TEST

METHOD

REAGENT USE; SUBSTRATE

END PRODUCT MEASURE COLOR; WAVELENGTH

- Pink color - @510 nm

NORMAL REFERENCE, CONVENTIONAL OR TRADITIONAL

- 70-110 mg/dL Or - 3.9-6.1 mmol/L

CONVERT ION FACTOR

S. I.

CLINICAL SIGNIFICANCE

REFERENCE METHOD

Trinders method GLUCOSE DETERMINATI ON Folin-wu method Ortho-Toulidine by Dubowski Nelsons Sumogyi method Modified Barthelot

-Glucose(Gl.) enzyme reagent; -Gl. Buffer

0.05551

mmol/L

Hexokinase Hyperglycemia Hypoglycemia To screen for, diagnose, and monitor high blood glucose (hyperglycemia) or low blood glucose (hypoglycemia), diabetes, and prediabetes Pre-ana Azotemia Renal azotemia Post-anal azotemia To evaluate kidney function in a wide range or circumstances, to help diagnose kidney disease, and to monitor patients with acute or chronic kidney dysfunction or failure. Hyperucemia Lesh nyan syndrome Gout The uric acid blood test is used to detect high levels of this compound in the blood in order to help diagnose gout. Diacetyl Monoxime by Fearon

BLOOD UREA NITROGEN

-Urea N-base Reagent -Urea N-zyme reagent; -Urea N-color reagent

- green color - @630 nm

- 8-23 mg/dL Or - 2.9-8.2 mmol/L

0.357

mmol/L

Trinder-uricase BLOOD URIC ACID Blauch and Koch method Catalase system Bittner method TPTZ (2,4,6tripyridyl-5triazine) by morin PAP(4aminophenazon) by Trinder

Uri-color reagent; Uri-zyme buffer -uricase -uricase -copper neocuprione (2,4,6-tripyrid-5triazine) -PAP -DHBS

- Yellow-orange - @520 nm - @290-300 nm - @410 nm - Deeply colored substance - blue - @590 nm - Red quinonemine derivative - @480-550 nm

Male 4-8.5 mg/dL 0.24-0.51 mmol/L

0.05948

mmol/L

Phosphotungstic Acid by caraway

Female 2.7-7.3 mg/dL 0.16-0.43 mmol/L

CREATANINE

Folin-wu method Jaffe Reaction

Alkaline picrate solution -alkaline picrate solution

- yellow color - @520 nm - creatinine picrate orange red color

-0.6-1.2 mg/dl -53-106 mol/L

88.40

mol/L

Enzymati/Trinder CHOLESTEROL Salkowski reaction

Cholesterol enzyme reagent; Cholesterol buffer -HAc -Fe(III)

- pink color - @510 nm Choloestapolyene carbonium ion - red color - choloestapolyene sulfonic acid -green color - Pink color - @520 nm

-150-250 mg/dL -3.88-6.47 mmol/L

0.0256

mmol/L

GFR Hyperthryoidism Diabetic acidosis Puerperium To determine if your kidneys are functioning normally and to monitor treatment for kidney disease Hypercholestenemia: CAD CVD Hypocholesteronemia Hemolytic anemia To screen for risk of developing heart disease

Jaffe reaction

Liebermannburchard reagent

LiebermannBurchard reagent HDL-C Enzymatic/Trinder

-acetic anhydrite -H2SO4 Cholesterol enzyme reagent; Cholesterol buffer

Male 23-63 mg/dL 6.76-16.38 mol/L Female 35-75 mg/dl 8.58-19.5 mmol/l -10-190 mg/dL -0.11-2.15 mmol/L

.26

mmol/L

The test for HDL cholesterol (HDL-C) is used along with other lipid tests to screen for unhealthy levels of lipids and to determine your risk of developing heart disease. Hypertriglycedemia Hypotriglycedemia Blood tests for triglycerides are usually part of a lipid profile used to identify the risk of developing heart disease. LDL cholesterol is used to predict risk of developing heart disease

Dextran sulfate magnesium

TRIGLYCERIDE

GPO-PAP METHOD-RANDOX

Cholesterol enzyme reagent; Cholesterol buffer

- Pink color - @500 nm

0.0114

mmol/L

Van Handell Zilmersmith method

LDL-C

Friedewald method

LDL-C = T.choles.-HDLTG/5 mg/dl = T.choles.-HDLTG/2.175 mmol/L

Optimal <100 mg/dl <2.59 mmol/l Near/above

.0259

mmol/L

Friedewald method

optimal - 100-129mgdl - 2.593.34mmol Borderline high - 130-159mg/dl -3.37-4.12mmol/l Very high - >189 - >4.9mol/l - 6-7.8 g/dL - 60-78 g/L

TOTAL PROTEIN

Biuret method

Bromcresol blue

Purple color @545 nm

10

g/L

ALBUMIN

Bromcresol green

Bromcresol green

Lime green color @628 nm

- 3.2-4.5 g/dL Or - 32-45 g/L

10

g/L

BILIRUBIN

Malloy and Evelyn method

Diazo reagent

Azobiliburin Red violet color @ 530 nm

-Direct - <0.3 mg/dL; or - <5 mol/ -Indirect - 0.1-1 mg/dL or - 2-17 mol/L -Total - 0.1-1.2

17.10

mol/L

Hyperproteinemia Hypoproteinemia Total protein measurements can reflect nutritional status may be used to screen for and help diagnose kidney disease, liver disease, and many other conditions. Hyperalbuminuria Hypoalbuminuria To screen for a liver disorder or kidney disease to evaluate nutritional status, especially in hospitalized patients, along with or instead of a pre-albumin test. Pre-hepatic Post-hepatic Hepatic jaundice Bilirubin is measured to diagnose and/or monitor liver diseases, such as cirrhosis, or gallstones

Biuret method

Bromcresol green

Malloy and Evelyn method

Sources for reference values:

mg/dL or 2-21 mol/L

Mc Person, R. A., Pincus, M. R. 2011. Henrys Clinical Diagnosis and Management by Laboratory Methods. Saunders. Burtis, C. A. & Ashwood, E. R. 2001. Tietz Fundamental of Clinical Chemistry. W. B. Saunders Company.