Spectra/Por

Dialysis membrane tubing, discs, sheets and accessories

Three types of Dialysis Membranes Regenerated Cellulose (RC) Cellulose Ester (CE) Polyvinylidene Difluoride (PVDF)

17 Choices of Molecular Weight Cut Offs From 100 to 1.000.000 Daltons

Two different Qualities Regular Quality Membranes Biotech Quality Available

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Irradiated and/or Non-irradiated

Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

Introduction dialysis
Dialysis is the process of diffusion of solutes through a semi permeable membrane from a liquid with a higher solute concentration on one side of the membrane to a liquid with a lower concentration on the other side. The membranes are semi permeable because they allow some molecules to pass while preventing others from passing. The process has long been used for molecular separation of small molecules from macromolecules. This relatively simple process is cost effective for several reasons: a. Wide Range of Sample Volumes b. Gentle Conditions c. Inexpensive Membrane and Equipment

Common dialysis applications utilize tubular forms of membranes and involve placing a sample inside the membrane and a buffer outside the membrane. The process is run until the desired degree of separation is attained. Molecules smaller than the pores will eventually be equally distributed between the two solutions. Usually, a very large volume of buffer is chosen so that the permeable species are greatly diluted and therefore reduced to very small concentrations in the remaining sample solution. Commonly, dialysis processes require several hours to complete. The original type of dialysis membranes utilized for diffusion studies was cellulose nitrate. These membranes were not ideal because they tended to have high protein adsorption, thus limiting their use in the purification and separation of biological solutions. Regenerated cellulose membranes were then used because they alleviated the protein adsorption problems. Regenerated cellulose also exhibits good chemical and temperature resistance. The membranes, however, were limited in Molecular Weight Cut Off (MWCO). Greater membrane permeability was achieved through the use of cellulose ester. These solutions could be formulated to yield a wider range of pore sizes. Cellulose Ester membranes are now widely used for clinical and laboratory dialysis. Membranes used for dialysis have pore sizes ranging from 100 to 100.000 Daltons. For simple dialysis applications of removing salts or small molecules, the use of membrane tubing is convenient, efficient and inexpensive. This can be accomplished with a length of membrane tubing in a container with buffer. Stirring the buffer is recommended to increase the dialysis rate. Dialysis closures are used to provide an easy method for sealing the ends of the dialysis tubing.

How to select Molecular Weight Cut Off (MWCO)? Dialysis membrane pore sizes are characterized by the molecular weight at which 90% of the solute will be retained (preventive from permeating) by the membrane. The permeability of a solute is depending upon the shape of the molecule, its degree of hydration and its charge. Each of these may be influenced by nature of the solvent, the pH and the ionic strength. As a general rule, it is best to choose a MWCO that is half the molecular weight of the solute to be retained. For effective separation, please refer to the following formula as a guide: MW retained MW passed 25

This maximizes the sample recovery and minimizes the time required for dialysis.

Dialysis using conventional tubular membranes

Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

Dialysis Procedure
The following dialysis procedure is a general protocol for basic dialysis. There are many variables that should be taken into consideration before starting the dialysis of your sample. Some of the variables that will affect the rate of dialysis are sample solute concentration, sample solute size, solute binding, sample solvent, temperature, etc. Therefore, some application specific changes to the following dialysis procedures may be necessary. Dialysis Reservoir
Fill a Spectra/Por dialysis reservoir (e.g. the Spectra/Por Dialysis Reservoir) with a large volume of your dialysate of choice. Most dialysis applications require that your dialysate consists of the same components as your dialysis sample except for the components (solutes and/or solvents) that you want to remove from your sample. Ideally, the dialysate volume should equal 100x your sample volume. (Example: dialyse 10 ml of sample in a liter of dialysate). Drop a clean magnetic stir bar into the dialysis reservoir. Make sure that the stir bar is large enough to stir the entire dialysate volume but not too large that it cannot freely rotate. Place the dialysis reservoir on a stirrer. After you place your dialysis sample in the dialysis reservoir, adjust the control for the maximum speed that does not pull down your sample by the vortex.

least 2 mm wider than the flat width of the membrane. Clamp the closure shut and locked over the tubing end. Make sure that the end of the membrane tubing is still extending squarely past the closure. Note: Spectra/Por CE Closures (nylon) can be used with all types of membrane tubing. Spectra/Por Closures (polypropylene) can only be used with Regenerated Cellulose membranes. (see Closures on pages 22-23-24). Holding the other end of the membrane tubing upright and open, load your sample, keeping in mind the volume and the tubing length. Hold the tubing upright; carefully clamp the second closure on the top end of the tubing piece, allowing for a headspace. Make sure that the membrane extends squarely past the closure after clamping. Gently pinch along the length of the tubing to detect any leaks. If leaks are found, the closure needs to be reapplied.

Dialysis
Place the membrane tubing loaded with your sample into the dialysis reservoir containing the dialysate solution. If the membrane does not float, either remove some sample volume or reapply the closures to increase the headspace in the membrane tubing. Adjust the speed control on the stirrer high enough to stir the entire volume of dialysate but not high enough to pull the sample down in the vortex. Place the lid on the dialysis reservoir to prevent dialysate evaporation. Typically, dialysis is allowed to run overnight. During the duration of dialysis, the entire dialysate volume should be changed for fresh dialysate solution at least three times. Recommended dialysate changes are at 2-4 hours, 6-8 hours, and 10-14 hours (next morning). Dialysis should be allowed to continue for at least 2 hours after the last dialysate change. Note: For highly concentrated samples, you may need to dialyse for a longer duration with more frequent dialysate changes. The temperature of dialysis primarily depends on the sample. There are temperature limits depending on the membrane type being used. Cellulose Ester (CE) can withstand temperatures up to 37 C. Regenerated Cellulose (RC) can withstand temperatures up to 60 C. PVDF can withstand temperatures up to 130 C. After a sufficient period of time has passed to bring dialysis to completion, turn off the stirrer and remove the membrane tubing containing the sample from the dialysis reservoir. Holding the tubing by one end, carefully remove the upper membrane closure and remove the sample with a plastic pipette and dispense into an appropriate container.

