia: a) Cold hemagglutinins b) Heterophile antibody production which cross reacts with capsular polysaccharide of Strep. Pyo group c) Cross reactive antigens d) Allo antibody production 15. For Q fever: a) The area of occurrence is Europe, Africa b) The etiologic agent is Coxiella burnetti c) Its reservoir hosts are mites and rodents d) Its mode of transmission is via recrudescence 16. For cold hemagglutinins in Prim. Atyp. Pneumonia a) It appears during the 1st week of infection b) It reaches its peaks on 1st to 5th week of infection c) Declines to low levels on the 7th week IV. MODIFIED TRUE OR FALSE (3 POINTS EACH) 30 POINTS If TRUE, write TRUE. If FALSE, write the word FALSE followed by the wrong word, and followed by the right word to be placed. EXAMPLE: A. Sir Jon Paul Reyes is the chief medical technologist of DMMC laboratory TRUE B. Maam Kate Faminialagao, is the section head of clinical chemistry FALSE WRONG WORD/S= CLINICAL CHEMISTRY RIGHT WORD/S=SEROLOGY AND IMMUNOLOGY

1. During agglutination, the initial sensitization phase occurs followed by lattice formation

2. During precipitation sensitization occurs in the area of optimal ratio between antigen and antibody

3. In CFT the antigen control contains the antibody and its end result is lysis

4. In CFT, the haemolytic system control is positive for the antigen and the antibody hence lysis is the end result

5. Double diffusion-single dimension gel precipitation test by Oakley and fulthrope determines the number of antigen and antibody in the specimen

6. For cold hemagglutinin test for PAP, the highest dilution that shows a 2+ result is considered a positive result.

7. In Ehrlichioses, the mode of transmission for E. canis is the lone star tick bite.

8. In tularaemia infection, antibodies usually appear within 3-5 weeks.

9. AFP is a major fetal protein that resembles globulin.

10. CA 125 is a high molecular weight purine- like glycoprotein useful for non mucinous epithelial ovarian cancer

LIFE IS LIKE A BOX OF CHOCOLATES, YOU DON'T KNOW WHATS IN IT UNTIL YOU OPEN IT GOOD LUCK INTERNS! BY: KATE FAMINIALAGAO