1) a) b) 2) a) b) 3) a) b) 4) a) b) 5) a) b)

The reagent developed by frederick sanger for protein sequencing is 1- fluoro-2,4 dintrobenzene Dansyl chloride c) dabsyl chloride d) phenylisothiocyanate

The concentration of HCl used in the protein sequencing is 5M c) 6 M 4M d) 7 M The instrument used for sequencing proteins automatically is NMR c) PCR Sequenator d) MALDI MS Which of the following bonds interfere in the protein sequencing Hydrophobic bonds c) disulphide bond Peptide bond d) hydrogen bond The enzyme trypsin cleavage of peptide is resulted in the presence of Glycine c) isoleucine Leucine d) lysine

6) The cyanogen bromide cleaves only those peptides bonds in which the carbonyl group is contributed by a) Arginine c) glutamate b) Methionine d) cysteine 7) During chemical synthesis of peptide, the amino group of the residue is protected from unwanted reactions by a) Dicyclohexylcarbodiimide c) dicylohexylurea b) 9- fluorenylmethoxycarbonyl d) urea 8) a) b) 9) a) b) The spatial arrangement of atoms in a proteins is called Conformation c) lattice Configuration d) isomerisation The length of a polypeptide with 80 amino acid residues in a single contiguous of helix is 135 c) 120 121 d) 150

10) Which instrument is used to determine the protein folding nature of the protein? a) Mass spectrometer c) CD spectroscopy b) NMR d) electron microscope 3

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11) Oxygen binds to iron atom in its a) Fe3+ b) Fe4+

c) Fe2+ d) Fe+

12) Which of the following compound is having greater affinity than oxygen towards heme iron? a) NH3 c) CO2 b) CO d) CH3 13) Which of the following protein regulates the muscle contraction? a) Desmin c) vimentin b) titins d) tropomyosin 14) Ecoli is a rod shaped bacteria about 2m long and 1 diameter.the average density of the cell is 1.24g/ml. approximately 13.5% of the wet weight of the cell is soluble protein. The no of molecules of a particular enzymes per cell if the enzyme has a MW of 100,000 (represents 0.1% of the total soluble protein) is a) 1262 molecules/ cell c) 1456 molecules/ cell b) 1626 molcules/ cell d)1545 molecules/ cell

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