Loading Dialysis Membrane with Sample

Cut off the appropriate length of dialysis membrane that you will need for your sample. To determine the length of tubing you need refer to the Volume/Length ratio (ml/cm) provided on the membrane packaging or in the Spectrum Catalog. You will need to cut a piece long enough that will allow for your entire volume plus 10% of sample volume for head space (air that keeps the sample buoyant) plus at least two cm for each closure (4 cm totally). Note: If membrane tubing has not been previously prepared, the cut piece may need to be prepared depending on the application requirements. Open one end of the prepared tubing piece using your fingers. Work the rest of the tubing piece open. If the membrane is dry and not prepared, use a Spectra/Por Opener. Fold the Opener so that the adhesive sections are exactly opposite of each other. Remove the brown adhesive protection strips. Align the edge of the Opener with the end of the dry tubing such that the membrane tubing is laying across the opener. Fold the Opener over the tubing, press hard and hold on the adhesive portion of the Opener for about five seconds. Now gently and slowly pull the two halves of the Opener apart. The adhesive portions on the Opener should pull the end of the membrane tubing open. Lay one end of the membrane tubing across an open membrane closure, such that there is at least 1 cm extending past the open closure. Make sure that the gripping portion of the closure is at

Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

MWCO list
100 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) 500 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) 1.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 2.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE)

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Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

The Spectra/Por Dialysis Membranes from SPECTRUM have proven to be the most useful and most extended dialysis membrane product range available for laboratory dialysis:

3 TYPES OF DIALYSIS MEMBRANES:

Spectra/Por Regenerated Cellulose (RC) Dialysis Membranes. These membranes have a good chemical compatibility for selective organic solvents, a pH range of 2 12 and a temperature stability of up to 60 C. There are two types of RC membranes: - Spectra/Por Regenerated Cellulose - Standard Quality - Spectra/Por Biotech Regenerated Cellulose (ultra pure)

Spectra/Por Biotech Cellulose Ester (CE) Dialysis Membranes. The Cellulose Ester Membranes are ready-to-use membranes with a wide selection of MWCO ratings ranging from 100 to 100.000 Daltons. Biotech CE membranes are stable at pH 2 9 and have a temperature stability of up to 37 C. CE membranes, however, are not compatible with organic and strong polar solvents.

Spectra/Por Polyvinylidene Difluoride (PVDF) Dialysis Membranes. The PVDF Dialysis membranes are hydrophobic, ready-to-use membranes with an excellent chemical resistance. The membranes are resistant to a wide variety of organic solvents as well as most aqueous acids and bases, a pH range of 1 to 14 and a temperature stability of up to 130 C.

17 CHOICES OF MOLECULAR WEIGHT CUT OFFS (MWCO):

The Regenerated Cellulose Membranes have cut offs from 1.000 through 50.000 and the Cellulose Ester membranes have cut offs from 100 through 100.000 and the Polyvinylidene Difluoride membranes are available in 250.000 500.000 and 1 million Daltons.

IRRADIATED AND NON IRRADIATED:

Biotech Regenerated Cellulose (RC) membranes and Biotech Cellulose Ester (CE) Membranes are available irradiated (sterile).

Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

MWCO list
Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 3.500 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 3 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC)

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Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 3 Ready-to-Use Dialysis Sacks Regenerated Cellulose (RC) Spectra/Por 3 Dialysis Membrane Discs Regenerated Cellulose (RC) Spectra/Por 3 Dialysis Membrane Sheets Regenerated Cellulose (RC) 5.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) 6.000 8.000 Daltons Spectra/Por 1 Ready-to-Use Dialysis Sacks Regenerated Cellulose (RC)

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Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

MWCO list
Spectra/Por 1 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 1 Dialysis Membrane Discs Regenerated Cellulose (RC) Spectra/Por 1 Dialysis Membrane Sheets Regenerated Cellulose (RC) 8.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Regenerated Cellulose (RC) Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 10.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 12.000 14.000 Daltons Spectra/Por 2 Ready-to-Use Dialysis Sacks Regenerated Cellulose (RC)

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Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

MWCO list
Spectra/Por 4 Ready-to-Use Dialysis Sacks Regenerated Cellulose (RC) Spectra/Por 2 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 4 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 5 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 2 Dialysis Membrane Discs Regenerated Cellulose (RC) Spectra/Por 4 Dialysis Membrane Sheets Regenerated Cellulose (RC) Spectra/Por 5 Dialysis Membrane Sheets Regenerated Cellulose (RC) 15.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Regenerated Cellulose (RC)

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Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 25.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE)

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Spectra/Por Dialysis membrane tubing, discs, sheets and accessories

MWCO list
Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 50.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) 100.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Tubing Irradiated Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Discs Cellulose Ester (CE) Spectra/Por Biotech Dialysis Membrane Sheets Cellulose Ester (CE) 250.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Polyvinylidene Difluoride (PVDF) 500.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Polyvinylidene Difluoride (PVDF) 1.000.000 Daltons Spectra/Por Biotech Dialysis Membrane Tubing Polyvinylidene Difluoride (PVDF)

