Beruflich Dokumente
Kultur Dokumente
http://www.inquisitr.com/451957/biologist-proposes-tohis-girlfriend-with-dna-fragments/
Gel electrophoresis
Separates macromolecules based on rate of movement through an agarose gel in an electric field. Distance travelled 1/length
What size of DNA molecules can be separated? How are the electrodes of the electrophoresis chamber oriented relative to the starting point of the DNA samples? How are DNA fragments separated by size?
Restriction enzymes/endonucleases
REs cut DNA molecules at a limited number of specific locations What are REs originally for? How is a bacterial cells own DNA protected from its REs? Terms: restriction site, restriction fragments, sticky end
www.goldiesroom.org
Restriction enzymes/endonucleases
Enzymes are named after the bacterium from which it is isolated
en.wikipedia.org
Which of the REs to the right produce sticky ends? Which produce blunt ends?
users.rcn.com
For this gel, those DNA fragments were obtained via PCR instead of using REs. PCwhat?
Theyre 5 sized PCR fragments (roughly 150, 300, 500, 700, 1kb), I went back through my notes to find 5 primer pairs that I knew worked pretty well (so dont feel bad, theyre selected out of primers that had been pre-validated =p). The other lanes are just mixes of the 5 sizes (either 2:3:4 or 4:6 volume mixes going in decreasing size, since larger fragments tend to be brighter). The gel actually didnt take that long (though it was terrifying loading it), but I made a mockup in Illustrator beforehand (along with a ladder to test what sizes to use), and then sketched it out beforehand so I knew what to add to each lane. - gdiguy2 (www.reddit.com/r/biology/comments/155fnj/there_is _literally_no_nerdier_way_to_propose/c7jnu8x)