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LECTURE PLAN DECEMBER 2012 APRIL 2013 BIO300: Biological Techniques and Skills WEEK*

TOPIC
Mini project proposal, thesis/report guidelines, general laboratory and fieldwork safety rules Techniques in Field Investigation 1.1 Introduction to field investigation 1.2 Safety rules in field investigation Draft Proposal Submission 1.3 The sampling techniques and sampling equipment 1.4 The preservation and mounting techniques in plants Proposal Submission MID-TERM BREAK (22nd -30th December 2012) 1.5 The preservation and mounting techniques in animals

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Techniques in Histology 2.1 Introduction to histology 2.2 Safety rules 2.3 Fixative chemicals 2.4 Histological preparation for tissues and cells Fixation, dehydration, embedding, sectioning, staining, mounting Test 1 Techniques in Microbiology 3.1 Introduction to microbiology 3.2 Safety rules 3.3 Microorganism control Sterilization Aseptic technique 3.4 Media preparation Type of media Bacterial cultures Draft Thesis Submission 1 (Methodology, Literature Review) 3.5 Microbiological analysis Viable cells analysis Total cells analysis 3.6 Preservation and storage FESTIVE SPECIAL HOLIDAY (9-17 February 2013)

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*Subject to change

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Techniques in Biochemical Analysis 4.1 Introduction to biochemical analysis 4.2 Safety rules 4.3 Extraction methods Test 2 4.4 Chromatography principles and techniques Thin Layer Chromatography (TLC) High Performance Liquid Chromatography (HPLC) Gas Chromatography (GC) Draft Thesis Submission 2 (Result, Discussion, Introduction) 4.5 Spectrophotometry Beers Law

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Techniques in DNA Technology 5.1 Introduction to DNA technology 5.2 Safety rules and bioethical 5.3 DNA Cloning and recombinant DNA Project Presentation 5.4 DNA Sequencing Conventional enzymatic sequencing Automated enzymatic sequencing 5.5 Polymerase Chain Reaction (PCR) Test 3 Final Thesis Submission Peer Evaluation REVISION WEEK (23-26 March 2013)

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Assessment: A continuous assessment will be conducted throughout the semester. A group of 3 to 4 students must completed ONE mini project as substitution of final exam. Mark distributions are as follow: Test 1 : Test 2 : Test 3 : Lab Report (6) : Laboratory skills : Project : i. Implementation : 20% ii. Report/Thesis : 20% iii. Presentation : 10% Total : 10% 10% 10% 10% 10% 50%

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Recommended Text Jones, A., Reed, R. and Weyers, J. (2003). Practical Skill in Biology (Third Edition). Great Britain: Pearson Prentice Hall. Wan Siti Atikah Wan Omar, Sarina Hashim and Nor Lailatul Wahidah Musa. (2012). Biological Techniques and Skills: A Laboratory Manual. Bandar Tun Abdul Razak Jengka, Pahang. Universiti Teknologi MARA Cawangan Pahang. References Campbell, N. A., Reece, J. B., Urry, L.A., Cain, M. L., Wasserman, S. A., Minorsky, P. V., and Jackson, R. B. (2008). Biology (Eight Edition). USA: Person Benjamin Cummings. Dubey, R.C and Maheswari D. K. (2006). Practical Microbiology (Revised Edition). New Delhi: S. Chand & Company LTD. Henderson, P. A. (2003). Practical Methods in Ecology. United Kingdom: Blackwell Publishing. Langford, A., Dean, J., Reed, R., Holmes, D., Weyers, J and Jones, A. (2005). Practical Skill in Forensic Science. Great Britain: Pearson Prentice Hall. Seidman, L. A. (2008). Basic Laboratory Calculations for Biotechnology. USA: Person Benjamin Cummings. Wan Ramlee, Sulaiman Shaari, Fauziah Ismail, Rusdin Laiman and Ismaniza Ismail. (2007). Guidelines on Supervision, Assessment, Evaluation and Format for Students' Project (Second Edition). Shah Alam: University Publication Centre (UPENA), Universiti Teknologi MARA.

Other Support Documents: Laboratory Report Template & Schedule Laboratory Skills and Report Grading Rubrics Mini Project Briefing Proposal Template Project Assessment/ Grading Rubrics

Its time to do some research

atikahomar2012- 2013/BIO300

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