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Spectra/Por 1 Dialysis Membrane Tubing, Dialysis Sacks, Discs and Sheets. Regenerated Cellulose (RC)
MWCO (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging: Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Wall thickness sheets: Membrane storage: Dialysis Sacks storage: Shelf life: Closure recommendation: 6.000 8.000 2 12 60 C transparent, flexible dry (except Dialysis Sacks) glycerine heavy metals, sulfides 10 to 120 1 to 1.000 yes 30 50 m for flat width 10 80 mm 70 m for flat width 100 mm to 120 mm dry at room temperature or at 4 C in a polyethylene bag in original packing at 4 C approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 1 Dialysis Membrane Tubing 6.000 - 8.000 MWCO Regenerated Cellulose (RC) Product Number 132 645 132 650 132 665 132 660 132 665 132 670 132 675 Flat Width (mm) 10 23 32 40 50 100 120 Diameter (mm) 6,4 14,6 20,4 25,5 32,0 64,0 76,0 Vol/Length (ml/cm) 0,32 1,7 3,3 5,1 8,0 32,0 46,0 Length (meter) 15 30 30 30 30 15 15

10

Spectra/Por 1 Dialysis Membrane Dialysis Sacks 6.000 - 8.000 MWCO Regenerated Cellulose (RC) Product Number 132 651 132 666 Flat Width (mm) 23 50 Volume 1 - 40 ml 50 - 400 ml Length 60 cm 60 cm Package 10 10

Spectra/Por 1 Dialysis Membrane Discs 6.000 - 8.000 MWCO Regenerated Cellulose (RC) Product Number 132 478 132 476 132 474 Diameter (mm) 33 47 100 Package 50 50 50

Spectra/Por 1 Dialysis Membrane Sheets 6.000 - 8.000 MWCO Regenerated Cellulose (RC) Product Number 132 677 Dimension (mm) 240 x 240 Package 25

Spectra/Por 2 Dialysis Membrane Tubing, Dialysis Sacks, Discs and Sheets. Regenerated Cellulose (RC)
Spectra/Por 2 Dialysis membranes have the same MWCO as Spectra/Por 4 and 5. Spectra/Por 2 membranes however are much more porous than Spectra/Por 4 and 5. The wall thickness of Spectra/Por 2 differs from the wall thickness of Spectra/Por 4 and 5. MWCO (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging; Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Wall thickness sheets: Membrane storage: Dialysis Sacks storage: Shelf life: Closure recommendation: 12.000 14.000 2 12 60 C transparent, flexible dry glycerine (except Dialysis Sacks) heavy metals, sulfides 8 to 120 1 to 1.000 yes 20 50 m for flat width 6 45 mm 135 155 m for flat width 105 mm 120 mm dry at room temperature or at 4 C in a polyethylene bag in original packing at 4 C approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 2 Dialysis Membrane Tubing 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 685 132 676 132 678 132 680 132 682 132 684 Flat Width (mm) 6 10 25 45 105 120 Diameter (mm) 3,8 6,4 16,0 29,0 67,0 76,0 Vol/Length (ml/cm) 0,1 0,32 2,0 6,4 34,0 46,0 Length (meter) 15 15 15 15 15 15

11

Spectra/Por 2 Dialysis Membrane Dialysis Sacks 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 679 132 681 Flat Width (mm) 25 45 Volume 1 40 ml 50 400 ml Length 60 cm 60 cm Package 10 10

Spectra/Por 2 Dialysis Membrane Discs 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 482 132 480 132 477 Diameter (mm) 33 47 100 Package 50 50 50

Spectra/Por 2 Dialysis Membrane Sheets 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 686 Dimension (mm) 200 x 200 Package 25

Spectra/Por 3 Dialysis Membrane Tubing, Dialysis Sacks, Discs and Sheets. Regenerated Cellulose (RC)
MWCO (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging; Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Wall thickness sheets: Membrane storage: Dialysis Sacks storage: Shelf life: Closure recommendation: 3.500 2 - 12 60 C transparent, flexible dry glycerine (except Dialysis Sacks) heavy metals, sulfides 18 to 54 2 to 200 yes 25 - 30 m for flat width 18 - 54 mm 25 m for flat width 108 x 108 mm dry at room temperature or at 4 C in a polyethylene bag in original packing at 4 C approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 3 Dialysis Membrane Tubing 3.500 MWCO Regenerated Cellulose (RC) Product Number 132 720 132 724 132 725 Flat Width (mm) 18 45 54 Diameter (mm) 11,5 29,0 34,0 Vol/Length (ml/cm) 1,0 6,4 9,3 Length (meter) 15 15 15

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Spectra/Por 3 Dialysis Membrane Dialysis Sacks 3.500 MWCO Regenerated Cellulose (RC) Product Number 132 721 132 726 Flat Width (mm) 18 54 Volume 1 40 ml 50 400 ml Length 60 cm 60 cm Package 10 10

Spectra/Por 3 Dialysis Membrane Discs 3.500 MWCO Regenerated Cellulose (RC) Product Number 132 488 132 486 132 484 Diameter (mm) 33 47 100 Package 50 50 50

Spectra/Por 3 Dialysis Membrane Sheets 3.500 MWCO Regenerated Cellulose (RC) Product Number 132 723 Dimension (mm) 108 x 108 Package 25

Spectra/Por 4 Dialysis Membrane Tubing, Dialysis Sacks, Discs and Sheets. Regenerated Cellulose (RC)
Spectra/Por 4 Dialysis membranes have the same MWCO as Spectra/Por 2 and 5. Spectra/Por 4 membranes are less porous than Spectra/Por 2. The wall thickness of Spectra/Por 4 differs from the wall thickness of Spectra/Por 2. MWCO (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging: Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Wall thickness sheets: Membrane storage: Dialysis Sacks storage: Shelf life: Closure recommendation: 12.000 - 14.000 2 - 12 60 C transparent, flexible dry glycerine (except Dialysis Sacks) heavy metals, sulfides 10 to 75 1 to 300 yes 20 - 50 m for flat width 10 - 75 mm 40 m for flat width 150 x 150 mm dry at room temperature or at 4 C in a polyethylene bag in original packing at 4 C approximately two years depending on storages conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 4 Dialysis Membrane Tubing 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 697 132 700 132 703 132 706 132 709 Flat Width (mm) 10 25 32 45 75 Diameter (mm) 6,4 16,0 20,4 29,0 48,0 Vol/Length (ml/cm) 0,32 2,0 3,3 6,4 18,0 Length (meter) 30 30 30 30 15

