Kord Animal Health Diagnostic Laboratory KAHDL Ellington Agricultural Center 440 Hogan Road Nashville TN 37220 Phone:

(615) 837-5125 Fax: (615) 837-5250

Report Date: 4/5/2013

Final Report
Date Received: 3/25/2013 Collection Date: 3/25/2013 Case Coordinator: Millie D. Brookins, DVM

Accession No:

K1306468

Purpose of Test
Diagnostic

Animal Information
27126 ; CANINE

Client Report History

Report Type Preliminary Interim Interim Delivery Method Email Email Email Date Sent 3/27/2013 6:15:45 PM 3/28/2013 8:55:57 AM 4/1/2013 8:48:39 AM

Diagnosis Emaciation (Malnutrition/Starvation) Fat Atrophy, severe Muscle Atrophy Cutaneous Myiasis, generalized, moderate to severe (antemortem) Generalized Demodectic Mange, severe with bacterial dermatitis Stomach, Gastric Dilation with food overload (high appetite) Lymphadenopathy Pulmonary Edema Final Diagnosis Comment The most significant finding is the starvation which is the result of inadequate nutrition. There is no evidence of terminal infectious disease or malignant neoplasia that may cause the emaciation. Long periods of inadequate nutrition can have severe detrimental effects on the body including immune system impairment which is evident with the severe demodectic mange found in this dog; susceptibility to infections, prolonged gastric emptying and gastrointestinal transit times; and reduced cardiac output. There is no evidence of poisoning.
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Accession No: K1306468

The meat found next to the animal's body does not belong to the dog. Gross Examination The body and separate bag with a 45 X 5 X 6 cm piece of meat are received on 3/25/13. The necropsy is performed on 3/26/13. Presented is an approximately 4-5 year-old (based on teeth and tartar accumulation), spayed female Sheppard mix dog in poor body condition with moderate to severe postmortem autolysis. The dog has a blue collar but it is not embedded in the skin. On general examination, the body is generally covered with maggots and very thin with bony prominences on hips and ribs. The ventral skin is thickened and crusty. There are multiple open wounds (parasitic-like hole) as follow: right face, distal legs and right lateral thorax. The haircoat is dull and less abundant (no thick as in healthy dogs). There are some areas of alopecia on the legs with associated scabs. The rectum is missing (scavengers). The superficial inguinal lymph nodes are prominent. Upon opening the body and reflecting the skin, the subcutaneous tissue lacks of fat, and is green on some areas (autolysis). The internal organs are dark red, congested and generally decomposed. There is a severe lack of fat. The lung lobes are edematous. The stomach is distended and filled with abundant dry commercial dog food (food overload). The small intestine is empty. The distal large intestine is coated by small amounts of green pasty feces. The liver is slightly small (off fed). The spleen is not enlarged (no euthanized). The lymph nodes are generally enlarged (more over the subcutaneous LNs). No other significant gross findings are observed in the body. The piece of meat is slightly greenish and probably from a cow. There are not embedded pills. Photos are taken. Gross Examination Diagnosis Emaciation (Malnutrition/Starvation) Fat Atrophy, severe Muscle Atrophy Skin, Dermatitis, multifocal , severe with numerous fly larvae (myiasis) Mange Lymphadenopathy Pulmonary Edema Stomach, Gastric Dilation with food overload (high appetite)

Gross Examination Comments There is no evidence of trauma or fractures as judged by palpation. The emaciation is the result of inadequate intake of food. The skin condition is severe and sign of compromised immune system. Based on maggot maturing time, the dog has approximately 4-5 days death (this is just an estimation since maggots were not submitted to a forensic entomologist). The dog was not euthanized based on size of spleen. Histopathology Multiple tissues including brain, lung, liver, spleen, lymph node, kidney, liver, skin, urinary bladder and intestine are examined and generally autolytic. Sections from skin has large numbers of cross sections of mites within dilated hair follicles. The mites have jointed appendages; a thin, chitinous exoskeleton; and striated muscle. The epidermis is mildly hyperplastic with moderate ortho parakeratotic hyperkeratosis. The hair follicles also contain abundant cocci bacteria. There are aggregates of lymphocytes, plasma cells and neutrophils within the dermis. There is severe fat atrophy. There is no evidence of ethylene glycol crystals within the kidneys. The intestine and liver are markedly autolytic precluding examination. Remaining tissues are either autolytic or within normal limits. Lab Findings Bacteriology
Sample 27126 - CANINE Liver - Fresh Tissue - 1 Kidney - Fresh Tissue - 2 Aerobic Culture Pet (BCAERO-Pet) Aerobic Culture Pet (BCAERO-Pet) No Growth No Growth Escherichia coli Staphylococcus intermedius group Intestine - Swab/Culturette Aerobic Culture Pet (BCAERO-Pet) Escherichia coli <25 cfu <25 cfu >1000 cfu Test Name Organism Quantifier

Lymph Nodes - Fresh Tissue Aerobic Culture Pet (BCAERO-Pet) -3

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- Ellington Agricultural Center - Accession No: K1306468

Bacteriology
Sample 4 CFU is the abbreviation for colony forming units and reflects the estimated number of colonies present following incubation of culture plates. Test Name Organism Quantifier

Immunology
Sample 27126 - CANINE Animal-Dead - 1 Canine Distemper - FA (ICDVFA) Negative Sample 27126 - CANINE Animal-Dead - 1 Canine Parvovirus - FA (ICPVFA ) Negative Test Name Result Test Name Result

Parasitology
Sample Feces 2 Test Name Fecal Flotation (FECFLOT) Result 2+ / 2 to 4 eggs per low power field Comment 2+/ 2 to 4 organisms per low power field. Organisms consistent with Demodex canis 27126 - CANINE

Bulletin(s)

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- Ellington Agricultural Center - Accession No: K1306468