LAPORAN TUGAS TERSTRUKTUR BIOLOGI MOLEKULER 2013 KELAS A ROMBONGAN II

NO NIM NAMA MAHASISWA

Learning Outcome (LO) LO 23: menjelaskan tahapan isolasi DNA dan RNA

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AZIZAH TIARA SUKMA

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FUJI MAGHFIROH

LO 24: menjelaskan cara purifikasi DNA dan RNA

TEXTBOX Cells have to be opened to enable nucleic acids to be isolated; opening cells should be done as gently as possible to avoid shearing large DNA molecules (35.1). Once broken open, cell preparations can be deproteinised and the nucleic acids purified by a range of techniques. Some applications require highly purified nucleic acid preparations; some may be able to use partially purified DNA or RNA (35.2). Solutions of nucleic acids are used to enable very small amounts to be handled easily, measured, and dispensed (35.3). Nucleic acids can be concentrated by using alcohol to precipitate the DNA or RNA from solution; the precipitate is recovered by centrifugation and can then be processed as required (36.1).

PENJELASAN TUGAS

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ANISA RAHMAWATI

LO 25: menjelaskan cara pengukuran kemurnian dan konsentrasi asam nukleat

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VANIA RAHMAYANTI

LO 26: Menjelaskan cara pemekatan asam nukleat

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ARIA RIZKI RAMADHAN

LO 27: menjelaskan jenis label dan cara pelabelan asam nukleat

Radioactive isotopes are often used to label nucleic acids, although they are more hazardous than nonradioactive labelling methods (38.1).

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MEDINA FADLI LATUS S

LO 28: menjelaskan aplikasi pelacak radioaktif

Radioactive probes are very useful for identifying specific DNA or RNA sequences (38.2).

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ANDIKA SETYOADI

LO 29: menjelaskan prinsip hibridisasi asam nukleat

The simple base-pairing relationship between complementary sequences has very far-reaching consequences both for the cell and its functioning and for the scientist who wishes to exploit this feature (39.2). Separation of biomolecules by gel electrophoresis is one of the most powerful techniques in molecular biology (40.1). The principles on which DNA sequencing is based are fairly simple; the procedures required to achieve the desired result are rather more complex (43.1).

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ATIKA TRI HANDAYANI

LO 30: menjelaskan prinsip elektroforesis gel dan tipe gel yang digunakan dalam elektroforesis

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NURUL ANISAH

LO 31: menjelaskan prinsip sekuensing DNA

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ANGGI PRATAMA

LO 31: menjelaskan prinsip sekuensing DNA

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ALIFATUN NA`IMAH

LO 32: menjelaskan preparasi fragmen DNA

Two rapid sequencing techniques were developed in the 1970s: the chemical method and the enzymatic method. Modern DNAsequencing technologies are based on the enzymatic method (43.2). The strategy employed to tackle large-scale sequencing projects depends on a number of factors, but essentially comes down to a choice between the ordered and shotgun approaches (44.1).