13

Spectra/Por 4 Dialysis Membrane Dialysis Sacks 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 701 132 707 Flat Width (mm) 25 45 Volume 1 40 ml 50 400 ml Length 60 cm 60 cm Package 10 10

Spectra/Por 4 Dialysis Membrane Discs 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 498 132 496 132 494 Diameter (mm) 33 47 100 Package 50 50 50

Spectra/Por 4 Dialysis Membrane Sheets 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 712 Dimension (mm) 150 x 150 Package 25

Spectra/Por 5 Dialysis Membrane Tubing and Sheets Regenerated Cellulose (RC)

Spectra/Por 5 is reinforced with a layer of porous paper to increase its wet strength for use in high shear or torque environments. Spectra/Por 5 has a relatively low permeation rate for solutes compared to other membranes. MWCO (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging; Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Wall thickness sheets: Membrane storage: Shelf life: Closure recommendation: 12.000 14.000 2 12 60 C thick, rigid dry glycerine heavy metals, sulfides 65 to 140 25 to 1.200 yes, only as sheet 81 m for flat width 65 140 mm 81 m for flat width 275 x 275 mm dry at room temperature or at 4 C in a polyethylene bag approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 5 Dialysis Membrane Tubing High Wet Strength 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 754 132 757 Flat Width (mm) 75 140 Diameter (mm) 48,0 89,0 Vol/Length (ml/cm) 13,0 62,0 Length (meter) 15 15

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Spectra/Por 5 Dialysis Membrane Sheets 12.000 14.000 MWCO Regenerated Cellulose (RC) Product Number 132 759 Dimension (mm) 275 x 275 Package 25

Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC)

MWCO range (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging; Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Membrane storage: Shelf life: Closure recommendation: 1.000 2.000 3.500 8.000 10.000 - 15.000 25.000 50.000 2 12 60 C transparent, flexible wet 0,1% sodium azide heavy metals, sulfides 6 to 54 1 to 150 no 60 65 m for flat width 6 54 mm store wet membranes at 4 C in a preservative solution of 0,1% sodium azide approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC)

Spectra/Por 6 Dialysis Membrane Tubing Regenerated Cellulose (RC) Product Number MWCO 1.000 132 636 132 638 132 640 MWCO 2.000 132 620 132 625 132 633 MWCO 3.500 132 590 132 592 132 594 MWCO 8.000 128 056 132 579 128 058 132 580 132 582 132 584 132 586 MWCO 10.000 128 106 132 570 128 118 132 572 132 574 132 576 MWCO 15.000 128 156 132 560 128 158 132 562 132 564 132 566 MWCO 25.000 128 206 132 550 128 218 128 224 132 552 132 554 MWCO 50.000 132 539 132 540 132 542 132 544 Flat Width (mm) 18 38 45 Diameter (mm) 11,5 24 29 Vol./Length (ml/cm) 1,0 4,6 6,4 Length (meter) 10 10 10

18 38 45

11,5 24 29

1,0 4,6 6,4

10 10 10

18 45 54

11,5 29 34

1,0 6,4 9,3

10 10 10

6 12 18 24 32 40 50

3,8 7,5 11,5 15 20,4 25,5 32

0,1 0,45 1,0 1,8 3,3 5,1 8,0

10 10 10 10 10 10 10

6 12 18 24 32 45

3,8 7,5 11,5 15 20,4 29

0,1 0,45 1,0 1,8 3,3 6,4

10 10 10 10 10 10

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6 12 18 24 32 45

3,8 7,5 11,5 15 20,4 29

0,1 0,45 1,0 1,8 3,3 6,4

10 10 10 10 10 10

6 12 18 24 28 34

3,8 7,5 11,5 15 18 22

0,1 0,45 1,0 1,8 2,5 3,7

10 10 10 10 10 10

10 12 28 34

6,4 7,5 18 22

0,32 0,45 2,5 3,7

10 10 10 10

Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC)

Spectra/Por 7 Membranes have a minimum sulphur and heavy metal contamination. The membranes only have to be rinsed in DI water before use to remove the preservative. MWCO range (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging; Preservative: Trace elements contents: Flat width range (mm) Sample volume range (ml): Discs/flat sheets: Wall thickness tubing: Membrane storage: Shelf life: Closure recommendation: 1.000 2.000 3.500 8.000 10.000 - 15.000 25.000 50.000 2 12 60 C transparent, flexible wet 0,1% sodium azide minimal 6 to 54 1 to 150 no 60 65 m for flat width 6 54 mm store wet membranes at 4 C in a preservative solution of 0,1% sodium azide approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) Product Number MWCO 1.000 132 103 132 104 132 105 MWCO 2.000 132 107 132 108 132 109 MWCO 3.500 132 110 132 111 132 112 MWCO 8.000 128 356 132 113 128 358 132 114 132 115 132 116 132131 MWCO 10.000 128 406 132 117 128 418 132 118 132 119 132 120 Flat Width (mm) 18 38 45 Diameter (mm) 11,5 24 29 Vol/Length (ml/cm) 1,0 4,6 6,4 Length (meter) 5 5 5

