Chem 5 quiz bank Chem Ch5 test bank 1. Tandem mass spectrometers a.

[are two mass spectrometers placed in series with each other] 2. Which of the following is not an objective of proteomics research? a. [Identifying specific gene mutations] 3. Which of the following is true concerning fluorometry? a. [ fluorescence is an inherently more sensitive technique than absorption 4. Which of the following refers to the movement of buffer ions and solvent relative to the fixed support? a. [electrodosmosis] 5. Which of the following techniques has the highest potential sensitivity? a. [chemiluminescence] 6. What is the purpose of the chopper in an atomic absorption spectrophotometer? a. [ correct for the amount of light emitted by the flame] 7. Which of the following procedures is not currently or routinely used for point of care testing devices ? a. [Polymerase Chain reaction] b. Colometric detection c. Biosensors d. Immunochromatography e. Electrical detection 8. Which of the following correctly ranks electromagnetic radiation from low energy to high energy? a. [microwaves, infrared, visible, UV, x-rays, gama, cosmic] 9. Which electrochemical assay measures current as fixed potential? a. [amperometry] 10. Which is the most sensitive detector for spectrophotometry? a. [photomultiplier] 11. Which of the following light sources is used in atomic adsorption spectrophotometry? a. [hollow cathode lamp] 12. Which of the following is beers law? a. [A=*b*c] 13. Which of the following best describes the process of fluorescence? a. [molecules emit a photon at lower energy when excited electrons return to ground state] 14. Which of the following regarding mass spectrometry is FALSE? a. Mass spectrometry detects for gas and liquid chromatography [true] b. Mass spectrometers can be used to sequence DNA[false] c. Ions are formed by the bombardment of electrons[true] d. Each chemical compound has a unique mass spectrum[true]

Chem 5 quiz bank e. Quadruple and ion trap sectors separate ions according to their mass to charge ratio[true] Reverse phase liquid chromatography refers to [ a polar mobile phase and a nonpolar stationary stage] Which of the following is NOT an advantage of capillary electrophoresis a. Cations, neutrals, and anions move in the same direction at different rates b. Very small sample size c. Rapid analysis d. Use of traditional detectors e. Multiple samples can be assayed simultaneously on one injection [not advantageous] Stray light in a spectrophotometer places limits on a. [upper range of linearity] Which is the most accurate concerning ion selective electrodes? a. [gas specific membranes are necessary for oxygen and carbon dioxide electrodes] Which of the following is FALSE concerning the principles of point of care testing devices? a. Whole blood analysis is the preferred specimen[true] b. Onboard microcomputers control the instrument functions and data reduction[true] c. Devices do not require quality control testing[false] d. Biosensors have enabled miniaturization particularly amendable for POC testing[true] e. They use principles that are identical to laboratory-based instrumentation[true] Which of the following is NOT necessary for obtaining the spectrum of a compound from 190=500nm? a. A double-beam spectrophotometer b. Tungsten light source [not necessary] c. Photomultiplier d. Quartz cuvettes e. Deuterium light source

15. 16.

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20.