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18 38 45

11,5 24 29

1,0 4,6 6,4

5 5 5

18 45 54

11,5 29 34

1,0 6,4 9,3

5 5 5

6 12 18 24 32 40 50

3,8 7,5 11,5 15 20,4 25,5 32

0,1 0,45 1,0 1,8 3,3 5,1 8,0

5 5 5 5 5 5 5

6 12 18 24 32 45

3,8 7,5 11,5 15 20,4 29

0,1 0,45 1,0 1,8 3,3 6,4

5 5 5 5 5 5

Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC)

Spectra/Por 7 Dialysis Membrane Tubing Regenerated Cellulose (RC) Product Number MWCO 15.000 128 456 132 121 128 458 132 122 132 123 132 124 MWCO 25.000 128 506 132 125 128 518 128 524 132 126 132 127 MWCO 50.000 132 128 132 129 132 130 Flat Width (mm) 6 12 18 24 32 45 Diameter (mm) 3,8 7,5 11,5 15 20,4 29 Vol./Length (ml/cm) 0,1 0,45 1,0 1,8 3,3 6,4 Length (meter) 5 5 5 5 5 5

6 12 18 24 28 34

3,8 7,5 11,5 15 18 22

0,1 0,45 1,0 1,8 2,5 3,7

5 5 5 5 5 5

12 28 34

7,5 18 22

0,45 2,5 3,7

5 5 5

Spectrum Easy-to-Use Dialysis Products

Spectrum has many readyto-use dialysis products that we have created to help customers save time and energy in dialyzing small volumes of samples. No longer need to prepare your own dialysis tubing, no boiling, no clamps and no knots but easily: There is a choice between a sterile (irradiated) and a non-sterile dialysis device for sample volumes from 300 l to 10 ml and a dialysis device for l-scale for sample volumes smaller than 100 l.

17

For Easy-to-Use products, simply ask for the Spectrum Easy-to-Use brochure.

1 Float-A-Lyzer

Ultra performance Easy-to-Use Spectra/Por Float-A-Lyzer increases choices for small sample volumes with MWCO selection from 100 to 100.000 Daltons.

OPEN
the dialysis device

RINSE
in distilled water
2 DispoDialyzer
The Ready-to-Use irradiated Spectra/Por DispoDialyzer eliminates membrane

LOAD
your sample
2

preparation for convenience in all dialysis applications.

3 Micro DispoDialyzer

um Spectr makes is Dialys ... easier

3

This dialysis device is specially designed for easy and effective dialysis of samples with a volume of 10 l, 50 l or 100 l.

Spectra/Por

Biotech Dialysis Membranes Regenerated Cellulose (RC)

The Spectra/Por Biotech Membranes are designed to fill the need for a Regenerated Cellulose (RC) dialysis membrane with a rigidly controlled retention rating. The Biotech RC Membranes offer exceptional freedom from impurities such as sulfides and metal contaminants which are commonly found in standard dialysis membranes. Supplied as non-sterile, dry membrane tubing. There are no special precleaning treatments required for Biotech RC Membranes. Spectra/Por Non-sterile Biotech RC membranes are supplied as a dry membrane with glycerine as a humectant in rolls of 15 meters.

MWCO range (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging: Packaging irradiated membranes: Trace elements contents: Flat width range (mm): Tubing diameter (mm): Vol./Length-ml/cm: Sample volume range (ml): Discs/flat sheets: Available irradiated (sterile): Membrane storage: Irradiated membrane storage: Shelf life: Closure recommendation:

3.500 8.000 10.000 15.000 25.000 2 12 60 C opaque, flexible dry with glycerine as a humectant wet in deionized water minimal (ultrapure quality) 4 - 16 2,5 10,2 0,1 0,81 0,5 ml to 30 no yes dry at room temperature or at 4 C in a polyethylene bag in original packing at 4 C approximately two years depending on storage conditions Universal Closures, Regular Closures, (Magnetic) Weighted Closures

18

Spectra/Por Biotech Dialysis Membrane Tubing Regenerated Cellulose (RC) Flat Width Vol./Length-ml/cm Diameter (mm) MWCO 3.500 8.000 10.000 15.000 25.000 4 mm 0,1 2,5 133 104 129 010 128 614 129 110 128 624 8 mm 0,25 5,1 133 108 129 012 128 618 132 606 128 628 10 mm 0,32 6,4 133 110 129 015 128 610 129 115 128 620 16 mm 0,81 10,2 133 116 129 020 128 616 129 120 128 626 Length (meter) 15 15 15 15 15

Spectra/Por Biotech Dialysis Membrane Tubing - Irradiated Regenerated Cellulose (RC) Flat Width Volume Diameter (mm) MWCO 8.000 15.000 8 mm 2 ml 5,1 130 106 130118 12 mm 5 ml 7,6 130 108 130 120 19 mm 10 ml 12,1 130 110 130 122 25 mm 20 ml 15,9 130 111 130 123 Length (meter) 15 15

Spectra/Por

Biotech Dialysis Membranes Tubing, Discs and Sheets. Cellulose Ester (CE)

Spectra/Por Cellulose Ester (CE) dialysis membranes offer an excellent Molecular Weight Cut Off (MWCO) selection for the separation of ionic species as well as macromolecules. The advanced manufacturing process provides CE dialysis membranes with an extremely high purity and a biologically inert surface to yield an optimum sample recovery. The Spectra/Por Biotech CE membranes are sensitive to organic solvents. Strong polar solvents such as acetone, MEK or dioxane will irrepairably damage the membrane. This membrane will tolerate exposure to lower alcohols (methanol, ethanol, and isopropanol) without apparent damage, with only slight change to the MWCO. The membrane should not be exposed to organic solvents (in water based solutions) at concentrations exceeding 5% The Biotech CE Membranes are hydrophilic and have uniform pore sizes. There is no special chemical precleaning required: just rinse the membrane in DI water before use.

MWCO range (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging: Packaging irradiated membranes: Trace elements contents: Flat width (mm): Tubing diameter (mm): Vol./Length-ml/cm: Sample volume range (ml): Discs/flat sheets: Wall thickness tubing (MWCO 100 50.000): Wall thickness tubing (MWCO 100.000): Wall thickness sheets: Available irradiated (sterile): Membrane storage: Irradiated membrane storage: Shelf life: Closure recommendation:

100 500 1.000 2.000 3.500 5.000 8.000 10.000 15.000 25.000 50.000 100.000 2 to 9 37 C opaque, rigid wet in 0,1% sodium azide wet in deionized water minimal (ultrapure quality) 8 31 5,1 to 19,7 0,2 3,1 0,5 30 yes 75 80 m for flat width 8 31 mm 100 125 m for flat width 8 31 mm 20 m for flat width 204 x 254 mm yes store wet membranes at 4 C in a preservative solution of 0,1% sodium azide in original packing at 4 C approximately two years depending on storage conditions Universal Closures (only)

19

Spectra/Por Biotech Dialysis Membrane Tubing Cellulose Ester (CE) Flat Width Vol./Length-ml/cm Diameter (mm) MWCO 100 500 1.000 2.000 3.500 5.000 8.000 10.000 15.000 25.000 50.000 100.000 8 mm 0,2 5,1 131 009 131 045 131 081 131 117 131 153 131 189 131 225 131 261 131 297 131 333 131 369 131 405 10 mm 0,32 6,4 131 012 131 048 131 084 131 120 131 156 131 192 131 228 131 264 131 300 131 336 131 372 131 408 12 mm 0,45 7,6 131 015 131 051 131 087 131 123 131 159 131 195 131 231 131 267 131 303 131 339 131 375 131 411 16 mm 0,81 10,2 131 018 131 054 131 090 131 126 131 162 131 198 131 234 131 270 131 306 131 342 131 378 131 414 24 mm 1,8 15,3 131 021 131 057 131 093 131 129 131 165 131 201 131 237 131 273 131 309 131 345 131 381 131 417 31 mm 3,1 19,7 131 024 131 060 131 096 131 132 131 168 131 204 131 240 131 276 131 312 131 348 131 384 131 420 Length (meter) 10 10 10 10 10 10 10 10 10 10 10 10

Spectra/Por

Biotech Dialysis Membranes Tubing, Discs and Sheets. Cellulose Ester (CE)

Spectra/Por Biotech Dialysis Membrane Tubing - Irradiated Cellulose Ester (CE) Flat Width Vol./Length-ml/cm Diameter (mm) MWCO 100 500 1.000 2.000 3.500 5.000 8.000 10.000 15.000 25.000 50.000 100.000 8 mm 0,2 5,1 130 400 130 420 130 440 130 460 130 480 130 500 130 520 130 540 130 560 130 580 130 600 130 620 10 mm 0,32 6,4 130 402 130 422 130 442 130 462 130 482 130 502 130 522 130 542 130 562 130 582 130 602 130 622 12 mm 0,45 7,6 130 404 130 424 130 444 130 464 130 484 130 504 130 524 130 544 130 564 130 584 130 604 130 624 16 mm 0,81 10,2 130 406 130 426 130 446 130 466 130 486 130 506 130 526 130 546 130 566 130 586 130 606 130 626 24 mm 1,8 15,3 130 408 130 428 130 448 130 468 130 488 130 508 130 528 130 548 130 568 130 588 130 608 130 628

Spectra/Por Biotech Dialysis Membrane Discs and Sheets Cellulose Ester (CE) Discs 33 MWCO 100 500 1.000 2.000 3.500 5.000 8.000 10.000 15.000 25.000 50.000 100.000 133 001 133 002 133 003 133 004 133 005 133 006 133 007 133 008 133 009 133 010 133 011 133 012 Discs 47 133 016 133 017 133 018 133 019 133 020 133 021 133 022 133 023 133 024 133 025 133 026 133 027 Discs 100 133 031 133 032 133 033 133 034 133 035 133 036 133 037 133 038 133 039 133 040 133 041 133 042 Sheets 203 x 254 133 080 133 081 133 082 133 083 133 084 133 085 133 086 133 087 133 088 133 089 133 090 133 091

Diameter (mm)

20

Spectra/Por

Biotech Dialysis Membranes Tubing Polyvinylidene Difluoride (PVDF)

PVDF membranes (Polyvinylidene Difluoride), represent a revolutionary advance in separation technology. The membranes, in contrast to typical cellulosic membranes, are hydrophobic and resistant to a wide variety of organic solvents as well as most aqueous acids and bases. These membranes can be heat sealed and autoclaved without affecting the Molecular Weight Cut Off (MWCO) or changing the membrane performance. Package of 10 meter length in 0,1% sodium azide preservative.

MWCO range (Daltons): pH range: Suggested temperature limit: Physical properties: Packaging: Trace elements contents: Flat width (mm) Tubing diameter (mm): Vol./Length-ml/cm: Sample volume range (ml): Discs/flat sheets: Available irradiated (sterile): Membrane storage: Shelf life: Closure recommendation:

250.000 500.000 1.000.000 Daltons 1 to 14 130 C opaque, rigid wet in 0,1% sodium azide minimal (ultrapure quality) 8 24 5,1 to 15,3 0,2 1,8 0,5 ml to 30 no no store wet membranes at 4 C in a preservative solution of 0,1% sodium azide approximately two years depending on storage conditions Universal Closures (only)

Spectra/Por Biotech Dialysis Membrane Tubing Polyvinylidene Difluoride (PVDF) Flat Width Vol./Length-ml/cm Diameter (mm) MWCO 250.000 500.000 1.000.000 8 mm 0,20 5,1 12 mm 0,45 7,6 16 mm 0,81 10,2 24 mm 1,8 15,3 Length (meter) 10 10 10

21

138 511 138 512 138 513

131 907 131 908 131 909

138 530 138 531 138 532

138 523 138 524 138 525

Spectra/Por

Dialysis Accessories

Spectra/Por Openers For easy opening of Spectra/Por Dialysis Tubing Solve the problem of opening dry dialysis tubing with Spectra/Por Openers. They have a strong adhesive that grips the dry tubing for easy membrane separation for filling with sample solution. The adhesive strips do not damage the membrane or do not have any influence on the dialysis process. Product number 132730 Description Spectra/Por Openers Quantity 100 per package

Cleaning Solutions Heavy Metals Cleaning Solution For ultra-critical work, wash the membranes with Spectra/Por Heavy Metal Cleaning Solution before use. In normal use, Spectra/Por membranes should be washed with distilled water and used without additional cleaning. However traces of heavy metal ions are present in Spectra/Por 1, 2, 3, 4, and 5 and for critical applications it is necessary to remove the heavy metals. In this case the Heavy Metals Solution is a very useful and easy tool.
Product number Description Quantity

132908

Heavy Metals Cleaning Kit

236 ml

Sulfide Removal Solution If sulfur interferes with your sample, the Sulfide Removal Solution will be your aid.

22

Spectra/Por 1, 2, 3, 4, 5 and 6 membranes have 0,1% sulphur present from manufacturing process which requires removal only if the presence might interfere with the subsequent analysis of the dialysate or retentate. Product number 132906 Description Sulfide Removal Kit - Solution A - Solution B Quantity 236 ml 236 ml

Closures NOTE 1 For the best result, choose a Closure that is 4 to 10 mm wider than the flat width of the tubing. This will allow the Closure to seal the tubing securely without tearing. NOTE 2 To use the right Closure for your dialysis tubing, please look at the MWCO tables.

Universal Closures Closure design ensures a reliable seal for all RC, CE and PVDF Membrane Tubing. This type of closure can be used to seal all types of Spectra/Por dialysis membrane tubing. The Universal Closure locks gently over rigid membranes and ensures a strong leak proof seal. Universal (Nylon) Closures sink on their own and allow the membrane tubing to float vertically with a headspace. There are 6 colours for easy sample identification and the large gripping widths eliminate using knots to close the large diameter tubes.

Spectra/Por

Dialysis Accessories

Specifications Sealing width: Materials: Maximum operating temperature: Minimum operating temperature: Sanitation:

50 mm, 70 mm, 110 mm, and 150 mm Polyamide 6.6 from Dupont (Nylon) 90 C 0 C Ethylene oxide Gamma irradiation Ethanol or Formaldehyde Non-autoclavable

Ordering information: Colour

Product Number for sealing Width (mm)

Quantity

White Red Yellow Blue Green Black

50 142150 142152 142153 142154 142155 142156

70 142170 142172 142173 142174 142175 142176

110 142110 142112 142113 -

150 142250 142252 142253 -

10 10 10 10 10 10

Spectra/Por Closures For use with Spectra/Por 1 through 7 Regenerated Cellulose Membranes and Biotech Regenerated Cellulose Membrane Tubing. Do not use with CE and PVDF membrane tubing. Spectra/Por Closures are lighter than water. They are normally used with a weighted Closure or a Magnetic Weighted Closure. Attach a regular Closure on the top of a dialysis sack and a Weighted Closure or Magnetic Weighted Closure on the bottom of the sack to automatically achieve a vertical floating position. Alternatively, use the glass weights (marbles) which are dropped into the dialysis sack. This maintains a vertical floating position for efficient dialysis.

23

Ordering information: Colour

Product Number for sealing Width (mm)

Quantity

Orange Naturel Green Blue

5 132733 142733 142833 142933

12 132734 142734 142834 142934

23 132735 142735 142835 142935

35 132736 142736 142836 142936

55 132737 142737 142837 142937

75 132738 142738 142838 142938

10 10 10 10

Product Number

Description

Quantity

132740

Glass Weights (marbles), diameter 15 mm average

50 per box

Spectra/Por

Dialysis Accessories

Spectra/Por Weighted Closures - To maintain a vertical floating position of RC Membrane Tubing. - Not for use with CE and PVDF membrane tubing. When a Weighted Closure is used in conjunction with a regular Closure, a vertical position of the dialysis tubing is achieved, increasing the rate of dialysis. Spectra/Por Weighted Closures are polypropylene and contain an acid-washed, fluorocarbon coated stainless steel bar imbedded on the back side. They are available 10/package or in combination packages with regular Closures, 20/package.

Ordering information: Product Number

Description

Sealing Width (mm)

Quantity

132741 132742 132743 132744 132745 132746 132748 132749 132750 132751 132752 132753

Weighted Closures Weighted Closures Weighted Closures Weighted Closures Weighted Closures Weighted Closures 10 Weighted Closures, 10 Closures 10 Weighted Closures, 10 Closures 10 Weighted Closures, 10 Closures 10 Weighted Closures, 10 Closures 10 Weighted Closures, 10 Closures 10 Weighted Closures, 10 Closures

5 12 23 35 55 75 5 12 23 35 55 75

10 10 10 10 10 10 20 20 20 20 20 20

24

Spectra/Por Magnetic Weighted Closures - Dialysis without a conventional Stir Bar - Not for use with CE or PVDF tubing The Magnetic Weighted Closure features an imbedded Teflon coated magnetic weight. The dialysis tubing submerses completely into the buffer chamber when used with a regular non-weighted closure. A slow spin movement of the dialysis tubing occurs when used in conjunction with a magnetic stir plate. No additional stir bar is required in order to maintain continuous motion of the dialysis tubing.
Ordering information: Product Number

Description

Sealing Width (mm)

Quantity

132760 132762 132764 132766

Magnetic Weighted Closure Magnetic Weighted Closure Magnetic Weighted Closure Magnetic Weighted Closure

23 35 55 75

2 per box 2 per box 2 per box 2 per box

Spectra/Por Dialysis Reservoir - Two reservoirs with different volumes provide an optimum environment for dialysis The 600 ml reservoir is recommended to accommodate Spectra/Por membrane tubing with a flat width of less than 16 mm and sealing closure width less than 75 mm. The 1.800 ml reservoir is recommended to accommodate any size membrane tubing with a flat width of less than 80 mm and sealing closure width less than 75 mm.
Ordering information: Product Number

Description

Vol. (ml)

Ht (cm)

Diameter (cm)

132002 132005

Dialysis Reservoir with Stir Bar Dialysis Reservoir with Stir Bar

600 1.800

12,5 30

11,5 11,5

CellMax Implants Membrane for Drug Discovery and Development

single CellMax hollow ber implant with encapsulated cells surgically implant

administer test compound

remove and test for cell viability

25

assay system

test for further drug potential

reject for drug potential

Hollow fiber implant technology for in vivo drug profiling greatly reduces the cost and time of xenographic animal studies while improving the science with the use of human cell lines: Screen compounds with Spectrum CellMax hollow fiber in vivo assay. Compounds with poor pharmacokinetics are rejected and compounds with pro-drug activity are accepted. For more information, please contact: Spectrum Europe B.V. P.O. Box 3262, 4800 DG Breda the Netherlands E-mail: info@spectrumeurope.nl www.spectrumeurope.nl

Membrane Compatibility Guide

This chemical resistance chart is intended for use as a guide, not as a guarantee of chemical compatibility. Variables in temperature, concentrations, durations of exposure and other factors may affect the use of the product. It is recommended to test under your own conditions. R L NR = Recommended = Limited Exposure Acceptable = Not recommended

PVDF

Acetic acid (diluted-5%) Acetic acid (med conc-25%) Acetic acid, glacial Acetone Acetonitrile Ammonium hydroxide (diluted) Ammonium hydroxide (med conc.) Amyl acetate Amyl alcohol Aniline Benzene Benzyl alcohol Boric acid Brine Bromoform Butyl acetate Butyl alcohol Butyl cellosolve Butylraldehyde Carbon tetrachloride Chloracetic acid Cellosolve Chloroform Cresol Cyclohexane Cyclohexanone Diacetone alcohol Dichloromethane Dimethyl formamide Dimethylsulfoxide 1,4 Dioxane Ethers Ethyl alcohol Ethanol, 15% Ethanol, 95%

L NR NR NR NR NR NR NR L NR NR NR R R NR NR L NR NR NR NR NR L NR L NR NR L NR NR NR NR L R L

R R R R R R L R R R R R R R R R R L R R R L R R R R R R L R R R R

R R R L R R R R R R R L R R R R R R R R R R R R L R R L R L R R R

Ethyl acetate Ethylene dichloride Ethylene glycol Ethylene oxide Formaldehyde, 2% Formaldehyde, 30% Formic acid, 25% Formic acid, 50% Freon Gasoline Glycerine Glycerol Hexane Hydrochloric acid, 5% Hydrochloric acid, 25% Hydrochloric acid, 37% Hydrofluoric acid, 25% Hydrogen peroxide, 30% Iodine solutions Isobutyl alcohol Isopropyl acetate Isopropanol Isopropyl acetate Isopropyl alcohol Isopropyl ether Jet Fuel 640A Kerosene Lactic acid Methyl acetate Methyl alcohol Methyl alcohol, 98% Methyl cellosolve Methyl chloride Methyl formate Methylethylketone

NR NR L NR L L NR NR R R R R R R

R R R L R R R R R R R R R R

26

NR NR NR NR NR R R NR L NR L L R R R NR L L L NR NR NR R R R R R R R R R R R R R L R L R

L NR R R R R R R R R R R R R R R L R L

NR NR NR

R NR

L NR

PVDF
R R R R R R R R R R R R R R R L

RC

RC

CE

CE

Membrane Compatibility Guide

PVDF

Methylisobutylketone Methylene chloride N-Methyl-2-Pyrrolidone Mineral Spirits Monochlorobenzene Nitric acid, 5% Nitric acid, 25% Nitric acid, 6N Nitric acid, concentrated Nitrobenzene Nitropropane Oils, mineral Pentane Perchloric acid, 25% Perchloroethylene Petroleum based oils Petroleum ether Phenol, 0.5% Phenol, 10% Phosphoric acid, 25% Potassium hydroxide, 1N Potassium hydroxide, 25% Potassium hydroxide, 50% Propanol Pyridine Silicone oil Sodium hydroxide, 0.1N Sodium hydroxide, 5%N Sodium hydroxide, 25% Sodium hydroxide, 50% Sodium hydroxide, concentrated Sodium hypochlorite Sulfuric acid, 5% Sulfuric acid, 25% Sulfuric acid, 6N

NR L NR R L L

R R R R R

L R R R R

Tetrahydrofuran Toluene Trichloroacetic acid, 25% Trichlorbenzene Trichlorethane Trichlorethylene Triethylamine Turpentine Urea Urea, 6N Water Xylene

NR R

R R

NR NR NR NR L R NR NR R NR R NR R R R R R R R

R NR R R R R R R R R R R R R R R R R L R R R R R R R R R R

R NR R R R R R R

NR NR NR NR NR NR NR R R NR NR R R R NR NR L NR R NR R L NR NR L L R R L R R R R R L L R R R R R L L NR NR NR

PVDF
R R R R R

RC

RC

CE

CE

27

NR NR

NR NR NR NR R L NR NR R R L L